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GENERATION AND UTILIZATION OF NOVEL HUMAN ANTIBODY MOLECULES BASED ON GENETIC INFORMATION

Research Project

Project/Area Number 13450337
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 生物・生体工学
Research InstitutionTohoku University

Principal Investigator

KUMAGAI Izumi  TOHOKU UNIVERSITY, GRADUATE SCHOOL OF ENGINEERING, PROFESSOR, 大学院・工学研究科, 教授 (10161689)

Co-Investigator(Kenkyū-buntansha) ASANO Ryutaro  TOHOKU UNIVERSITY, GRADUATE SCHOOL OF ENGINEERING, ASSISTANT PROFESSOR, 大学院・工学研究科, 助手 (80323103)
UMETSU Mitsuo  TOHOKU UNIVERSITY, IMIRM, ASSISTANT PROFESSOR, 多元物質科学研究所, 助手 (70333846)
TSUMOTO Kouhei  TOHOKU UNIVERSITY, GRADUATE SCHOOL OF ENGINEERING, ASSOCIATE PROFESSOR, 大学院・工学研究科, 助教授 (90271866)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥14,400,000 (Direct Cost: ¥14,400,000)
Fiscal Year 2003: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2002: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2001: ¥9,300,000 (Direct Cost: ¥9,300,000)
KeywordsANTIBODY / PHAGE-DISPLAY SYSTEM / MUTAGENESIS / AUTOANTIGENS / CRYTAL STRUCTURE / EXPRESSION SYSTEM / MOLECULAR RECOGNITION / REFOLDING SYSTEM / 抗原抗体相互作用 / 蛋白性抗原 / X線結晶構造解析 / 熱力学的解析 / 変異体 / 非共有結合 / 蛋白質間相互作用
Research Abstract

A major goal of this study is construction of novel preparation system of human antibody molecules based on human antibody genome information by diversity creation of genes, in vitro stable selection via recognition mechanism, and development of preparation system of selected molecules. The results are summarized as follows.
1.Diversity of genes:
DNA primers for specific amplification of human and mouse antibody variable domain genes have been designed, and the experimental conditions have been established. In addition to overlap-extension PCR, experimental conditions for error-prone PCR for randomization of antibody variable domain genes, revealing that concentration of metal ions and control of reaction time are critical for efficient amplification and mutation of the genes.
2.Selection:
Novel selection method on the basis of Fv stabilization under existence of antigens has been established. Using this method, human antibody molecules specific for EGF receptor, NK cell inhibitory recepto … More r, bio-degradable plastic surface, and gold could be selected. A humanized antibody could be functionally improved via random mutation under conditions described in 1 and selection of the phage-displayed phages using target-expressed cells. Selection system using a SPR instrument has been constructed.
3.Preparation system:
For refolding of antibodies and cytokines, their refolding procedure has been improved, and spectroscopic evidence for high refolding efficiency of the additive-induced step-wise dialysis method has been elucidated. Preparation of Fv-fusion protein, e.g. alkaline phosphatase and cytokines, has been established and recombinant bispecific antibody, diabody has been also constructed.
4.Molecular recognition:
Human antibody variable regions, obtained from selection in 2, have been prepared using E. coli expression system, and functional characterization using ITC, QCM and/or SPR has been performed. Selected Fv fragments have also been characterized from structural viewpoints, revealing several novel insights into the mechanism of antibodies for antigen recognition. Less

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (173 results)

All Other

All Publications (173 results)

  • [Publications] Takao Yoda, et al.: "Folding-Unfolding of Goat α-Lactalbumin Studied by Stopped-Flow Circular Dichroism and Molecular- Dynamics Simulations"PROTEINS : Structure, Function, and Genetics. 42. 49-65 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ryoichi Arai, et al.: "Demonstration of a Homogeneous Noncompetitive Immunoassay Based on Bioluminescence Resonance Energy Transfer"Analytical Biochemistry. 289. 77-81 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Michihiro Nakamura, et al.: "Panning of a Phage VH Library Using Nitrocellulose Membranes : Application to Selection of a Human VH Library"Journal of Biochemistry. 129. 209-212 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nobuhiro Kasai, et al.: "Panning of a Phage VH Library Using Nitrocellulose Membranes : Application to Selection of a Human VH Library"Biochem.Biophys.Res.Commun.. 281. 416-423 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mitsunori Shiroishi, et al.: "Structural Evidence for Entropic Contribution of Salt Bridge Formation to a Protein Antigen-Antibody Interaction"The Journal of Biological Chemistry. 276. 23042-23050 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Masahiro Kawahara, et al.: "Replacing Factor-Dependency with that for Lysozyme : Affordable Culture of IL-6-Dependent Hybridoma by Transfecting Artificial Cell Surface Receptor."BIOTECHNOL.BIOENG.. 74. 416-423 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Katsunori Horii, et al.: "Contribution of Thr29 to the Thermodynamic Stability of Goat α-Lactalbumin as Determined by Experimental and Theoretical Approaches"PROTEINS : Structure, Function, and Genetics. 45. 16-29 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Michihiro Nakamura, et al.: "A Visualization Method of Filamentous Phage Infection and Phage-Derived Proteins in Escherichia coli Using Biotinylated Phages"Biochem.Biophys.Res.Commun.. 289. 252-256 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hideaki Kodama, et al.: "Mutated SEA-D227A-conjugated antibodies greatly enhance antitumor activity against MUCI-expressing bile duct carcinoma"Cancer Immunology Immunotherapy. 50. 539-548 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Michihiro Nakamura, et al.: "Phage library panning against cytosolic fraction of cells using quantitative dot blotting assay : application of selected VH to histochemistry"Journal of Immunological Methods. 261. 65-72 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shin-ichi Takemura, et al.: "A mutated superantigen SEA D227A fusion diabody specific to MUC1 and CD3 in targeted cancer immunotherapy for bile duct carcinoma"Cancer Immunology Immunotherapy. 51. 33-44 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Michihiro Nakamura, et al.: "Detection of Biotinylated Proteins in Polyacrylamide Gels Using an Avidin-Fluorescein Conjugate"Analytical Biochemistry. 304. 231-235 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] ARUTO UENO, et al.: "T cell Immunotherapy for Human MK-1-Expressing Tumors Using a Fusion Protein of the Superantigen SEA and Anti-MK-1 scFv Antibody"ANTICANCER RESEARCH. 22. 769-776 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] MASAHIRO KAWAHARA, et al.: "Selection of Highly Productive Mammalian Cells Based on an Inducible Growth Advantage Using an Antibody/Receptor Chimera"JOURNAL OF BIOSCIENCE AND BIOENGINEERING. 93. 399-404 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Michihiro Nakarnura, et al.: "The effect of an agglutogen on virus infection : biotinylated filamentous phages and avidin as a model"FEBS Letters. 520. 77-80 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hideki Watanabe, et al.: "Selection of human antibody fragments on the basis of stabilization of the variable domain in the presence of target antigens"Biochemical and Biophysical Research Communications. 295. 31-36 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shinobu Honzawa, et al.: "A mutated superantigen SEA D227A fusion diabody specific to MUC1 and CD3 in targeted cancer Immunotherapy for bile duct carcinoma"Bioorganic & Medicinal Chemistry. 10. 3213-3218 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kouhei Tsumoto, et al.: "Inhibition of hepatitis C virus NS3 protease by peptides derived from complementarity -determining regions (CDRs) of the monoclonal antibody 8D4"FEBS Letters. 525. 77-82 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ryutaro Asano, et al.: "Antitumor activity of Interleukin-21 prepared by novel refolding procedure from inclusion bodies expressed in Escherichia coli"FEBS Letters. 528. 70-76 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ryutaro Asano, et al.: "Efficient construction of a diabody using a refolding system : anti-carcinoembryonic antigen recombinant antibody fragment"Journal of Biochemistry. 13. 903-909 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 津本浩平, 熊谷 泉: "ファージディスプレイを用いた新規バイオ分子の選択と応用"BIO INDUSTRY. 18. 26-33 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 津本浩平ら: "凝集蛋白質を再生する"蛋白質 核酸 酵素. 46. 1238-1246 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Toshio Kudo ら: "Review - Second generation bispecific antibodies : Applications in cancer immunotherapy"Recent Research Developments in Cancer. 3. 235-255 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 津本浩平ら: "アルギニンを用いた変性タンパク質のリフォールディング"実験医学. 21. 1669-1673 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kouhei Tsumoto, et al.: "Mini Review : Practical considerations in refolding proteins from inclusion bodies"Protein Expression and Purification. 28. 1-8 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Akiko Yokota, et al.: "The role of hydrogen bonding via interfacial water molecules in antigen-antibody complexation : The HyHEL-10-HEL interaction"The Journal of Biological Chemistry. 278. 5410-5418 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Michihiro Nakamura, et al.: "A morphological study of filamentous phage infection of Escherichia coli using biotinylated phages"FEBS Letters. 536. 167-172 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mitsuo Umetsu et al.: "How additives influence the refolding of immunoglobulin-folded proteins in a stepwise dialysis system"The Journal of Biological Chemistry. 278. 8979-8987 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Masahiro Kawahara, et al.: "Bypassing antibiotic selection : positive screening of genetically modified cells with an antigen-dependent proliferation switch"Nucleic Acids Research. 31. e32 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Izumi Kumagai, et al.: "Structural consequences of target site-directed functional alteration of an antibody : The case of anti-lysozyme antibody, HyHEL-10"The Journal of Biological Chemistry. 278. 24929-24936 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mitsunori Shiroishi, et al.: "Human inhibitory receptors ILT2 and ILT4 compete with CD8 for MHC class I binding and bind preferentially to HLA-G"Proc.Natl.Acad.Sci.USA. 100. 8856-8861 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kouhei Tsumoto, et al.: "Immobilized Protein Disulfide Isomerase As an Additive for Refolding of Inclusion Bodies : Application to Antibody Fragments."Protein Engineering. 16. 535-541 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hiroshi Ueda, et al.: "An optimized homogeneous noncompetitive immunoassay based on the antigen-driven enzymatic complementation"Journal of Immunological Methods. 279. 209-218 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mitsuhiro Matsumoto, et al.: "On column refolding and characterization of soluble human interleukin-15 receptor a chain produced in Escherichia coli"Protein Expression and Purification. 31. 64-71 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Koji Iida, et al.: "Stable Expression of the Motor Protein Prestin in Chinese Hamster Ovary Cells"JSME International Journal Series C. 46. 1266-1274 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshikazu Tanaka, et al.: "Structural Implications for heavy metal-induced reversible assembly and aggregation of a protein : Crystal structure and metal binding properties of Pyrococcus horikoshii CutA"FEBS Letters. 556. 167-174 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mitsuo Umetsu, et al.: "Structural characteristics and refolding of in vivo aggregates of archaeon proteins"FEBS Letters. 557. 49-56 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hiroki Hayashi, et al.: "A highly effective and stable bispecific diabody for cancer immunotherapy : cure of xenografted tumors by bispecific diabody and T-LAK cells."Cancer Immunol Immunother. 53. 495-507 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Masahiro Kawahara, et al.: "AMEGA : antigen-mediated genetically modified cell amplification"Journal of Immunological Methods. 284. 187-194 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Akira Okazaki, et al.: "Fucose Depletion from Human IgG1 Oligosaccharide Enhances Binding Enthalpy and Association Rate between IgGI and Fc□RIIIa"Journal of Molecular Biology. (印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoko Nagumo, et al.: "Phase-Display Selection of Antibodies to the Left End of CTX3C Using Synthetic Fragments"Journal of Immunological Methods. (印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshikazu Tanaka, et al.: "Crystal Structure of a Hyperthermophilic CutA from Pyrococcus horikoshii in 3 M Guanidine Hydrochloride : Implication for Guanidine -Side Chain Interactions"Biochem.Biophys.Res.Commun.. (印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 浅野竜太郎ら: "組換え型抗体の現状と将来"Bioindustry. 20. 6-14 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 津本浩平, 熊谷 泉: "凝集タンパク質の再生〜凝集抑制と巻き戻し〜"BIOベンチャー. 32-35 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 荒川 力ら: "タンパク質リフォールディングの救世主:アルギニン"蛋白質核酸酵素. 48. 2310-2317 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 梅津光央ら: "凝集蛋白質の効率的再生と分光学的フォールディング研究の接点"生物物理. (印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 熊谷泉, 津本浩平: "新タンパク質科学実験法 第3巻構造機能解析(上)"東京化学同人. 200 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 津本浩平, 郷田秀一郎, 熊谷泉: "最新酵素利用技術と応用展開"シーエムシー. 500 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 津本浩平, 熊谷 泉: "コンビナトリアルサイエンスの新展開"シーエムシー. 500 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 津本, 熊谷(分担執筆): "化学フロンティア(8)コンビナトリアル・バイオエンジニアリング"化学同人. 180 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 熊谷, 津本(分担執筆): "生物物理学ハンドブッグ"朝倉書店(印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 津本, 熊谷(分担執筆): "ゲノミクス・プロテオミクスの新展開〜生物情報の解析と応用〜"エヌ・ティー・エス(印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 津本浩平, 熊谷 泉: "生体ナノマシンの分子設計"共立出版. 200 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takao Yoda: "Folding-Unfolding of Goat α-Lactalbumin Studied by Stopped-Flow Circular Dichroism and Molecular Dynamics Simulations"PROTEINS : Structure, Function, and Genetics. 42. 49-65 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ryoichi Arai: "Demonstration of a Homogeneous Noncompetitive Immunoassay Based on Bioluminescence Resonance Energy Transfer"Analytical Biochemistry. 289. 77-81 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Michihiro Nakamura: "Panning of a Phage VH Library Using Nitrocellulose Membranes : Application to Selection of a Human VH Library"Journal of Biochemistry. 129. 209-212 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nobuhiro Kasai: "Inhibition of the Hepatitis C Virus NS3 Protease Activity by Fv fragment of Antibody 8D4"BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 281. 416-423 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mitsunori Shiroishi: "Structural Evidence for Entropic Contribution of Salt Bridge Formation to a Protein Antigen-Antibody Interaction THE CASE OF HEN LYSOZYME-HyHEL-l0FvCOMPLEX"The Journal of Biological Chemistry. 276. 23042-23050 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Masahiro Kawahara: "Replacing Factor-Dependency with that for Lysozyme : Affordable Culture of IL-6-Dependent Hybridoma by Transfecting Artificial Cell Surface Receptor."BIOTECHNOL.BIOENG.. 74. 416-423 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Katsunori Hori: "Contribution of Thr29 to the Thermodynamic Stability of Goat α-Lactalbumin as Determined by Experimental and Theoretical Approaches"PROTEINS : Structure, Function, and Genetics. 45. 16-29 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Michihiro Nakamura: "A Visualization Method of Filamentous Phage Infection and Phage-Derived Proteins in Escherichia coli Using Biotinylated Phages"Biochemical and Biophysical Research Communications. 289. 252-256 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hideaki Kodama: "Mutated SEA-D227A-conjugated antibodies greatly enhance antitumor activity against MUCI-expressing bile duct carcinoma"Cancer Immunology Immunotherapy. 50. 539-548 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Michihiro Nakamura: "Phage library panning against cytosolic fraction of cells using quantitative dot blotting assay : application of selected VH to histochemistry"Journal of Immunological Methods. 261. 65-72 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shin-ichi Takemura: "A mutated superantigen SEA D227A fusion diabody specific to MUC1 and CD3 in targeted cancer immunotherapy for bile duct carcinoma"Cancer Immunology Immunotherapy. 51. 33-44 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Michihiro Nakamura: "Detection of Biotinylated Proteins in Polyacrylamide Gels Using an Avidin-Fluorescein Conjugate"Analytical Biochemistry. 304. 231-235 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] ARUTO UENO: "T-cell Immunotherapy for Human ME-1-Expressing Tumors Using a Fusion Protein of the Super antigen SEA and Anti-MK-1 scFv Antibody"ANTICANCER RESEARCH. 22. 769-776 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] MASAHIRO KAWAHARA: "Selection of Highly Productive Mammalian Cells Based on an Inducible Growth Advantage Using an Antibody/Receptor Chimera"JOURNAL OF BIOSCIENCE AND BIOENGINEERING. 93. 399-404 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Michihiro Nakamura: "The effect of an agglutogen on virus infection : biotinylated filamentous phages and avidin as a model"FEBS Letters. 520. 77-80 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hideki Watanabe: "Selection of human antibody fragments on the basis of stabilization of the variable domain in the presence of target antigens"Biochemical and Biophysical Research Communications. 295. 31-36 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shinobu Honzawa: "Chiral Recognition in the Binding of Helicenediamine to Double Strand DNA : Interactions Between Low Molecular Weight Helical Compounds and a Helical Polymer"Bioorganic & Medicinal Chemistry. 10. 3213-3218 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kouhei Tsumoto: "Inhibition of hepatitis C virus N53 protease by peptides derived from complementarity-determining regions (CDRs) of the monoclonal antibody 8D4:tolerance of a CDR peptide to conformational changes of a target"FEBS Letters. 525. 77-82 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ryutaro Asano: "Antitumor activity of Interleukin-21 prepared by novel refolding procedure from inclusion bodies expressed in Escherichia coli"FEBS Letters. 528. 70-76 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ryutaro Asano: "Efficient construction of a diabody using a refolding system : anti-carcinoembryonic antigen recombinant antibody fragment"Journal of Biochemistry. 13. 903-909 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kouhei Tsumoto: "Selection and Application of novel biomolecules using phage-display"BIOINDUSTRY. 18. 26-33 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kouhei Tsumoto: "Renaturation of Aggregated Proteins"Protein, Nucleic Acids, Enzymes. 46. 1238-1246 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Toshio Kudo: "Review-Second generation bispecific antibodies : Application in cancer immunotherapy"Recent Research Developments in Cancer. 3. 235-255 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kouhei Tsumoto: "Refolding of Denatured Proteins using Arginine"Jikken-Igaku. 21. 1669-1673 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kouhei Tsumoto: "Mini Review : Practical considerations in refolding proteins from inclusion bodies"Protein Expression and Purification. 28. 1-8 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Akiko Yokota: "The role of hydrogen bonding via interfacial water molecules in antigen-antibody complexation : The HyHEL-10-HEL interaction"The Journal of Biological Chemistry. 278. 5410-5418 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Michihiro Nakamura: "A morphological study of filamentous phage infection of Escherichia coli using biotinylated phages"FEBS Letters. 536. 167-172 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mitsuo Umetsu: "How additives influence the refolding of immunoglobulin-folded proteins in a stepwise dialysis system : Spectroscopic evidence for highly efficient refolding of a single-chain Fv fragment"The Journal of Biological Chemistry. 278. 8979-8987 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Masahiro Kawahara: "Bypassing antibiotic selection positive screening of genetically modified cells with an antigen-dependent proliferation switch"Nucleic Acids Research. 31. e32 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Izumi Kumagai: "Structural consequences of target site-directed functional alteration of an antibody : The case of anti-lysozyme antibody, HyHEL-10"The Journal of Biological Chemistry. 278. 24929-24936 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mitsunori Shiroishi: "Human inhibitory receptors ILT2 and ILT4 compete with CD8 for MHC class I binding and bind preferentially to HLA-G"Proceedings of National Academy of Sciences USA. 100. 8856-8861 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kouhei Tsumoto: "Immobilized Protein Disulfide Isomerase As an Additive for Refolding of Inclusion Bodies : Application to Antibody Fragments."Protein Engineering. 16. 535-541 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hiroshi Ueda: "An optimized homogeneous noncompetitive immunoassay based on the antigen-driven enzymatic complementation"Journal of Immunological Methods. 279. 209-218 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mitsuhiro Matsumoto: "On column refolding and characterization of soluble human interleukin-15 receptor α chain produced in Escherichia coli"Protein Expression and Purification. 31. 64-71 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Koji IIDA: "Stable Expression of the Motor Protein Prestin in Chinese Hamster Ovary Cells"JSME International Journal Series C. 46. 1266-1274 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshikazu Tanaka: "Structural Implications for heavy metal-induced reversible assembly and aggregation of a protein : Crystal structure and metal binding properties of Pyrococcus horikoshi CutA"FEBS Letters. 556. 167-174 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mitsuo Umetsu: "Structural characteristics and refolding of in vivo aggregates of archaeon proteins"FEBS Letters. 557. 49-56 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hiroki Hayashi: "An Anti-Epidermal Growth Factor Receptor (EGFR)xAnti-CD3 Bispecific Diabody for Targeting T-LAK Cells to Bile Duct Carcinoma Cells"Cancer Immunology Immunotherapy. 53. 495-507 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Masahiro Kawahara: "AMEGA : antigen-mediated genetically modified cell amplification"Journal of Immunological Methods. 284. 187-194 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Akira Okazaki: "Fucose Depletion from Human IgGI Oligosaccharide Enhances Binding Enthalpy and Association Rate between IgGi and FcγRIIIa"Journal of Molecular Biology. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoko Nagumo: "Phage-Display Selection of Antibodies to the Left End of CTX3C Using Synthetic Fragments"The Journal of Immunological Methods. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshikazu Tanaka: "Crystal Structure of a Hyperthermophilic CutA from Pyrococcus horikoshii in 3 M Guanidine Hydrochloride : Implication for Guanidine -Side Chain Interactions"Biochemical and Biophysical Research Communications. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ryutaro Asano: "Recombinant Antibodies : Present and Perspectives"BIO INDUSTRY. 20. 6-14 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kouhei Tsumoto: "Renaturation of Aggregated Proteins Aggregation Suppression and Refolding"BIO Venture. 32-35 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tsutomu Arakawa: "Magic Agent for Protein Refolding : Arginine"Proteins, Nucleic Acids, Enzymes. 48. 2310-2317 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mitsuo Umetsu: "Effective Renaturation of Aggregated Protein Meets Spectroscopic Studies of Protein Folding"Seibutsu-butsuri. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Izumi Kumagai: "New Protocols for Protein Science, Vol.3(1) Structural and Functional Analyses (T.Ohshima et al. Ed.)"Tokyo Kagaku-Dojin. 200 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kouhei Tsumoto: "Recent Technologies for Utilization of Enzymes (M.Aizawa Ed.)"CMC. 500 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kouhei Tsumoto: "New Frontiers for Combinatorial Biosciences (M.Ueda Ed.)"CMC. 500 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kouhei Tsumoto: "Frontiers for Chemistry Vol.8: Combinatorial Bioengineering (M.Ueda Ed.)"Kagaku-Dojin. 180 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Izumi Kumagai: "Biophysics Handbook"Asakura Shoten(in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kouhei Tsumoto: "New Frontiers for Genomics and Proteomics-Analyses and Application of Bioinformatics-"NTS(in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kouhei Tsumoto: "Molecular Design of Bio-nano machines (Kidokoro Ed.)"Kyoritsu Shuppan. 200 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kouhei Tsumot, et al.: "Mini Review : Practical considerations in refolding proteins from inclusion bodies"Protein Expression and Purification. 28. 1-8 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Akiko Yokota, et al.: "The role of hydrogen bonding via interfacial water molecules in antigen-antibody complexation : The HyHEL-10-HEL interaction"The Journal of Biological Chemistry. 278. 5410-5418 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Michihiro Nakamura, et al.: "A morphological study of filamentous phage infection of Escherichia coil using biotinylated phages"FEBS Letters. 536. 167-172 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mitsuo Umetsu, et al.: "How additives influence the refolding of immunoglobulin-folded proteins in a stepwise dialysis system"The Journal of Biological Chemistry. 278. 8979-8987 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Masahiro Kawahara, et al.: "Bypassing antibiotic selection : positive screening of genetically modified cells with an antigen-dependent proliferation switch"Nucleic Acids Research. 31. e32 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Izumi Kumagai, et al.: "Structural consequences of target site-directed functional alteration of an antibody : The case of anti-lysozyme antibody, HyHEL-10"The Journal of Biological Chemistry. 278. 24929-24936 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mitsunori Shiroishi, et al.: "Human inhibitory receptors ILT2 and ILT4 compete with CD8 for MHC class I binding and bind preferentially to HLA-G"Proc.Natl.Acad.Sci.USA. 100. 8856-8861 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kouhei Tsumoto, et al.: "Immobilized Protein Disulfide Isomerase As an Additive for Refolding of Inclusion Bodies : Application to Antibody Fragments"Protein Engineering. 16. 535-541 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hiroshi Ueda, et al.: "An optimized homogeneous noncompetitive immunoassay based on the antigen-driven enzymatic complementation"Journal of Immunological Methods. 279. 209-218 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mitsuhiro Matsumoto, et al.: "On column refolding and characterization of soluble human interleukin-15 receptor α chain produced in Escherichia coil"Protein Expression and Purification. 31. 64-71 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Koji Iida, et al.: "Stable Expression of the Motor Protein Prestin in Chinese Hamster Ovary Cells"JSME International Journal Series C. 46. 1266-1274 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yoshikazu Tanaka, et al.: "Structural Implications for heavy metal-induced reversible assembly and aggregation of a protein : Crystal structure and metal binding properties of Pyrococcus horikoshii CutA"FEBS Letters. 556. 167-174 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Misuo Umetsu, et al.: "Structural characteristics and refolding of in vivo aggregates of archaeon proteins"FEBS Letters. 557. 49-56 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hiroki Hayashi, et al.: "A highly effective and stable bispecific diabody for cancer immunotherapy : cure of xenografted tumors by bispecific diabody and T-LAK cells"Cancer Immunol Immunother. 53. 495-507 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Masahiro Kawahara, et al.: "AMEGA : antigen-mediated genetically modified cell amplification"Journal of Immunological Methods. 284. 187-194 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Akira Okazaki, et al.: "Fucose Depletion from Human IgG1 Oligosaccharide : Enhances Binding Enthalpy and Association Rate between IgG1 and FcγRIIIa"Journal of Molecular Biology. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yoko Nagumo, et al.: "Phage-Display Selection of Antibodies to the Left End of CTX3C Using Synthetic Fragments"Journal of Immunological Methods. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yoshikazu Tanaka, et al.: "Crystal Structure of a Hyperthermophilic CutA from Pyrococcus horikoshii in 3 M Guanidine Hydrochloride : Implication for Guanidine-Side Chain Interactions"Journal of Molecular Biology. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 浅野竜太郎: "組換え型抗体の現状と将来"Bioindustry. 20. 6-14 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 津本浩平, 熊谷泉: "凝集タンパク質の再生〜凝集抑制と巻き戻し〜"BIOベンチャー. 32-35 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 荒川力: "タンパク質リフォールディングの救世主:アルギニン"蛋白質核酸酵素. 48. 2310-2317 (2003)

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      2003 Annual Research Report
  • [Publications] 梅津光央: "凝集蛋白質の効率的再生と分光学的フォールディング研究の接点"生物物理. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 津本浩平, 熊谷 泉(分担執筆): "化学フロンティア(8) コンビナトリアル・バイオエンジニアリング"化学同人. 180 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 熊谷泉, 津本浩平(分担執筆): "生物物理学ハンドブック"朝倉書店(印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 津本浩平, 熊谷泉(分担執筆): "ゲノミクス・プロテオミクスの新展開〜生物情報の解析と応用〜"エヌ・ティー・エス(印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Michihiro Nakamura, et al.: "Phage library panning against cytosolic fraction of cells using quantitative dot blotting assay : application of selected VH to histochemistry"Journal of Immunological Methods. 261. 65-72 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shin-ichi Takemura, et al.: "A mutated superantigen SEA D227A fusion diabody specific to MUC1 and CD3 in targeted cancer immunotherapy for bile duct carcinoma"Cancer Immunology Immunotherapy. 51. 33-44 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Michihiro Nakamura, et al.: "Detection of Biotinylated Proteins in Polyacrylamide Gels Using an Avidin-Fluorescein Conjugate"Analytical Biochemistry. 304. 231-235 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] ARUTO UENO, et al.: "T-cell Immunotherapy for Human MK-1-Expressing Thmors Using a Fusion Protein of the Superantigen SEA and Anti-MK-1 scFv Antibody"ANTICANCER RESEARCH. 22. 769-776 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] MASAHIRO KAWAHARA, et al.: "Selection of Highly Productive Mammalian Cells Based on an Inducible Growth Advantage Using an Antibody/Receptor Chimera"JOURNAL OF BIOSCIENCE AND BIOENGINEERING. 93. 399-404 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Michihiro Nakamura, et al.: "The effect of an agglutogen on virus infection : biotinylated filamentous phages and avidin as a model"FEBS Letters. 520. 77-80 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hideki Watanabe, et al.: "Selection of human antibody fragments on the basis of stabilization of the variable domain in the presence of target antigens"Biochemical and Biophysical Research Communications. 295. 31-36 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shinobu Honzawa, et al.: "Chiral Recognition in the Binding of Helicenediamine to Double Strand DNA : Interactions Between Low Molecular Weight Helical Compounds and a Helical Polymer"Bioorganic & Medicinal Chemistry. 10. 3213-3218 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kouhei Tsumoto, et al.: "Inhibition of hepatitis C virus NS3 protease by peptides derived from complementarity-determining regions (CDRs) of the monoclonal antibody 8D4"FEBS Letters. 520. 77-82 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shusaku Tsumoto, et al.: "Analysis of amino-acid sequences by statistical technique"Information Sciences. 145. 205-214 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ryutaro Asano, et al.: "Antitumor activity of Interleukin-21 prepared by novel refolding procedure from inclusion bodies expressed in Escherichia coli"FEBS Letters. 528. 70-76 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ryutaro Asano, et al.: "Efficient construction of a diabody using a refolding system : anti-carcinoembryonic antigen recombinant antibody fragment"Journal of Biochemistry. 123. 903-909 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kouhei Tsumoto, et al.: "Mini Review : Practical considerations in refolding proteins from inclusion bodies"Protein Expression and Purification. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Akiko Yokota, et al.: "The role of hydrogen bonding via interfacial water molecules in antigen-antibody complexation : The HyHEL-10-HEL interaction"The Journal of Biological Chemistry. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Michihiro Nakamura, et al.: "A morphological study of filamentous phage infection of Escherichia coli using biotinylated phages"FEBS Letters. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mitsuo Umetsu, et al.: "How additives influence the refolding of immunoglobulin-folded proteins in a stepwise dialysis system : Spectroscopic evidence for highly efficient refolding of a single-chain"The Journal of Biological Chemistry. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Masahiro Kawahara, et al.: "Bypassing antibiotic selection : positive screening of genetically modified cells with an antigen-dependent proliferation switch"Nucleic Acids Research. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Izumi Kumagai, et al.: "Structural consequences of target site-directed functional alteration of an antibody : The case of anti-lysozyme antibody, HyHEL-lO"The Journal of Biological Chemistry. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 津本, 熊谷(分担): "コンビナトリアルサイエンスの新展開"シーエムシー出版. 400 (2002)

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      2002 Annual Research Report
  • [Publications] 津本, 熊谷(分担): "新タンパク質科学実験法 第3巻構造機能解析(上)"東京化学同人. 200 (2003)

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      2002 Annual Research Report
  • [Publications] 津本, 熊谷(分担): "化学フロンティア(8) コンビナトリアル・バイオエンジニアリング"化学同人. 200 (2003)

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      2002 Annual Research Report
  • [Publications] Yoda et al.: "Folding-unfolding of goat alpha-lactalbumin studied by stopped-flow CD and molecular dynamic simulations"Proteins ; Struct. Funct. Genet.. 42. 49-65 (2001)

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      2001 Annual Research Report
  • [Publications] Arai et al.: "Demonstration of a homogeneous noncompetitive immunoassay based on bioluminescence resonance energy transfer"Analytical Biochemistry. 289. 77-81 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nakamura et al.: "Panning of phage VH library using nitrocellulose membranes-application to selection of human VH library"The Journal of Biochemistry. 129. 209-212 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kasai et al.: "Fv fragment of an antibody specific for hepatitis C NS3 protease domain : The sequence analysis, bacterial expression and functional characterization"Biochem. Biophys. Res. Commun. 281. 416-424 (2001)

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      2001 Annual Research Report
  • [Publications] Ueno et al.: "T-cell Immunotherapy for Human MK-1-Expressing Tumors Using a Fusion Protein of the Superantigen SEA and Anti-MK-1 scFv Antibody"Anticancer research. 22 印刷中. (2002)

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      2001 Annual Research Report
  • [Publications] 津本: "不溶性顆粒からの蛋白質の巻き戻し"蛋白質核酸酵素. 46. 1238-1246 (2001)

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      2001 Annual Research Report
  • [Publications] 津本, 熊谷: "ファージディスプレーを用いた新規バイオ分子の選択と応用"BIOINDUSTRY. 18. 26-33 (2001)

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      2001 Annual Research Report
  • [Publications] Nakamura et al.: "Detection of biotinylated proteins in polyacrylamide gels using an avidin-fluorescein conjugate"Analytical Biochemistry. 印刷中. (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shiroishi et al.: "Structural evidence for entropic contribution of salt bridge formation to a protein antigen-antibody interaction : The case of HyHEL-10 Fv-hen lysozyme complex"The Journal of Biological Chemistry. 276. 23042-23050 (2001)

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      2001 Annual Research Report
  • [Publications] Kawahara et al.: "Replacing factor dependency with that for lysozyme : Affordable culture of IL-6 dependent hybridoma by transfecting artificial cell surface receptor"Biotechnology and Bioengineering. 76. 416-423 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nakamura et al.: "The effect of an agglutogen on virus infection : biotinylated filamentous phages and avidin as a model"FEBS Letters. 印刷中. (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nakamura et al.: "Selection of highly productive mammalian cells based on an inducible growth advantage using an antibody/receptor chimera"J. Biosci. Biotechnol.. 印刷中. (2002)

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      2001 Annual Research Report
  • [Publications] Nakamura et al.: "A growth signal with an artificially induced erythropoietion receptor-gp 130 cytoplasmic domain heterodimer"The Journal of Biochemistry. 130. 305-312 (2001)

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      2001 Annual Research Report
  • [Publications] Horii et al.: "Contribution of Thr29 to the Thermodynamic Stability of Goat α-Lactalbumin As Determined by Experimental and Theoretical Approaches"PROTEINS : Struct. Funct. Genet.. 45. 16-29 (2001)

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  • [Publications] Nakamura et al.: "A visualization method of filamentous phage infection and phage-derived proteins in Escherichia coli using biotinylated phages"Biochem. Biophys. Res. Commun.. 289. 252-256 (2001)

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  • [Publications] Kodama et al.: "Mutated SEA D227A-conjugated antibodies greatly enhance antitumor activity against MUC1-expressing bile duct carcinoma (BDC)"Cancer Immunology Immuntherapy. 50. 539-548 (2001)

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  • [Publications] Nakamura et al.: "Phage library panning against cytosolic fraction of cells using quantitative dot blotting : Application of selected VH to histochemistry"Journal of Immunological Methods. 261. 65-72 (2002)

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  • [Publications] Takemura et al.: "A mutated superantigen SEA D227A fusion diabody specific to MUC1 and CD3 for targeted cancer immunotherapy for bile duct carcinoma"Cancer Immunology Immunotherapy. 印刷中. (2002)

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  • [Publications] Kumagai, Tsumoto: "Encyclopedia of Life Sciences"Blackwell 印刷中. (2002)

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  • [Publications] 津本, 熊谷: "コンビナトリアルバイオエンジニアリング"化学同人 印刷中. (2002)

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  • [Publications] 津本, 熊谷: "新タンパク質科学実験法 第3巻構造機能解析"東京化学同人 印刷中. (2002)

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Published: 2001-04-01   Modified: 2016-04-21  

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