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Structure and function of novel pumps and channels in gastrointestinal tract

Research Project

Project/Area Number 13307063
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical pharmacy
Research InstitutionTOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY

Principal Investigator

TAKEGUCHI Noriaki  Toyama Medical and Pharmaceutical University, Vice president, 副学長 (00019126)

Co-Investigator(Kenkyū-buntansha) SAKAI Hideki  Toyama Medical and Pharmaceutical University, Faculty of Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 (60242509)
ASANO Shinji  Toyama Medical and Pharmaceutical University, Life Science Research Center, Associate Professor, 生命科学実験センター, 助教授 (90167891)
HATANAKA Yasumaru  Toyama Medical and Pharmaceutical University, Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (30111181)
MORII Magotoshi  Toyama Medical and Pharmaceutical University, Faculty of Pharmaceutical Sciences, Lecturer, 薬学部, 講師 (60019130)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥37,960,000 (Direct Cost: ¥29,200,000、Indirect Cost: ¥8,760,000)
Fiscal Year 2003: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2002: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2001: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Keywordsgastric proton pump / gastric acid secretion / gastric flippase / proton pump inhibitor / chloride channel / cytoprotection / interleukin / nitric oxide / 大腸プロトンポンプ / 胃フリツパーゼ
Research Abstract

1.We succeeded to construct a stable cell line(polarized LLC-PK1 cell) expressing the gastric proton pump α-and β-subunits. By using this cell line, we found for the first time that the gastric proton pump functions as phospholipid flippase.
2.In the studies using HEK-293 cells expressing gastric proton pump, we found that the amino acid residues important for the proton pump function in the M6 segment are exclusively localized on the one side of the putative α-helical structure. We also found that all three disulfide bonds in the ectodomain of the β-subunit are necessary for the α-β assembly, proton pump activity, stability of the α-and β-subunits, and cell surface delivery of the α-subunit.
3.We succeeded to establish conditionally immortalized gastric mucosal cell lines expressing mRNA for gastric proton pump from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen.
4.We found that IL-1β binds to the IL-1 receptor of rabbit gastric parietal cells and inhibits the basolateral housekeeping Cl^-channel via G protein-mediated Rho/Rho-kinase-dependent production of O_2^-. CLCA1 is not a subunit of the gastric housekeeping Cl^-channel.
5.We found that the NO-dependent novel mechanism of activation of Cl^-channels in rat colonic mucosa. In this mechanism, NO stimulates the production of thromboxane A_2, and the released thromboxane A_2 activates the Cl^-channels in colonic crypt cells.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (74 results)

All Other

All Publications (74 results)

