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Molecular and cellular analysis of autocrine and paracrine functions of endometrial growth factors

Research Project

Project/Area Number 13440247
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 生物形態・構造
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

TAKAHASHI Sumio  Okayama University, Faculty of Science, Professor, 理学部, 教授 (90144807)

Co-Investigator(Kenkyū-buntansha) OKUDA Kiyoshi  Okayama University, Graduate School of Natural Science and Technology, Professor, 自然科学研究科, 教授 (40177168)
TAKEUCHI Sakae  Okayama University, Faculty of Science, Associate professor, 理学部, 助教授 (20226989)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥14,800,000 (Direct Cost: ¥14,800,000)
Fiscal Year 2003: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 2001: ¥6,900,000 (Direct Cost: ¥6,900,000)
Keywordsendometrium / growth factor / estrogen / progestin / proliferation / differentiation / receptor / 成長因子 / 細胞増殖 / 受容体 / トランスフォーミング成長因子α / 子宮 / 性ステロイドホルモン / 下垂体 / マウス / ウシ
Research Abstract

The growth and functions of uterine endometrial cells are regulated by estrogen and progestins. The actions of these sex steroid hormones are thought to be mediated by growth factors and cytokines produced, within the endometrium. The present study was aimed to clarify molecular and cellular regulatory mechanism of gene expression of growth factors and cytokines in the mouse endometrium. Estradiol stimulated transcription of insulin-like growth factor I (IGF-I), epidermal growth factor (EGF), transforming growth factor a (TGF-α), and TGF-β2 genes, and decreased interleukin-18 (IL-18) mRNA level. Progesterone decreased TGF-β1 and TGF-β3 mRNA levels. Thus, the gene expression of all growth factors studied is regulated by estrogen or progestin. Our in vitro study revealed that IGF-I, EGF and TGF-α stimulated DNA replication of endometrial cells. The IGF-I action in terms of DNA replication was mediated by IGF-I type I receptors through MAPkinase and PI3kinase pathways. Combination of estr … More adiol and progesterone stimulated the DNA replication of endometrial stroma cells, and this stimulatory action of the sex steroids was reduced by an EGF-receptor inhibitor RG13022. This result indicates that the stimulatory action by combination of estradiol and progesterone is mediated through EGF receptor signaling using ligands of EGF family-growth factors. These growth factors regulate proliferation of endometroial cells under stimulatory stimuli of estrogen. Estradiol and TGF-α decreased estrogen receptorα mRNA level, suggesting negative feedback system of estrogen action at the estrogen receptor level. Matrix metaloproteinases (MMPs) are thought to be associated with the growth and remodeling of the endometrium. MMP3, MMP7 and MMP9 mRNA levels were analyzed in mouse endometrium. These MMP mRNA levels increased at estrus, in adult mice. In vitro study estradiol decreased MMP3 mRNA levels at 24 hours. The findings altogether suggest that growth factors, cytokines and MMPs are associated with the growth and functions of uterine endometrium. Less

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Murakami, S.: "Expression and action of hepatocyte growth factor in bovine endometrial stromal and epithelial cells in vitro."Molec.Reprod.Dev.. 60. 472-480 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takeuchi, S.: "Identification of a novel growth hormone (GH) isoform : a possible link between GH and melenocortin systems in the developing chicken eye."Endocrinology. 142. 5158-5166 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujiwara, C.: "Hepatocyte growth factor is a regulator in the proliferation of micro vascular endothelial cells in bovine corpus luteum."J.Reprod.Dev.. 48. 49-55 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sharma, S.: "Gene expression and the physiological role of transforming growth factor-α in the mouse pituitary."Zool.Sci.. 20. 83-89 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Honda, J.: "Insulin-Like Growth Factor-I regulates proopiomelanocortin and growth hormone gene expression in the mouse pituitary gland."J.Endocrinol.. 178. 71-82 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Komatsu, N.: "Epidermal growth factor and transforming growth factor-a stimulate the proliferation of mouse uterine stromal cells."Zool.Sci.. 20. 639-645 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Murakami, S.: "Expression and action of hepatocyte growth factor in bovine endometrial stromal and epithelial cells in vitro"Molecular Reproduction and Development. 60. 472-480 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takeuchi, S.: "Identification of a novel growth hormone (GH) isoform : a possible link between GH and melanocortin systems in the developing chicken eye"Endocrinology. 142. 5158-5166 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujiwara, C.: "Hepatocyte growth factor is a regulator in the proliferation of microvascular endothelial cells in bovine corpus luteum"Journal of Reproduction and Development. 48. 49-55 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sharma, S.: "Gene expression and the physiological role of transforming growth factor-α in the mouse pituitary"Zoological Science. 20. 83-89 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Honda, J.: "Insulin-like growth factor-I regulates Proopiomelanocortin and growth hormone gene expression in the mouse pituitary gland"Journal of Endocrinology. 178. 71-82 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Komatsu, N.: "Epidermal growth factor and transforming growth factor-a stimulate the proliferation of mouse uterine stromal cells"Zoological Science. 20. 639-645 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sharma, S.: "Gene expression and the physiological role of transforming growth factor-α in the mouse pituitary"Zool.Sci.. 20. 83-89 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Honda, J.: "Insulin-Like Growth Factor-I regulates proopiomelanocortin and growth hormone gene expression in the mouse pituitary gland"J.Endocrinol.. 178. 71-82 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Komatsu, N.: "Epidermal growth factor and transforming growth factor-a stimulate the proliferation of mouse uterine stromal cells"Zool.Sci.. 20. 639-645 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Fujiwara, C.: "Hepatocyte growth factor is a regulator in the proliferation of microvascular endothelial cells in bovine corpus luteum"Journal of Reproduction and Development. 48巻. 49-55 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takahashi, S.: "Intrapituitary regulatory system of mammotrophs in the mouse"Archives of Physiology and Biochemistry. 110巻. 34-41 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kakeya, T.: "Induction of mammotroph development by a combination of epidermal growth factor, insulin, and estradiol-17β in rat pituitary tumor GH3 cells"Zoological Science. 20巻. 83-89 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shuko Murakami: "Expression and action of hepatocyte growth factor in bovine endometrial stromal and epithelial cells in vitro"Molecular Reproduction and Development. 60巻. 472-480 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Chikako Fujiwara: "Hepatocyte growth factor is a regulator in the prliferation of microvascular endothelial cells in bovine corpus luetum"Journal of Reproduction and Development. 48巻1号. 49-55 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sumio Takahashi: "Intrapituitary regulatory system of mammotrophs in the mouse"Archives of Physiology and Biochemistry. (印刷中).

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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