| Project/Area Number |
13460014
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
園芸・造園学
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| Research Institution | Kyoto University |
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Principal Investigator |
TAO Ryutaro Kyoto University, Graduate School of Agriculture, Associate Professor, 農学研究科, 助教授 (10211997)
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| Co-Investigator(Kenkyū-buntansha) |
HABU Tsuyoshi Kyoto University, Graduate School of Agriculture, Assistant Professor, 農学研究科, 助手 (60335304)
SASSA Hidenori Yokohama City University, Kihara Institute for Biological Research and Graduate School of Integrated Science, Assistant Professor, 木原生物学研究所, 助手 (50295507)
別府 賢治 香川大学, 農学部, 助手 (30281174)
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| Project Period (FY) |
2001 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥13,300,000 (Direct Cost: ¥13,300,000)
Fiscal Year 2004: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2003: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2002: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2001: ¥4,200,000 (Direct Cost: ¥4,200,000)
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| Keywords | stone fruit / S-RNase / gametophytic self-incompatibility / sweet cherry / Japanese apricot / almond / Japanese plum / peach |
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| Research Abstract |
This study identifies the pollen determinant (pollen S) of the S-RNase based gametophytic self-incompatibility system utilizing the mutated S haplotypes. Based on the results obtained we have developed the working model for the molecular basis of self-(in)compatibility in Prunus. The summary of the results obtained is as follows.
(1)Analysis of the mutated S haplotype in almond
Comparisons of the S locus region of a mutated Sc-hapotype with the original one identifies S haplotype-specific F-box progein gene (SFB). SFB shows the S haplotype-specific sequence polymorphism and pollen-specific expression, indicating that SFB is a very good candidate for the pollen Sin Prunus.
(2)Analysis of self-compatible S haplotypes of cherry and Japanese apricot
Sf of Japanese apricot and S4' of sweet cherry are known to be the pollen part mutant S haplotypes. Analysis of their S locus regions revealed insertion mutations in SFBs of these S haplotypes. The insertions lead to frame shift to produce truncated transcripts for SFB, which could be the cause of self-compatibility in Sf and S4' haplotypes.
(3)Self-(in)compatibility in tetraploid sour cherry
In Solanaceae, which shows the same type of gametophytic self-incompatibility as Prunus, polyploidization generally leads to self-compatibility through the so-called phenomenon, "the competitive interaction". We have analyzed self-(in)compatibility in the progeny of several crosses between several sour cherry cultivars. It appeared that the segregation of self-(in)compatibility is determined by the number of functional and non-functional S haplotypes present in the progeny, suggesting that "the competitive interaction" is absent in Prunus.
(4)Working model for the molecular basis of self-(in)compatibility in Prunus
Based on the above-described results, we have developed and published the working model for the genetic and molecular basis of self-(in)compatibility in Prunus.
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