• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The role of epithelial cells and fibroblasts in periodontal disease

Research Project

Project/Area Number 13307061
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Periodontal dentistry
Research InstitutionNihon University

Principal Investigator

OTSUKA Kichibee  Nihon University, School of Dentistry, Professor, 歯学部, 教授 (50059995)

Co-Investigator(Kenkyū-buntansha) MAEDA Katsumasa  Kyushu University, Graduate School of Dentistry, Professor, 大学院・歯学研究院, 教授 (00117243)
ABIKO Yoshimitsu  Nihon University, School of Dentistry at Matsudo, Professor, 松戸歯学部, 教授 (70050086)
KAWANAMI Masamitsu  Hokkaido University, Graduate School of Dentistry, Professor, 大学院・歯学研究科, 教授 (10133761)
SHIMAUCHI Hidetoshi  Tohoku University, Graduate School of Dentistry, Professor, 大学院・歯学研究科, 教授 (70187425)
IZUMI Yuichi  Kagoshima University, Graduate School of Dentistry, Professor, 大学院・歯学研究科, 教授 (60159803)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥53,950,000 (Direct Cost: ¥41,500,000、Indirect Cost: ¥12,450,000)
Fiscal Year 2003: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2002: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000)
Fiscal Year 2001: ¥31,460,000 (Direct Cost: ¥24,200,000、Indirect Cost: ¥7,260,000)
Keywordsperiodontal disease / epithelial cells / fibroblasts / chemotaxis / cytokine / signal transduction / integrin / immune response / 繊維芽細胞 / レセプター / 抗菌因子
Research Abstract

We studied the role of epithelial cells and fibroblasts in periodontal disease, and obtained the following results. 1) Laminin-8/9 (α4β1γ1/α4β2γ1) secreted by periodontal ligament fibroblast under serum-free culture were potent chemoattractant for gingival epithelial cells, and may be involved in apical migration of gingival epithelial cells. 2) Application of BMP-2 on root surface dose dependently induced alveolar bone regeneration in Beagle dogs. 3) Profiling of differentially expressed genes in human gingival epithelial cells and fibroblasts by DNA microarray revealed that epithelial cells highly expressed desmocollin, keratin-5, VAC-β, and fibroblasts highly expressed vimentin, gp130, and caveolin-2. 4) Tenascin-C, one of the extracellular matrix molecule, stimulated fibroblast multilayer formation, and may applicable for transplantation of fibroblast-containing sheet for periodontal tissue regeneration. 5) Rat palatal gingiva-derived epithelial cells express CD80, and induce T-cel … More l response when stimulated with IFN-γ and antigen through MHC class II and B-7. 6) P. gingivalis-stimulated mCD14-expressing gingival fibroblasts were activated through PTK, PKC, PKA/PKG and CaMK pathways, whereas sCD14 activated ibroblasts through PTK and PKC pathways revealed that fibroblast activation may depend upon mechanism of antigen recognition. 7) EMDOGAIN might not contain BMP-2, since it does not stimulate Cbfa-1 expression, ALPase activity, and mineralized nodule formation by periodontal ligament fibroblasts. 8) Hyaluronic acid metabolism of an established gingival fibroblast cell line, Gin-1, may regulated by HAS2 as a synthase, and HYAL2 and MGEA5 as degrading enzymes. 9) iNOS mRNA expression of human gingival epithelial cells was induced by IL-1β, TNF-α, adenosine RA (2CADO), or IL-15 stimulation. SV40-transformed gingival epithelial cells produced NO through iNOS mRNA upregulation when stimulated with 2CADO. Human gingival fibroblasts synthesized adenosine through CD73. 10) When HLA-antigen peptide-T cell receptor complex formed on fibroblast surface, production of RANTES and other cytokines were stimulated via JNK activation. 11) Downregulation of c-fos expression may be involved in cyclosporine A-induced gingival overgrowth in rats through decreased expression of α2 integrin which regulated by AP-1. 12) IFN-γ decreased collagen phagocytosis by gingival fibroblasts, however, cellular response of fibroblasts was not altered by collagen phagocytosis. Less

