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The Molecular Mechanism of the Cellular Transport of Carotenoids in the silkworm, Bombyx mori

Research Project

Project/Area Number 13460028
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 蚕糸・昆虫利用学
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

TSUCHIDA Kozo  National Institute of Infectious Diseases, Radiological Protection, Senior Researcher, 放射能管理室, 主任研究官 (40231435)

Co-Investigator(Kenkyū-buntansha) FUJII Hiroshi  Kyushu University, Agriculture, Professor, 農学部, 教授 (10038268)
MORIBAYASHI Atsuko  National Institute of Infectious Diseases, Medical Entomology, Senior Researcher, 昆虫医科学部, 主任研究官 (20072944)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥11,600,000 (Direct Cost: ¥11,600,000)
Fiscal Year 2003: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2002: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2001: ¥4,000,000 (Direct Cost: ¥4,000,000)
KeywordsCarotenoid Binding Protein / Lipophorin / Lipid Transport / StAR / Insect / Silkworm / Yellow Hemolymph / Mutants / LTP / カロチノイド / 昆虫 / 脂質輸送
Research Abstract

Fundamental aspects of carotenoid absorption are largely unknown, such as luminal and intracellular factors related to absorb and transport of carotenoid. Carotenoid transport mutants offer unique research tools for studying lipid specific transporter in insects. In the +^Y gene, the transport of the other lipids except carotenoid is normal, only the transfer of lutein is affected. The amount of carotenoids transported to the hemolymph was 100-fold greater in the wild type than in mutants. A carotenoid binding protein (CBP) has been isolated, from Bombyx mori larvae. Immunoblotting analysis and immunocytochemistry confirmed the presence of CBP in larvae with the dominant Y gene only. Immunocytochemistry also verified the localization of CBP in the brush border of the midgut epithelial cells, indicating that CBP might be involved in absorption and transport of carotenoid in the epithelial cell. RFLP mapping analysis shows almost certainly that Y gene encode CBP. In both Y and +^Y mutants, CBP gene and CBP mRNA were found by Southern and Northern hybridization. The difference of sequences of CBP cDNA from Y and +^Y strains was not found. It is not clear why +^Y strain can not make CBP. +^Y might have a mutation on the regulatory region related to CBP expression.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (40 results)

All Other

All Publications (40 results)

  • [Publications] Arrese E.J.: "Lipid storage and mobilization in insects : current status and future directions."Insect Biochemistry and Molecular Biology. 31. 7-17 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Funatsuki K.: "Rapid identification of Bombyx mori cells using PCR amplification following a direct procedure for genomic DNA preparation."Journal of Insect Biotechnology and Sericology. 70. 129-136 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tabunoki H.: "Isolation, characterization and cDNA sequence of a carotenoid binding protein from the silk gland of Bombyx mori."Journal of Biological Chemistry. 277. 32133-32140 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakajima Y.: "Possible horizontal transfer of mariner-like sequences into some invertebrates including lepidopteran insects, a grasshopper and a coral."Journal of Insect Biotechnology and Sericology. 71. 109-121 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] He N.: "Genomic structure and expression analysis of the gene encoding a silkworm basic Kunitz-type chymotrypsin inhibitor."Biochimica et Biophysica Acta. 1628. 71-77 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kotani E.: "Somatic mutation in larvae of the silkworm, Bombyx mori, induced by heavy irradiation to diapause eggs"Journal of Radiation Research. 43. 193-198 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Jouni Z.: "Transfer of cholesterol and diacylglycerol from lipophorin to Bombyx mori ovarioles in vitro : role of the lipid transfer particle."Insect Biochemistry and Molecular Biology. 33. 145-153 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 森林敦子: "ケブカクロバエAldrichina grahami(Aldrich)(Diptera : Calliphoridae)成虫の卵巣発育におけるエクジステロイドホルモン及び脂質の変動について"Medical Entomology and Zoology. 54. 65-71 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakajima, Y.: "Comparison of mariner-like elements among Bombyx mandarina individuals inhabiting East Asia in the light of the segregation of B.mori and B.mandarina genomes."Journal of Wild Silkmoth & Silk. 8. 57-64 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujimoto H.: "Integration of 5' end of the retrotransposon, R2Bm, can be complemented by homologous recombination"Nucleic Acids Research. 32. 1555-1565 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tsuchida K.: "Histochemical characterization of the carotenoid-binding protein of Bombyx mori"Journal of Insect Physiology. 50. 363-372 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tsuchida K.: "The Basis for Colorless Hemolymph and Cocoons in the Y-gene recessive Bombyx. mori Mutants : A Defect in the Cellular Uptake of Carotenoids."Journal of Insect Physiology. 50(In press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Arrese E.J., Canavoso L.E., Jouni Z.E., Pennington J.E., Tsuchida K., Wells M.A.: "Lipid storage and mobilization in insects : current status and future directions."Insect Biochemistry and Molecular Biology. 31. 7-17 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Funatsuki K., K.Hashido, M.Matsunami, Y.Kameoka, K.Iwabuchi, H.Tsukeda, Tsuchida K., N.Takada, Y.Nakajima, H.Maekawa: "Rapid identification of Bombyx mori cells using PCR amplification following a direct procedure for genomic DNA preparation."Journal of Insect Biotechnology and Sericology. 70. 129-136 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tabunoki H., H.Sugiyama, Y.Tanaka, H.Fujii, Y.Banno, Z.Jouni, M.Kobayashi, H.Maekawa, K.Tsuchida: "Isolation, characterization and cDNA sequence of a carotenoid binding protein from the silk gland of Bombyx mori."Journal of Biological Chemistry. 277. 32133-32140 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakajima Y, H.Fujimoto, T.Negishi, K.Hashido, T.Shiino, K.Tsuchida, N.Takada, H.Maekawa: "Possible horizontal transfer of mariner-like sequences into some invertebrates including lepidopteran insects, a grasshopper and a coral."Journal of Insect Biotechnology and Sericology. 71. 109-121 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] He N., M.Yokiyama, H.Fujii, Y.Banno, K.Yamamoto: "Genomic structure and expression analysis of the gene Encoding a silkworm basic Kunitz-type chymotrypsin inhibitor."Biochimica et Biophysica Acta. 1628. 71-77 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kotani E., T.Furusawa, S.Nagaoka, K.Nojima, H.Fujii, Y.Sugimura, M.Ichida, E.Suzuki, A.Nagamatsy, T.Todo, M.Ikenaga: "Somatic mutation in larvae of the silkworm, Bombyx mori, induced by heavy irradiation to diapause eggs."Journal of Radiation Research. 43. 193-198 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Jouni Z., N.Takada, J.Gazard, H.Maekawa, M.A.Wells, K.Tsuchida: "Transfer of cholesterol and diacylglycerol from lipophorin to Bombyx mori ovarioles in vitro : role of the lipid transfer particle."Insect Biochemistry and Molecular Biology. 33. 145-153 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Moribayashi A., Kurahashi H., Sugie H., Katagiri C., Tsuchida K.: "Lipids and ecdysteroids on the ovarian growth of the adult blow fly, Aldrichna grahami"Medical Entomology and Zoology. 54. 65-71 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakajima, Y., T.Nakamura, Y.Banno, H.Fujimoto, K.Hashido, T.Shiino, K.Tsuchida, H.Maekawa: "Comparison of mariner-like elements among Bombyx mandarina individuals inhabiting East Asia in the light of the segregation of B. mori and B. mandarina genomes."Journal of Wild Silkmoth & Silk. 8. 57-64 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujimoto H., Y.Hirukawa, H.Tani, Y.Matsuura, K.Hashido, K.Tsuchida, N.Takada, M.Kobayashi, H.Maekawa: "Integration of 5' end of the retrotransposon, R2Bm, can be complemented by homologous recombination."Nucleic Acids Research. 32. 1555-1565 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tsuchida K., Z.E.Jouni, J.Gardetto, Y.Kobayashi, Tabunoki H., M.Azuma, H.Sugiyama, Takada N., Maekawa H., Banno Y., Fujii H., M.A.Wells: "Histochemical characterization of the carotenoid-binding protein of Bombyx mori."Journal of Insect Physiology. 50. 363-372 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tsuchida K., C.Katagiri, Y.Tanaka, H.Tabunoki, R.Sato, H.Maekawa, N.Takada, Y.Banno, H.Fujii, Z.E.Jouni: "The Basis for Colorless Hemolymph and Cocoons in the Y-gene recessive Bombyx. mori Mutants : A Defect in the Cellular Uptake of Carotenoids."Journal of Insect Physiology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamauchi Y et al.: "cDNA and deduced amino acid sequence of apolipophorin-III from Agrius convolvuli"Applied Entomology and zoology. 36. 21-26 (2001)

    • Related Report
      2003 Annual Research Report
  • [Publications] Funatsuki et al.: "Rapid identification of Bombyx mori cells using PCR amplification following a direct procedure of genomic DNA preparation"Journal of Insect Biotechnology and Sericology. 70. 129-136 (2001)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tabunoki H. et al.: "Isolation, characterization and cDNA sequence of carotenoid binding protein from the silk gland of Bomby mori"Journal of Biological Chemistry. 277. 32133-32140 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakajima N. et al.: "Possible horizontal transfer of mariner like sequences into some invertebrates including lepidopteran insect"Jounal of Insect Biotecnology and Sericology. 71. 109-121 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Jouni Z. et al.: "Transfer of cholesterol and diacylglycerol from lipophorin to Bombyx mori ovarioles in vitro : role of the lipid transfer particles"Insect Biochemistry and Molecular Biology. 33. 145-153 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tsuchida K. et al.: "Characterization of the carotenoid binding protein of the Y gene dominants of Bombyx mori"Journal of Insect Physiology. 50(in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yamauchi Y.: "cDNA and deduced amino acid sequence of apolipophorin-III from Agrius convolvuli"Applied Entomology and Zoology. 35. 21-26 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Funatsuki K.: "Rapid identification of Bombyx mori cells using PCR amplification following a direct for genomic DNA preparation"J. Insect Biotechnology and Sericology. 70. 129-136 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tabunoki H.: "Isolation, Characterization and cDNA Sequence of a Carotenoid Binding Protein from the Silk Gland odf Bombyx mori"J. Biological Chemistry. 277. 32133-32140 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakajima Y.: "Possible horizontal transfer of mariner-like sequences into some invertebrates including lepidopteran insect"J. Insect Biotechnology and Sericology. 71. 109-121 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Jouni Z.: "Transfer of cholesterol and diacylglycerol from lipophorin to Bombyx mori ovarioles in vitro : role of the lipid transfer particle"Insect Biochemistry and Molecular Biology. 33. 145-153 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 森林敦子: "ケブカクロバエ成虫の卵巣発育におけるエクジステロイドホルモン及び孜々湯の変動について"Medical Entomology and Zoology. 54. 1-8 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Arresse E.L.: "Lipid storage and mobilization in insects : current status and future direction"Insect Biochemistry and Molecular Biology. 31. 7-17 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Funatuki K.: "Rapid identification of Bombyx mori cells using PCR amplification following a direct procedure for genomic DNA preparation"Journal of Insect Biotechnology and Sericology. 70.2. 129-136 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Banno Y.: "Inheritance of one cocoon shape mutant named "flossy cocoon" of the silkworm, Bombyx mori"Journal of Insect Biotechnology and Sericology. 70、3. 189-192 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Moribayashi: "Latitudinal variation in the incidence of pupal diapause in Asian and Oceanian populations of the flesh fly, Boettcherisca peregrina"Medical Entomology and Zoology. 52,4. 263-268 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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