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Functions of water channel in the growth and development of horticultural crops.

Research Project

Project/Area Number 13460012
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 園芸・造園学
Research InstitutionNagoya University, Graduation School of Bioagricultural Sciences

Principal Investigator

SHIRATAKE Katsuhiro  Nagoya University, Graduation School of Bioagricultural Sciences, Associate Professor, 大学院・生命農学研究科, 助教授 (90303586)

Co-Investigator(Kenkyū-buntansha) MAESHIMA Masayoshi  Nagoya University, Graduation School of Bioagricultural Sciences, Professor, 大学院・生命農学研究科, 教授 (80181577)
YAMAKI Shohei  Nagoya University, Graduation School of Bioagricultural Sciences, Professor, 大学院・生命農学研究科, 教授 (70210341)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 2003: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥3,600,000 (Direct Cost: ¥3,600,000)
Keywordswater channel / aquaporin / water transport / horticultural crop / plant growth / plasma membrane / vacuolar membrane / tonoplast / セイヨウナシ / ダイコン / アサガオ / リン酸化 / ストレス応答 / 果実生長 / 開花 / 水輪送活性 / 水チャンネル / 細胞肥大
Research Abstract

1, Water Channel in Fruit
(1)Protein level of tonoplast water channel (TIP) of pear was high in young fruit, on the other hand, that of TIP and plasma membrane water channel (PIP) of grape berry was the almost constant during development. This suggests that water channel a play role in water regulation rather than fruit development.
(2)Gene expression of TIP was high in young pear fruit, but that of PIP showed almost constant level during development. Gene expressions of TIP and PIP in fruit was especially higher than those in other organs, suggesting the importance of water channel in fruit.
(3)Phosphorylation of PIP2 was determined using the antibody which could recognize only phosphorylated PIP2. PIP2 was de-phospholylared by salt and osmotic stresses and also in the fruit in day time.
2, Water Channel in Radish
(1)Protein and gene expressions of TIP and PIP were high in developing tissue or vascular tissue, such as central cylinder in young root and hypocotyl, and bundle sheath in petiole. This meets physiological function.
(2)Water transport of the membrane from yeast expressed each TIP or PIP was measured. TIP and PIP2 transported water. On the other hand, PIPI did not transport water and it might play a role other than water transport.
(3)By the treatment of osmotic stress or plant hormone to radish seedling, mRNA and protein levels of TIP and PIPL did not change, but those of PIP2 did. These results show that TIP and PIP are housekeeping type and PIP2 is environmentally inducible one.
(4)The epidermis cells of hypocotyl and the parenchyma cells of taproot of radish were expressed water channels at high levels and their water transport activities were also high (300-500 mm/sec).

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (42 results)

All Other

All Publications (42 results)