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] Shimabukuro Y, Murakami S, et al.: "Involvement of CD73 (ecto-5'-nucleotidase) in adenosine generation by human gingival fibroblasts."J Dent Res. Vol.82 No.11. 888-892 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yanagita M, Shimabukuro Y, et al.: "IL-15 up-regulates iNOS expression and NO production by gingival epithelial cells."Biochem Biophys Res Commun. Vol.297 No.2. 329-334 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Meguro M, Nishimura F, et al.: "Ligation of IFN-γ-induced HLA-DR molecules on fibroblasts induces RANTES expression via c-Jun N-terminal kinase (JNK) pathway."Cytokine. Vol.22 No.1. 107-115 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kataoka M, Nagata T, et al.: "Decreased expression of α2 integrin in fibroblasts isolated from cyclosporine A-induced gingival overgrowth."J Periodontal Res. Vol.38 No.5. 533-537 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kawase T, Okuda K, et al.: "Anti-TGF-β antibody blocks enamel matrix derivative-induced up-regulation of p2^<WAF1/cip1> and prevents its inhibition of human oral epithelial cell proliferation."J Periodontal Res. Vol.37 No.4. 255-262 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohshima M, Otsuka K, et al.: "Laminin- and fibronectin-like molecules produced by periodontal ligament fibroblasts under serum-free culture are potent chemoattractants for gingival epithelial cells."J Periodontal Res. Vol.37 No.2. 175-181 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shimabukuro Y, Murakami S, et al.: "Involvement of CD73 (ecto-5'-nucleotidase) in adenosine generation by human gingival fibroblasts"J Dent Res. 82(11). 888-892 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yanagita M, Shimabukuro Y, et al.: "IL-15 up-regulates iNOS expression and NO production by gingival epithelial cells"Biochem Biophys Res Commun. 297(2). 329-334 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Meguro M, Nishimura F, et al.: "Ligation of IFN-γ-induced HLA-DR molecules on fibroblasts induce RANTES expression via c-JUN N-terminal kinase (INK) pathway"Cytkine. 22(1). 107-115 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kataoka M, Nagata T, et al.: "Decreased expression of a2 integrin in fibroblasts isolated from cyclosporine A-induced gingival overgrowth"J Periodontal Res. 38(5). 533-537 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kawase T, Okuda K et al.: "Anti-TGF-β antibody blocks enamel matrix derivative-induced up-regulation of p21^<waf/cip1> and prevents its inhibition of human oral epithelial cell proliferation"J Periodontal Res. 37(4). 255-262 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kurosawa S, Abiko Y, et al.: "Characterization of rat monoclonal antibodies against human β-defensin-2"Hybrid Hybridomics. 21(5). 359-363 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Murakami S, Shimabukuro Y, et al.: "Activation of adenosine receptor enhanced iNOS mRNA expression by gingival epithelial cells"J Dent Res. 81(4). 236-240 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohshima M, Otsuka K, et al.: "Effects of serum on hepatocyte growth factor secretion and activation by periodontal ligament and gingival fibroblasts"J Periodontol. 73(5). 473-478 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohshima M, Otsuka K, et al.: "Laminin-and fibronectin-like molecules produced by periodontal ligament fibroblasts under serum-free culture are potent chemoattractants for gingival epithelial cells"J Periodontal Res. 38(2). 175-181 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shimabukuro Y, Murakami S., et al.: "Involvement of CD73(ecto-5'-nucleotidase) in adenosine generation by human gingival fibroblasts."J Dent Res. Vol.82No.11. 888-892 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yanagita M, Shimabukuro Y, et al.: "IL-15 up-regulates iNOS expression and NO production by gingival epithelial cells."Biochem Biophys Res Commun. Vol.297No.2. 