自己幹細胞を用いた自己組織再生誘導型バイオ人工臓器

• Project/Area Number: 13358016
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 展開研究
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Kyoto University
• Principal Investigator: 清水 慶彦 京都大学, 再生医科学研究所, 教授 (00027111)
• Co-Investigator(Kenkyū-buntansha): 岩田 博夫 京都大学, 再生医科学研究所, 教授 (30160120) ; 中村 達雄 京都大学, 再生医科学研究所, 助教授 (70227908)
• Project Period (FY): 2001 – 2003
• Project Status: Completed (Fiscal Year 2003)
• Budget Amount: ¥46,800,000 (Direct Cost: ¥36,000,000、Indirect Cost: ¥10,800,000); Fiscal Year 2003: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000); Fiscal Year 2002: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000); Fiscal Year 2001: ¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
• Keywords: 気管 / 組織工学 / 神経 / 再生 / コラーゲン / 足場 / 細胞外マトリックス / マトリックス / 再生医学 / 自己幹細胞 / 胆管 / 神経再生 / 大腸 / 場の理論 / 再生医療 / 食道

Research Abstract

1.骨髄由来の自己間葉系幹細胞の臨床応用に用いて、その分化増殖のメカニズムと病変部位における効果が大型動物で判明した。
2.この自己組織再生誘導型バイオ人工臓器は
人工血管
人工神経
人工腸管
人工気管
人工内耳
人工喉頭
として欠損部位を形態的にも機能的に再生することが大型動物で確かめられた。
3.自己幹細胞による組織再構築のメカニズムとしては、組織再生の足場となる細胞外マトリックスの役割が重要であり、細胞の分化誘導をつかさどるsignal moleculeは局所における自己の分泌機能(この多くは誘走してくる白血球系の細胞による)が大きく関与している可能性が強く示唆された。
4.人体の全ての臓器が再生医学の手法で再構築できるわけではないが、末梢神経や結合織は、このin situ Tissue Engineeringの手法で再生させうることが確かめられた。
5.これらのうち人工気管、人工神経は大学の倫理委員会の審査を経て、2002年より既に患者に使用されて、良好な結果を得ている。

Research Products

• [Publications] Nakamura, T: "Long-term implantation test and tumorigenicity of polyvinyl alcohol hydrogel plates."J Biomed Mater Res. 56(2). 289-296 (2001)
• [Publications] Nakamura, T.: "Mechanism of burn injury during magnetic resonance imaging (MRI)-simple loops can induce heat injury."Front Med Biol Eng. 11(2). 117-129 (2001)
• [Publications] Iwakura, A.: "Gelatin sheet incorporating basic fibroblast growth factor enhances healing of devascularized sternum in diabetic rats."Circulation. 104(12suppl 1). I-325-I-329 (2001)
• [Publications] Iwakura, A.: "Pericardial fluid from patients with ischemic heart disease induces myocardial cell apoptosis via an oxidant stress-sensitive p38 mitogen-activated protein kinase pathway."J Mol Cell Cardiol. 33(3). 419-430 (2001)
• [Publications] Kawaguchi, S.: "Mechanical properties of artificial tracheas composed of a mesh cylinder and a spiral stent."Biomaterials. 22(23). 3085-3090 (2001)
• [Publications] Kobayashi, H.: "In vivo evaluation of a new sealant material on a rat lung air leak model."J Biomed Mater Res. 58(6). 658-665 (2001)
• [Publications] Shimizu, M.: "Chromosome 13 locus, Pbd2, regulates bone density in mice."J. Bone Miner Res.. 16. 1972-1982 (2001)
• [Publications] Toba, T.: "Regeneration of canine peroneal nerve with the use of a polyglycolic acid-collagen tube filled with laminin-soaked collagen sponge : A comparative study of collagen sponge and collagen fibers as filling materials for nerve conduits."J Biomed Mater Res. 58(6). 622-630 (2001)
• [Publications] 藤川孝満: "三次元位置システムによる人工材料を用いた再生過程の評価"J Clin Physic Ther. 3. 17-22 (2001)
• [Publications] 藤川孝満: "PGA-コラーゲンチューブによる末梢神経再生の評価"J Clin Physic Ther. 3. 27-29 (2001)
• [Publications] 藤川孝満: "ポリグリコール酸(PGA)-コラーゲン複合チューブによる末梢神経再生の評価とリハビリテーションの検討"滋賀県理学療法士会会誌. 21. 50-55 (2001)
