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Chemical Speciation of Trace Metallic Elements in Biological and Environmental Samples

Research Project

Project/Area Number 13450346
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 工業分析化学
Research InstitutionNagoya University

Principal Investigator

HARAGUCHI Hiroki  Nagoya University, Graduate School of Engineering, Department of Applied Chemistry, Professor, 大学院・工学研究科, 教授 (70114618)

Co-Investigator(Kenkyū-buntansha) FUJIMORI Eiji  Nagoya University, School of Engineering, Department of Applied Chemistry, Research Associate, 大学院・工学研究科, 助手 (30291412)
ITOH Akihide  Nagoya University, Graduate School of Engineering, Department of Applied Chemistry, Lecturer, 大学院・工学研究科, 講師 (60273265)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥10,800,000 (Direct Cost: ¥10,800,000)
Fiscal Year 2002: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2001: ¥7,500,000 (Direct Cost: ¥7,500,000)
KeywordsBiological samples / Environmental samples / Trace metallic elements / Chemical speciation / Bile-acid coated column / Size exclusion chromatography / Hyphenated system / ICP-MS / HPLC / ICP-AES / 微量金属 / 血清 / 天然水 / 界面活性剤被覆ODSカラム / ポリアクリルアミド電気泳動
Research Abstract

In the present research, chemical speciation of trace metallic elements in the biological and environmental samples has been carried out. So far, in the studies concerning with trace metals, total analysis of trace metals has been mostly performed because of their too low concentrations. However, these days, it is well known that physiological functions and/or toxicities of metallic elements in the biological and environmental samples depend on their chemical forms. Thus, development of the analytical methods for chemical speciation, in which chemical species of each element would be elucidated, is very much expected in the various scientific fields. In our group, a hyphenated system of SEC (size exclusion chromatography) /ICP-MS, in which the column coated with one of bile-acid derivatives (CHAPS ; 3-[3-cholamidopropyl]-dimethylammonio)propanesulfonic acid) was used as the stationary phase, was developed, and that system was applied to chemical speciation analysis of human blood serum, salmon egg cell, tea leaves, seawater, lake water and so forth. The following studies have been mainly carried out ; (1) speciation of cisplatin [cis-diammaninedichloroplatinum(II)] in human blood serum, which is a anti-tumor drug used in clinical treatment, (2) multielement determination of major-to-ultratrace elements in salmon egg cell and speciation of small molecules and ions in cytoplasm, (3) elucidation of the dissolved states of trace metals in lake water, especially, in terms of the formation of large organic molecule-meta complexes (LOMMCs).