329-334 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Meguro M, Nishimura F, et al.: "Ligation of IFN-γ-induced HLA-DR molecules on fibroblasts induces RANTES expression via c-Jun N-terminal kinase(JNK) pathway."Cytokine. Vol.22No.1. 107-115 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kataoka M, Nagata T, et al.: "Decreased expression of α2 integrin in fibroblasts isolated from cyclosporine A-induced gingival overgrowth."J Periodontal Res. Vol.38No.5. 533-537 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kawase T, Okuda K, et al.: "Anti-TGF-β antibody blooks enamel matrix derivative-induced up-regulation of p21^<WAF/cip1> and prevents its inhibition of human oril epithelial cell proliferation."J Periodontal Res. Vol.37No.4. 255-262 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ohshima M, Otsuka K, et al.: "Laminin- and fibronectin-like molecules produced by periodontal ligament fibroblasts under serum-free culture are potent chemoattractants for gingival epithelial cells."J Periodontal Res. Vol.37No.2. 175-181 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mitsuhiko Ohshima, Kichibee Otsuka, et al.: "Effects of serum on hepatocyte growth factor secretion and activation by periodontal ligament and gingival fibroblasts"J. Periodontol.. Vol.73 No.5. 473-478 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mitsuhiro Ohshima, Kichibee Otsuka, et al.: "Laminin-and fibronectin-like molecules produced by periodontal ligament fibroblasts under serum-free culture are potent chemoattractants for gingival epithelial cells"J. Periodontal Res.. Vol.38 No.2. 175-181 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mitsuhiko Ohshima, Kichibee Otsuka, et al.: "Physiologic levels of epidermal growth factor in saliva stimulate cell migration of an oral epithelial cell line, HO-1-N-i"Eur. J. Oral Sci.. Vol.110 No. 2. 130-136 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.Kurosawa, M.Ohta, Y.Abiko et al.: "Characterization of rat monoclonal antibodies against human β-defensin-2."Hybridoma and Hybridomics. Vol.22. 359-363 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Kawase, K.Okuda, H.Yoshie, DM.Burns: "Anti-TGF-β antibody blocks enamel matrix derivative-induced up-regulation of p21WAF1/cip1 and prevents its inhibition of human oral epithelial cell proliferation"J. Periodontal Res.. Vol.37 No.4. 255-262 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.Murakami, Y.Yoshimura, Y.Shimabukuro, H.Okada, et al.: "Activation of adenosine receptor enhanced iNOS mRNA expression by gingival epithelial cells"J. Dent. Res.. Vol.81 No.4. 236-240 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mitsuhiro Ohshima, Kichibee Otsuka, et al.: "Effects of serum on hepatocyte growth factor secretion and activation by periodontal ligament and gingival fibroblasts"J.Periodontol.. Vol.73(in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Mitsuhiro Ohshima, Kichibee Otsuka, et al.: "Laminin- and fibronectin-like molecules produced by periodontal ligament fibroblasts under serum-free culture are potent chemoattractants for gingival epithelial cells"J.Periodont.Res.. Vol.37(in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Mitsuhiro Ohshima, Kichibee Otsuka, et al.: "Physiologic levels of epidermal growth factor in saliva stimulate cell migration of an oral epithelial cell line,HO-1-N-1"Eur.J.Oral Sci.. Vol.110(in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 野崎弘晃, 大島光宏, 山口洋子, 小木曽文内, 明石俊和, 大塚吉兵衛: "PGE_2が歯根嚢胞由来細胞の骨吸収性サイトカイン産生におよぼす影響"日歯保存誌. 44巻5号. 836-843 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 野崎弘晃, 大島光宏, 山口洋子, 小木曽文内, 明石俊和, 大塚吉兵衛: "PGE_2が歯根嚢胞由来細胞のケラチノサイト増殖因子(KGF)産生に及ぼす影響"日歯保存誌. 44巻6号. 917-923 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] S.Murakami, Y.Yoshimura, Y.Shimabukuro, H.Okada, et al: "Activation of adenosine receptor enhanced iNOS mRNA expression by gingival epithelial cells"J.Dent.Res.. Vol.81(in press). (2002)

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi