| Project/Area Number |
13460079
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Hokkaido University |
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Principal Investigator |
ARAI Katsutoshi Hokkaido Univ., Graduate School of Fisheries Sciences, Prof., 大学院・水産科学研究科, 教授 (00137902)
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| Co-Investigator(Kenkyū-buntansha) |
OKUMURA Sei-ichi Kitasato Univ., School of Fisheries Science, Lec., 水産学部, 講師 (60224169)
YAMAHA Etsuro Hokkaido Univ., Field Science Center of Northern Biospere, Prof., 北方生物圏フィールド科学センター, 教授 (60191376)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥12,700,000 (Direct Cost: ¥12,700,000)
Fiscal Year 2003: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2002: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2001: ¥8,500,000 (Direct Cost: ¥8,500,000)
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| Keywords | Mosaic / Polyploid / Chromosome manipulation / Breeding / Flow cytometry / Micromanipulation of embryo / Chimera / Fish and Shellfish |
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| Research Abstract |
1) In masu Salmon embryos, subjected to the treatment with hydrostatic pressure to inhibit the first cleavage, delayed and aberrant cell divisions probably due to the formation of multipolar sindle, fragmentation of chromosomes and other factors were observed. The occurrence of inviable mosaic embryos was caused by these abnormalities.
2) Masu salmon developed from the treatment for tetraploidization by inhibition of the first cleavage frequently exhibited diploid-tetraploid mosaicisms in various organs and tissues. When diploid-tetraploid salmon had gonads including germ cells originated from tetraploid blastomeres, functional diploid gametes were generated. Mass production of triploid progeny was successfully realized by the cross between normal and mosaic parents.
3) Although haploid embryos were inviable in fish, infrequent spontaneous haploid-diploid mosaics often exhibit the recovery of survival potential. The recovery of survival capacity by adding diploid cells to haploid embryos was experimentally confirmed by the production of viable haploid-diploid goldfish chimeras, which were induced by transplantation of blastodisc.
4) In wild populations of crucian carp and loach, a few mosaic individuals were found out. Diploid-triploid mosaic loaches were disclosed to generate fertile unreduced diploid sperm, which were genetically identical, i.e. clonal. The occurrence of haploid-diploid, diploid-tetraploid and other mosaics was also observed in aquaculture farm and egg quality was considered as a cause of mosaicism.
5) Triploid abalone were induced by inhibition of the second polar body release after fertilization and their ploidy status was studied for two years. Diploid-triploid mosaicism was exclusively detected in epipodial tentacle tissues and ploidy Status was changed from triploid to diploid or vice versa. However, no such alteration was not seen in other tissues.
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