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Nuclear transfer of mouse ES cells

Research Project

Project/Area Number 13308050
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory animal science
Research InstitutionKinki University

Principal Investigator

TSUNODA Yukio  Kinki University, College of Agriculture, Professor, 農学部, 教授 (80217364)

Co-Investigator(Kenkyū-buntansha) TANI Tetsuya  Kinki University, College of Agriculture, Research associate, 農学部, 助手 (70319763)
KATO Yoko  Kinki University, College of Agriculture, Associate Professor, 農学部, 助教授 (40278742)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥39,130,000 (Direct Cost: ¥30,100,000、Indirect Cost: ¥9,030,000)
Fiscal Year 2003: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Fiscal Year 2002: ¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000)
Fiscal Year 2001: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
KeywordsES cells / nuclear transfer / cloned mice
Research Abstract

Some of nuclear-transferred oocytes receiving mouse embryonic stem(ES) cells develop into fertile young. Although the developmental potential of nuclear-transferred oocytes that receive ES cells is high compared with those receive somatic cells, a large proportion die at various developmental stages and the rate of neonatal death is high. The present study examined the effect of 1)aggregation of nuclear-transferred oocytes to increase cell numbers, 2)genetic background of ES cells, 3)different nuclear transfer procedures, 4)cell cycle of ES cells, and 5)activation methods on cloning.
The following results were obtained. 1)The low potential of nuclear-transferred oocytes with ES cells to develop into young was not due to the lower cell number of nuclear transferred-embryos. 2)The cloning efficiency with hybrid ES cell lines was not superior to that of an inbred ES cell line. 3)The potential of nuclear-transferred oocytes to develop into blastocysts after fusion by Sendai virus was high compared with that after direct injection. 4)The developmental potential of oocytes receiving ES cells at the M phase was higher than that of oocytes receiving ES cells at the G1 phase. Different activation protocols did not affect the potential to develop into blastocysts. Since the developmental potential of nuclear-transferred oocytes to live pups was still low in all group(1 to 4%), further methodological studies to increase the viability of nuclear-transferred oocytes are required.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] T.Amano, Y.Kato, Y.Tsunoda: "The developmental potential of the inner cell mass of blastocysts that were derived from mouse ES cells using nuclear transfer technology"Cell Tissue Res. 307. 367-370 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] X.J.Yin, Y.Kato, Y.Tsunoda: "Effect of enucleation procedures and maturation conditions on the development of nuclear-transferred rabbit oocytes receiving male fibroblast cells"Reproduction. 124. 41-47 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Kawakami, T.Tani, X.j.Yin, Y.Kato, Y.Tsunoda: "Effect of oxygen tension on the developmental potential of parthenogenetic oocytes and nuclear-transferred porcine oocytes receiving fetal fibroblast cells"Journal of Reproduction Development. 48. 490-414 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] X.j.Yin, Y.Kato, Y.Tsunoda: "Effect of delayed enucleation on the developmental potential of nuclear-transferred oocytes receiving adult and fetal fibroblast cells"Zygote. 10. 217-222 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] A.Yabuuchi, Y.Kato, Y.Tsunoda: "Effects of aggregation of nuclear-transferred mouse embryos developed from enucleated eggs receiving ES cells on in vitro and in vivo development"Jounal of Reproduction Development. 48. 393-397 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Tani, Y.Kato, Y.Tsunoda: "Reprogramming of bovine somatic cell nuclei is not directly regulated by maturation promoting factor or mitogen-activated protein kinase activity"Biobgy of Reproduction. 69. 1890-1894 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Kawakami, T.Tani, A.Yabuuchi, T.Kobayshi, H.Murakami, T.Fujimura, Y.Kato, Y.Tsunoda: "Effect of demecolcine and nocodazole on the efficiency of chemically-assisted removal of chromosomes and the developmental potential of nuclear transferred porcine oocytes."Cloning and Stem Cells. 5. 379-387 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kato, Imabayashi, Mori, Tani, Taniguchi, Higashi, Matsumoto, Umezawa, Tsunoda: "Nuclear transfer of adult bone marrow mesenchymal stem cells : developmental totipotency of tissue-specific stem cells from an adult mammal"Blology of Reproduction. 70. 415-418 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] A.Yabuuchi, Y.Yasuda, Y.Kato, Y.Tsunoda: "Effect of nuclear transfer proccdures on ES cell cloning effeiciency in the mouse"Jounal of Reproduction and Development. (In press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Kobayashi, Y.Kato, Y.Tsunoda: "Effect of the timing of the first cleavage on the developmental potential of nuclear-transferred mouse oocytes receiving embryonic stem cells"Theriogenology. (In press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Kawano, Y.Kato, Y.Tsunoda: "Comparison of vitro development of porcine nuclear-transferred oocytes receiving fetal somatic cells by injection and fusion methods"Cloning and Stem Cells. (In press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Y.Tsunoda, Y.Kato: "Methods in Molecular Biology vo.1254Vol.2"Hmmana Press(In press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Amano, Y.Kato, Y.Tsunoda: "The developmental potential of the inner cell mass of blastocysts that were derived from mouse ES cells using nuclear transfer technology."Cell Tissue Res. 307. 