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Sustainable Food Production and Environmental Preservation by Nitrogen-Fixing Symbionts in Southeast Asia

Research Project

Project/Area Number 13375009
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section海外学術
Research Field 生物資源科学
Research InstitutionOsaka University

Principal Investigator

HAYASHI Makoto (2004)  Osaka Univ., Eng., Assis.Prof., 大学院・工学研究科, 助手 (30291933)

室岡 義勝 (2001-2003)  大阪大学, 大学院・情報科学研究科, 教授 (60029882)

Co-Investigator(Kenkyū-buntansha) MINAMISAWA Kiwamu  Tohoku Univ., Professor, 大学院・生命科学研究科, 教授 (70167667)
YAMADA Takashi  Hiroshima Univ., Professor, 大学院・先端物質科学研究科, 教授 (40230461)
OZAWA Takashi  Osaka Pref.Univ., Agric., Assoc.Prof., 農学部, 助教授 (20152481)
ABE Mikiko  Kagoshima Univ., Sci., Professor, 理学部, 教授 (00107856)
TAJIMA Shigeyuki  KAGAWA UNIV., Professor, 農学部, 教授 (50116894)
山下 光雄  大阪大学, 大学院・工学研究科, 助教授 (40220347)
Project Period (FY) 2001 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥27,430,000 (Direct Cost: ¥21,100,000、Indirect Cost: ¥6,330,000)
Fiscal Year 2004: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2003: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2002: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2001: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Keywordsnitrogen-fixation / nodule / Southeast Asia / symbiont / leguminous tree / rhizobia / 共生工学 / 遺伝的多様性 / 熱帯マメ科樹木 / 窒素固定共生菌 / 持続的食糧増産 / 環境保全
Research Abstract

For sustainable food production and environmental preservation in Southeast Asia, we surveyed/researched fields there, focusing on symbiotic systems of leguminous plants, their rhizobia, and mycorrhiza. We also conducted yearly project meetings, (Philippines/2001, Malaysia/2002, Vietnam/2003, and Indonesia/2004) with specialists from eight Japanese universities including Osaka U., Tohoku U., U. of Tokyo, Hiroshima U., Kagosima U., Kagawa U., Osaka Prefecture U., and Niigata U., and leading scientists from Thailand, Malaysia, Indonesia, the Philippines, and Vietnam ;. At each meeting, we presented our field and laboratory research data in seminars opened to scientists/students in Southeast Asian countries, with 35 to 70 persons in attendance.
Our major findings for this 4 year project include :
a)Acid-and salt-tolerant rhizobia isolated in Thailand, the Philippines, and Indonesian soils can be utilized for bean production in the acidic soils of Southeast Asia ; soybeans, peanuts, and snap … More beans. Inoculation of nitrogen-fixing rhizobia increased bean yield significantly. Now, inoculated rhizobia are sold at low cost and/or supplied free to farmers in the above countries.
b)Microorganisms stimulating plant growth were isolated in Malaysia, the Philippines, and Vietnam soils. These microorganisms, including phosphate-solubilizing bacteria, nitrogen-fixing microorganisms, cyanobacteria, and Azospririllum, increased crop yields, fruit production, and the protein content in seeds.
c)In Thailand, more than 10% deforestation occurred in the past 10 years ; a serious problem for other Southeast Asian countries too. For reforestation, nitrogen-fixing rhizobia and mycorrhiza were isolated in the rhizosphere. These isolates stimulated growth in leguminous/non-leguminous trees in nutrient poor soil, and are now being used at the nursery stage for forests in Indonesia and Thailand.
d)A Japanese group isolated diazotrophic endophytes from gramineous plants and investigated their colonization and nitrogen fixation. These bacteria made significant contributions to the supply of nitrogen sources, allowing for rice production without chemical fertilizers. Less

Report

(5 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (57 results)

All 2005 2004 2003 2002 2001 Other

All Journal Article (32 results) Book (4 results) Publications (21 results)

  • [Journal Article] Root hair abundant genes LjRH101 and LjRH102 encode peroxidase and xyloglucan endotransglycosylase in Lotus japonicus.2005

    • Author(s)
      T.Maekawa
    • Journal Title

      Journal Bioscience and Bioengineering 99(1)

      Pages: 84-86

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Plastid proteins crucial for symbiotic fungal and bacterial entry into plant roots.2005

    • Author(s)
      H.Imaizumi-Anraku
    • Journal Title

      Nature 433

      Pages: 527-531

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] EspB from enterohaemorrhagic Escherichia coli is a natively partially folded protein.2005

    • Author(s)
      D.Hamada
    • Journal Title

      FEBS Journal 272

      Pages: 756-768

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Expression of a Major House Dust Mite Allergen Gene from Dermatophagoides farinae in Lotus japonicus accession Miyakojima MG-20.2005

    • Author(s)
      Tomoaki Kato
    • Journal Title

      Journal Bioscience and Bioengineering 99(2)

      Pages: 165-168

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Lotus burttii takes a position of the third corner in the Lotus molecular genetics triangle2005

    • Author(s)
      Kawaguchi, M
    • Journal Title

      DNA Reseach (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Plastid proteins crucial for symbiotic fungal and bacterial entry into plant roots.2005

    • Author(s)
      H.Imaizumi-Anraku^*, N.Takeda^*, M.Charpentier, J.Perry, H.Miwa, Y.Umehara, H.Kouchi, Y.Murakami, L.Mulder, K.Vickers, J.Pike, A.Downie, T.Wang, S.Sato, E.Asamizu, S.Tabata, M.Yoshikawa, Y.Murooka, G-J.Wu, M.Kawaguchi, S.Kawasaki, M.Parniske, M.Hayashi(^*These authors contributed equally to this work)
    • Journal Title

      Nature 433

      Pages: 527-531

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Root hair abundant genes LjRH101 and LjRH102 encode peroxidase and xyloglucan endglycosylase in Lotus japonicus.2005

    • Author(s)
      Maekawa T, Hayashi M, Murooka Y.
    • Journal Title

      J.Biosci.Bioeng. 99(1)

      Pages: 84-86

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] EspB from enterohaemorrhagic Escherichia coli is a natively partially folded protein.2005

    • Author(s)
      D.Hamada, T.Kato, T.Ikegami, K.N.Suzuki, M.Hayashi, Y.Murooka, T.Honda, I.Yanagihara.
    • Journal Title

      FEBS Joournal 272

      Pages: 756-768

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Lotus burttii takes a position of the third corner in the Lotus molecular genetics triangle.2005

    • Author(s)
      Kawaguchi, M.^*, Pedrosa-Harand, A.^*, Yano, K.^*, Hayashi, M., Murooka, Y., Saito, K., Namai, K., Nishida, H., Shibata, D., Sato, S., Tabata, S., Sandal, N, Stougaard, J., Bachmair, A., Grant, WF.(^*These authors contributed equally to this work)
    • Journal Title

      DNA Res. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Root hair abundant genes LjRH101 and LjRH102 encode peroxidase and xyloglucan endglycosylase in Lotus japonicus.2005

    • Author(s)
      T.Maekawa
    • Journal Title

      Journal Bioscience and Bioengineering 99(1)

      Pages: 84-86

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Expression of a Major House Dust Mite Allergen Gene from Dermatophagoides farinae in Lotus japonicus accession Miyakojima MG-20.2005

    • Author(s)
      Tomoaki Kato
    • Journal Title

      Journal Bioscience and Bioengineering (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Expression islands clustered on the symbiosis island of the Mesorhizobium loti genome.2004

    • Author(s)
      Uchiumi, T.
    • Journal Title

      Journal of Bacteriology 186(8)

      Pages: 2439-2448

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Transgenic Lotus japonicus with an ethylene receptor gene Cm-ERS1 /H70A enhances formation of infection treads and nodule primordia.2004

    • Author(s)
      N.Nukui
    • Journal Title

      Plant and Cell Physiology 45

      Pages: 427-435

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Improvement of simazine degradation by inoculation of corn and soybean plants with rhizobacteria.2004

    • Author(s)
      Ozawa, T.
    • Journal Title

      Soil Science Plant Nutrition 50

      Pages: 1295-1299

    • NAID

      110001720415

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Proteomic analysis on symbiotic differentiation of mitochondria in soybean nodules.2004

    • Author(s)
      L.T-P Hoa
    • Journal Title

      Plant and Cell Physiology 45(3)

      Pages: 300-308

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Pollen development and tube growth are affected in the symbiotic mutant of Lotus japonicus, crinkle.2004

    • Author(s)
      M.Tansengco
    • Journal Title

      Plant and Cell Physiology 45(5)

      Pages: 511-520

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Novel endophytic nitrogen-fixing clostridia from the grass Miscanthus sinensis as revealed by terminal restriction fragment length polymorphism analysis.2004

    • Author(s)
      Miyamoto, T.
    • Journal Title

      Applied Environmental Microbiology 70

      Pages: 6580-6586

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression islands clustered on the symbiosis island of the Mesorhizobium loti genome.2004

    • Author(s)
      Uchiumi, T., Ohwada, T., Itakura, M., Mitsui, H., Nukui, N., Dawadi, P., Kaneko, T., Tabata, S., Yokoyama, T., Tejima, T., Saeki, K., Omori, H., Hayashi, M., Maekawa, T., Sriprang, R., Murooka, Y., Tajima, S., Simomura, K., Nomura, M., Suzuki, A., Shimoda, Y., Sioya, K., Abe, M., Minamisawa, K.
    • Journal Title

      J.Bacteriol. 186(8)

      Pages: 2439-2448

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Pollen development and tube growth are affected in the symbiotic mutant of Lotus japonicus, crinkle.2004

    • Author(s)
      M.Tansengco, H.Imaizumi-Anraku, M.Yoshikawa, S.Takagi, M.Kawaguchi, M.Hayashi, Y.Murooka.
    • Journal Title

      Plant Cell Physiol. 45(5)

      Pages: 511-520

    • NAID

      10012938459

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Pollen development and tube growth are affected in the symbiotic mutant of Lotus japonicus, crinkle.2004

    • Author(s)
      M.Tansengco
    • Journal Title

      Plant Cell Physiology 45(5)

      Pages: 511-520

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Enhanced accumulation of Cd^<2+> by a Mesorhizobium sp. transformed with a gene from Arabidopsis thaliana coding for phytochelatin synthase.2003

    • Author(s)
      R.Sriprang
    • Journal Title

      Applied Environmental Microbiology 69(3)

      Pages: 1791-1796

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] crinkle, a novel symbiotic mutant that affects the infection thread growth and alters the root hair, trichome, and seed development in Lotus japonicus.2003

    • Author(s)
      M.Tansengco
    • Journal Title

      Plant Physiology 131

      Pages: 1054-1063

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Retrotransposon-mediated restoration of Chlorella telomeres : accumulation of Zepp retroransposons at termini of newly formed minichromosomes2003

    • Author(s)
      Y.Yamamoto
    • Journal Title

      Nucleic Acids Research 31

      Pages: 4646-4653

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Enhanced accumulation of Cd^<2+> by a Mesorhizobium sp. transformed with a gene from Arabidopsis thaliana coding for phytochelatin synthase.2003

    • Author(s)
      R.Sriprang, M.Hayashi, H.Ono, M.Takagi, K.Hirata, Y.Murooka.
    • Journal Title

      Appl.Environ.Microbiol. 69(3)

      Pages: 1791-1796

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] crinkle, a novel symbiotic mutant that affects the infection thread growth and alters the root hair, trichome, and seed development in Lotus japonicus.2003

    • Author(s)
      M.Tansengco, M.Hayashi, M.Kawaguchi, H.Imaizumi, Y.Murooka.
    • Journal Title

      Plant Physiol. 131

      Pages: 1054-1063

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A novel bioremediation system for heavy metals using the symbiosis between leguminous plant and genetically engineered rhizobia.2002

    • Author(s)
      R.Sriprang
    • Journal Title

      Journal of Biotechnology 99

      Pages: 279-293

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Complete genomic sequence of nitrogen-fixing symbiotic Bacterium Bradyrhizobium japonicum USDA110.2002

    • Author(s)
      Kaneko, T.
    • Journal Title

      DNA Research 9

      Pages: 189-197

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A novel bioremediation system for heavy metals using the symbiosis between leguminous plant and genetically engineered rhizobia.2002

    • Author(s)
      R.Sriprang, M.Hayashi, M.Yamashita, H.Ono, K.Saeki, Y.Murooka.
    • Journal Title

      J.Biotechnol. 99

      Pages: 279-293

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] High yield expression and single step purification of human thionein/metallothionein.2001

    • Author(s)
      S.-H.Hong
    • Journal Title

      Protein Expression and Purification 21(1)

      Pages: 243-250

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Construction of a genetic linkage map of the model legume Lotus japonicus using an intraspecific F_2 population.2001

    • Author(s)
      M.Hayashi
    • Journal Title

      DNA Research 8

      Pages: 301-310

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Construction of a genetic linkage map of the model legume Lotus japonicus using an intraspecific F_2 population.2001

    • Author(s)
      M.Hayashi, A.Miyahara, S.Sato, T.Kato, M.Yoshikawa, M.Taketa, M.Hayashi, A.Pedrosa, R.Onda, H.Imaizumi-Anraku, A.Bachmair, N.Sandal, J.Stougaard, Y.Murooka, S.Tabata, S.Kawaguchi, K.Harada.
    • Journal Title

      DNA Research 8

      Pages: 301-310

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] High yield expression and single step purification of human thionein/metallothonein.2001

