• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular Genetic Studies on the Physiological Function and Disorders of DNA Methylation

Research Project

Project/Area Number 13307067
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Human genetics
Research InstitutionNational Institute of Genetics

Principal Investigator

SASAKI Hiroyuki  National Institute of Genetics, Department of Integrated Genetics, Professor, 総合遺伝研究系, 教授 (30183825)

Co-Investigator(Kenkyū-buntansha) NAKAO Mitsuyoshi  Kumamoto University, Institute of Molecular Embryology and Genetics, Professor, 発生医学研究センター, 教授 (00217663)
SADO Takashi  National Institute of Genetics, Department of Integrated Genetics, Assistant Professor, 総合遺伝研究系, 助手 (70321601)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥54,470,000 (Direct Cost: ¥41,900,000、Indirect Cost: ¥12,570,000)
Fiscal Year 2003: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2002: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2001: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
KeywordsDNA methylation / DNA methyltransferases / methyl-CpG binding proteins / ICF syndrome / Rett syndrome / DNAメチル化 / レット症侯群
Research Abstract

Methylation of CpG dinucleotides plays a central role in the epigenetic genome regulation, and its defects cause congenital disorders and cancers. We have studied the functions and disorders of DNA methyltransferases (DNMTs) and methyl-CpG-binding proteins (MBD proteins) and found the followings. 1.By knocking out mouse DNMT3A, a de novo-type. DNMT, in a germline-specific manner, we found that this enzyme is essential for paternal and maternal imprinting. 2.Enzymatic properties and target specificities of DNMT3A and DNMT3B were determined. 3.New mutations of DNMT3B were identified in Japanese families with ICE syndrome. We also found an ICE case with no mutation in DNMT3B, which suggests the heterogeneity of the disease. 4.We reported that paternal disomy 14 is a new imprinting-related disorder characterized by bell-shaped chest and wavy ribs. 5.We found that MBD1 represses transcription and forms repressive chromatin through two independent pathways: 1) one mediated by a complex formed with a transcriptional mediator MCAF/AM and a histone methyltransferase SETDB1/ESET; 2) the other involving a histone methyltransferase Suv39, a histone deacetylase HDAC1/2 and a heterochromatin protein HP1. 6.The tertiary structure of the DNA binding domain of MBD1 complexed with a. methylated-CpG-containing DNA molecule was determined. 7.The mutations of McCP2 causing Rett syndrome were found to impair its ability to repress transcription and to form repressive chromatin. 8.We reported that MBD1 promotes base excision repair in cooperation with a methylated-purine DNA glycosylase. All together, our findings have greatly increased our knowledge on the mechanisms of DNA methylation and transcriptional repression through it and the pathology of DNA methylation-associated disorders.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (92 results)

All Other

All Publications (92 results)

  • [Publications] Mizuno, S.: "Expression of DNA methyltransferases DNMT1, 3A and 3B in normal hematopoiesis and in acute and chronic myelogenouse leukemia."Blood. 97. 1172-1179 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Watanabe, S.: "Methylated DNA-binding domain 1 and methylpurine-DNA glycosylase links transcriptional repression and DNA repair in chromatin"Proc.Natl.Acad.Sci.USA. 100. 12859-12864 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohno, M.: "Allele-specific detection of nascent transcripts by fluorescence in situ hybridization reveals temporal and culture-induced changes in Igf2 imprinting during pre-implantation mouse development."Genes Cells. 6. 249-259 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sado, T.: "Regulation of imprinted X-chromosome inactivation in mice by Tsix."Development. 128. 1275-1286 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hamatani, T.: "Epigenetic mark sequence of the H19 gene in human sperm."Biochim.Biophys.Acta. 1518. 137-144 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Aoki, A.: "Enzymatic properties of de novo-type mouse DNA (cytosine-5) methyltransferases."Nucl.Acids Res.. 29. 3506-3512 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Davies, K.: "Disruption of mesodermal enhancers for Igf2 in the minute mutant."Development. 129. 1657-1666 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sotomaru, Y.: "Upregulated expression of the imprinted genes H19 and Igf2r in mouse uniparental fetuses."J.Biol.Chem.. 277. 12474-12478 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ishihara, K.: "An evolutionarily conserved putative insulator element near the 3'boundary of the imprinted Igf2/H19 domain."Hum.Mol.Genet.. 11. 1627-1636 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kurosawa, K.: "Paternal UPD14 is responsible for a distinctive malformation complex."Am.J.Med.Genet.. 110. 268-272 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shirohzu, H.: "Three novel DNMT3B mutations in Japanese patients with ICF syndrome."Am.J.Med.Genet.. 112. 31-37 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sado, T.: "Effect of Tsix-disruption on Xsit expression in male ES cells."Cytogenet.Genome Res.. 99. 115-118 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ishida, C.: "Genomic organization and promoter analysis of the Dnmt3b gene."Gene. 310. 151-159 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hata, K.: "Genomic imprinting : mechanisms, significance and evolution."J.Mamm.Ova.Res.. 20. 64-68 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sado, T.: "De novo DNA methylation is dispensable for the initiation and propagation of X chromosome inactivation."Development. 131. 957-982 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kubota, T.: "ICF syndrome in a girl with DNA hypomethylation but without detectable DNMT38 mutation."Am.J.Med.Genet.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kaneda, M.: "Essential role for de novo DNA methyltransferase Dnmt3a in paternal and maternal imprinting."Nature. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Meguro, M.: "Large-scale evaluation of imprinting status in the Prader-Willi syndrome region : an imprinted direct repeat cluster resembling small nucleolar RNA genes."Hum.Mol.Genet.. 10. 383-394 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Meguro, M: "A novel maternally expressed gene, ATP10C, encodes a putative aminophospholipid translocase associated with Angelman syndrome."Nature Genet.. 28. 19-20 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohki, I.: "Solution structure of the methyl-CpG-binding domain of human MBD1 in complex with a methylated DNA."Cell. 105. 487-497 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takebayashi, S.: "5-aza-2'-deoxycytidine induces histone hyperacetylation of mouse centromeric heterochromatin by a mechanism independent of DNA demethylation."Biochem.Biophys.Res.Commun.. 288. 921-926 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kino, T.: "Identification of the cis-acting region in the NF2 gene promoter as a potential target for mutation and methylation-dependent silencing in schwannoma."Genes Cell. 6. 441-454 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takizawa, T.: "DNA methylation is a critical cell-intrinsic determinant of astrocyte differentiation in the fetal brain."Dev.Cell. 1. 749-758 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kudo, S.: "Functional analyses of McCP2 mutations associated with Rett syndrome using transient expression systems."Brain Dev.. 23 Suppl 1. S165-S173 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakao, M.: "Regulation of transcription and chromatin by methyl-CpG binding protein MBD1."Brain Dev.. 23 Suppl 1. S174-S176 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakao, M.: "Epigenetics : interactions of DNA methylation and chromatin."Gene. 278. 25-31 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kudo, S.: "Functional characterization of McCP2 mutations found in male patients with X-linked mental retardation."J.Med.Genet.. 39. 132-136 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kagotani, K.: "Visualization of transcription-dependent association of imprinted genes with the nuclear matrix."Exp.Cell Res.. 274. 189-196 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujita, N.: "MCAF mediates MBD1-dependent transcriptional repression."Mol.Cell.Biol.. 23. 2834-2843 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kudo, S.: "Heterogeneity in residual function of McCP2 carrying missense mutations in the methyl-CpG-binding domain."J.Med.Genet.. 40. 487-493 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujita, N.: "Methyl-CpG binding domain 1(MBD1) interacts with Suv39h1-HP1 heterochromatic complex for DNA methylation-based transcriptional repression."J.Biol.Chem.. 278. 24132-24138 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mizuno, S.: "Expression of DNA methyltransferases DNMT1,3A and 3B in normal hematopoiesis and in acute and chronic myelogenouse leukemia."Blood. 97. 1172-1179 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohno, M.: "Allele-specific detection of nascent transcripts by fluorescence in situ hybridization reveals temporal and culture-induced changes in Igf2 imprinting during pre-implantation mouse development."Genes.Cells. 