367-370 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] X.J.Yin, Y.Kato, Y.Tsunoda: "Effect of enucleation procedures and maturation conditions on the development of nuclear-transferred rabbit oocytes receiving male fibroblast cells."Reproduction. 124. 41-47 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Kawakami, T.Tani, X.J.Yin, Y.Kato, Y.Tsunoda: "Effect of oxygen tension on the developmental potential of parthenogenetic oocytes and nuclear-transferred porcine oocytes receiving fetal fibroblast cells."Journal of Reproduction Development. 48. 414-490 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] X.J.Yin, Y.Kato, Y.Tsunoda: "Effect of delayed enucleation on the developmental potential of nuclear-transferred oocytes receiving adult and fetal fibroblast cells."Zygote. 10. 217-222 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] A.Yabuuchi, Y.Kato, Y.Tsunoda: "Effects of aggregation of nuclear-transferred mouse embryos developed from enucleated eggs receiving ES cells on in vitro and in vivo development."Journal of Reproduction Development. 48. 393-397 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Tani, Y.Kato, Y.Tsunoda: "Reprogramming of bovine somatic cell nuclei is not directly regulated by maturation promoting factor or mitogen-activated protein kinase activity."Biology of Reproduction. 69. 1890-1894 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Kawakami, T.Tani, A.Yabuuchi, T.Kobayashi, H.Murakami, T.Fujimura, Y.Kato, Y.Tsunoda: "Effect of demecolcine and nocodazole on the efficiency of chemically-assisted removal of chromosomes and the developmental potential of nuclear transferred porcine oocytes."Cloning and Stem Cells. 5. 379-387 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Y.Kato, Imabayashi, Mori, Tani, Taniguchi, Higashi, Matsumoto, Umezawa, Tsunoda: "Nuclear transfer of adult bone marrow mesenchymal stem cells : developmental totipotency of tissue-specific stem cells from an adult mammal."Biology of Reproduction. 70. 415-418 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] A.Yabuuchi, Y.Yasuda, Y.Kato, Y.Tsunoda: "Effect of nuclear transfer procedures on ES cell cloning effeiciency in the mouse."Journal of Reproduction Development. (In press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Kobayashi, Y.Kato, Y.Tsunoda: "Effect of the timing of the first cleavage on the developmental potential of nuclear-transferred mouse oocytes receiving embryonic stem cells."Theriogenology. (In press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Kawano, Y.Kato, Y.Tsunoda: "Comparison of vitro development of porcine nuclear-transferred oocytes receiving fetal somatic cells by injection and fusion methods."Cloning and Stem Cells. (In press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Tani, Y.Kato, Y.Tsunoda: "Reprogramming of bovine somatic cell nuclei is not directly regulated by maturation promoting factor or mitogen-activated protein kinase activity"Biology of Reproduction. 69. 1890-1894 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Kawakami, Tani, T, Yabuuchi, A, Kobayashi, T, Murakami, H, Fujimura, Y, Kato, Y.Tsunoda: "Effect of demecolcine and nocodazole on efficiency of chemically assisted removal of chromosomes and the developmental potential of nuclear transferred porcine oocytes"Cloning and Stem Cells. 5. 379-387 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kato, Imabayashi, Mori, Tani, Taniguchi, Higashi, Matsunoto, Umezawa, Tsunoda: "Nuclear transfer of adult bone marrow mesenchymal stem cells : developmental totipotency of tissue-specific stem cells from adult mammal"Biology of Reproduction. 70. 415-418 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] A.Yabuuchi, Y.Yasuda, Y.Kato, Y.Tsunoda: "Effect of nuclear transfer procedures on ES cell cloning efficiency in the mouse"J.Reprod.Develop.. (In press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Kobayashi, Y.Kato, Y.Tsunoda: "Effect of the timing of the first cleavage on the developmental potential of nuclear-transferred mouse oocytes receiving embryonic stem cells"Theriogenology. (In press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Kawano, Y.Kato, Y.Tsunoda: "Comparison of in vitro development of porcine nuclear-transferred oocytes receiving fetal somatic cells by injection and fusion methods"Cloning and Stem Cells. (In press).

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Amano, Y.Kato, Y.Tsunoda: "The developmental potential of inner cell mass of blastocysts that were derived from mouse ES cell using transfer technology"Cell Tissue Res. 307. 367-370 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] X.J.Yin, Y.Kato, Y.Tsunoda: "Effect of enucleation procedures and maturation conditions on the development of nuclear transferred rabbit oocytes receiving male fibroblast cells"Reproduction. 124. 41-47 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Kawakami, T.Tani, X.J.Yin, Y.Kato, Y.Tsunoda: "Effect of oxygen tension on the developmental potential of parthenogenetic oocytes and nuclear transferred norcine oocytes receiving fatal fibroblast cells"Journal of Reproduction and Development. 48. 409-414 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] X.J.Yin, Y.Kato, Y.Tsunoda: "Effect of delayed enucleation on the developmental potential of nuclear transferred oocytes receiving adult and fetal fibroblast cells"Zygote. 10. 217-222 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] A.Yabuuchi, Y.Kato, Y.Tsunoda: "Effects of aggregation of nuclear transferred mouse embryos developed from enucleated eggs receiving ES cells on in vitro and in vivo development"Journal of Reproduction and Development. 48. 393-397 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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