| Project/Area Number |
13460009
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
作物学
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| Research Institution | Kobe University |
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Principal Investigator |
UCHIDA Naotsugu Kobe University, Faculty of Agriculture, Professor, 農学部, 教授 (70151884)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIMOTO Toshio Kobe University, Faculty of Agriculture, Professor, 農学部, 教授 (70240851)
ISHII Takashige Kobe University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (20260648)
AZUMA Tetsushi Kobe University, Graduate School of Science and Technology, Associate Professor, 大学院・自然科学研究科, 助教授 (30231913)
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| Project Period (FY) |
2001 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥15,500,000 (Direct Cost: ¥15,500,000)
Fiscal Year 2004: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2002: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2001: ¥8,700,000 (Direct Cost: ¥8,700,000)
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| Keywords | Rice / Photosynthesis / Yield / Wild species / Genome / Rubisco |
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| Research Abstract |
The present study was carried out to get some information for using wild rice species to improve leaf photosynthesis and grain yield. We examined photosynthetic rates and properties of dry matter production in BC2F1-BC2F4 lines.
In BC2F1 populations, several lines had significantly higher photosynthetic rates (OER) than parental cultivars, and 14-25% of plants in the populations had higher OERs than the highest values in parental cultivars. These higher OERs showed positive correlation with the contents of Rubisco and/or Rubisco activase. Four lines of BC2F4 were generated by backcrossing wild rice with cultivar Nipponbare. The photosynthetic rates under saturated light and ambient air conditions in these 4 lines were average 7% higher than Nipponbare. Also, the grain yield per plant of BC2F4 lines were average 16% higher These results suggest that O.rufipogon can be used as a source of germplasm to improve the photosynthetic capacity and grain yield of O.sativa.
In order to identify useful QTL alleles from wild rice relatives, we backcrossed with two typical rice cultivars, japonica Nipponbare and indica IR36. In the BC2 generation, several characters including photosynthetic activity were evaluated, and single-point QTL analysis was carried out using 74 microsatellite markers. From these analysis, two wild QTL alleles increasing photosynthetic activity was detected. The present results suggest that the wild QTL alleles are very useful in improving photosynthetic function and yield of rice.
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