6. 249-259 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sado, T.: "Regulation of imprinted X-chromosome inactivation in mice by Tsix."Development. 128. 1275-1286 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hamatani, T.: "Epigenetic mark sequence of the H19 gene in human sperm."Biochim.Biophys.Acta. 1518. 137-144 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Aoki, A.: "Enzymatic properties of de novo-type mouse DNA (cytosine-5) methyltransferases."Nucl.Acids Res.. 29. 3506-3512 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Davies, K.: "Disruption of mesodermal enhancers for Igf2 in the minute mutant."Development. 129. 1657-1666 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sotomaru, Y.: "Upregulated expression of the imprinted genes H19 and Igf2r in mouse uniparental fetuses."J.Biol.Chem.. 277. 12474-12478 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ishihara, k.: "An evolutionarily conserved putative insulator element near the 3' boundary of the imprinted Igf2/H19 domain."Human.Mol.Genet.. 11. 1627-1636 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kurosawa, k.: "Paternal UPD14 is responsible for a distinctive malformation complex."Am.J.Med.Genet.. 110. 268-272 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shirohzu, H.: "Three novel DNMT3B mutations in Japanese patients with ICF syndrome."Am.J.Med.Genet.. 112. 31-37 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sado, T: "Effect of Tsix-disruption on Xist expression in male ES cells."Cytogenet.Genome Res.. 99. 115-118 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ishida, C.: "Genomic organization and promoter analysis of the Dnmt3b gene."Gene. 310. 151-159 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hata, K.: "Genomic.imprinting : mechanisms, significance and evolution."J.Mamm.Ova Res.. 20. 64-68 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sado, T.: "De novo DNA methylation is dispensable for the initiation and propagation of X chromosome inactivation."Development. 131. 957-982 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kubota, T.: "ICF syndrome in a girl with DNA hypomethylation but without detectable DNMT3B mutation."Am.J.Med.Genet.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kaneda, M.: "Essential role for de novo DNA methyltransferase Dnmt3a in paternal and maternal imprinting."Nature. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Meguro, M.: "Large-scale evaluation of imprinting status in the Prader-Willi syndrome region : an imprinted direct repeat cluster resembling small nucleolar RNA genes."Hum.Mol.Genet.. 10. 383-394 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Meguro, M: "A novel maternally expressed gene, ATP10C, encodes a putative aminophospholipid translocase associated with Angelman syndrome."Nature Genet.. 28. 19-20 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohki, I.: "Solution structure of the methyl-CpG-binding domain of human MBD1 in complex with a methylated DNA."Cell. 105. 487-497 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takebayashi, S.: "5-aza-2'-deoxycytidine induces histone hyperacetylation of mouse centromeric heterochromatin by a mechanism independent of DNA demethylation."Biochem.Biophys.Res.Commun.. 288. 921-926 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kino, T.: "Identification of the cis-acting region in the NF2 gene promoter as a potential target for mutation and methylaton-dependent silencing in schwannoma."Genes Cell. 6. 441-454 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takizawa, T.: "DNA methylation is a critical cell-intrinsic determinant of astrocyte differentiation in the fetal brain."Dev.Cell. 1. 749-758 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kudo, S.: "Functional analyses of McCP2 mutations associated with Rett syndrome using transient expression systems."Brain Dev.. 23 Suppl 1. S165-S173 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakao, M.: "Regulation of transcription and chromatin by methyl-CpG binding protein MBD1."Brain Dev.. 23 Suppl 1. S174-S176 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakao, M.: "Epigenetics : interactions of DNA methylation and chromatin."Gene. 278. 25-31 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kudo, S.: "Functional characterization of McCP2 mutations found in male patients with X-linked mental retardation."J.Med.Genet.. 39. 132-136 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kagotani, k.: "Visualization of transcription-dependent association of imprinted genes with the nuclear matrix."ExCell Res.. 274. 189-196 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujita, N.: "MCAF mediates MBD1-dependent transcriptional repression."Mol.Cell.Biol.. 23. 2834-2843 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kudo, S.: "Heterogeneity in residual function of McCP2 carrying missense mutations in the methyl-CpG-binding domain."J.Med.Genet.. 40. 487-493 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujita, N.: "Methyl-CpG binding domain 1(MBD1) interacts with Suv39h1-HP1 heterochromatic complex for DNA methylation-based transcriptional repression."J.Biol.Chem.. 278. 24132-24138 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Watanabe, S.: "Methylated DNA-binding domain 1 and methylpurine-DNA glycosylase links transcriptional repression and DNA repair in chromatin."Proc.Natl.Acad.Sci.USA.. 100. 12859-12864 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ishida, C.: "Genomic organization and promoter analysis of the Dnmt3b gene."Gene. 310. 151-159 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hata, K.: "Genomic imprinting : mechanisms, significance and evolution."J.Mamm.Ova Res.. 20. 64-68 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sado, T.: "De novo DNA methylation is dispensable for the initiation and propagation of X chromosome inactivation."Development. 131. 957-982 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kubota, T.: "ICF syndrome in a girl with DNA hypomethylation but without detectable DNMT3B mutation."Am.J.Med.Genet.. in press.

    • Related Report
      2003 Annual Research Report
  • [Publications] Watanabe, S.: "Methylated DNA-binding domain 1 and methylpurine-DNA glycosylase links transcriptional repression and DNA repair in chromatin"Proc.Natl.Acad.Sci.USA.. 100. 12859-12864 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Fujita, N.: "MCAF mediates MBD1-dependent transcriptional repression"Mol.Cell.Biol.. 23. 2834-2843 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Fujita, N.: "Methyl-CpG binding domain 1 (MBD1) interacts with Suv39h1-HP1 heterochromatic complex for DNA methylation-based transcriptional repression"J.Biol.Chem.. 278. 24132-24138 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Matsuzaki, K.: "PML-nuclear bodies are involved in cellular serum response"Genes Cells. 8. 275-286 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Matsuzaki, K.: "Serum response factor is modulated by the SUMO-1 conjugation system"Biochem.Biophys.Res.Commun.. 306. 32-38 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kudo, S.: "Heterogeneity in residual function of MeCP2 carrying missense mutations in the methyl-CpG-binding domain"J.Med.Genet.. 40. 487-493 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Davies, K.: "Disruption of mesodermal enhancers for Igf2 in the minute mutant"Development. 129. 1657-1666 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sotomaru, Y.: "Upregulated expression of the imprinted genes H19 and Igf2r in mouse uniparental fetuses"J.Biol.Chem.. 277. 12474-12478 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ishihara, K.: "An evolutionarily conserved putative insulator element near the 3' boundary of the imprinted Igf2/H19 domain"Hum.Mol.Genet.. 11. 1627-1636 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kurosawa, K.: "Paternal UPD14 is responsible for a distinctive malformation complex"Am.J.Med.Genet.. 110. 268-272 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shirohzu, H.: "Three novel DNMT3B mutations in Japanese patients with ICF syndrome"Am.J.Med.Genet.. 112. 31-37 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sado, T.: "Effect of Tsix disruption on Xist expression in male ES cells"Cytogenet.Genome Res.. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Kudo, S.: "Functional characterization of MeCP2 mutations found in male patients with X-linked mental retardation"J.Med.Genet.. 39. 132-136 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kagotani, K.: "Visualization of transcription-dependent association of imprinted genes with the nuclear matrix"Exp.Cell Res.. 274. 189-196 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Fujita, N.: "MCAF mediates MBD1-dependent transcriptional repression"Mol.Cell.Biol.. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Matsuzaki, K.: "PML-nuclear bodies are involved in cellular serum response"Genes Cells. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Mizuno, S.: "Expression of DNA methyltransferases DNMTI, 3A and 3B in normal hematopoiesis and in acute and chronic myelogenous leukemia"Blood. 97. 1172-1179 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sado, T.: "Regulation of imprinted X-chromosome inactivation in mice by Tsix."Development. 128. 1275-1286 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hamatani, T.: "Epigenetic mark sequence of the H19 gene in human sperm"Biochim. Biophys. Acta. 1518. 137-144 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Aoki, A.: "Enzymatic properties of de novo-type mouse DNA (cytosine-5) methyltransferases"Nucl. Acids Res.. 29. 3506-3512 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takebayashi, S.: "5-Aza-2-deoxycytidine induces histone hyperacetylation of mouse centromeric het-erochromatin by a mechanism independent of DNA demethylation"Biochem. Biophys. Res. Commun.. 288. 921-926 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ohki, I.: "Solution structure of the methyl-CpG-binding domain of human MBD1 in complex with a methylated DNA"Cell. 105. 487-497 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takizawa, T.: "DNA methylation is a critical cell-intrinsic determinant of astrocyte differentiation in the fetal brain"Dev. Cell. 1. 749-758 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nakao, M.: "Epigenetics : interactions of DNA methylation and chromatin"Gene. 278. 25-31 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kino, T.: "Identification of the cis-acting region in the NF2 gene promoter as a potential target for mutation and methylation-dependent silencing in schwannoma"Genes Cells. 6. 441-454 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nakao, M.: "Regulation of transcription and chromatin by methyl-CpG binding protein MBD1."Brain Dev.. 23Suppl 1. S174-S176 (2001)

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi