• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Regeneration of the wound healing sites basing on the molecular analysis and the tissue-engineered

Research Project

Project/Area Number 13307051
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Plastic surgery
Research InstitutionNAGASAKI UNIVERSITY

Principal Investigator

FUJII Tohru  Nagasaki University, Graduate School of Biomedical and Sciences, professor, 大学院・医歯薬総合研究科, 教授 (60136661)

Co-Investigator(Kenkyū-buntansha) AKITA Sadanori  Nagasaki University, Medical School Hospital, professor, 医学部附属病院, 助手 (90315250)
HIRAO Akiyoshi  Nagasaki University, Graduate School of Biomedical and Sciences associate professor, 大学院・医歯薬総合研究科, 助教授 (90208835)
NAGAYAMA Yuji  Nagasaki University, Graduate School of Biomedical and Sciences associate professor, 大学院・医歯薬総合研究科, 助教授 (30274632)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
Fiscal Year 2002: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
Keywordskeloid / signal transduction / flap survival / mesenchymal stem cell / cutaneous wound healing / mesenchymal to epithelial differentiation / サイトカイン受容体操作 / 血管新生 / 幹細胞 / 初期増殖 / IGF-1 / TGF-β / ATF-2 リン酸化 / 分子生物学 / 細胞内情報伝達 / ケロイド / 骨再生 / サイトカイン
Research Abstract

For the successful regeneration of the wound healing sites, the molecular analysis and tissue-engineering methods are applied. Firat, the excessive wound healing such as keloids was analyzed with the signal transduction of the production of the extracelluar matrices. The TGF-./p38/ATF-2 signaling was most likely involved in the pathogenesis of the keloids. Then, the axial flap model was used for the possible cytokine and its receptor regulation on flap survival. The flap survival was significantly improved by cytokine-receptor modulation via flap axial vessels. The somatic stem cell in vitro investigation was focused on the early phase proliferation and differentiation. The cell proliferation, cell cycle regulation, histological findings and the ultrastructure of the bone marrow-derived human stem cells treated by basic fibroblast growth factor and bone morphogenetic protein-2 demonstrated the early phase growth of the human stemcells. Finally the in vivo use of the stem cell-mediated wound coverage was examined with an artificial skin substitute as a template. The stem cells treated with basic fibroblast growth factor were significantly healed in the nude rat skin defect model and the epithelization was suggested the stem cell-mediated mesenchymal to epithelial cell differentiation. The stem cells are beneficial for the cutaneous wound healing and possible clinical application for the difficult wound environment.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Akita S, Rashid MA, Ishihara H, Daian T, Dazai S, Fujii T: "Cytokine-dependent gp130 receptor subunit regulates rat modified axial-pattern epigastric flap"J Invest Surg. 15(3). 137-151 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Akita S, Nakagawa H, Akino K, Fukui M, Fujii T: "Stem Cells in Cutaneous Wound Healing"Journal des Plaies et Cicatrisations. (In Press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Daian T, Hirano A, Fujii T, et al.: "IGF-1 Enhances TGF-.-induced Extracellular Matrix Protein Production through the p38/ATF-2 Signaling Pathway in Keloid Fibroblasts"J Invest Dermatol. (In Press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 大浦武彦, 藤井徹, 宮地良樹, 藤井徹 他: "褥瘡危険因子とわかりやすい褥瘡予防・治療ガイドライン"日本医事新報. 4037. 19-29 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Akino K, Fujii T, Akita S, et al.: "Human mesenchymal stem cells regulate cell cycles and demonstrate the unique profile of the proliferation by bone morphogenetic protein-2"Wound repair and regeneration. (In Press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirano A, Suzuki H: "Factors related to relapse after Le Fort I maxillary advancement osteotomy in patients with cleft lip and palate"Cleft Palate-Cran J. 38(1). 1-10 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 平野明喜: "形成外科ADVANCEシリーズ II-9、骨延長術 最近の進歩 波利井清紀監修、杉原平樹編集"克誠堂出版. 230 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirano, A., Suzuki, H.: "Factors related to relapse after Le Fort I maxillary advancement oseotomy in patients with cleft lip and palate"Cleft Palate-Cran. J 38. 1-10 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Akita S., Ishihara H., Daian T., Fujii T., et al.: "Cytokine-dependent gp130 receptor subunit regulates rat modified axial-pattern epigastric flap"J Invest Surg.. 15. 137-151 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Akino K., Mineta T., Fukui M., Fujii T., Akita S.: "Human mesenchymal stem cells regulate cell cyclesand demonstrate the unique profile of the proliferation by bone morphogenetic protein 2"Wound Repair and Regeneration. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Daian T., Ohtsuru A., Rogounivitch T., Ishihara H., Hirano A., Akiyama-Uchida Y., Saenko V., Fujii T., Yamashita S.: "IGF-1 enhances TGF-. -induced extracellular matrix protein production through the p38/ATF-2 signaling pathway in keloid fibroblasts"J Invest Dermatol. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Akita S., Nakagawa H., Akino K., Fukui M., Fujii T.: "Stem cells in cutaneous would healing"Journal des Plaies at Cicatrisations. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Akita S, Rashid MA, Ishihara H, Daian T, Dazai S, Fujii T.: "Cytokine-dependent gp130 receptor subunit regulates rat modified axial-pattern epigastric flap"J Invest Surg. 15(3). 137-151 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Akita S, Nakagawa H, Akino K, Fukui M, Fujii T.: "Stem Cells in Cutaneous Wound Healing"Journal des Plaies et Cicatrisations. (in Press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Daian T, Hirano A, Fujii T, et al.: "IGF-1 Enhances TGH-β-induced Extracellular Matrix Protein Production through the p38/ATGF-2 Signaling Pathway in Keloid Fibroblasts"J Invest Dermatol. (in Press).