    • Author(s)
      S.-H.Hong, M.Toyama, W.Maret, Y.Murooka.
    • Journal Title

      Protein Expr.Purif. 21(1)

      Pages: 243-250

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 難培養微生物研究の最新技術(工藤俊章/大熊盛他監修)2004

    • Author(s)
      南澤 究
    • Publisher
      シーエムシー出版
    • Related Report
      2004 Annual Research Report
  • [Book] Biotechnology in Agriculture and Forestry, Vol.52 (eds. Nagata/Tabata)2003

    • Author(s)
      K.Harada(共著)
    • Publisher
      Springer-Verlag, Berlin, Heidelberg
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Biotechnology in Sustainable Biodiversity and Food Security (ed. B.N.Prasad)2002

    • Author(s)
      Y.Murooka(共著)
    • Publisher
      Science Publishers Inc., Plymouth, UK
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 生物工学実験書(改訂版)(日本生物工学会編)2002

    • Author(s)
      室岡義勝(編集委員長)
    • Publisher
      培風館
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] R.Sriprang: "Enhanced accumulation of Cd^<2+> by a Mesorhizobium sp. transformed with a gene from Arabidopsis thaliana coding for phytochelatin synthase"Applied and Environmental Microbiology. 69(3). 1791-1796 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Tansengco: "Crinkle, a novel symbiotic mutant that affects the infection thread growth and alters the root hair, trichome, and seed development in Lotus japonicus"Plant Physiology. 131. 1054-1063 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 室岡義勝: "共生工学による環境修復"日本農芸化学会誌. 77(2). 146-149 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Uchiumi, T: "Expression islands clustered on symbiosis island of Mesorhizobium loti genome"Journal of Bacteriology. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 室岡義勝: "高機能プロバイオティクスとしての乳酸菌L137株"日本乳酸菌学会誌. 14(2)(in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] P.Kiatpapan (共著): "ACS Symposium Series 862 Fermentation Biotechnology"American Chemical Society, Washington, DC (ed.B.C.Saha). 221-232 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 平山令明 (翻訳・共訳): "タンパク質ハンドブック"丸善(株). 540 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Toyama, et al.: "Alteration of substrate specificity of cholesterol oxidase from Streptomyces sp. by site-directed mutagenesis"Protein Engineering. 15(6). 477-483 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Toyama, et al.: "Interactions of arsenic with human metallothionein-2"Journal of Biochemistry. 132. 217-221 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Y.Murooka, et al.: "Variation of the amino acid content of Arabidopsis seeds by expressing soybean aspartate aminotransferase gene"Journal of Bioscience and Bioengneering,. 94(3). 225-230 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] R.Sriprang, et al.: "A novel bioremediation system for heavy metals using the symbiosis between leguminous plant and generically engineered rhizobia"Journal of Biotechnology. 99. 279-293 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] N.Yoshida, et al.: "Bacterium-based heavy metal biosorbents : Enhanced uptake of cadmium by E. coli expressing a metallothionein fused to β-galactosidase"BioTechniques. 32(3). 551-558 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] P.Kiatpapan, et al.: "Genetic manipulation system in propionibacteria"Journal of Bioscience and Bioengineering. 93(1). 1-8 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 室岡義勝(編集委員長): "生物工学実験書(改訂版)"日本生物工学会編、培風館. 480 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.-H.Hong: "High yield expression and single step purification of human thionein/ metallothionein"Protein Expression and Purification. 21(1). 243-250 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] H.Kobayashi: "DNA synthesis and fragmentation in bacteroids during Astragalus sinicus root nodule development"Biosc. Biotech. Biochem. 65(3). 510-515 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.Nanda: "Characterizations of acetic acid bacteria in traditional acetic acid fermentation of rice vinegar (komesu) and unpolished rice vinegar (kurosu) produced in Japan"Applied and Environmental Microbiology. 67(2). 986-990 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] P.Kiatpapan: "Construction of an expression vector for propionibacteria and its use in production of 5-aminolevulinic acid by Propionibacterium freudenreichii"Appl. Microbiol. Biotechnol.. 56. 144-149 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y.Murooka: "Genetic design of stable metal-binding biomolecules, oligomeric metallothioneins"Biocatalysis and Biotransformation. 19. 399-412 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] P.Kiatpapan: "Heterologous expression of a gene encoding cholesterol oxidase in probiotic strains of Lactobacillus plantarum and Propionibacterium freudenreichii under the control of native promoters"J. Biosci. Bioeng.. 92(5). 459-465 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] S.Nishikawa, Y.Murooka: "Biotechnology & Genetic Engineering Reviews. Vol. 18"Intercept, Andover, England (ed. S. E. Harding). 149-170 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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