• [Publications] Hori, Y.: "Experimental study on in situ tissue engineering of the stomach by acellular collagen sponge scaffol dgrafting"ASAIO J. 47(3). 206-210 (2001)
• [Publications] Hori, Y.: "Experimental study on tissue engineering of the small intestine by acellular collagen sponge scaffold grafting."Int J Artif Organs. 24(1). 50-54 (2001)
• [Publications] Matsumoto, K.: "A gelatin coated collagen-polyglycolic acid composite membrane as a dural substitute."ASAIO J. 47(6). 641-645 (2001)
• [Publications] Liu, Y.: "Tracheal allotransplantation in beagle dogs without immunosuppressants."Ann Thorac Surg. 72(4). 1190-1194 (2001)
• [Publications] 清水慶彦: "再生医学"からだの科学. 217. 2-8 (2001)
• [Publications] 清水慶彦: "神経再生の最先端"臨床外科. 56(1). 45-52 (2001)
• [Publications] 清水慶彦: "再生医学の新しい展開"月刊新医療. 28(1). 124-127 (2001)
• [Publications] 清水慶彦: "再生医学の展望"最新医学. 56(1). 92-97 (2001)
• [Publications] 清水慶彦: "再生組織工学の現状"炎症・再生. 21(1). 29-37 (2001)
• [Publications] 清水慶彦: "末梢神経の組織工学"医学のあゆみ. 196(5). 362-366 (2001)
• [Publications] 清水慶彦: "再生医療の現状と将来の展望"実験医学. 19(15). 204-208 (2001)
• [Publications] 清水慶彦: "新再生を目指す再生医工学"Biotherapy. 15(2). 127-132 (2001)
• [Publications] 清水慶彦: "再生医学"蛍雪時代・臨時増刊. 4. 41-43 (2001)
• [Publications] 清水慶彦: "末梢神経の再生"医学のあゆみ. 199(13). 1101-1105 (2001)
• [Publications] 中村達雄: "軟組織のTissue Engineering"遺伝子医学. 18(4). 123-130 (2001)
• [Publications] 赤川安正: "再生医療の最先端が補綴治療の未来を示す"補綴臨床. 34(1). 8-28 (2001)
• [Publications] 清水慶彦: "再生医学の最先端の課題"月刊新医療. 29. 114-116 (2002)
• [Publications] 清水慶彦: "神経再生-現状と展望"外科治療. 86. 38-44 (2002)
• [Publications] 清水慶彦: "神経再生"小児内科. 34. 90-93 (2002)
• [Publications] 清水慶彦: "再生医療の現状と今後の課題"バイオマテリアル-生体材料-. 20. 175-181 (2002)
• [Publications] Yamamoto, T.: "Transcatheter arterial embolization using poly-L-lactic acid microspheres"Radiation Medicine. 21. 150-154 (2003)
• [Publications] 清水慶彦: "再生医学・医療のフロンティア"週刊医学界新聞. 第2468号. (2002)
• [Publications] 清水慶彦: "気道系の再生"気管支学. 24. 589-594 (2002)
• [Publications] 中村達雄: "消化器・呼吸器の再生"週刊医学界新聞. 第2505号.
• [Publications] 中村達雄: "ここまで進んだ再生医学・医療は補綴治療を根底から変える"補綴臨床. 35. 600-617 (2002)
• [Publications] Hagiwara, A.: "Clinical application of PGA-tube for regeneration of intrapelvic nerves during extended surgery for intrapelvic recurrent rectal cancer."Gan To Kagaku Ryoho. 29. 2202-2204 (2002)
• [Publications] Ueda, H.: "Use of collagen sponge incorporating transforming growth factor-β1 to promote bone repair in skull defects in rabbits."Biomaterials. 23. 1003-1010 (2002)
• [Publications] Komuro, H.: "Application of collagen sponge scaffold to muscular defects of the esophagus : An experimental study in piglets."J Pediatr Surg. 37. 1409-1413 (2002)
• [Publications] 茂野啓示: "歯科(永久・歯根膜、顎骨)の再生"週刊医学界新聞. 第2513号. (2002)
• [Publications] Shigeno, K.: "Regenerative repair of the mandible using a collagen sponge containing TGF-β1."The International Journal of Artificial Organs. 25. 1095-1102 (2002)
• [Publications] Toba, T.: "Influence of dehydrothermal crosslinking on the growth of PC-12 cells cultured on laminin-coated collagen."ASAIO J. 48. 17-20 (2002)
• [Publications] Toba, T.: "Evaluation of peripheral nerve regeneration across an 88-mm gap using a polyglycolic acid (PGA) collagen nerve conduit filled with laminin-soaked collagen sponge in dogs."J Artif Organs. 25. 230-237 (2002)
• [Publications] Hayakawa, K.: "Iodinated contrast medium-induced potassium release ; The effect of mixing ratios."Radiation Medicine. 20. 195-199 (2002)
• [Publications] Hori, Y.: "Experimental study on tissue engineering of the small intestine by mesenchymal stem cell seeding."J Surg Res. 102. 156-160 (2002)
• [Publications] Hori, Y.: "Functional Analysis of the tissue-engineered stomach wall."Artif Organs. 26. 868-872 (2002)
• [Publications] Liu, Y.: "New type of tracheal bioartificial organ treated with detergent : Maintaining cartilage viability is necessary for successful immunosuppressant-free allotransplantation."ASAIO J. 48. 21-25 (2002)
• [Publications] 鳥羽紀成: "再生組織工学の現状と呼吸器疾患への展望"Annual Review 呼吸器2002. 1-6 (2002)
• [Publications] 萩原明於: "再発直腸癌手術の神経合併切除における新しい神経機能再建"治療(J.Therap.). 84. 158-159 (2002)
• [Publications] 清水慶彦: "組織工学による再生医学"The Lung perspectives. 11. 51-54 (2003)
• [Publications] 清水慶彦: "再生医療の成り立ちとそのさまざまな方法"日本医師会雑誌. 129. 299-302 (2003)
• [Publications] 清水慶彦: "再生医学の現状と展望-再生医学の歯科への取り組み-"日本歯科医学会誌. 22. 82-85 (2003)
• [Publications] 清水慶彦: "再生医学のトピックスとその気管食道領域への応用"日本気管食道科学会会報. 54. 59-63 (2003)
• [Publications] 中村達雄: "どうなる?2013年の再生医療と歯科補綴り?"歯科技工. 31. 59-65 (2003)
• [Publications] 中村達雄: "呼吸器疾患の再生医療"日本医師会雑誌. 129. 369-372 (2003)
• [Publications] 中村達雄: "呼吸器疾患の再生医療"日本医師会雑誌. 129. 369-372 (2003)
• [Publications] Nakamura, T.: "Clinical application of nerve conduits consisting of a polyglycolic acid (PGA)-collagen composite tube filled with collagen sponge."Connective Tissue.. 35. 53-57 (2003)
• [Publications] 中村達雄: "再生医療の技術的側面・Tissue Engineering."CLINICAL NEUROSCIENCE. 21. 1141-1145 (2003)
• [Publications] Ito, T.: "Biodegradation of Polyglycolic Acid-Collagen Composite Tubes for nerve Guide in the Peritoneal Cavity."ASAIO Journal. 49. 417-421 (2003)
• [Publications] Ito, T.: "Regeneration of hypogastric nerve using a polyglycolic acid (PGA)-collagen nerve conduit filled with collagen sponge proved electrophysiologically in a canine model."Int J Artif Organs.. 26. 245-251 (2003)
• [Publications] 伊藤忠雄: "人工神経ガイドチューブの腹腔内における分解・吸収性に関する検討"日本炎症・再生医学会雑誌. 23. 275-278 (2003)
• [Publications] 稲田有史: "Causalgia (CRPS Type II)の外科的治療-Polyglycolic-acid Collagen(PGA-C) tubeによる生体内再生治療-"PAIN RESEARCH(日本疼痛学会誌). 18. 25 (2003)
• [Publications] Ueda, H.: "Repairing of rabbit skull defect by dehydrothermally crosslinked collagen sponges incorporating transforming growth factor β1"Journal of Controlled Release. 88. 55-64 (2003)
• [Publications] Ueda, H.: "Repairing of rabbit skull defect by TGF-β1-incorporated collagen sponges of different thickness."Journal of Biomedical Engineering. 15. 1-7 (2003)
• [Publications] Ueda, H.: "Polyhydroxyalkanonate derivatives in current clinical applications and trials."Advanced Drug Delivery Review.. 55. 501-518 (2003)
• [Publications] Okamoto, T.: "Cartilage regeneration using slow release of bone morphogenetic protein-2 from a gelatin sponge to treat experimental canine tracheomalacia : Apreliminary report."ASAIO Journal.. 49. 63-69 (2003)
• [Publications] Liu, Y.: "Experimental study of Blood Typing in Immunosuppressant-free Tracheal Transplantation in Dogs."Thorac Cardiov Surg. 51. 216-220 (2003)
• [Publications] Kanemaru, S.: "Regeneration of the vocal cord using autologous mesenchymal stem cells."Ann Otol Rhinol Laryngol. 112. 915-920 (2003)
• [Publications] Kanemaru, S.: "Recurrent laryngeal nerve regeneration by tissue engineering."Ann Otol Rhinol Laryngol. 112. 492-498 (2003)
• [Publications] Kanemaru, S.: "Regeneration of the mastoid air cells in clinical applications."Acta Otolayngol. (in press). (2003)
• [Publications] Kuremoto, K.: "Experimental study of regeneration of tooth pulp using in situ Tissue Engineering."Tissue Enginering. 9. 845-846 (2003)
• [Publications] 呉本晃一: "in situ Tissue Engineeringによる歯髄再生のための基礎的研究"炎症・再生. 23. 488 (2003)
• [Publications] 桜井靖久: "再生医療の現状と将来展望"日本医師会雑誌. 129. 285-298 (2003)
• [Publications] Terada, Y.: "Capillary endothelial thrombomodulin expression and fibrin deposition in rats with continuous and bolus lipopolysaccharide administration."Lab Invest. 83. 1165-1173 (2003)
• [Publications] 鳥羽紀成: "末梢神経の再生医療"日本医師会雑誌. 129. 323-327 (2003)
• [Publications] Toba, T.: "Experimental research on peripheral nerve regeneration using PGA-collagen nerve conduit filled with collagen sponge."Connective Tissue. 35. 45-52 (2003)
• [Publications] 鳥羽紀成: "末梢神経"バイオマテリアル-生体材料-Journal of Japanese Society for Biomaterials. 21. 186-187 (2003)
• [Publications] Lee, Y.: "Regeneration of serous membrane on gelatin-processed polyglicolic acid (PGA)-human collagen membrane and its efficacy on the prevention of adhesion."J.Biomed.Mater.Res. 64. 88-92 (2003)
• [Publications] 中原 貴: "In situ Tissue Engineeringによる歯周組織の再生"Inflammation and Regeneration. 23. 116-121 (2003)
• [Publications] Nakahara, T.: "Novel Approach to regeneration of periodontal tissues based on in situ tissue engineering ; Effects of controlled release of basic fibroblast growth factor from a sandwich membrane."Tissue Engineering. 9. 153-162 (2003)
• [Publications] 野村宗吾: "血管再生のための足場材料としての異種血管の力学的性質(Mechanical properties of xenograft used for a scaffold for regeneration of blood vessels)"材料(J.Soc.Mat.Sci., Japan). 52. 309-313 (2003)
• [Publications] 萩原明於: "神経再生チューブを用いた神経再建-直腸がん手術における神経再生チューブの臨床応用-"最新医学. 58. 108-113 (2003)
• [Publications] Hori, Y.: "Effect of basic fibroblast growth factor on vascularization in esophagus tissue engineering."Int J Artif Organs.. 26. 241-244 (2003)
• [Publications] Matsuno, T.: "In vivo osteogenetic potential bone marrow mesenchymal stem cell on β-tricalcium phosphate (β-TCP)/collagen sponge."ASAIO J.. 49. 209 (2003)
• [Publications] Matsuno, T.: "Fabrication of β-TCP/collagen sponge for bone regeneration."Int J Artif Organs. 26. 638 (2003)
• [Publications] 松野智宣: "自家成長因子を用いたβ-TCP/コラーゲンスポンジによる骨再生"炎症・再生. 23. 502 (2003)
• [Publications] Yamamoto, T.: "Transcatheter arterial embolization using poly-L-lactic acid microspheres"Radiation Medicine. 21. 150-154 (2003)
• [Publications] Naito, Y.: "Transplantation of bone marrow stromal cells into the cochlea of chinchillas."Neuro Report. 15. 1-4 (2004)
• [Publications] Nakamura, T.: "Sterilized, freeze-dried amniotic membrane : A useful substrate for ocular surface reconstruction."Invest Ophthalmol Vis Sci. 45. 93-99 (2004)
• [Publications] Okamoto, T.: "Slow release of bone morphogenetic protein (BMP)-2 from a gelatin sponge promotes regeneration of tracheal cartilage."J Thorac Cardiovasc Surg.. 127(2). 329-334 (2004)
• [Publications] 中村達雄: "食道.再生医工学の最先端"シーエムシー出版. (2002)
• [Publications] 中村達雄: "「ここまで進んだ再生医療の実際」場の理論を用いた食道の再生(田畑泰彦/編)"羊土社. 129-134 (2003)
• [Publications] 清水慶彦: "組織工学による再生医学"The Lung perspectives. 11. 51-54 (2003)
• [Publications] 清水慶彦: "再生医療の成り立ちとそのさまざまな方法"日本医師会雑誌. 129. 299-302 (2003)
• [Publications] 清水慶彦: "再生医学の現状と展望-再生医学の歯科への取り組み-"日本歯科医学会誌. 22. 82-85 (2003)
• [Publications] 清水慶彦: "再生医学のトピックスとその気管食道領域への応用"日本気管食道科学会会報. 54. 59-63 (2003)
• [Publications] 中村達雄: "どうなる?2013年の再生医療と歯科補綴?"歯科技工. 31. 59-65 (2003)
• [Publications] 中村達雄: "呼吸器疾患の再生医療"日本医師会雑誌. 129. 369-372 (2003)
• [Publications] Nakamura, T.: "Clinical application of nerve conduits consisting of a polyglycolic acid (PGA)-collagen composite tube filled with collagen sponge"Connective Tissue. 35. 53-57 (2003)
• [Publications] 中村達雄: "再生医療の技術的側面・Tissue Engineering"CLINICAL NEUROSCIENCE. 21. 1141-1145 (2003)
• [Publications] Yamamoto, T.: "Transcatheter arterial embolization using poly-L-lactic acid microspheres"Radiation Medicine. 21. 150-154 (2003)
• [Publications] Ito, T.: "Biodegradation of Polyglycolic Acid-Collagen Composite Tubes for nerve Guide in the Peritoneal Cavity"ASAIO Journal. 49. 417-421 (2003)
• [Publications] Ito, T.: "Regeneration of hypogastric nerve using a polyglycolic acid (PGA)-collagen nerve conduit filled with collagen sponge proved electrophysiologically in a canine model"Int J Artif Organs. 26. 245-251 (2003)
• [Publications] 伊藤忠雄: "人工神経ガイドチューブの腹腔内における分解・吸収性に関する検討"日本炎症・再生医学会雑誌. 23. 275-278 (2003)
• [Publications] 稲田有史: "Causalgia(CRPS Type II)の外科的治療-Polyglycolic-acid Collagen(PGA-C) tubeによる生体内再生治療-PAIN RESEARCH"日本疼痛学会誌. 18. 25 (2003)
• [Publications] Ueda, H.: "Repairing of rabbit skull defect by dehydrothermally crosslinked collagen sponges incorporating transforming growth factor β1"Journal of Controlled Release. 88. 55-64 (2003)
• [Publications] Ueda, H.: "Repairing of rabbit skull defect by TGF-β1-incorporated collagen sponges of different thickness"Journal of Biomedical Engineering. 15. 1-7 (2003)
• [Publications] Ueda, H.: "Polyhydroxyalkanonate derivatives in current clinical applications and trials"Advanced Drug Delivery Review. 55. 501-518 (2003)
• [Publications] Okamoto, T.: "Cartilage regeneration using slow release of bone morphogenetic protein-2 from a gelatin sponge to treat experimental canine tracheomalacia : A preliminary report"ASAIO Journal. 49. 63-69 (2003)
• [Publications] Lee, Y.: "Regeneration of serous membrane on gelatin-processed polyglicolic acid (PGA)-human collagen membrane and its efficacy on the prevention of adhesion"J.Biomed.Mater.Res.. 64. 88-92 (2003)
• [Publications] Kanemaru, S.: "Regeneration of the vocal cord using autologous mesenchymal stem cells"Ann Otol Rhinol Laryngol. 112. 915-920 (2003)
• [Publications] kanemaru, S.: "Recurrent laryngeal nerve regeneration by tissue engineering"Ann Otol Rhinol Laryngol. 112. 492-498 (2003)
• [Publications] Kanemaru, S.: "Regeneration of the mastoid air cells in clinical applications"Acta Otolayngol. (in press).
• [Publications] Kuremoto, K.: "Experimental study of regeneration of tooth pulp using in situ Tissue Engineering"Tissue Engineering. 9. 845-846 (2003)
• [Publications] 呉本晃一: "in situ Tissue Engineeringによる歯髄再生のための基礎的研究"炎症・再生. 23. 488 (2003)
• [Publications] 桜井靖久: "再生医療の現状と将来展望"日本医師会雑誌. 129. 285-298 (2003)
• [Publications] Terada, Y.: "Capillary endothelial thrombomodulin expression and fibrin deposition in rats with continuous and bolus lipopolysaccharide administration"Lab Invest. 83. 1165-1173 (2003)
• [Publications] 鳥羽紀成: "末梢神経の再生医療"日本医師会雑誌. 129. 323-327 (2003)
• [Publications] Toba, T.: "Experimental research on peripheral nerve regeneration using PGA-collagen nerve conduit filled with collagen sponge"Connective Tissue. 35. 45-52 (2003)
• [Publications] 鳥羽紀成: "末梢神経.バイオマテリアル-生体材料-"Journal of Japanese Society for Biomaterials. 21. 186-187 (2003)
• [Publications] Liu, Y.: "Experimental Study of Blood Typing in Immunosuppressant-free Tracheal Transplantation in Dogs"Thorac Cardiov Surg. 51. 216-220 (2003)
• [Publications] 中原 貴: "In situ Tissue Engineeringによる歯周組織の再生"Inflammation and Regeneration. 23. 116-121 (2003)
• [Publications] Nakahara, T.: "Novel approach to regeneration of periodontal tissues based on in situ tissue engineering : Effects of controlled release of basic fibroblast growth factor from a sandwich membrane"Tissue Engineering. 9. 153-162 (2003)
• [Publications] 野村宗吾: "血管再生のための足場材料としての異種血管の力学的性質(Mechanical properties of xenograft used for a scaffold for regeneration of blood vessels)"材料(J.Soc.Mat.Sci., Japan). 9. 309-313 (2003)
• [Publications] 萩原明於: "神経再生チューブを用いた神経再建-直腸がん手術における神経再生チューブの臨床応用-"最新医学. 58. 108-113 (2003)
• [Publications] Hori, Y.: "Effect of basic fibroblast growth factor on vascularization in esophagus tissue engineering"Int J Artif Organs. 26. 241-244 (2003)
• [Publications] Matsuno, T.: "In vivo osteogenetic potential bone marrow mesenchymal stem cell on β-tricalcium phosphate (β-TCP)/collagen sponge"ASAIO J. 49. 209 (2003)
• [Publications] Matsuno, T.: "Fabrication of β-TCP/collagen sponge for bone regeneration"Int J Artif Organs. 26. 638 (2003)
• [Publications] 松野智宣: "自家成長因子を用いたβ-TCP/コラーゲンスポンジによる骨再生"炎症・再生. 23. 502 (2003)
• [Publications] Naito, Y.: "Transplantation of bone marrow stromal cells into the cochlea of chinchillas"Neuro Report. 15. 1-4 (2004)
• [Publications] Nakamura, T.: "Sterilized, freeze-dried amniotic membrane ; A useful substrate for ocular surface reconstruction"Invest Ophthalmol Vis Sci. 45. 93-99 (2004)
• [Publications] Okamoto, T.: "Slow release of bone morphogenetic protein (BMP)-2 from a gelatin sponge promotes regeneration of tracheal cartilage"J Thorac Cardiovasc Surg.. 127(2). 329-334 (2004)
• [Publications] 中村達雄: "「ここまで進んだ再生医療の実際」場の理論を用いた食道の再生"田畑泰彦/編、羊土社. 129-134 (2003)
• [Publications] 清水 慶彦: "再生医学の最先端の課題"月刊新医療. 29. 114-116 (2002)
• [Publications] 清水 慶彦: "神経再生-現状と展望"外科治療. 86. 38-44 (2002)
• [Publications] 清水 慶彦: "神経再生"小児内科. 34. 90-93 (2002)
• [Publications] 清水 慶彦: "再生医療の現状と今後の課題"バイオマテリアル-生体材料-. 20. 175-181 (2002)
• [Publications] 清水 慶彦: "再生医学とは?"月刊junior. 414. 7-11 (2002)
• [Publications] 清水 慶彦: "再生医学・医療のフロンティア"週刊医学界新聞. 2468. (2002)
• [Publications] 清水 慶彦: "気道系の再生"気管支学. 24. 589-594 (2002)
• [Publications] 中村 達雄: "消化器・呼吸器の再生"週刊医学界新聞. 2505. (2002)
• [Publications] 中村 達雄: "ここまで進んだ再生医学・医療は補綴治療を根底から変える"補綴臨床. 35. 600-616 (2002)
• [Publications] 中村 達雄: "どうなる?2013年の再生医療と歯科補綴?"歯科技工. 31. 59-65 (2003)
• [Publications] Hagiwara, A.: "Clinical application of PGA-tube for regeneration of intrapelvic nerves during extended surgery for intrapelvic recurrent rectal cancer"Gan To Kagaku Ryoho. 29. 2202-2204 (2002)
• [Publications] Ueda, H.: "Use of collagen sponge incorporating transforming growth factor-β1 to promote bone repair in skull defects in rabbits"Biomaterials. 23. 1003-1010 (2002)
• [Publications] Ueda, H.: "Repairing of rabbit skull defect by dehydrothermally crosslinked collagen sponges incorporating transforming growth factor β1"J.Control.Release.. (in press).
• [Publications] Ueda, H.: "Pepairing of rabbit skull defect by TGF-β1-incorporated collagen sponges of different thickness"J.Biomed.Eng.. (in press).
• [Publications] Ueda, H.: "Polyhydroxyalkanonate derivatives in current clinical applications and trials"Adv.Drug.Deliver.Rev. (in press).
• [Publications] Komuro, H.: "Application of collagen sponge scaffold to muscular defects of the esophagus : An experimental study in piglets"J.Pediatr.Surg. 37. 1409-1413 (2002)
• [Publications] 茂野 啓示: "歯科(永久・歯根膜、顎骨)の再生"週刊医学界新聞. 2513. (2002)
• [Publications] Shigeno, K.: "Regenerative repair of the mandible using a collagen sponge containing TGF-β1"Int.J.Artif.Organs.. 25. 1095-1102 (2002)
• [Publications] Toba, T.: "Influence of dehydrothermal crosslinking on the growth of PC-12 cells cultured on laminin-coated collagen"ASAIO J.. 48. 17-20 (2002)
• [Publications] Toba, T.: "Evaluation of peripheral nerve regeneration across an 80-mm gap using a polyglycolic acid (PGA) collagen nerve conduit filled with laminin-soaked collagen sponge in dogs"J.Artif.Organs.. 25. 230-237 (2002)
• [Publications] Nakahara, T.: "A novel approach to regeneration of periodontal tissues based on in situ tissue engineering : the effects of bFGF controlled release from a sandwich membrane"Tissue Eng.. (in press).
• [Publications] Hayakawa, K.: "Iodinated contrast medium-induced potassium release : The effect of mixing ratios"Radiat.Med. 20. 195-199 (2002)
• [Publications] Hori, Y.: "Experimental study on tissue engineering of the small intestine by mesenchymal stem cell seeding"J.Surg.Res.. 102. 156-160 (2002)
• [Publications] Hori, Y.: "Functional analysis of the tissue-engineered stomach wall"Artif.Organs.. 26. 868-872 (2002)
• [Publications] Liu, Y: "New Type of tracheal bioartificial organ treated with detergent : Maintaining cartilage viability is necessary for successful immunosuppressant-free allotrausplantation"ASAIO J. 48. 21-25 (2002)
• [Publications] 中村 達雄: "食道"再生医工学の最先端. 170-175 (2002)
• [Publications] 鳥羽 紀成: "再生組織工学の現状と呼吸器疾患への展望"Annual Review 呼吸器2002. 1-6 (2002)
• [Publications] 鳥羽 紀成: "末梢神経の再生医療"日本医師会雑誌. (in press).
• [Publications] Toba, T.: "Peripheral nerve regeneration using a polyglycolic acid (PGA) collagen nerve conduit filled with collagen sponge : experimental research and human application"Connective Tissue. (in press).
• [Publications] 中原 貴: "In situ Tissue Engineering による歯周組織の再生"炎症・再生. (印刷中).