  • [Publications] Sakai H.et al.: "Is rabbit CLCA1 related to the basolateral Ca^<2+>-dependent Cl^- channel of gastric parietal cells?"Jpn.J.Physiol.. 51. 121-125 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Asano S.et al.: "Alanine-scanning mutagenesis of the sixth transmembrane segment of gastric H^+,K^+-ATPase α-subunit."J.Biol.Chem.. 276. 31265-31273 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tabuchi Y.et al.: "Cibenzoline, an ATP-sensitive K^+ channel blocker, binds to the K^+-binding site from the cytoplasmic side of gastric H^+,K^+-ATPase."Br.J.Pharmacol.. 134. 1665-1662 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hashimoto M.et al.: "Synthesis of biotinylated bis (D-glucose) derivatives for glucose transporter photoaffinity labeling."Carbohydr.Res.. 331. 119-127 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakai et al.: "Molecular and pharmacological properties of inwardly rectifying K^+ channels of human lung cancer cells."Eur.J.Pharmacol.. 435. 125-133 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujita A.et al.: "Specific localization of an inwardly rectifying K^+ channel, Kir4.1,at the apical membrane of rat gastric parietal cells ; its possible involvement in K^+ recycling for the H^+-K^+-pump."J.Physiol.. 540. 85-92 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kimura T.et al.: "Mutational study on the roles of disulfide bonds in the β-subunit of gastric H^+,K^+-ATPase."J.Biol.Chem.. 277. 20671-20677 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tabuchi Y.et al.: "Identification of genes responsive to sodium butyrate in colonic cells."Biochem Biophys.Res.Commun.. 293. 1287-1294 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakai H.et al.: "E3040 sulfate, a novel thromboxane synthase inhibitor, blocks the Cl^- secretion induced by platelet-activating factor in isolated rat colon."Br.J.Pharmacol.. 136. 383-390 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakai H.et al.: "Nitric oxide-induced Cl^- secretion in isolated rat colon is mediated by the release of thromboxane A_2."J.Physiol.. 543. 261-271 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tabuchi Y.et al.: "Development and characterization of conditionally immortalized gastric epithelial cell lines from transgenic rats harboring temperature-sensitive simian virus 40 large T-antigen gene."Cell Struct.Funct.. 27. 71-79 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kimura T. et al.: "Stable expression of gastric proton pump activity at the cell surface."J.Biochem.. 131. 923-932 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takahashi T.et al.: "Expression of ATP1AL1,a non-gastric proton pump, in human colorectum."Jpn.J.Physiol.. 52. 317-321 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hashimoto M.et al.: "Improvement in the properties of 3-phenyl-3-trifluoromethyldiazirine based photoreactive bis-glucose probes for GLUT4 following substitution on the phenyl ring."Chem.Pharm.Bull.. 50. 1004-1006 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sadakane Y.et al.: "Protein domain of chicken α_1-acid glycoprotein is responsible for chiral recognition."Biochem.Biophys.Res.Commun.. 295. 587-590 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yang J.et al.: "Development of high affinity ligands and photoaffinity labels for the D-fructose transporter GLUT5."Biochem.J.. 367. 533-539 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tabuchi Y.et al.: "New gastric epithelial cell lines from mice transgenic for temperature-sensitive simian virus 40 large T antigen show distinct types of cell differentiation."Digestion. 67. 71-81 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Asano S.et al.: "Quality control of gastric proton pump in the endoplasmic reticulum by ubiquitin/proteasome system."Ann.N.Y.Acad.Sci.. 986. 655-657 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kimura T.et al.: "Quantity and quality control of gastric proton pump in the endoplasmic reticulum by ubiquitin/proteasome system."Biochemistry. 42. 4771-4779 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakai H.et al.: "Inhibition of small-conductance Cl^- channels by the interleukin-1β-stimulated production of superoxide in rabbit gastric parietal cells."J.Physiol.. 551. 207-217 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sadakane Y.et al.: "Quantification of the isomerization of Asp residue in recombinant αA-crystallin by reversed-phase HPLC."J.Pharm.Biomed.Anal.. 30. 1825-1833 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Asano S.et al.: "The cavity structure for docking the K^+-competitive inhibitors in the gastric proton pump."J.Biol.Chem.. 279. 13968-13975 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakai H.et al.: "Leukotrienes-mediated effects of water extracts from Sargassum horneri, a marine brown alga, on Cl^- absorption in isolated rat colon."Jpn.J.Physiol. 54. 71-77 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakai H.et al.: "Up-regulation of Na^+,K^+-ATPase α3-isoform and down-regulation of the α1-isoform in human colorectal cancer."FEBS Lett.. 563. 151-154 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Asano S.et al.: "Molecular and cellular regulation of the gastric proton pump."Biol.Pharm.Bull.. 27. 1-12 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 竹口 紀晃: "「疾病と病態生理」1.消化器疾患"南江堂. 8 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakai H. et al.: "Is rabbit CLCA1 related to the basolateral Ca^<2+>-dependent Cl^-channel of gastric parietal cells?"Jpn.J.Physiol.. 51. 121-125 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Asano S. et al.: "Alanine-seanning mutagenesis of the sixth transmembrane segment of gastric H^+,K^+-ATPase α-subunit."J.Biol Chem.. 276. 31265-31273 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tabuchi Y. et al.: "Cibenzoline, an ATP-sensitive K^+ channel blocker, binds to the K^+-binding site from the cytoplasmic side of gastric H^+,K^+-ATPase."Br.J.Pharmacol.. 134. 1655-1662 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hashimoto M. et al.: "Synthesis of biotinylated bis(D-glucose) derivatives for glucose transporter photoaffinity labeling."Carbohydr.Res.. 331. 119-127 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakai H. et al.: "Molecular and pharmacological properties of inwardly rectifying K^+ channels of human lung cancer cells."Eur.J.Pharmacol.. 435. 125-133 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujita A. et al.: "Specific localization of an inwardly rectifying K^+ channel, Kir4.1, at the apical membrane of rat gastric parietal cells ; its possible involvement in K^+ recycling for the H^+-K^+-pump."J.Phystol.. 540. 85-92 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kimura T. et al.: "Mutational study on the roles of disulfide bonds in the β-subunit of gastric H^+, K^+-ATPase."J.Biol Chem.. 277. 20671-20677 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tabuchi Y. et al.: "Identification of genes responsive to sodium butyrate in colonic cells."Biochem Biophys.Res.Commun.. 293. 1287-1294 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakai H. et al.: "E3040 sulfate, a novel thromboxane synthase inhibitor, blocks the Cl^-secretion induced by platelet-activating factor in isolated rat colon."Br.J.Pharmacol.. 136. 383-390 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakai H. et al.: "Nitric oxide-induced Cl^-secretion in isolated rat colon is mediated by the release of thromboxane A_2."J.Physiol.. 543. 261-271 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tabuchi Y. et al.: "Development and characterization of conditionally immortalized gastric epithelial cell lines from transgenic rats harboring temperature-sensitive simian virus 40 large T-antigen gene."Cell Struct.Funct.. 27. 71-79 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kimura T. et al.: "Stable expression of gastric proton pump activity at the cell surface."J.Biochem.. 131. 923-932 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takahashi Y. et al.: "Expression of ATP1AL1, a non-gastric proton pump, in human colorectum."Jpn.J.Physiol.. 52. 317-321 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hashimoto M. et al.: "Improvement in the properties of 3-phenyl-3-trifluoromethyldiazirine based photoreactive bis-glucose probes for GLUT4 following substitution on the phenyl ring."Chem.Pharm.Bull.. 50. 1004-1006 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sadakane Y. et al.: "Protein domain of chicken α_1-acid glycoprotein is responsible for chiral recognition."Biochem Biophys.Res.Commun.. 295. 587-590 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yang J.et al.: "Development of high affinity ligands and photoaffinity labels for the D-fructose transporter GLUTS."Biochem.J.. 367. 533-539 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tabuchi Y. et al.: "New gastric epithelial cell lines from mice transgenic for temperature-sensitive simian virus 40 large T antigen show distinct types of cell differentiation."Digestion. 67. 71-81 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Asano S. et al.: "Quality control of gastric proton pump in the endoplasmic reticulum by ubiquitin/proteasome system."Ann.N.Y.Acad.Scl.. 986. 655-657 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kimura T. et al.: "Quality and quantity control of gastric proton pump in the endoplasmic reticulum by ubiquitin/proteasome system."Biochemistry. 42. 4771-4779 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakai H. et al.: "Inhibition of small-conductance Cl^-channels by the interleukin-1β-stimulated production of superoxide in rabbit gastric parietal cells."J.Phystol.. 551. 207-217 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sadakane Y. et al.: "Quantification of the isomerization of Asp residue in recombinant αA-crystallin by reversed-phase HPLC."J.Pharm.Biomed.Anal.. 30. 1825-1833 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Asano S. et al.: "The cavity structure for docking the K^+-competitive inhibitors in the gastric proton pump."J.Biol Chem.. 279. 13968-13975 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakai H. et al.: "Leukotrienes-mediated effects of water extracts from Sargassum horneri, a marine brown alga, on Cl^-absorption in isolated rat colon."Jpn.J.Physiol.. 54. 71-77 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakai H. et al.: "Up-regulation of Na^+,K^+-ATPase α3-isoform and down-regulation of the al-isoform in human colorectal cancer."FEBS Lett.. 563. 151-154 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Asano S. et al.: "Molecular and cellular regulation of the gastric proton pump."Biol. Pharm.Bull.. 27. 1-12 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tabuchi Y, Arai Y, Shioya H, Kuribayashi R, Ishibashi K, Sugiyama N, Obinata M, Takeguchi N, Asano S.: "New gastric epithelial cell line from mice transgenic for temperature-sensitive simian virus 40 large T-antigen show distinct types of cell differentiation"Digestion. 67・1-2. 71-81 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kimura T, Ishizuka H, Yoshida A, Morii M, Takeguchi N, Asano S.: "Quality and quantity control of gastric proton pump in the endoplasmic reticulum by ubiquitin/proteasome system"Biochemistry. 42・17. 4771-4779 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sakai H, Ohira Y, Tanaka A, Suzuki T, Ikari A, Morii M, Takeguchi N.: "Inhibition of small-conductance Cl^- channels by the interleukin-1β-stimulated production of superoxide in rabbit gastric parietal cells"Journal of Physiology. 551・1. 207-217 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Asano S, Yoshida A, Yashiro H, Kobayashi Y, Morisato A, Ogawa H, Takeguchi N, Morii M.: "The cavity structure for docking the K^+-competitive inhibitors in the gastric proton pump"Journal Biological Chemistry. (In press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sakai H, Uchiumi T, Lee J-B, Ohira Y, Okura J, Suzuki T, Hayashi T, Takeguchi N.: "Leukotrienes-mediated effects of water extracts from Sargassum horneri, a marine brown alga, on Cl^- absorption in isolated rat colon"Japanese Journal of Physiology. (In press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sakai H, Suzuki T, Maeda M, Takahashi Y, Horikawa N, Minamimura T, Tsukada K, Takeguchi N.: "Up-regulation of Na^+, K^+, ATPase α3-isoform and down-regulation of the α1-isoform in human colorectal cancer"FEBS Letters. (In press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Fujita A et al.: "Specific localization of an inwardly rectifying K^+ channel, Kir4.1, at the apical membrane of gastric parietal cells ; its possible involvement in K^+ recycling for activation of H^+-K^+-pump"J. Physiol. (London). 540. 85-92 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kimura T et al.: "Mutational study on the roles of disulfide bonds in the β-subunit of gastric H^+,K^+-ATPase"J. Biol. Chem.. 272. 20671-20677 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kimura T et al.: "Stable expression of gastric proton pump activity at the cell surface"J. Biochemistry. 131. 923-932 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takahashi Y. et al.: "Expression of ATP1AL1, a non-gastric proton pump, in human colorectum"Jpn. J. Physiol.. 52. 317-321 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sakai H. et al.: "E3040 sulphate, a novel thromboxane synthase inhibitor, blocks the Cl^-secretion induced by platelet-activating factor in isolated rat colon"Br. J. Pharmacol.. 316. 383-390 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sakai H. et al.: "Nitric oxide-induced Cl^-secretion in isolated rat colon is mediated by the release of thromboxane A_2"J. Physiol. (London). 543. 261-271 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Asano S., et al.: "Alanine-scanning mutagenesis of the sixth transmembrane segment of gastric H, K-ATPase α-subunit."Journal of Biological Chemistry. 276. 31265-31273 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tabuchi Y., et al.: "Cibenzoline, an ATP-sensitive K channel blocker, binds to the K-binding site from the cytoplasmic side of gastric H, K-ATPase."British Journal of Pharmacology. 134. 1655-1662 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sakai H., et al.: "Molecular and pharmacological properties of inwardly rectifying K channels of human lung cancer cells."European Journal of Pharmacology. 435. 125-133 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sakai H., et al.: "Is rabbit CLCA1 related to the basolateral Ca-dependent CI channel of gastric parietal cells?"Japanese Journal of Physiology. 51. 121-125 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kimura T. etal.: "Mutational study on the roles of disulfide bonds in the beta-subunit of gastric H+, K+-ATPase."Journal of Biological Chemistry. 277(In press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tabuchi Y., et al.: "Development and characterization of conditionally immortalized gastric epithelial cell lines from transgenic rats harboring temperature-sensitive simian virus 40 large T-antigen gene."Cell Structure and Function. (In press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hatanaka Y., et al.: "A Model of Photoprobe Docking with β1, 4- Galactosyltransferase Identify a Possible Carboxylate Involved in Glycosylation Steps."Bioorganic Medicinal Chemistry. 11. 411-413 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hashimoto M. et al.: "Synthesis of Biotinylated Bis (D-Glucose) Derivatives for Glucose Transporter Photoaffinity Labelling."Carbohydrate Research. 311. 119-127 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hashimoto M., et al.: "Hashimoto M., Hatanaka Y., Nabeta K., Versatile synthesis of phenoxydiazirine based fatty acid analogues and photoreactive galactosylceramide."Bioorganic Medicinal Chemistry Letters. 12. 89-91 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hatanaka Y., Sadakane Y.: "Photoaffinity Labeling in Drug Discovery and Developments : Chemical Gateway for Entering Proteomic Frontier."Current Topics in Medicinal Chemistry. 2. 271-288 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 竹口紀晃: "「疾病と病態生理」1.消化器疾患"南江堂. 8 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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