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Adhesion and Removal Mechanisms of Composite Soils Formed on the Equipment Surface During Food Processing

Research Project

Project/Area Number 13450319
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 化学工学一般
Research InstitutionOkayama University

Principal Investigator

NAKANISHI Kazuhiro  Okayama Univ., Dept. of Biosci. & Bioeng., Professor, 工学部, 教授 (90026584)

Co-Investigator(Kenkyū-buntansha) IMAMURA Koreyoshi  Okayama Univ., Dept. of Biosci. & Bioeng., Associate Professor, 工学部, 助教授 (70294436)
SAKIYAMA Takaharu  Tokyo Univ. of Marine Sci. & Technol., Dept. of Food Sci. & Technol., Associate Professor, 海洋科学部, 助教授 (70170628)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥15,100,000 (Direct Cost: ¥15,100,000)
Fiscal Year 2003: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2002: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥8,500,000 (Direct Cost: ¥8,500,000)
KeywordsFood manufacturing / Soils / β-Lactoglobulin / Peptides / Adhesion mechanism / Removal mechanism / Hydroxyl radicals / Cleaning rate / 洗浄 / 付着 / タンパク質 / 複合汚れ / フーリエ変換赤外分光分析 / 付着部位
Research Abstract

In food manufacturing processes, proteinaceous soils derived from foods tend to strongly adhere to the サball surface of equipment/pipes and thus a lot of energy and detergents are usually consumed for cleaning of the equipment. Although it is strongly required to save energy and detergents by optimizing the cleaning conditions, few systematic approaches have been made. In this project, the intended to reveal scientifically the mechanism for adsorption of proteinaceous soils onto the metal surfaces and that for removal. As a model proteinaceous soil, we usually adopted β-lactoglobulin (β-Lg) and sometimes used a composite of the protein and tannic acid. Particles and plates of stainless steel were mostly used as a model surface. The findings obtained in this projects are summarized as follows.
1. Interaction peptide regions of β-Lg that directly interact with a stainless steel surface was analyzed by the two methods : 1) peptide fragments of β-Lg digested with protease were first in cont … More act with the surface and peptides strongly interacting were identified and 2) β-Lg that had been adsorbed on the surface was subjected to digestion with protease and then the kinds and composition of the remaining peptides were analyzed. As a result, we found that peptide regions containing plural, acidic amino, acids are responsible for direct interaction with the surface at a pH region lower than neutral pH. With increasing the number of acidic amino acids of the peptide, affinity as well as the amount of adsorptiotn increased. Adsorption isotherms could be explained by taking into consideration the both reversible and irreversible adsorption modes.
2. We developed a novel cleaning method, in which negative electropotential (around -0.2〜-0.8V vs. Ag/AgCl) is added to the metal surface being in contact with dilute H_2O_2 solution to generate hydroxyl radicals and generated radials could quickly decompose proteinaceous soils adsorbed on the surface. The proteinaceous soil strongly adsorbed on a stainless steel surface there found to be removed completely within 2 min at room temperature while the removal rate by caustic cleaning was much slower. The various factors including coexisting salt concentration, potential, and current on the removal kinetics there analyzed in details.
3. Adsorption mechanism of the composite soil composed of β-Lg and tannic acid was analyzed under different conditions ~ using RAS-IR. The H_2O_2-electrolysis cleaning method was quite effective to remove the soil from the surface. Namely, tannic acid was removed effectively even by caustic cleaning while sufficient removal of β-Lg was possible only by the H_2O_2-electrolysis cleaning method at room temperature. Less

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (37 results)

All Other

All Publications (37 results)

  • [Publications] 今村維克: "食品製造プロセスにおける洗浄技術の現状と展望"実用産業情報. 24. 15-21 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Imamura: "Cleaning of a stainless steel surface fouled with protein using a UV-H_2O_2 technique."Journal of Chemical Engineering of Japan. 34. 869-877 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Imamura: "In Situ Analysis of the Removal Behavior of Protein Adsorbed on a Titanium Surface by H_2O_2-Electrolysis Treatment."Langmuir. 18. 8033-8039 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Adachi: "A model for describing the desorption processes of β-lactoglobulin fouled on a stainless steel surface during caustic and enzymatic cleanings."Japan Journal of Food Engineering. 3. 79-81 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Imamura: "Removal of proteinaceous soils using hydroxyl radicals generated by theelectrolysis of hydrogen peroxide."Journal of Colloid and Interface Science. 250. 409-414 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Imamura: "Adsorption Behavior of Methylene Blue and Its Congeners on a Stainless Steel Surface."Journal of Colloid and Interface Science. 245. 50-57 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 中西一弘: "食品製造プロセスで発生する汚れの新規洗浄方法"食品工業. 45(2). 18-24 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 今村維克: "過酸化水素の電気分解を利用した新規洗浄方法"冷凍. 77. 44-49 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 今村維克: "食品製造プロセスにおける新規洗浄方法の原理とその特性"ジャパンフードサイエンス. 42. 27-33 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Imamura: "The kinetics of removal of proteins adsorbed on a stainless steel surface by H_2O_2-electrolysis and factors affecting its performance."Journal of Colloid and Interface Science. 265(9). 49-55 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Imamura: "Contribution of acidic amino acid residues to the adsorption of peptides to a stainless steel surface."Journal of Colloid and Interface Science. 267. 294-301 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Sakiyama: "Adsorption characteristics of bovine serum albumin and its peptide fragments on a stainless steel surface."Colloids and Surfaces B. 33. 77-84 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Nakanishi: "Kinetics of soil removal during cleaning"Encyclopedia of Agricultural, Food, and Biological Engineering. 6 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 中西一弘: "洗浄百科辞典"朝倉書店. 8 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Imamura: "Cleaning of a stainless steel surface fouled with protein using a UV-H2O2 technique."Journal of Chemical Engineering of Japan. 34. 869-877 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Imamura: "In Situ Analysis of the Removal Behavior of Protein Adsorbed on a Titanium Surface by H2O2-Electrolysis Treatment"Langmuir. 18(21). 8033-8039 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Adachi: "A model for describing-the desorption processes of β-lactoglobulin fouled on a stainless steel surface during caustic and enzymatic cleanings."Japan Journal of Food Engineering. 3. 79-81 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Imamura: "Removal of Proteinaceous Soils Using Hydroxyl Radicals Generated by the Electrolysis of Hydrogen Peroxide"Journal of Colloid and Interface Science. 250(2). 409-414 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Imamura: "Adsorption Behavior of Methylene Blue and Its Congeners on a Stainless Steel Surface"Journal of Colloid and Interface Science. 245(1). 50-57 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Imamura: "The kinetics of removal of proteins adsorbed on a stainless steel surface by H2O2-electrolysis and factors affecting its performance."Journal of Colloid and Interface Science. 265(9). 49-55 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Imamura: "Contribution of acidic amino acid residues to the adsorption of peptides to a stainless steel surface."Journal of Colloid and Interface Science. 267. 294-301 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Sakiyama: "Adsorption characteristics of bovine serum albumin and its peptide fragments on a stainless steel surface."Colloids and Surfaces B. 33. 77-84 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Nakanishi: "Kinetics of soil removal during cleaning"Encyclopedia of Agricultural, Food, and Biological Engineering (Marcel Dekker). 552-556 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Imamura: "The kinetics of removal of proteins adsorbed on a stainless steel surface by H_2O_2-electrolysis and factors affecting its performance."Journal of Colloid and Interface Science. 265(9). 49-55 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 中西一弘: "濾過技術の基礎と濾過プロセスの設計"情報機構,東京. 14 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 中西一弘: "洗剤・洗浄百科辞典"朝倉書店. 8 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 中西一弘: "農芸化学の事典"朝倉書店. 8 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Imamura, T.Sakiyama, K.Nakanishi et al.: "Adsorption Behavior of Methylene Blue and Its Congeners on a Stainless Steel Surface"Journal of Colloid and Interface Science. 245(1). 50-57 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Imamura, T.Sakiyama, K.Nakanishi et al.: "Enzymatic Synthesis of N-Acyl-L-amino Acids in a Glycerol-Water System Using Acylase I from Pig Kidney"Journal of American Oil Chemists' Society. 79(1). 41-46 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Imamura, T.Sakiyama, K.Nakanishi et al.: "Influences of the Suspension Method of Thermolysin in Organic Solvents on Enzyme Activity"Japan Journal of Food Engineering. 3(1). 15-22 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Imamura, T.Sakiyama, K.Nakanishi et al.: "Removal of Proteinaceous Soils Using Hydroxyl Radicals Generated by the Electrolysis of Hydrogen Peroxide"Journal of Colloid and Interface Science. 250(2). 409-414 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Imamura, T.Sakiyama, K.Nakanishi et al.: "Water Sorption and Glass Transition Behaviors of Freeze-Dried Sucrose-Dextran Mixtures"Journal of Pharmaceutical Science. 91(10). 2175-2181 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Imamura, T.Sakiyama, K.Nakanishi et al.: "In Situ Analysis of the Removal Behavior of Protein Adsorbed on a Titanium Surface by H_2O_2-Electrolysis Treatment"Langmuir. 18(21). 8033-8039 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 中西一弘, 今村維克, 崎山高明(他: "食品設備・機器事典"産業調査会. 429 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Imamura: "Adsorption Behavior of Methylene Blue and Its Congeners on a Stainless Steel Surface"Journal of Colloid and Interface Science. 245. 50-57 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 中西一弘: "食品製造プロセスで発生する汚れの新規洗浄方法"食品工業. 45(2). 18-24 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 今村維克: "食品製造プロセスにおける洗浄技術の現状と展望"実用産業情報. 24. 15-21 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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