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Molecular analysis of virulence and avirulence in Xanthomonas orizae pv.oryzae

Research Project

Project/Area Number 13460024
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物保護
Research InstitutionHiroshima Prefectural University

Principal Investigator

OK Takashi  HIROSHIMA PREFECTURAL UNIVERSITY, SCHOOL OF BIORESOURCES, ASSOCIATE PROFESSOR, 生物資源学部, 助教授 (50201992)

Co-Investigator(Kenkyū-buntansha) TSUGE Seiji  KYOTO PREFECTURAL UNIVERSITY, GRADUATE SCHOOL OF AGRICULTURE, LECTURER, 大学院・農学研究科, 講師 (10254319)
TSUNO Kazunori  MIYAZAKI UNIVRSITY, FACULTY OF AGRIGUSTURE, ASSOCIATE PROFESSOR, 農学部, 助教授 (50207429)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥13,000,000 (Direct Cost: ¥13,000,000)
Fiscal Year 2003: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 2002: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 2001: ¥4,600,000 (Direct Cost: ¥4,600,000)
KeywordsXanthomonas oryzae pv.oryzae / hrp / virulence gene / avirulence gene / Type III secretion system / secretion protein / molecular biology / hrpXo / 変異 / Xanthomonas oryzae / 発現培地 / 発現機構
Research Abstract

Same as other Gram-negative plant-pathogenic bacteria clustered hrp genes encoding components of the type III secretion system are indispensable for pathogenicity of Xanthomonas oryzae pv.oryzae, a causal agent of bacterial leaf blight of rice. We investigated a structure of the hrp gene cluster in X.oryzae pv.oryzae by sequence analysis. Comparative studies between two Japanese strains of X.oryzae pv.oryzae (N7501: Japanese race 2 and T7174: Japanese race 1) revealed that the hrp gene cluster was equivalent in both the stractural arrangement and the nucleotide sequence of each gene involved, and that ORFs located in the interspace region between hpaB and hrpF were quite different from those of the other xanthomonads. In general, expression of hrp genes is known to be induced only in plants and some specific synthetic media. We have succeeded in constructing a hrp-inducing medium for X.oryzae pv.oryzae, named XOM2, containing xylose as a sole sugar source. Using this medium, we identified three HrpXo-dependent type III secretory proteins ; HrpE1,HrpF and Hpa1, and a HrpXo-dependent type II secretory protein ; cysteine protease homolog from strain T7174R. Also, to clarify race differentiation of the bacterium based on the molecular structure, regions specific to races in the X.oryzae pv.oryzae genome were analyzed. Sequence analysis revealed that a chromosomal region specific to Japanese race 3 (strain T7133) contained insertion sequences, IS transposase genes and avrBs3 homologs. These sequences might be involved in the race differentiation of Xoryzae pv.oryzae.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] 津下誠治: "Expression of Xanthomonas oryzae pv. oryzae hrp genes in XOM2, a novel synthetic medium."Journal of General Plant Pathology. 68(4). 363-371 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 古谷綾子: "Hpal is secreted via Type III secretion system in Xanthomonas oryzae pv. oryzae."Journal of General Plant Pathology. 69(4). 271-275 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 古谷綾子: "Evidence for hypXo-dependent expression of type II secretory proteins in Xanthomonas oryzae pv. orzae."Journal of Bacteriology. 186(5). 1374-1380 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 奥 尚: "Structural conservation of hrp gene cluster in Xanthomonas oryzae pv. oryzae."Journal of General Plant Pathology. 70(3)(Accepted). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 津下誠治: "Involvement of phosphoglucose isomerase in pathogenicity of Xanthomonas oryzae pv. oryzae."Phytopathology. 94(5). 478-483 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Seiji Tsuge, Ayako Furutani, Rie Fukunaka, Takashi Oku, kazunori Tsuno, Hirokazu Ochiai, Yasuhiro Inoue, Hisatoshi Kaku, Yasuyuki: "Expression of Xanthomonas oryzae pv.oryzae hrp genes in XOM2, a novel synthetic medium"J.Gen.Plant Pathol.. 68(4). 363-371 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ayako furutani, Seiji Tsuge, Takashi Oku, Kazunori Tsuno, Yasuhiro Inoue, Hirokazu Ochiai, Hisatoshi Kaku, Yasuyuki Kubo: "Hpa1 is secreted via Type III secretion system in Xanthomonas oryzae pv.oryzae"J.Gen.Plant Pathol.. 69(4). 271-275.DOI 10.1007/s 10327-003-0042-2 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Furutani, A., tsuge, S., Ohnishi, K., Hikichi, Y., Inoue, Y., Ochiai, H., Oku, T., Tsuno, K., Kaku, H., Kubo, Y.: "Evidence for HrpXo-Dependent Expression of Type II Secretory Proteins in Xanthomonas oryzae pv.oryzae"J.Bacteriol.. 186(5). DOI10.1128/JB.186.5.1374-DOI10.1128/JB.186.5.1380 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Oku, T., Tanaka, K., Iwamoto, M., Inoue, Y., Ochiai, H., Kaku, H., Tsuge, s., Tsuno, K.: "Structural conservation of the hrp gene cluster in Xanthomons oryzae pv.oryzae"J.Gen.Plant Pathol.. 70(3) (in Print). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tsuge, S., Ochiai, H., Inoue, Y., Oku, T., Tsuno, K., Kaku, H., Kubo, Y.: "Involvement of Phosphoglucose Isomerase in Pathogenicity of Xanthomonas oryzae pv.oryzae"Phytopathology. 94(5). 478-483 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 津下誠治: "Expression of Xanthomonas oryzae pv.oryzae hrp genes in XOM2, a novel synthetic medium."Journal of General Plant Pathology. 68(4). 363-371 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] 古谷綾子: "Hpal is secreted via Type III secretion system in Xanthomonas oryzae pv.oryzae."Journal of General Plant Pathology. 69(4). 271-275 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 古谷綾子: "Evidence for hrpXo-dependent expression of type II secretory proteins in Xanthomonas oryzae pv.oryzae."Journal of Bacteriology. 186(5). 1374-1380 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 奥 尚: "Structural conservation of hrp gene cluster in Xanthomonas oryzae pv.oryzae."Journal of General Plant Pathology. 70(3)(Accepted). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 津下誠治: "Involvement of phosphoglucose isomerase in pathogenicity of Xanthomonas oryzae pv.oryzae."Phytopathology. 94(未定)(Accepted). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 津下誠治: "Expression of Xanthomonas oryzae hrp. oryzae hrp genes in XOM2, a novel synthetic medium"Journal of General Plant Pathology. 68(4)(Accepted). (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 古谷綾子: "Hpa1 is secreted via Type III secretion system in Xanthomonas oryzae pv. oryzae"Journal of General Plant Pathology. 69(4)(Accepted). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 古谷綾子: "イネ白葉枯病菌のhpaP変異株のイネ葉内での増殖について"日本植物病理学会報. 67・2. 211 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 奥 尚: "非親和性イネ白葉枯病菌接種イネ品種Te-tepからの防御反応関連mRNAの検出"日本植物病理学会報. 68・1(印刷中). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 福中理恵: "イネ白葉枯病菌のin vitroにおけるhrp発現誘導条件の検討"日本植物病理学会報. 68・1(印刷中). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 古谷綾子: "イネ白葉枯病菌のhpaP遺伝子の病原性への関与およびそのin vitroにおける発現"日本植物病理学会報. 68・1(印刷中). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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