    • Related Report
      2002 Annual Research Report
  • [Publications] 大浦武彦, 藤井徹, 宮地良樹, 藤井徹 他: "褥瘡危険因子とわかりやすい褥瘡予防・治療ガイドライン"日本医事新報. 4037. 19-29 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 平野明喜, 鈴木弘之: "口唇裂、口蓋裂患者の顎骨骨切り術施行におけるチーム医療-形成外科と矯正歯科の立場より-"形成外科. 45巻3号. 223-230 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hirano A, Suzuki H: "Factors related to relapse after Le Fort I maxillary advancement osteotomy in patients with cleft lip and palate"Cleft Palate-Cran J. 38(1). 1-10 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 平野明喜: "形成外科ADVANCEシリーズII-9、骨延長術 細菌の進歩。波利井清紀監修、杉原平樹編集"克誠堂出版. 230 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hirano A, Suzuki H: "Factors related to relapse after LeFort I maxillary advancement osteotomy in patients with cleft lip and palate"Cleft Palate-Cran J. 38. 1-10 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Akita S, Ishihara H, Daian T, Dazai S, Fujii T., et al.: "Cytokine-dependent gp130 receptor subunit regulates rat modified axial-pattern epigastric flap"J Invest Surg. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] 堤 英博, 平野明喜, 秋田定伯, 藤井徹, 他: "Pyknodysostosisに対する上顎延長の経験"日本頭蓋学顔面外科学会誌. 17巻3号. 64-70 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nagayama Y, Nakao K, Eguchi K, Niwa M, et al.: "A novel murine model of Graves' hyperthyroidism with intramuscular injection of adenovirus expressing thyrotropin receptor"J Immunol. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Shklyaev SS, Nagayama Y, Yamashita S, et al.: "Involvement of wild-type p53 in radiation-induced c-Jun N-terminal kinase activation in human thyroid cells"Anticancer Res. 21. 2569-2576 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 田中克己, 藤井 徹, 村上隆一: "Emergency free flapの経験"日本マイクロサージャリー学会誌. 14. 190-199 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 平野明喜: "唇裂鼻の治療 臨床像と手術"荻野洋一、西村善彦、高戸毅 編集 克誠堂出版. 254 (2001)

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2002-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi