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Novel regenerable cell culture system using signal responsive membrane substrate

Research Project

Project/Area Number 13450326
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 反応・分離工学
Research InstitutionThe University of Tokyo

Principal Investigator

YAMAGUCHI Takeo  The University of Tokyo, Graduate School of Engineering, Associate Professor, 大学院・工学系研究科, 助教授 (30272363)

Co-Investigator(Kenkyū-buntansha) TAKABA Hiromitsu  The University of Tokyo, Graduate School of Engineering, Research Associate, 大学院・工学系研究科, 助手 (80302769)
SAKAI Yasuyuki  The University of Tokyo, Institute of Industrial Science, Associate Professor, 生産技術研究所, 助教授 (00235128)
NAKAO Shin-ichi  The University of Tokyo, Graduate School of Engineering, Professor, 大学院・工学系研究科, 教授 (00155665)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥14,800,000 (Direct Cost: ¥14,800,000)
Fiscal Year 2003: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 2001: ¥5,400,000 (Direct Cost: ¥5,400,000)
KeywordsPlasma-graft polymerization / Tissue engineering / Chemical signal / Supennolecules / Stimuli responsive polymer / 生体類似膜 / 細胞培養 / 環境応答ゲル / ホストゲスト / クラウンエーテル / 新陳代謝
Research Abstract

In recent years, bio-reactors and bio-artificial organs utilizing cellular functions have been developed, although there are still unsolved problems such as that they cannot be used for a long time period. One of the substantial differences between synthetic materials and biomaterials is their ability to self-repair. When some cells die on the artificial material, inflammatory substances are released from the dead cells and damage adjacent living cells, leading to the expansion of the inflammation. In this study, we developed a rapid regenerable cell culture system. Namely, the material can selectively recognize local cell death and subsequently remove the dead cells actively from the material surface, so that the resulting vacant spaces can be regenerated voluntarily and quickly by the growth of surrounding cells. Cells are detached from N-isopropylacrylamide (NIPAM) grafted surface by temperature decreasing below 32℃, because NIPAM changes its properties. In this study, a crown is used as a sensor that recognizes signals from dead cells, and NIPAM is used as an actuator. A designed polymer is a copolymer of NIPAM and Benzo-18-Crown-6-acrylamide (BCAm) with a pendant crown ether receptor. When the crown ether captures specific ion, the LCST shifts to higher temperature, because guest-host complex makes the polymer chain more hydrophilic. Therefore, when the cells die, the polymer surface recognizes potassium ion released from the dead cells, NIPAM hydrates, and selectively detach the dead cells. This study is a pioneering approach that recognizes cell signals and which works in living systems in the novel field of tissue engineering.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] T.Yamaguchi et al.: "Response mechanism of a molecular recognition ion gating membrane"Macromolecules. (In press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yamaguchi, S.Nakao et al.: "Study of SPG membrane emulsification processes for the preparation of monodisperse core-shell microcapsules"J.Colloid Interf.Sci.. 265(1). 187-196 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yamaguchi, S.Nakao et al.: "Prediction and estimation of solvent diffusivity in poly(acrylate) or poly(methacrylate)s"J.Polym.Sci., Polym.Phys.Ed.. 41(12). 1393-1400 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yamaguchi, S.Nakao et al.: "New morphological control for thick, porous membranes with a plasma graft-filling polymerization"J.Polym.Sci., Polym.Chem.Ed.. 41(9). 1216-1224 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yamaguchi, S.Nakao et al.: "Development of a molecular recognition polymer system controlled by thermosensitive polymer chains"Ind.Eng.Chem.Res.. 42(2). 380-385 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yamaguchi, S.Nakao et al.: "Thermo-responsive transport behavior through porous membranes with linear-grafted poly(N-isopropylacrylamide) gates : From flat membranes to core-shell microcapsule membranes"AIChE J.. 49(4). 896-909 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yamaguchi, et al.: "Response mechanism of a molecular recognition ion gating membrane"Macromolecules. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yamaguchi, S.Nakao et al.: "Study of SPG membrane emulsification processes for the preparation of monodisperse core-shell microcapsules"J.Colloid Interf.Sci.. 265(1). 187-196 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yamaguchi, S.Nakao et al.: "Prediction and estimation of solvent diffusivity in poly(acrylate) or poly(methacrylate)s"J.Polym.Sci., Polym.Phys.Ed.. 41(12). 1393-1400 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yamaguchi, S.Nakao et al.: "New morphological control for thick, porous membranes with a plasma graft-filling polymerization"J.Polym.Sci., Polym.Chem.Ed.. 41(9). 1216-1224 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yamaguchi, S.Nakao et al.: "Thermo-responsive transport behavior through porous membranes with linear-grafted poly(N-isopropylacrylamide) gates : From flat membranes to core-shell microcapsule membranes"AIChE J.. 49(4). 896-909 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yamaguchi, S.Nakao et al.: "Development of a molecular recognition polymer system controlled by thermosensitive polymer chains"Ind.Eng.Chem.Res.. 42(4). 380-385 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yamaguchi, S.Nakao, et al.: "Thermo-responsive transport behavior through porous membranes with linear-grafted poly(N-isopropylacrylamide) gates : From flat membranes to core-shell microcapsule membranes"AIChE J. 49. 896-909 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Yamaguchi, S.Nakao, et al.: "Development of a molecular recognition polymer system controlled by thermosensitive polymer chains"Ind.Eng.Chem.Res. 42. 380-385 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Yamaguchi, S.Nakao, et al.: "New morphological control for thick, porous membranes with a plasma graft-filling polymerization"J.Polym.Sci., Polym.Chem.Ed.. 41. 1216-1224 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Yamaguchi, S.Nakao, et al.: "Study of SPG membrane emulsification processes for the preparation of monodisperse core-shell microcapsules"J.Colloid Interf.Sci.. 265. 187-196 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Yamaguchi, S.Nakao, et al.: "Polymer electrolyte membranes with pore-filling structure for a direct methanol fuel cell"Advanced Materials. 15. 1198-1201 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Yamaguchi, S.Nakao, et al.: "Prediction and estimation of solvent diffusivity in poly(acrylate) or poly(methacrylate)s"J.Polym.Sci., Polym.Phys.Ed. 41. 1393-1400 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 山口猛央(編集/植田充美): "ナノバイオテクノロジーの最前線(Frontier of Nano-Biotechnology)、編集/植田充美、「分子認識イオンゲート膜」"CMC出版. 52-61 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Yamaguchi, S.Nakao et al.: "A molecular recognition microcapsule for environmental stimuli-responsive controlled-release"Advanced Materials. 14. 386-389 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Yamaguchi, S.Nakao et al.: "Development of a molecular recognition ion gating membranes and estimation of its pore size control"J. Am. Chem. Soc.. 124. 7840-7846 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Yamaguchi, S.Nakao et al.: "Development of a Molecular Recognition Polymer System Controlled by Thermosensitive Polymer Chains"Ind. Eng. Chem. Res.. 42. 380-385 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Yamaguchi, S.Nakao et al.: "Pore-filling electrolyte membrane-electrode integrated system for Direct Methanol Fuel Cell application"J. Electrochem. Soc.. 149. 1448-1453 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Yamaguchi, S.Nakao et al.: "Plasma-graft Pore-filling Electrolyte Membranes Using a Porous Poly(tetrafluoroethylene) Substrate"Electrochemistr. 70. 950-952 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 山口猛央, 中尾真一: "プラズマグラフト重合法によるPTFE多孔基材を用いたフィリング電解質膜の開発"化学工学論文集. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Yamaguchi, S.Nakao et al.: "Prediction of the solubility of chloroform in acrylate polymer mixtures with inclusion of the hydrogen-bonding effect"J. Phys. Chem. B. 105. 3143-3149 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Yamaguchi, S.Nakao et al.: "Transport phenomena through intercrystalline and intracrystalline pathways of silicalite zeolite membranes"J. Membrane Sci.. 187. 203-212 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Yamaguchi, S.Nakao et al.: "Cut-off of dilute O/W emulsions through a microfiltration membrane"J. Membrane Sci.. 190. 167-178 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Yamaguchi, S.Nakao et al.: "Thermo-responsive core-shell microcapsules with porous membrane and poly (N-isopropylacrylamide) gates"J. Membrane Sci.. 192. 27-39 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Yamaguchi, S.Nakao et al.: "Hollow Fiber Type Membranes Made by Plasma-Graft Filling Polymerization for Chlorinated Organics Removal from Water"J. Membrane Sci.. 194. 217-228 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Yamaguchi, S.Nakao et al.: "Preparation of Small-Sized Monodispersed Thermo-Responsive Core-Shell Microcapsules"Langmuir. 18. 1856-186 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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