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Study on enhancement of wide-range stress tolerance by rhizosphere control on moleculartolerance by rhizosphere control on molecular base.

Research Project

Project/Area Number 13460029
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionHokkaido University

Principal Investigator

OSAKI Mitsuru  Hokkaido Univ., Grad. School of Agr., Prof., 大学院・農学研究科, 教授 (60168903)

Co-Investigator(Kenkyū-buntansha) TAWARAYA Keitarou  Yamagata Univ., Grad. School of Agr., Asso. Prof., 農学部, 助教授 (70179919)
HASHIODOKO Yasuyuki  Hokkaido Univ., Grad. School of Agr., Asso. Prof., 大学院・農学研究科, 助教授 (40281795)
MATSUI Hirokazu  Hokkaido Univ., Grad. School of Agr., Prof., 大学院・農学研究科, 教授 (90109504)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥12,500,000 (Direct Cost: ¥12,500,000)
Fiscal Year 2003: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2002: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2001: ¥4,600,000 (Direct Cost: ¥4,600,000)
Keywordsrhizosphere control / low pH / A1 tolerance / low P tolerance / root exudation / mycorrhiza / haypae / 広域ストレス / 根圏環境制御 / PEPC / 酸性フォスファターゼ / フィターゼ / 形質転換 / プロモーター / 酸性フォスターゼ
Research Abstract

Acid and alkaline soils occupy over half of arable land in the world. Plant growth is inhibited in these soils because of the insolubilization of nutrients and toxicity of inorganic compounds such as A1 and heavy metals. Recently, it is indicated that the plant roots have some strategy to improve the rhizosphere environment (about several μm from root surface). We have studied to increase the capacity of function for rhizosphere regulation. The results of this study are summarized as follows;
1) A cDNA for LASAP2 encoding acid phosphatase secreted from white lupin roots was transformed into tobacco plants. Genomic DNA containing promoter region was transformed into rice plants. These plants were able to secrete the acid phosphatase which had mostly same properties with native mature protein of white lupin roots.
2) To introduce the PEPC (phosphoenolpyruvate carboxylase) gene into rice plants, some gene constructs for transformation using Agrobacterium were designed. For the efficient expression of PEPC, a transgenic rice line containing PEPC gene under regulation of actin promoter was selected. Novel gene for PEPC was isolated from rice plant, and the function was analyzed. The novel PEPC was C3 type and specifically induced in leaves. The identity was about 80% with root type PEPC. The A1 and low P tolerances were improved in PEPC transgenic rice line because overexpression of mRNA and protein for PEPC causes the increase of oxalate secretion from roots.
3) Effect of root exudates of P-deficient onion on hyphal growth of arbuscular mycorrhizal fungi were studied. A1 fraction (Rf:0.47) was isolated by TLC from ethyl acetate fraction of onion root exudates. Spores of Gigaspora margarita were incubated in this fraction and deionized water (control) for 7 days. Length of external hyphae and number of 2nd or higher order branches were higher in A1 fraction than in control.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (39 results)

All Other

All Publications (39 results)

  • [Publications] Watanabe, T: "Al uptake kinetics in roots of Melastoma malbathricum L. - an Al accumulator plant"Plant and Soil. 231(2). 283-291 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takuro Shinanoi: "Characteristics of phosphoenolpyruvate phosphatase purified from Allium cepa"Plant Science. 161. 861-869 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Watanabe, T: "Influence of aluminum and phosphorus on growth and xylem sap composition in Malastoma malbathricum"Plant and Soil. 237. 63-70 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Watanabe, T: "Mechanisms of adaptation to high Al condition in native plant species growing in acid soils : A review"Communications in Soil Science and Plant Analysis. 33(7&8). 1247-1260 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Watanabe, T: "Role of organic acids in aluminum accumulation and plant growth in Melastoma malbathricum L."Tree Physiology. 22. 785-792 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kai, M: "Molecular cloning and expression of phosphate transporter gene in Nicotiana tabacum L."Plant Science. 163(4). 837-846 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hashidoko, Y: "(Note) Soft gel medium solified with gellan gum for preliminary screening for root-associating, free-living nitrogen-fixing bacteria inhabiting the rhizoplaine of plants"Biosci.Biotechnol.Biochem. 66(10). 2259-2263 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Wasaki, J: "Secreted acid phosphatase is expressed in cluster roots of lupin in response to phosphorus deficiency"Plant and Soil. 248. 129-136 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Jun Wasaki: "Expression of the OsPI1 gene, cloned from rice roots using cDNA microarray, rapidly responds to phosphorus status"New Phytologist. 158. 239-248 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Jun Wasaki: "Transcriptomic analysis of metabolic changes by phosphorus stress in rice plant roots"Plant, Cell and Environment. 26. 1515-1523 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tawaraya, K: "Arbuscular mycorrhizal colonization of tree species grown in peat swamp forests of Central Kalimantan, Indonesia"Forest Ecology and Management. 182. 381-386 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takuya Yamamura: "Possibility of rhizosphere regulation using acid phosphatase and organic acid for recycling phosphorus in sewage sludge"Soil Science and Plant Nutrition. 50(1). 77-83 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Masahito Nanamori: "Low Phosphorus Tolerance Mechanisms : Phosphorus Recycling and Photosynthate Partitioning in the Tropical Forage Grass, Brachiaria Hybrid cultivar Mulato Compared with Rice"Plant and Cell Physiology. 45(4). 460-469 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hans Lambers: "Structure and Functioning of cluster Roots and Plant Responses to phosphate Deficiency"Kluwer Academic Publishers. 333 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Watanabe, T.: "A1 uptake kinetics in roots of Malastoma malbathricum L.-an A1 accumulator plant"Plant and Soil. 231(2). 283-291 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takuro Shinanoi: "Characteristics of phosphoenolpyruvate phosphatase purified from Allium cepa"Plant Science. 161. 861-869 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Watanabe, T.: "Influence of aluminum and phosphorus on growth and xylem sap composition in Malastoma malbathricum"Plant and Soil. 237. 63-70 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Watanabe, T.: "Mechanisms of adaptation to high A1 condition Communications in Soil Science and in native plant species growing in acid soils : A review"Plant Analysis. 33(7&8). 1247-1260 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Watanabe, T.: "Role of organic acids in aluminum accumulation and plant growth in Melastoma malbathricum L"Tree Physiology. 22. 785-792 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kai, M.: "Molecular cloning and expression of phosphate transporter gene in Nicotiana tabacum L."Plant Science. 168(4). 837-846 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hashidoko, Y: "(Note) Soft gel medium solified with gellan gum for preliminary screening for root-associating, free-living nitrogen fixing bacteria inhabiting the rhizoplaine of plants"Biosci.Biotechnol.Biochem.. 66(10). 2259-2263 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Wasaki, J.: "Secreted acid phosphatase is expressed in cluster roots of lupin in response to phosphorus deficiency"Plant and Soil. 248. 129-136 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Jun Wasaki: "Expression of the OsPI1 gene, cloned from rice roots using cDNA microarray, rapidly responds to phosphorus"New Phytologist. 158. 239-248 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Jun Wasaki: "Transcriptomic analysis of metabolic changes by phosphorus stress in rice plant roots"Plant, Cell and Environment. 26. 1515-1523 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tawaraya, K.: "Arbuscular mycorrhizal colonization of tree species grown in peat swamp forests of Central Kalimantan, Indonesia"Forest Ecology and Management. 182. 381-386 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takuya Yamamura: "Possibility of rhizosphere regulation using acid phosphatase and organic acid for recycling phosphorus in sewage sludge"Soil Science and Plant Nutrition. 50(1). 77-83 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Masahito Nanamori: "Low Phosphorus Tolerance Mechanisms : Phosphorus Mechanisms : Phosphorus Recycling and Photosynthate Partitioning in the Tropical Forage Grass, Brachiaria Hybrid cultivar Mulato Compared with Rice"Plant and Cell Physiology. 45(4). 460-469 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Wasaki, J., et al.: "Transcriptomic analysis of metabolic changes by phosphorus stress in rice plant roots."Plant, Cell and Environment. 26. 1515-1523 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yamamura, T., et al.: "Possibility of rhizosphere regulation using acid phosphatase and organic acid for recycling phosphorus in sewage sludge."Soil Science and Plant Nutrition. 50. 77-83 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tawaraya, K. et al.: "Arbuscular mycorrhizal colonization of tree species grown in peat swamp forests of Central Kalimantan, Indonesia."Forest Ecology and Management. 182. 381-386 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Begum, H.H., et al.: "Role of phosphoenolpyruvate carboxylase in the adaptation of a tropical forage grass, Brachiaria hybrid, to low phosphorus acid soils."Journal of Plant Nutrition. (in press).

    • Related Report
      2003 Annual Research Report
  • [Publications] Begum, H.H., et al.: "Aluminum tolerant mechanisms in phosphoenolpyruvate carboxylase (PEPC) transgenic rice."Plant and Soil. (in press).

    • Related Report
      2003 Annual Research Report
  • [Publications] Kai, M., et al.: "Molecular cloning and expression of phosphate transporter gene in Nicotiana tabacum L"Plant Science. 163(4). 837-846 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Wasaki, J., et al.: "Secreted acid phosphatase is expressed in cluster roots of lupin in response to phosphorus deficiency"Plant & Soil. 248. 129-136 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tawaraya, K. et al.: "Arbuscular mycorrhizal colonization of tree species grown in peat swamp forests of Central Kalimantan, Indonesia"Forest Ecology and Management. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Toshihiro Watanabe: "Influence of aluminum and phosphorus on growth and xylem sap composition in Melastoma malabathricum L"Plant and Soil. 237・1. 63-70 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takuro Shinano: "Characteristics of phosphoenolpyruvate phosphatase purified from Allium cepa"Plant Science. 161・5. 861-869 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Susumu Hiraga: "A large family of Class III plant peroxidases"Plant Cell Physiology. 42・5. 462-468 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Jun Wasaki: "Secreted acid phosphatase is expressed in cluster roots of lupin in response to phosphorus deficiency"Plant and Soil. in press. (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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