  • [Publications] Shiratake, K.: "Fluctuations of the protein levels of H^+-pumps and water channels of tonoplast and plasma membrane during grape berry development."J.Japan.Soc.Hort.Sci.. 70. 287-293 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shiratake, K.: "Molecular cloning of tonoplast water channel of pear fruit and its expression with the development."J.Japan.Soc.Hort.Sci.. 70. 281-286 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Frangne, N.: "Expression and distribution of a vacuolar aquaporin in young and mature leaf tissues of Brassica napus in relation to water fluxes."Planta. 212. 270-278 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suga, S.: "Specificity of the accumulation of mRNAs and proteins of the plasma membrane and tonoplast aquaporins in radish organs."Plant. 212. 294-304 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohshima, Y.: "Low aquaporin content and low osmotic water permeability of the plasma and vacuolar membranes of a CAM plant Graptopetalum paraguayense : comparison with radish."Plant Cell Physiol.. 42. 1119-1129 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suga, S.: "Aquaporin isoforms responsive salt and Water stresses and phytohormones in radish seedlings."Plant Cell Physiol.. 43. 1229-1237 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yang, S.: "Modulation of vacuolar H^+-pumps and aquaporin by phytohormones in rice seedling leaf sheaths."Biol.Pharm.Bull.. 26. 88-92 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suga, S.: "Differences in aquaporin levels among cell types of radish and measurement of osmotic water permeability of individual protoplasts."Plant Cell Physiol.. 44. 277-286 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 須賀しのぶ: "植物における水の輸送とアクアポリン"植物の生長調節. 38. 220-228 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 前島正義: "ノーベル化学賞のスポットライトをあびたアクアポリン"化学と生物. 42. 41-42 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sazuka, T.: "Identification of membrane-bound proteins from a vacuolar membrane-enriched fraction of Arabidopsis thaliana"DNA Research.. (In press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 前島正義: "朝倉植物生理学講座 第2巻 代謝「第4章水代謝」分担"朝倉書店. 8 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 白武 勝裕: "細胞工学別冊 植物細胞工学 18 植物の膜輸送システム「シロイヌナズナのゲノムからみた膜輸送系」分担"秀潤社. 3 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 白武 勝裕: "細胞工学別冊 植物細胞工学 18 植物の膜輸送システム「シロイヌナズナ膜輸送系の分類と主な既知膜輸送系との対応」分担"秀潤社. 8 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shiratake, K.: "Fluctuations of the protein levels of H^+-pumps and water channels of tonoplast and plasma membrane during grape berry development."J.Japan.Soc.Hort.Sci.. 70. 87-293 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shiratake, K.: "Molecular cloning of tonoplast water channel of pear fruit arid its expression with the development."J.Japan.Soc.Hort.Sci.. 70. 1-286 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Frarigne, N.: "Expression and distribution of a vacuolar aquaporin in young and mature leaf tissues of Brassica napus in relation to water fluxes."Planta. 212. 270-278 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suga, S.: "Specificity of the accumulation of mRNAs and proteins of the plasma membrane and tonoplast aquaporins in radish organs."Planta. 212. 294-304 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohshima, Y.: "Low aquaporin content and low osmotic water permeability of the plasma and vacuolar membranes of a CAM plant Graptopetalum paraguayense : comparison with radish."Plant Cell Physiol.. 42. 1119-1129 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suga, S.: "Aquaporin isoforms responsive to salt and water stresses and phytohormones in radish seedlings."Plant Cell Physiol.. 43. 1229-1237 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yang, S.: "Modulation of vacuolar H^+-pumps and aquaporin by phytohormones in rice seedling leaf sheaths."Biol.Pharm.Bull.. 26. 88-92 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suga, S.: "Differences in aquaporin levels among cell types of radish and measurement of osmotic water permeability of individual protoplasts."Plant Cell Physiol.. 44. 277-286 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suga, S.: "Water transport and aquaporin in plant."Syokubutsu-no Seityou Tyousetsu(in Japanese). 38. 220-228 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Maeshima, M.: "Spotlighted aquaporin in Nobel Prise."Kagaka-to Seibutsu(in Japanese). 42. 41-42 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sazuka, T.: "Identification of membrane-bound proteins from a vacuolar membrane-enriched fraction of Arabidopsis thaliana."DNA Research. (In press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suga, S.: "An abundance of aquaporins in specific cells and osmotic water permeability of individual protoplasts of radish."Plant Cell Physiol.. 44. 277-286 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 白武 勝裕: "シロイヌナズナのゲノムからみた膜輸送系"細胞工学別冊 植物細胞工学. 18. 18-20 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 白武 勝裕: "シロイヌナズナ膜輸送系の分類と主な既知膜輸送系との対応"細胞工学別冊 植物細胞工学. 18. 216-223 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 須賀 しのぶ: "植物における水の輸送とアクアポリン"植物の生長調節. 38. 220-228 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 前島 正義: "ノーベル化学賞のスポットライトをあびたアクアポリン"化学と生物. 42. 41-42 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Suga, S.: "Aquaporin isoforms responsive to salt and water stresses and phytohormones in radish seedlings"Plant Cell Physiol.. 43. 1229-1237 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yang, S.: "Modulation of vacuolar H^+-pumps and aquaporin by phytohormones in rice seedling leaf sheaths"Biol. Pharm. Bull.. 26. 88-92 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Suga, S.: "An abundance of aquaporins in specific cells and osmotic water permeability of individual protoplasts of radish"Plant Cell Physiol. 44(In press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 白武 勝裕: "シロイヌナズナのゲノムからみた膜輸送系"細胞工学別冊 植物細胞工学. 18(印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 白武 勝裕: "シロイヌナズナ膜輸送系の分類と主な既知膜輸送系との対応"細胞工学別冊 植物細胞工学. 18(印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shiratake, K.: "Fluctuations of the protein levels of H^+-pumps and water channels of tonoplast and plasma membrane during grape berry development"J. Japan. Soc. Hort. Sci.. 70. 287-293 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shiratake, K.: "Molecular cloning of tonoplast water channel of pear fruit and its expression with the development"J. Japan. Soc. Hort. Sci.. 70. 281-286 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Frangne, N.: "Expression and distribution of vacuolar aquaporin in young and mature leaf tissues of Brassica napus in relation to water fluxes"Planta. 212. 270-278 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Suga, S.: "Specificity of the accumulation of mRNAs and proteins of the plasma membrane and tonoplast aquaporins in radish organs"Planta. 212. 294-304 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Maeshima, M.: "Tonoplast transporters : organization and function"Annu. Rev. Plant Physiol. Plant Mol. Biol.. 52. 469-497 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ohshima, Y.: "Low aquaporin content and low osmotic water permeability of the plasma and vacuolar membranes of a CAM plant Graptopetalum paraguayense : comparison with radish"Plant and Cell Physiology. 42. 1119-1129 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 前島正義: "朝倉植物生理学講座 第2巻 代謝 (第4章 水代謝 分担)"朝倉書店. 8 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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