• [Publications] Nakamura, T.: "Long-term implantation test and tumorigenicity of polyvinyl alcohol hydrogel plates"J Biomed Mater Res. 56(2). 289-296 (2001)
• [Publications] Nakamura, T.: "Mechanism of burn injury during magnetic resonance imaging (MRI) -simple loops can induce heat injury"Front Med Biol Eng. 11(2). 117-129 (2001)
• [Publications] Iwakura, A.: "Gelatin sheet incorporating basic fibroblast growth factor enhances healing of devascularized sternum in diabetic rats"Circulation. 104. I-325-I-329 (2001)
• [Publications] Iwakura, A.: "Pericardial fluid from patients with ischemic hear disease induces myocardial cell apoptosis via an oxidant stress-sensitive p38 mitogen-activated protein kinase pathway"J Mol Cell Cardiol. 33(3). 419-430 (2001)
• [Publications] Kawaguchi, S.: "Mechanical properties of artificial tracheas composed of a mesh cylinder and a spiral stent"Biomaterials. 22(23). 3085-3090 (2001)
• [Publications] Kobayashi, H.: "In vive evaluation of a new sealant material on a rat lung air leak model"Biomed Mater Res J. 58(6). 658-665 (2001)
• [Publications] Shimizu, M.: "Chromosome 13 locus, Pbd2, regulates bone density in mice"J. Bone Miner Res.. 16. 1972-1982 (2001)
• [Publications] Toba, T.: "Generation of canine peroneal nerve with the use of a polyglycolic acid-collagen tube filled with laminein-soaked collagen sponge : A comparative study of collagen sponge and collagen fibers as filling materials for nerve conduits"J Biomed Maater Res. 58(6). 622-630 (2001)
• [Publications] 藤川孝満: "三次元位置システムによる人工材料を用いた再生過程の評価"J Clin Physic Ther. 3. 17-22 (2001)
• [Publications] 藤川孝満: "PGA-コラーゲンチューブによる抹消神経再生の評価"J Clin Physic Ther. 3. 27-29 (2001)
• [Publications] 藤川孝満: "ポリグリコール酸(PGA)-コラーゲン複合チューブによる末梢神経再生の評価とリハビリテーションの検討"滋賀県理学療法士会会誌. 21. 50-55 (2001)
• [Publications] Hori, Y.: "Experimental study on tissue engineering of the stomach by acellular collagen sponge scaffold grafting"ASAIO J. 47(3). 206-210 (2001)
• [Publications] Hori, Y.: "Experimental study on tissue engineering of the small intestine by acellular collagen sponge by scaffold grafting"Int J Artif Organs. 24(1). 50-54 (2001)
• [Publications] Matsumoto, K.: "A gelatin coated collagen-polyglycolic acid composite membrane as a dural substitute"ASAIO J. 47(6). 641-645 (2001)
• [Publications] Liu, Y.: "Tracheal allotransplantation in beagle dogs without immunosuppressants"Ann Thorac Surg. 72(4). 1190-1194 (2001)
• [Publications] 清水慶彦: "再生医学"からだの科学. 217. 2-8 (2001)
• [Publications] 清水慶彦: "神経再生の最先端"臨床外科. 56(1). 45-32 (2001)
• [Publications] 清水慶彦: "再生医学の新しい展開"月刊新医療. 28(1). 124-127 (2001)
• [Publications] 清水慶彦: "再生医学の展望"最新医学. 56(1). 92-97 (2001)
• [Publications] 清水慶彦: "再生組織工学の現状"炎症・再生. 21(1). 29-37 (2001)
• [Publications] 清水慶彦: "末梢神経の組織工学"医学のあゆみ. 196(5). 362-366 (2001)
• [Publications] 清水慶彦: "再生医療の現状と将来の展望"実験医学. 19(15). 204-208 (2001)
• [Publications] 清水慶彦: "神経再生をめざす再生医工学"Biotherapy. 15(2). 127-132 (2001)
• [Publications] 清水慶彦: "再生医学"蛍雪時代・臨時増刊. 4. 41-43 (2001)
• [Publications] 清水慶彦: "末梢神経の再生"医学のあゆみ. 199(13). 1101-1105 (2001)
• [Publications] 中村達雄: "軟組織のTissue Engineering"遺伝子医学. 18(4). 123-130 (2001)
• [Publications] 赤川安正: "再生医療の最先端が補綴治療の未来を示す"補綴臨床. 34(1). 8-28 (2001)
• [Publications] Ueda, H.: "Use of collagen sponge incorporating transforming growth factor-β1 to promote bone repair in skull defects in rabbits"Biomaterials. 23(4). 1003-1010 (2002)
• [Publications] 清水慶彦: "再生医学の最先端の課題"月刊新医療. 29(1). 114-116 (2002)
• [Publications] 清水慶彦: "神経再生-現状と展望"外科治療. 86(1). 38-44 (2002)
• [Publications] 清水慶彦: "再生医学・医療のフロンティア"週刊 医学会新聞. 2468. (2002)
• [Publications] 鳥羽紀成: "再生組織工学の現状と呼吸器疾患への展望"Annual Review. 1-6 (2002)
• [Publications] Liu, Y.: "New Type of tracheal bioartificial organ treated with detergent : Maintaining cartilage viability is necessary for successful immunosuppressant-free allotransplantation"ASAIO J. 48(1). 21-25 (2002)
• [Publications] Toba, T.: "Influence of dehydrothermal crosslinking on the growth of PC-12 cells cultured on laminin-coated collagen"ASAIO J. 48(1). 17-20 (2002)
• [Publications] Hori, Y.: "Experimental study on tissue engineering of the small intestine by mesenchymal stem cell seeding"J Surg Res. 102. 156-160 (2002)