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Development of genotyping method for TT virus (TTV) and its application to elucidation of pathogenesis.

Research Project

Project/Area Number 13357003
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Virology
Research InstitutionJICHI MEDICAL SCHOOL

Principal Investigator

OKAMOTO Hiroaki (2003)  JICHI MEDICAL SCHOOL, SCHOOL OF MEDICINE, PROFESSOR, 医学部, 教授 (30177092)

真弓 忠 (2001-2002)  自治医科大学, 医学部, 教授 (00049016)

Co-Investigator(Kenkyū-buntansha) NISHIZAWA Tsutomu  JICHI MEDICAL SCHOOL, SCHOOL OF MEDICINE, ASSOCIATE PROFESSOR, 医学部, 講師 (30306112)
岡本 宏明  自治医科大学, 医学部, 助教授 (30177092)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥26,910,000 (Direct Cost: ¥20,700,000、Indirect Cost: ¥6,210,000)
Fiscal Year 2003: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2002: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2001: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
KeywordsTTV / Tprque genotype virus / phylogenetic tree / PCR / Genetic group / selective PCR / selective PCR / TTウイルス(TTV) / Neighbor-joining法 / TTウイルス
Research Abstract

In 1997, we isolated a novel DNA virus from the serum of a patient with posttransfusion acute hepatitis of unknown etiology and named it TT virus (TTV) after the initials (T.T.) of the index patient. Recently, taxonomic nomenclature for TTV has been proposed by the TTV study group of the International Committee on Taxonomy of Viruses (ICTV) : head investigator of this project (H.O.) was a member of the study group. It was designated as Torque Teno virus (TTV): this name was derived from "Torque" which means the necklace, and "Tenuis" means thin, and relates to circular, single-stranded nature of its DNA genome. TTV was classified into a novel floating genus, the Anellovirus genus, which has not yet been assigned to a family. The name of the genus, Anellovirus, was derived from "anello," which means the ring, and relates to the circular nature of the DNA genome. TTV has a wide range of sequence divergence for a DNA virus, and approximately 40 genotypes have been identified. In the present study, we classified them further into five distantly related genetic groups (Groups 1-5), and developed a selective PCR method using oligonulceotide primers specific for TTVs of each of the five genetic groups to classify TTVs into the five genetic groups. Using this PCR method, we examined the prevalence of five genetic groups of TTV in relation to age. TTVs of group 4 were prevalent in early childhood, and detected most frequently in adulthood among the five known genetic groups. TTVs of group 1were detectable in infants aged> 6 months. Its frequency increased with age and was 10-15% in adulthood. TTVs of group 3 comprised the greatest number of genotypes (15 genotypes including SENV of genotypes A to H), were coinfected with TTVs of group 1 and/or TTVs of group 4 in adulthood. Presently, we have established a real-time detection method for quantitating TTVs of group 1,2,3,4 or 5 with an attempt to apply it to clinical surveys.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Okamoto H.: "Genomic and evolutionary characterization of TT virus (TTV) in tupaias and comparison with species-specific TTVs in humans and non-human primates"Journal of General Virology. 82. 2041-2050 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okamoto H.: "Heterogeneous distribution of TT virus of distinct genotypes in multiple tissues from infected humans"Virology. 288. 358-368 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Peng YH.: "Analysis of the entire genomes of thirteen TT virus variants classifiable into the fourth and fifth genetic groups, isolated from viremic infants"Archives of Virology. 147. 21-41 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okamoto H.: "Genomic characterization of TT viruses (TTVs) in pigs, cats and dogs and their relatedness with species-specific TTVs in primates and tupaias"Journal of General Virology. 83. 1291-1297 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kato S.: "Helicobacter pylori and TT virus prevalence in Japanese children"Journal of Gastroenterology. 38. 1126-1130 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okamoto H.: "TTV (TT virus or its official name, Torque Teno virus) : molecular virology and clinical implications"Perspectives in Medical Virology. 10. 241-254 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okamoto H, et al.: "Genomic and evolutionary characterization of TT virus (TTV) in tupaias and comparison with species-specific TTVs in humans and non-human primates."J Gen Virol. 82. 2041-2050 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okamoto H, et al.: "Heterogeneous distribution of TT virus of distinct genotypes in multiple tissues from infected humans."Virology. 288. 358-368 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Peng YH, et al.: "Analysis of the entire genomes of thirteen TT virus variants classifiable into the fourth and fifth genetic groups, isolated from viremic infants."Arch Virol. 147. 21-41 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okamoto H, et al.: "Genomic characterization of TT viruses (TTVs) in pigs, cats and dogs and their relatedness with species-specific TTVs in primates and tupaias."J Gen Virol. 83. 1291-1297 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kato S, et al.: "Helicobacter pylon and TT virus prevalence in Japanese children."J Gastroenterol. 38. 1126 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okamoto H, et al.: "TTV (TT virus or its official name, Torque Teno virus) : molecular virology and clinical implications."Perspectives in Medical Virology. 10. 241-254 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kato S.: "Helicobacter pylori and TT virus prevalence in Japanese children"Journal of Gastroenterology. 38. 1126-1130 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Okamoto H.: "TTV (TT virus or its official name, Torque Teno virus) : molecular virology and clinical implications"Perspectives in Medical Virology. 10. 241-254 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Peng Y. H.: "analysis of the entire genomes of thirteen TT virus variants classifiable into the fourth and fifth genetic groups, isolated from viremic infants"Archives of Virology. 147. 21-41 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Okamoto H.: "Genomic characterization of TT viruses (TTVs) in pigs, cats and dogs and their relatedness with species-specific TTVs in primates and tupaias"Journal of General Virology. 83. 1291-1297 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yokoyama, H.: "Pathological changes of renal epithelial cells in mice transgenic for the TT virus ORF1 gene"Journal of General Virology. 83. 141-150 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takahashi, M.: "TT virus distributed in various leukocyte subpopulations at distinct levels, with the highest viral load in granulocytes"biochemical and Biophysical Research Communications. 290. 242-248 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tokita, H.: "High TT virus load as an independent factor associated with the occurrence of heaptocellular carcinoma among patients with hepatitis C-related chronic liver disease"Journal of Medical Virology. 67. 501-509 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ohto, H.: "TT virus infection during childhood"Transfusion. 42. 892-898 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Okamoto H.: "Genomic and evolutionary characterization of TT virus (TTV) in tupaias and comparison with species-specific TTVs in humans and non-human primates"Journal of General Virology. 82. 2041-2050 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Okamoto H.: "Heterogeneous distribution of TT virus of distinct genotypes in multiple tissues from infected humans"Virology. 288. 358-368 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Muljono D.H.: "Molecular epidemiology of TT virus (TTV) and characterization of two novel TTV genotypes in Indonesia"Archives of Virology. 146. 1249-1266 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shibayama T.: "Inverse relationship between the titre of TT virus DNA and the CD4 cell count in patients infected with HIV"AIDS. 15. 563-570 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Asabe S.: "Phosphorylation of serine-rich protein encoded by open reading frame 3 of the TT virus genome"Biochemical and Biophysical Research Communications. 286. 298-304 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tokita H.: "Influence of TT virus on the clinical course of alcoholic liver disease"Hepatology Research. 19. 180-193 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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