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Development of international standard diagnostic method for equine babesiosis

Research Project

Project/Area Number 13356007
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Applied veterinary science
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

IGARASHI Ikuo  Obihiro University of Agriculture and Veterinary Medicine, National Research Center for Protozoan Diseases, Professor, 原虫病研究センター, 教授 (80159582)

Co-Investigator(Kenkyū-buntansha) FUJISAKI Kozo  Obihiro University of Agriculture and Veterinary Medicine, National Research Center for Protozoan Diseases, Professor, 原虫病研究センター, 教授 (00292095)
XUAN Xuenan  Obihiro University of Agriculture and Veterinary Medicine, National Research Center for Protozoan Diseases, Associate Professor, 原虫病研究センター, 助教授 (10292096)
YOKOYAMA Naoaki  Obihiro University of Agriculture and Veterinary Medicine, National Research Center for Protozoan Diseases, Associate Professor, 原虫病研究センター, 助教授 (80301802)
INOUE Noboru  Obihiro University of Agriculture and Veterinary Medicine, National Research Center for Protozoan Diseases, Associate Professor, 原虫病研究センター, 助教授 (10271751)
IKADAI Hiromi  Kitasato University, Faculty of Veterinary Medicine and animal sciences., Associate Professor, 獣医畜産学部, 助手 (80327460)
Project Period (FY) 2001 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥44,200,000 (Direct Cost: ¥34,000,000、Indirect Cost: ¥10,200,000)
Fiscal Year 2004: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2003: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2002: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2001: ¥21,060,000 (Direct Cost: ¥16,200,000、Indirect Cost: ¥4,860,000)
KeywordsEquine babesiosis / Babesia equi / Babesia caballi / Serological diagnosis / Recombinant antigen / ELISA / Immunochromatographic test / International standard method / 血清診断 / 金コロイド粒子 / ディップスティック法 / 国際標準診断法 / ウマバベシア / ラテックス凝集反応 / 国際標準
Research Abstract

Equine babesiosis, caused by Babesia equi and Babesia caballi, is characterized by fever, anemia, icterus, hepatosplenomegaly, lethargy, and, in some cases, leading to great economic losses in the horse industry. The infected horses often remain carriers of the parasites for a long period and are known to act as sources for subsequent infections to other horses via tick vectors. Therefore, the development of a high-quality system has been required for the serological diagnosis of babesial infection. In the present study, development of international standard diagnostic methods with high specificity and sensitivity for equine babesiosis was examined by gene cloning of Babesia DNA with immune sera, production of recombinant antigens, evaluation of ELISA and imunochromatographic test using those antigens. Novel genes, Be82, Be158 and Bc134, were cloned from B.equi and B.caballi, respectively. ELISA using these recombinant antigens showed the high specificity and sensitivity for the detect … More ion of antibodies against both babesial infections. Productions of recombinant antigens in Escherichia coli, EMA-2 and BC48, were also improved by the construction of truncated genes or modification of culture or induction factors. ELISA using these truncated EMA-2 and BC48 showed high specificity and sensitivity for the detection of antibodies. An immunochromotographic test for simultaneous detection of both B.caballi and B.equi specific antibodies was developed. Evaluation in the detection of sera from uninfected horses and experimentally infected horses showed high sensitivity and specificity as shown in ELISA. A differential single-round and multiplex polymerase chain reaction (PCR) method was also developed for the simultaneous detection of B.caballi and B.equi, by targeting 18S ribosomal RNA genes. In conclusion, EMA-2 for B.equi and BC48 for B.caballi were most excellent as antigens for the diagnosis. ELISA and immunochromatographic test using both antigens have shown to have high specificity and sensitivity for the detection of antibodies. The worldwide experimental comparison and evaluation of these serological tests and genetic analysis will would be feasible tests for internationally recognized diagnosis for equine babesiosis in the world. Less

Report

(5 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (70 results)

All 2005 2004 2003 2002 2001 1999 Other

All Journal Article (50 results) Book (1 results) Patent(Industrial Property Rights) (2 results) Publications (17 results)

  • [Journal Article] Epidemiological study of equine piroplasmosis in Mongolia.2005

    • Author(s)
      Boldbaater D, Xuan X, Battsetseg B, Igarashi I, Bathur B, Batsukh Z, Bayambaa B, Fujisaki K.
    • Journal Title

      Vet.Parasitol. 127

      Pages: 35-38

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Host serum modifies the drug susceptibility of Babesia bovis in vitro2005

    • Author(s)
      Bork S, Okamura M, Matsuo T, Kumar S, Yokoyama N, Igarashi I.
    • Journal Title

      Parasitology 130

      Pages: 489-492

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Development of a single-round and multiplex PCR method for the simultaneous detection of Babesia caballi and Babesia equi in horse blood.2005

    • Author(s)
      Alhassan A, Pumidonming W, Okamura M, Hirata H, Battsetseg B, Fujisaki K, Yokoyama N, Igarashi
    • Journal Title

      Vet.Parasitol. 129

      Pages: 43-49

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Epidemiological study of equine piroplasmosis in Mongolia.2005

    • Author(s)
      Boldbaatar D, Xuan X, Battsetseg B, Igarashi I, Battur B, Batsukh Z, Bayambaa B, Fujisaki K.
    • Journal Title

      Vet Parasitol. 127

      Pages: 35-38

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cloning of a novel Babesia equi gene encoding a 158-kilodalton protein useful for serological diagnosis.2005

    • Author(s)
      Hirata H, Yokoyama N, Xuan X, Fujisaki K, Suzuki N, Igarashi I.
    • Journal Title

      Clin Diagn Lab Immunol. 12

      Pages: 334-338

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Host serum modifies the drug susceptibility of Babesia bovis in vitro.2005

    • Author(s)
      Bork S, Okamura M, Matsuo T, Kumar S, Yokoyama N, Igarashi I.
    • Journal Title

      Parasitology (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Development of a single-round and multiplex PCR method for the simultaneous detection of Babesia caballi and Babesia equi in horse blood.2005

    • Author(s)
      Alhassan A, Pumidonming W, Okamura M, Hirata H, Battsetseg B, Fujisaki K, Yokoyama N, Igarashi I.
    • Journal Title

      Vet Parasitol. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Epidemiological study of equine piroplasmosis in Mongolia.2005

    • Author(s)
      Boldbaatar D, Xuan X, Battsetseg B, Igarashi I, Battur B, Batsukh Z, Bayambaa B, Fujisaki K.
    • Journal Title

      Vet.Parasitol. 127

      Pages: 35-38

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Development of a single-round and multiplex PCR method for the simultaneous detection of Babesia caballi and Babesia equi in horse blood.2005

    • Author(s)
      Alhassan A, Pumidonming W, Okamura M, Hirata H, Battsetseg B, Fujisaki K, Yokoyama N, Igarashi
    • Journal Title

      Vet.Parasitol. 129・1-2

      Pages: 43-49

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Host serum modifies the drug susceptibility of Babesia bovis in vitro2005

    • Author(s)
      Bork S, Okamura M, Matsuo T, Kumar S, Yokoyama N, Igarashi I.
    • Journal Title

      Parasitology 129(in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Identification of Babesia bovis L-lactate dehydrogenase as a potential chemotherapeutical target against bovine babesiosis.2004

    • Author(s)
      Bork S, Okamura M, Boonchit S, Hirata H, Yokoyama N, Igarashi I.
    • Journal Title

      Mol.Biochem.Parasitol. 136

      Pages: 165-172

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Serodiagnosis of Babesia gibsoni infection in dogs by an improved enzyme-linked immunosorbent assay with recombinant truncated P50.2004

    • Author(s)
      Verdida RA, Hara OA, Xuan X, Fukumoto S, Igarashi I, Zang S, Dong J, Inokuma H, Kabeya H, et al.
    • Journal Title

      J.Vet.Med.Sci. 66

      Pages: 1517-1521

    • NAID

      110003963643

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cloning of a novel Babesia equi gene encoding a 158-kilodalton protein useful for serological diagnosis.2004

    • Author(s)
      Hirata H, Yokoyama N, Xuan X, Fujisaki K, Suzuki N, Igarashi I.
    • Journal Title

      Clin.Diagn.Lab.Immunol. 12

      Pages: 334-338

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Growth-inhibitory effect of heparin on Babesia parasites.2004

    • Author(s)
      Bork S, Yokoyama N, Ikehara Y, Kumar S, Sugimoto C, Igarashi I.
    • Journal Title

      Antimicrob Agents Chemother. 48

      Pages: 236-341

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular cloning of a Babesia caballi gene encoding the 134-kilodalton protein and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay.2004

    • Author(s)
      Tamaki Y, Hirata H, Takabatake N, Bork S, Yokoyama N, Xuan X, Fujisaki K, Igarashi I.
    • Journal Title

      Clin Diagn Lab Immunol. 11

      Pages: 211-215

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Development of an immunochromatographic test with recombinant EMA-2 for the rapid detection of antibodies against Babesia equi in horses.2004

    • Author(s)
      Huang X, Xuan X, Xu L, Zhang S, Yokoyama N, Suzuki N, Igarashi I.
    • Journal Title

      J Clin Microbiol. 42

      Pages: 359-361

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of Babesia equi EMA-1 and EMA-2 during merozoite developmental stages in erythrocyte and their interaction with erythrocytic membrane skeleton.2004

    • Author(s)
      Kumar S, Yokoyama N, Kim JY, Huang X, Inoue N, Xuan X, Igarashi I, Sugimoto C.
    • Journal Title

      Mol Biochem Parasitol. 133

      Pages: 221-227

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Inhibitory effect of antiserum to surface antigen P50 of Babesia gibsoni on growth of parasites in severe combined immunodeficiency mice given canine red blood cells.2004

    • Author(s)
      Fukumoto S, Xuan X, Takabatake N, Igarashi I, Sugimoto C, Fujisaki K, Nagasawa H, Mikami T, Suzuki H.
    • Journal Title

      Infect Immun. 72

      Pages: 1795-1798

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Improved enzyme-linked immunosorbent assay using C-terminal truncated recombinant antigens of Babesia bovis rhoptry-associated protein-1 for detection of specific antibodies.2004

    • Author(s)
      Boonchit S, Xuan X, Yokoyama N, Goff WL, Waghela SD, Wagner G, Igarashi I.
    • Journal Title

      J Clin Microbiol. 42

      Pages: 1601-1604

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Serodiagnosis of canine Babesia gibsoni infection by enzyme-linked immunosorbent assay with recombinant P50 expressed in Escherichia coli.2004

    • Author(s)
      Fukumoto S, Sekine Y, Xuan X, Igarashi I, Sugimoto C, Nagasawa H, Fujisaki K, Mikami T, Suzuki H.
    • Journal Title

      J Parasitol. 90

      Pages: 387-391

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular evidence of infections with Babesia gibsoni parasites in Japan and evaluation of the diagnostic potential of a loop-mediated isothermal amplification method.2004

    • Author(s)
      Ikadai H, Tanaka H, Shibahara N, Matsuu A, Uechi M, Itoh N, Oshiro S, Kudo N, Igarashi I, Oyamada T.
    • Journal Title

      J Clin Microbiol. 42

      Pages: 2465-2469

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification of Babesia bovis L-lactate dehydrogenase as a potential chemotherapeutical target against bovine babesiosis.2004

    • Author(s)
      Bork S, Okamura M, Boonchit S, Hirata H, Yokoyama N, Igarashi I.
    • Journal Title

      Mol Biochem Parasitol. 136

      Pages: 165-172

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Serodiagnosis of Babesia gibsoni infection in dogs by an improved enzyme-linked immunosorbent assay with recombinant truncated P50.2004

    • Author(s)
      Verdida RA, Hara OA, Xuan X, Fukumoto S, Igarashi I, Zhang S, Dong J, Inokuma H, Kabeya H, Sato Y, Moritomo T, Maruyama S, Claveria F, Nagasawa H.
    • Journal Title

      J Vet Med Sci. 2004 66

      Pages: 1517-1521

    • NAID

      110003963643

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Serodiagnosis of Babesia gibsoni infection in dogs by an improved enzyme-linked immunosorbent assay with recombinant truncated P50.2004

    • Author(s)
      Verdida RA, Hara OA, Xuan X, Fukumoto S, Igarashi I, Zhang S, Dong J, Inokuma H, Kabeya H, et al.
    • Journal Title

      J.Vet.Med.Sci. 66

      Pages: 1517-1521

    • NAID

      110003963643

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Roles of the Maltese cross form in the development of parasitemia and protection against Babesia microti infection in mice.2003

    • Author(s)
      Yokoyama N, Bork S, Nishisaka M, Hirata H, Matsuo T, Inoue N, Xuan X, Suzuki H, Sugimoto C, Igarashi I.
    • Journal Title

      Infect Immun.2003 Jan 71

      Pages: 411-417

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Growth-inhibitory effects of artesunate, pyrimethamine, and pamaquine against Babesia equi and Babesia caballi in in vitro cultures.2003

    • Author(s)
      Nagai A, Yokoyama N, Matsuo T, Bork S, Hirata H, Xuan X, Zhu Y, Claveria FG, Fujisaki K, Igarashi I.
    • Journal Title

      Antimicrob Agents Chemother. 47

      Pages: 800-803

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification of a specific antigenic region of the P82 protein of Babesia equi and its potential use in serodiagnosis.2003

    • Author(s)
      Hirata H, Xuan X, Yokoyama N, Nishikawa Y, Fujisaki K, Suzuki N, Igarashi I.
    • Journal Title

      J Clin Microbiol. 41

      Pages: 547-551

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] High-level expression and purification of a truncated merozoite antigen-2 of Babesia equi in Escherichia coli and its potential for immunodiagnosis.2003

    • Author(s)
      Huang X, Xuan X, Yokoyama N, Xu L, Suzuki H, Sugimoto C, Nagasawa H, Fujisaki K, Igarashi I.
    • Journal Title

      J Clin Microbiol. 41

      Pages: 1147-1151

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Growth inhibitory effect of triclosan on equine and bovine Babesia parasites.2003

    • Author(s)
      Bork S, Yokoyama N, Matsuo T, Claveria FG, Fujisaki K, Igarashi I.
    • Journal Title

      Am J Trop Med Hyg. 68

      Pages: 334-340

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Clotrimazole, ketoconazole, and clodinafop-propargyl as potent growth inhibitors of equine Babesia parasites during in vitro culture.2003

    • Author(s)
      Bork S, Yokoyama N, Matsuo T, Claveria FG, Fujisaki K, Igarashi I.
    • Journal Title

      J Parasitol. 89

      Pages: 604-606

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] High-level expression of truncated surface antigen P50 of Babesia gibsoni in insect cells by baculovirus and evaluation of its immunogenicity and antigenicity.2003

    • Author(s)
      Fukumoto S, Xuan X, Kadota K, Igarashi I, Sugimoto C, Fujisaki K, Nagasawa H, Mikami T, Suzuki H.
    • Journal Title

      Clin Diagn Lab Immunol. 10

      Pages: 596-601

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Seroepidemiologic studies on Babesia equi and Babesia caballi infections in horses in Jilin province of China.2003

    • Author(s)
      Xu Y, Zhang S, Huang X, Bayin C, Xuan X, Igarashi I, Fujisaki K, Kabeya H, Maruyama S, Mikami T.
    • Journal Title

      J Vet Med Sci. 65

      Pages: 1015-1017

    • NAID

      110003886302

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular characterization of a gene encoding a 29-kDa cytoplasmic protein of Babesia gibsoni and evaluation of its diagnostic potentiality.2003

    • Author(s)
      Fukumoto S, Xuan X, Inoue N, Igarashi I, Sugimoto C, Fujisaki K, Nagasawa H, Mikami T, Suzuki H.
    • Journal Title

      Mol Biochem Parasitol. 131

      Pages: 129-136

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Clotrimazole, ketoconazole, and clodinafop-propargyl inhibit the in vitro growth of Babesia bigemina and Babesia bovis (Phylum Apicomplexa).2003

    • Author(s)
      Bork S, Yokoyama N, Matsuo T, Claveria FG, Fujisaki K, Igarashi I.
    • Journal Title

      Parasitology. 127

      Pages: 311-315

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cloning of a truncated Babesia equi gene encoding an 82-kilodalton protein and its potential use in an enzyme-linked immunosorbent assay.2002

    • Author(s)
      Hirata H, Ikadai H, Yokoyama N, Xuan X, Fujisaki K, Suzuki N, Mikami T, Igarashi I.
    • Journal Title

      J Clin Microbiol. 40

      Pages: 1470-1474

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Seroepidemiologic studies on Babesia caballi and Babesia equi infections in Japan.2002

    • Author(s)
      Ikadai H, Nagai A, Xuan X, Igarashi I, Tsugihiko K, Tsuji N, Oyamada T, Suzuki N, Fujisaki K.
    • Journal Title

      J Vet Med Sci. 64

      Pages: 325-328

    • NAID

      110003920850

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Detection of natural infection of Boophilus microplus with Babesia equi and Babesia caballi in Brazilian horses using nested polymerase chain reaction.2002

    • Author(s)
      Battsetseg B ; Lucero S, Xuan X, Claveria FG, Inoue N, Alhassan A, Kanno T, Igarashi I, Nagasawa H, Mikami T, Fujisaki K.
    • Journal Title

      Vet Parasitol. 107

      Pages: 351-357

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Diagnosis of equine piroplasmosis in Xinjiang province of China by the enzyme-linked immunosorbent assays using recombinant antigens.2002

    • Author(s)
      Xuan X, Chahan B, Huang X, Yokoyama N, Makala LH, Igarashi I, Fujisaki K, Maruyama S, Sakai T, Mikami T.
    • Journal Title

      Vet Parasitol. 108

      Pages: 179-182

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Detection of equine Babesia spp.gene fragments in Dermacentor nuttalli Olenev 1929 infesting mongolian horses, and their amplification in egg and larval progenies.2002

    • Author(s)
      Battsetseg B, Lucero S, Xuan X, Claveria F, Byambaa B, Battur B, Boldbaatar D, Batsukh Z, Khaliunaa T, Battsetseg G, Igarashi I, Nagasawa H, Fujisaki K.
    • Journal Title

      J Vet Med Sci. 64

      Pages: 727-730

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cellular localization of Babesia bovis merozoite rhoptry-associated protein 1 and its erythrocyte-binding activity.2002

    • Author(s)
      Yokoyama N, Suthisak B, Hirata H, Matsuo T, Inoue N, Sugimoto C, Igarashi I.
    • Journal Title

      Infect Immun. 70

      Pages: 5822-5826

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Evaluation of an enzyme-linked immunosorbent assay with recombinant rhoptry-associated protein 1 antigen against Babesia bovis for the detection of specific antibodies in cattle.2002

    • Author(s)
      Boonchit S, Xuan X, Yokoyama N, Goff WL, Wagner G, Igarashi I.
    • Journal Title

      J Clin Microbiol. 40

      Pages: 3771-3775

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of Babesia equi merozoite antigen 1 in insect cells by recombinant baculovirus and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay.2001

    • Author(s)
      Xuan X, Larsen A, Ikadai H, Tanaka T, Igarashi I, Nagasawa H, Fujisaki K, Toyoda Y, Suzuki N, Mikami T.
    • Journal Title

      J Clin Microbiol. 39

      Pages: 705-709

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Detection of Babesia caballi and Babesia equi in Dermacentor nuttalli adult ticks.2001

    • Author(s)
      Battsetseg B, Xuan X, Ikadai H, Bautista JL, Byambaa B, Boldbaatar D, Battur B, Battsetseg G, Batsukh Z, Igarashi I, Nagasawa H, Mikami T, Fujisaki K.
    • Journal Title

      Int J Parasitol. 31

      Pages: 384-386

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Detection of antibodies to Babesia equi in horses by a latex agglutination test using recombinant EMA-1.2001

    • Author(s)
      Xuan X, Igarashi I, Tanaka T, Fukumoto S, Nagasawa H, Fujisaki K, Mikami T.
    • Journal Title

      Clin Diagn Lab Immunol. 8

      Pages: 645-646

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification and expression of a 50-kilodalton surface antigen of Babesia gibsoni and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay.2001

    • Author(s)
      Fukumoto S, Xuan X, Nishikawa Y, Inoue N, Igarashi I, Nagasawa H, Fujisaki K, Mikami T.
    • Journal Title

      J Clin Microbiol. 39

      Pages: 2603-2609

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Development of a polymerase chain reaction method for diagnosing Babesia gibsoni infection in dogs.2001

    • Author(s)
      Fukumoto S, Xuan X, Shigeno S, Kimbita E, Igarashi I, Nagasawa H, Fujisaki K, Mikami T.
    • Journal Title

      J Vet Med Sci. 63

      Pages: 977-981

    • NAID

      110003920731

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Diagnosis of equine piroplasmosis in Brazil by serodiagnostic methods with recombinant antigens.2001

    • Author(s)
      Xuan X, Nagai A, Battsetseg B, Fukumoto S, Makala LH, Inoue N, Igarashi I, Mikami T, Fujisaki K.
    • Journal Title

      J Vet Med Sci. 63

      Pages: 1159-1160

    • NAID

      110003920767

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterisation of the gene encoding a protective antigen from Babesia microti identified it as eta subunit of chaperon in containing T-complex protein 1.2001

    • Author(s)
      Nishisaka M, Yokoyama N, Xuan X, Inoue N, Nagasawa H, Fujisaki K, Mikami T, Igarashi I.
    • Journal Title

      Int J Parasitol. 31

      Pages: 1673-1679

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular evidence of Babesia caballi (Nuttall and Strickland, 1910) parasite transmission from experimentally-infected SLID mice to the ixodid tick, Haemaphysalis longicornis (Neuman,1901).2001

    • Author(s)
      Rodriguez Bautista JL, Ikadai H, You M, Battsetseg B, Igarashi I, Nagasawa H, Fujisaki K.
    • Journal Title

      Vet Parasitol. 102

      Pages: 185-191

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Analysis of a growth-promoting factor for Babesia caballi cultivation.2001

    • Author(s)
      Ikadai H, Martin MD, Nagasawa H, Fujisaki K, Suzuki N, Mikami T, Kudo N, Oyamada T, Igarashi I.
    • Journal Title

      J Parasitol. 87

      Pages: 1484-1486

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] トキソプラズマ症、バベシア症、人獣共通感染症(木村哲、喜田宏編)2004

    • Author(s)
      五十嵐郁男
    • Publisher
      医薬ジャーナル社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Patent(Industrial Property Rights)] ウマバベシア感染用診断キット2005

    • Inventor(s)
      五十嵐 郁男, 黄 暁黄, 玄 学南
    • Industrial Property Rights Holder
      帯広畜産大学
    • Industrial Property Number
      2005-008062
    • Filing Date
      2005-01-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Patent(Industrial Property Rights)] Gene encoding merozoiac protein of Babesia caballi, recombinat protein obtained therefrom and utilization thereof1999

    • Inventor(s)
      Hiromi Ikadai, Ikuo Igarashi, et al.
    • Industrial Property Rights Holder
      Takesi Mikami, Chemo-Sero Therapeutic Research Instisute
    • Filing Date
      1999-08-13
    • Acquisition Date
      2005-02-15
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Bork S, Yokoyama N, Matsuo T, Claveria FG, Fujisaki K, Igarashi I.: "Clotrimazole, ketoconazole, and clodinafop-propargyl as potent growth inhibitors of equine Babesia parasites during in vitro culture"J.Parasitol.. 89(3). 604-606 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Huang X, Xuan X, Xu L, Zhang S, Yokoyama N, Suzuki N, Igarashi I.: "Development of an immunochromatographic test with recombinant EMA-2 for the rapid detection of antibodies against Babesia equi in horses"J Clin Microbiol. 42(1). 359-361 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Huang X, Xuan X, Hirata H, Yokoyama N, Xu L, Suzuki N, Igarashi I.: "Rapid immunochromatographic test using recombinant SAG2 for detection of antibodies against Toxoplasma, gondii in cats"J Clin Microbiol. 42(2). 129-136 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tamaki Y, Hirata H, Takabatake N, Bork S, Yokoyama N, Xuan X, Fujisaki K et al.: "Molecular cloning of a Babesia caballi gene encoding the 134-kilodalton protein and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay"Clin Diagn Lab Immunol. 11(1). 211-215 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Bork S, Yokoyama N, Ikehara Y, Kumar S, Sugimoto C. Igarashi I.: "Growth-inhibitory effect of heparin on Babesia parasites"Antimicrob Agents Chemother.. 48(1). 236-241 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Xu Y, Zhang S, Huang X, Bayin C, Xuan X, Igarashi I, et al.: "Seroepidemiologic studies on Babesia equi and Babesia caballi infections in horses in Jilin province of China"J.Vet.Med.Sci.. 65(19). 1015-1017 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Huang X, Xuan X, Yokoyama N, Xu L, Suzuki H, Sugimoto C, Nagasawa H, et al.: "High-Level Expression and Purification of a Truncated Merozoite Antigen-2 of Babesia equi in Escherichia coli and Its Potential for Immunodiagnosis"J Clin Microbiol.. 41(3). 1147-1151 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hirata H, Xuan X, Yokoyama N, Nishikawa Y, Fujisaki K, Suzuki N, Igarashi I.: "Identification of a Specific Antigenic Region of the p82 Protein of Babesia equi and Its Potential Use in Serodiagnosis"J Clin Microbiol.. 41(2). 547-551 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nagai A, Yokoyama N, Matsuo T, Bork S, Hirata H, Xuan X, Zhu Y, Claveria FG, et al.: "Growth-Inhibitory Effects of Artesunate, Pyrimethamine, and Pamaquine against Babesia equi and Babesia caballi in In Vitro Cultures"Antimicrob Agents Chemother.. 47(2). 800-803 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yokoyama N, Bork S, Nishisaka M, Hirata H, Matsuo T, Inoue N, Xuan et al.: "Roles of the Maltese cross form in the development of parasitemia and protection against Babesia microti infection in mice"Infect Immun.. 71(1). 411-417 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Boonchit S, Xuan X, Yokoyama N, Goff WL, Wagner G, Igarashi I.: "Evaluation of an enzyme-linked immunosorbent assay with recombinant rhoptry-associated protein 1 antigen against Babesia bovis for the detection of specific antibodies in cattle"J.Clin.Micorbiol.. 40(10). 3771-3775 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hirata, H., Ikadai, H., Yokoyama, N., Xuan, X., Fujisaki, K., et al.: "Cloning of a truncated Babesia equi gene encoding a 82-kilodalton protein and its potential use in an enzyme-linked immunosorbent assay"J.Clin.Micorbiol.. 40(4). 1470-1474 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hirata, H., Ikadai, H., Yokoyama, N., Xuan, X., Fujisaki, K., et al.: "Cloning of a truncated Babesia equi gene encoding a 82-kilodalton protein and its potential use in an enzyme-linked immunosorbent assay"J. Clin. Micorbiol. 40(4). in press (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ikadai, I., Martin MD, Nagasawa, N., et al.: "Analysisi sof growth-promoting factor for Babesia caballi cultivation"J. Parasitol. 87(6). 1484-1486 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nishisaka M, Yokoyama N, Xuan X, Inoue N, Nagasawa H, Fujisaki K, Mikami T, Igarashi I.: "Characterisation of the gene encoding a protective antigen from Babesia microti identified it as eta subunit of chaperonin containing T-complex protein 1"Int.J. Parasitol.. 31(14). 1673-1679 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Battsetseg, B., Xuan, X., Jose, R., Byambaa, B., Boldbaatar D., et al.: "Diagnosis of Babesia caballi and Babesia equi infections in Dermacentor nuttalli adult"Int.J. Parasitol.. 31(4). 384-386 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Xuan, X., Larsen, A., Ikadai, H., Tanaka, T., Igarashi, I. Et al.: "Expression of Babesia equi merozoite antigen-1 in insect cells by recombinant baculovirus and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay"J. Clin. Micorbiol. 39(2). 705-709 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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