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Biochemistry and physiology of the inorganic nutrients working in the apoplast of higher plants

Research Project

Project/Area Number 13460031
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

MATOH Toru  Kyoto University, Graduate School of Agriculture, Associate Professor, 農学研究科, 助教授 (50157393)

Co-Investigator(Kenkyū-buntansha) MA Jean-feq  Kagawa University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (80260389)
FUJIWARA Toru  University of Tokyo, Graduate School of Agriculture and Life Science, Associate Professor, 農学生命科学研究科, 助教授 (80242163)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥9,500,000 (Direct Cost: ¥9,500,000)
Fiscal Year 2002: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 2001: ¥5,000,000 (Direct Cost: ¥5,000,000)
KeywordsBovon / Calcium / Auminium / Apoplast / Cell wall / Edaphic stress / 土壌ストレス / ペクチン / KDO合成酵素 / ラムノガラクツロナンII / マイクロアレイ
Research Abstract

We have revealed that the essential plant nutrient element boron (B) works to cross-link pectic polysaccharide chains at the rhamnogalacturonan II (RG-II) sites along the chain. In the cross-link, Ca^<2+> ions are also involved to connect the chains, but Al ion interferes with the bonding. Matoh and his colleague purified some enzymes which are responsible for the sugars which particular to RG-II, a KDO synthase, and its DNA was cloned and sequenced. Fujiwara et al revealed that B is actively taken up by higher plants through a B-specific membrane proteins. The protein and its DNA was cloned and sequenced. The B transporter works in the endodermis. Ma studied the binding sites for Al in barley root cell walls. Aluminum ion binds to cell wall pectic polysaccharides, but it works the adverse effect only by which binds to the outer epidermis.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Matoh, T.: "Boron function in plant cell walls"Boron in Plant and Animal Nutrition. 143-155 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matoh, T.: "Boron in Plant Nutrition and Cell Wall Development"Plant Nutrition Aquisition New Perspective. 227-250 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Qin Yu, Hlavacka: "Short-term boron deprivation inhibits"Plant Physiol.. 130. 415-421 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takano, J.: "Arabidopsis boron transporter for xylem loading"Nature. 420. 337-340 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matoh T and Kobayashi M,: "Boron function in plant cell walls-Research progress since 1997"Boron in Plant and Animal Nutrition (Eds. , Goldbach HE, Rerkasem B, Wimmer MA, Brown PH, Thellier M & Bell RW, Kluwer Academic/Plenum Publishers, New York 2002). 143-155

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matoh T: "Boron in Plant Nutrition and Cell Wall Development"Plant Nutrition Acquisition New Perspective (Eds, Ae N, Arihara J, Okada K & Srinivasan A, Springer-Verlag Tokyo (2001)). 227-250

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Qin Yu, Hlavacka A, Matoh T, Volkmann D, Menzel D, Goldbach HE, Baluska F.: "Short-term boron deprivation inhibits endocytosis of cell wall pectins in meristematic cells of maize and wheat root apices"Plant Physiol. 130. 415-421 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takano, J., Noguchi, K., Yasumori, M., Kobayashi, M., Gajdos, Z., Miwa, K, Hayashi, H., Yoneyama, T. and Fujiwara T.: "Arabidopsis boron transporter for xylem loading"Nature. 420. 387-840 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Masaru Kobayashi, et al.: "Purification and cDNA Cloning of UDP-D-Glucuronate Carboxylyase (UDP-D-xylose Synthase) from Pea Seedlings"Plant Cell Physiology. 43. 1259-1265 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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