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The basic and clinical study on the effective individual oral cancer therapy

Research Project

Project/Area Number 13307057
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionMeikai University

Principal Investigator

SAKASHITA Hideaki  Meikai University, Dentistry, Professor, 歯学部, 教授 (10178551)

Co-Investigator(Kenkyū-buntansha) FUKUDA Masakatsu  Meikai University, Dentistry, Assistant Professor, 歯学部, 講師 (10311614)
SAKAGAMI Hiroshi  Meikai University, Dentistry, Professor, 歯学部, 教授 (50138484)
KUSAMA Kaoru  Meikai University, Dentistry, Professor, 歯学部, 教授 (20130479)
山本 美朗  明海大学, 歯学部, 教授 (40049383)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥43,160,000 (Direct Cost: ¥33,200,000、Indirect Cost: ¥9,960,000)
Fiscal Year 2003: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2002: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2001: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
Keywordsoral cancer / squamous cell carcinoma / gene alteration / p53 / apoptosis / anticancer drug / dendritic cell / VEGF
Research Abstract

1. The analysis of cytotoxic effect and its mechanism of apoptosis-induced drug for oral cancer cell lines. : The apoptosis-induced drug does not always reveal high tumor selectivity. Low-molecular-weight polyphenol inhibited NO production from LPS-stimulated macrophage.
2. Analysis of p53 gene alterations in primary oral cancer. : At first, immunohistochemistry using MAb p53 antibody was performed, 18 of 33 cases (54.5%) were positive. Then p53 gene alteration was analyzed by PCR-SSCP and direct sequencing. As the results, p53 point mutations were detected in 14 of 33 cases (42.4%). In addition to last year, p53 gene alterations were totally detected in 19 of 54 cases (35.2%).
3. Anticancer drug sensitivity test for individual patient. : Case1 : 5-FU : 78.2%, CDDP : 69.2 %, PEP : 0 %, TXL : 60 %, 254-S : 83.5% ; Case 2 : 5-FU : 53.2%, CDDP : 46.3%, PEP : 41.1%, TXL : 73.3%, 254-S : 68.8 % ; Case 3 : CDDP : 48.8%, PEP : 33.2%, TXL : 90.3%, 254-S : 67.9% ; Case 4 : We could not get informed concent ; Case 5 : Assay was failed. ; Case 6 : 5-FU : 37.8, CDDP : 27.1, 254-S : 52.9, TXL : 62, PEP : 3.9(%) ; Case 7 : CDDP : 27.1, PEP : 83(%)
4. The basic study on lymphocyte activating therapy. : Immortalization of cancer cells was not succeed yet. VEGF was detected from various oral cancer cell lines. The culture of Dendritic cells (DC) in the medium included VEGF inhibited the maturation of it. To inhibit the action of VEGF is a key way for the success of lymphocyte activating therapy.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] Fukuda M: "Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL/APO2L) and its receptors expression in human squamous cell carcinoma of the oral cavity"Oncology Reports. 10. 1113-1119 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakamura C: "Changes in intracellular concentrations of polyamines during apoptosis of HL-60 cells"Anticancer Research. 23(6). 4797-4804 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 菊池建太郎: "口腔癌と樹状細胞"Hosp.Dent.. 15(1). 23-31 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fukuda M: "A Patient with Multiple Primary Carcinomas, Including 4 Separate Oral Cancers : Study of p53 Mutations and their Implications for Management"J Oral Maxillofac Surg. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 福田正勝: "唾液腺悪性腫瘍におけるreceptor-binding cancer antigen expressed on SiSo cells(RCAS1)の発現とその機能について"消化器と免疫. 40(in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ide F: "p53 haploinsufficiency profoundly accelerates the onset of tongue tumors in mice lacking the xeroderma pigmentosum group A gene"Am J Pathol. 163(5). 1729-1733 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fukuda M.: "Tumor necrosis factor-related apoptosis-inducingligand (TRAIL/APO2L) and its receptors expression in human squamous cell carcinoma of the oral cavity."Oncology Reports. 10. 1113-1119 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakamura C.: "Changes in intracellular concentrations of polyamines during apoptosis of HL-60 cells."Anticancer Research. 23(6). 4797-4804 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kikuchi K.: "Oral cancer and dendritic cells."Hos Dent.. 15(1). 23-31 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fukuda M.: "A Patient with Multiple Primary Carcinomas, Including 4 Separate Oral Cancers : study of p53 Mutations and their Implications for Management."J Oral Maxillofac Surg. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fukuda M.: "The expression and its function of receptor-binding cancer antigen expressed on SiSo cells (RCAS1) in malignant salivary gland tumor."Digestive Organ and Mucosal Immunol.. 40(in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ide F.: "p53 haploinsufficiency profoundly accelerates the onset of tongue tumors in mice lacking the xeroderma pigmentosum group A gene."Am J Pathol.. 163(5). 1729-1733 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fukuda M: "Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL/APO2L) and its receptors expression in human squamous cell carcinoma of the oral cavity"Oncology Reports. 10. 1113-1119 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakamura C: "Changes in intracellular concentrations of polyamines during apoptosis of HL-60 cells"Anticancer Research. 23(6). 4797-4804 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 菊池建太郎: "口腔癌と樹状細胞"Hosp.Dent.. 15(1). 23-31 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Fukuda M: "A Patient with Multiple Primary Carcinomas, Including 4 Separate Oral Cancers : Study of p53 Mutations and their Implications for Management"J Oral Maxillofac Surg. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 福田正勝: "唾液腺悪性腫瘍におけるreceptor-binding cancer antigen expressed on SiSo cells (RCAS1)の発現とその機能について"消化器と免疫. 40(in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ide F: "p53 haploinsufficiency profoundly accelerates the onset of tongue tumors in mice lacking the xeroderma pigmentosum group A gene"Am J Pathol. 163(5). 1729-1733 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kikuchi K: "Dendritic cells in human squamous cell carcinoma of the oral cavity"Anticancer Res. 22(2). 545-558 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kusama K: "Vascular endothelial growth factor and dendritic cells in squamous cell carcinoma of the oral cavity"Dentistry in Japan. 38. 17-23 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Fukuda M: "Tumor necrosis factor-related apoptosis-inducing ligand(TRAIL/APO2L)and its receptors expression in human squamous cell carcinoma of the oral cavity"Oncology Rep.. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Kawase M: "3,5-dibenzoyl-1,4-dihydropyridines : Synthesis and MDR reversal in tumor cells"Bioorg Med Chem. 10. 1051-1055 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Watanabe K: "Cycloartane glycosides from the Rhizomes of Cimjicifuga racemosa and their cytotoxic activities"Chem Pharm Bull. 50. 121-125 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Taniguchi S: "Production of bioactive triterpenes by Eriobotyra Japonica calli"Phytochemistry. 59. 315-323 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Fukuda M: "Immunohistochemical detection of cytokeratin 18 and its neo-epitope in Warthin tumor(Adenolymphoma) of the parotid glands"Anticancer Res. 21. 109-112 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Asano K: "Tumor-specific action of sodium 5, 6-benzylidene-L-ascorbate in N-nitrosodiethylamine-administrated mouse model"Anticancer Res. 21. 281-284 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ide F: "Xeroderma pigmentosum group A gene action as a protection factor against 4-nitro quinoline 1-oxide-induced tongue carcinogenesis"Carcinogenesis. 22・4. 567-572 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ide F: "So-called hybrid odontogenic tumors : Do they really exist ?"Oral Med Pathol. 6・1. 13-22 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Aoki K: "Modification of apoptosis-inducing activity of gallic acid by saliva"Anticancer Res. 21. 1879-1884 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ide F: "Histopathology of ameloblastomas with emphasis on infrequent features"Hosp Dent (Tokyo). 13・2. 93-97 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Fukuda M: "Expression of neural cell adhesion molecule (NCAM) in human salivary gland tumor (HSG) cells"Hosp Dent (Tokyo). 13・2. 109-113 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Jiang Y: "Inhibition of chlorogenic acid-induced cytotoxicity by CoCl2"Anticancer Res. 21. 3349-3354 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 菊地建太郎: "口腔扁平上皮癌における原発巣と所属リンパ節での樹状細胞サブセットの動態-血管内皮増殖因子(VEGF)との関連性-"消化器と免疫. 38. 158-161 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 加賀谷雅之: "口腔癌に対する頸部郭清とリンパ節転移に関する臨床的研究"明海大学歯学雑誌. 30・2. 252-261 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kusama K: "Vascular endothelial growth factor and dendritic cells in squamous cell carcinoma of the oral cavity"Dentistry in Japan. 37(in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kikuchi K: "Dendritic cells in human squamous cell carcinoma of the oral cavity"Anticancer Res. 22(in press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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