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] H.Matsuura, A.Hokura, F.Katsuki, A.Itoh, H.Haraguchi: "Multielement Determination and Speciation of Major-to-Trace Elements in Black Tea Leaves by ICP-AES and ICP-MS with Aid of Size Exclusion"Analytical Sciences. 17・3. 391-398 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 原口紘き, 大蔦友美, 松浦博孝, 長谷川拓也: "界面活性剤被覆固定相を用いたHPLC/ICP-MSによる血清中生体微量元素のスペシエーション及び薬剤分析"Biomedical Research on Trace Elements. 12・2. 128-140 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Matsuura, H.Haraguchi: "Multielement Determination of Major-to-Ultratrace Elements in Salmon Egg by ICP-AES and ICP-MS"Analytical Sciences. 17. i975-i978 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Haraguchi, T.Oshima, H.Matsuura, T.Hasegawa: "Speciation of Drugs in Blood Serum by Surfactant-Mediated HPLC/JCP-MS with Direct Sample Injection"Analytical Sciences. 17. i37-i40 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 原口紘き, 長谷川拓也: "地球システムの全元素化学と拡張元素普存説"Biomedical Research on Trace Elements. 13・1. 31-45 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Matsuura, T.Hasegawa, H.Nagata, K.Takatani, M.Asano, A.Itoh, H.Haraguchi: "Speciation of Small Molecules and Inorganic Ions in Salmon Egg Cell Cytoplasm by Surfactant-mediated HPLC/ICP-MS"Analytical Sciences. 19・1. 117-121 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 原口紘き(分担執筆): "機器分析実験"梅沢喜夫, 水本昌一, 渡会仁, 寺前紀夫編;東京化学同人. 387 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Matsuura, A.Hokura, F.Katsuki, A.Itoh, H.Haraguchi: "Multielement Determination and Speciation of Major-to-Trace Elements in Blck Tea Leaves by ICP-AES and ICP-MS with Aid of Size Exclusion Chromatography"Analytical Sciences. 17-3. 391-398 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Haraguchi, T.Ohshima, H.Matsuura, T.Hasegawa: "Speciation and Drug Analysis of Bio-trace Elements in Human Blood Serum by HPLC/ICP-MS Using Surfactant-Mediated Stationary Column"Biomedical Research on Trace Elements. 12-2. 128-140 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Matsuura, H.Haraguchi: "Multielement Determination of Major-to-Ultratrace Elements in Salmon Egg by ICP-AES and ICP-MS"Analytical Sciences. 17(Supplement). i975-i978 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Haraguchi, T.Ohshima, H.Matsuura, T.Hasegawa: "Speciation of Drugs in Blood Serum by Surfactant-Mediated HPLC/ICP-MS with Direct Sample Injection"Analytical Sciences. 17(Supplement). i37-i40 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Hasegawa, K.Inagaki, H.Haraguchi: "Correlation Analysis of Major-to-Trace Elementsin Human Blood Serum for Medical Diagnosis as Studied by ICP-AES and ICP-MS"Analytical Sciences. 17(Supplement). i979-i982 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Haraguchi, T.Hasegawa: "All-Elements Chemistry of the Earth System and Extended All-Present Theory of the Elements"Biomedical Research on Trace Elements. 13-1. 31-45 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Matsuura, T.Hasegawa, H.Nagata, K.Takatani, M.Asano, A.Itoh, H.Haraguchi: "Speciation of Small Molecules and Inorganic Ions in Salmon Egg Cell Cytoplasm by Surfactant-Mediated HPLC/ICP-MS"Analytical Sciences. 19-1. 117-121 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Hasegawa, H.Matsuura, K.Inagaki, H.Haraguchi: "Major-to-Ultratrace Elements in Bone Marrow Fluid as Determined by ICP-AES and ICP-MS"Analytical Sciences. 19-1. 147-150 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Matsuura, A.Hokura, F.Katsuki, A.Itoh, H.Haraguchi: "Multielement Determination and Speciation of Major-to-Trace Elements in Black Tea Leaves by ICP-AES and ICP-MS with Aid of Size Exclusion"Analytical Sciences. 17・3. 391-398 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 原口紘き, 大嶌友美, 松浦博孝, 長谷川拓也: "界面活性剤被覆固定相を用いたHPLC/ICP-MSによる血清中生体微量元素のスペシエーション及び薬剤分析"Biomedical Research on Trace Elements. 12・2. 128-140 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Matsuura, H.Haraguchi: "Multielement Determination of Major-to-Ultratrace Elements in Salmon Egg by ICP-AES and ICP-MS"Analytical Sciences. 17. i975-i987 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Haraguchi, T.Oshima, H.Matsuura, T.Hasegawa: "Speciation of Drugs in Blood Serum by Surfactant-Mediated HPLC/ICP-MS with Direct Sample Injection"Analytical Sciences. 17. i37-i40 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 原口紘き, 長谷川拓也: "地球システムの全元素化学と拡張元素普存説"Biomedical Research on Trace Elements. 13・1. 31-45 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Matsuura, T.Hasegawa, H.Nagata, K.Takatani, M.Asano, A.Itoh, H.Haraguchi: "Speciation of Small Molecules and Inorganic Ions in Salmon Egg cell Cytoplasm by Surfactant-mediated HPLC/ICP-MS"Analytical Sciences. 19・1. 117-121 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 原口紘き(分担執筆): "機器分析実験"梅沢喜夫, 水本昌二, 渡会 仁, 寺前紀夫編;東京化学同人. 387 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 岩田聖子, 皆本和亮, 藤森英治, 千葉光一, 原口紘〓: "産業廃棄物焼却飛灰標準試料の作成と評価"分析化学. 50(12). 837-844 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] E. Fujimori, K. Ichikawa, R. Shiozawa, K. Asai, K. Chiba, H.Haraguchi: "Multielement Determination and Distributions of Major-to-Ultratrace Elements in Industrial Waste Incineration Bottom Ash as Studied by ICP-AES and ICP-MS"The Bulletin of Chemical Society of Japan. 74(11). 2045-2051 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 境 剛史, 中川光司, 紀 杉, 伊藤彰英, 原口紘〓: "深層海水塩の多元素定量分析"Biomedical Research on Trace Elements. 12(4). 239-240 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 山田 浩, 紀 杉, 伊藤彰英, 千葉光一, 原口紘〓: "キレート樹脂濃縮を併用した多元素同位体希釈/ICP-MSによる河川水中Cu, Cd, Pbの定量"分析化学. 50(6). 433-439 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 岩田聖子, 藤森英治, 千葉光一, 原口紘〓: "ICP-MSによる産業廃棄物焼却飛灰中希土類元素の定量と希土パターンの特徴"分析化学. 50(6). 419-425 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 原口 紘〓(分担執筆): "機器分析実験"東京化学同人. 6 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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