| Project/Area Number |
13440234
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理
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| Research Institution | Saitama University |
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Principal Investigator |
SATO Naoki Saitama University, Faculty of Science, Professor, 理学部, 教授 (40154075)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2002: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2001: ¥10,900,000 (Direct Cost: ¥10,900,000)
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| Keywords | chloroplast / plastid / endosymbiosis / nucleoid / RNA polymerase / sulfite reductase / cyanobacterium / 葉緑体核様体 / DNA結合タンパク質 / ゲノム進化 |
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| Research Abstract |
The following lines of evidence support our hypothesis on the discontinuous evolution of plastid genomic machinery.
1. T7-type RNA polymerase (RPOT) is localized to only mitochondria. In angiosperms, the RPOT gene was duplicated to produce plastid-localyzed isozyme.
2. PEND protein is a DNA-binding protein principally localized to developing plastids. In wounded tissues, this protein is also localized in the nucleus. This protein is only present in angiosperms, and therefore supposed to be newly added transcription factor in angiosperms.
3. Sulfite reductase (SiR) is a major DNA-binding protein in the nucleoids of pea chloroplasts. The cDNA encoding SiR was isolated from pea. In the moss Physcomitrella patens, SiR was also a major component of plastid nucleoids. In red algal plastids and cyanobacteria, SiR was detected in the nucleoids, but was not the major component. This suggests that the major DNA-binding protein of nucleoid has been replaced during the evolution of plastids.
4. Protein phosphorylation is involved in the regulation of transcription in plastid nucleoids.
5. Computer-assisted analysis revealed a large number of plastid proteins that are supposed to originate from cyanobacterial endosymbionts.
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