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GnRH in fish leukocytes

Research Project

Project/Area Number 13460084
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionThe University of Tokyo

Principal Investigator

SUZUKI Yuzuru  The University of Tokyo, Graduate School of Agricultural and Life Science, Professor, 大学院・農学生命科学研究科, 教授 (40107412)

Co-Investigator(Kenkyū-buntansha) SUETAKE Hiroaki  The University of Tokyo, Graduate School of Agricultural and Life Science, Research associate, 大学院・農学生命科学研究科, 助手 (00334326)
AIDA Katsumi  The University of Tokyo, Graduate School of Agricultural and Life Science, Professor, 大学院・農学生命科学研究科, 教授 (50012034)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥14,800,000 (Direct Cost: ¥14,800,000)
Fiscal Year 2003: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2002: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2001: ¥8,400,000 (Direct Cost: ¥8,400,000)
KeywordsGnRH / leukocyte / fish / reproductive endocrine system / immunity / fugu / コイ / 生殖 / 性成熟 / 生体防御
Research Abstract

Fugu, which has very small genome size, was found to have three types of GnRH, i.e., sbGnRH, sGnRH and cGnRH II, and three GnRH receptors (GnRH-R). We determined their primary structures by cDNA cloning. RT PCR analyses showed that sbGnRH and sGnRH are expressed in various tissues including lymphoid organs and leukocytes, while cGnRH II is expressed only in the kidney, the head kidney and the spleen in addition to the brain, indicatin. Expression of GnRH-R was also recognized in variety of tissues including immune organs and leukocytes. Significance of GnRH in immune system has thus been clearly recognized. Each Affinity of the three types of GnRH against the three GnRH-R expressed on COS cells was tested, and found that all three receptors showed higher affinities against sGnRH and cGnRH II, than against sbGnRH. These results indicate a large variety of the GnRH functions via GnRH-R.
It is essential to classify the lymphocytes for the analysis on the function of GnRH in the immune system. We determined several immune factors of lymphocytes such as, CD3, CD4, CD8, IL-8, IL-12, TCRγ, IgM, and IgD, and now analyzing the effects of immunomodulators, such as PHA, ConA, Poly I : C, LPS and PMA, against those immune factors of lymphocytes, including GnRH.
In addition to these observations, analyses on the relationships between reproductive endocrine system and leukocyte functions. Downregulation of the expression of tyrosine phosphatases by GnRH was also discussed

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (46 results)

All Other

All Publications (46 results)

  • [Publications] Okubo, Aida 他: "Identification and characterization of two distinct GnRH receptor subtypes in teleost, the medaka, Oryzias latipes."Endocrinology. 142. 4729-4739 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kim, Aida, 他: "Effects of olfactory tract section on the immunohistochemical distribution of brain GnRH fibers in the female goldfish, Carassius auratus"Zool.Sci.. 18. 241-248 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okubo, Aida: "Gonadotropin-releasing hormones (GnRHs) on a primitive teleost, the arowana : phylogenetic evidence that three, paralogous lineages of GnRH occurred prior to the emergence of teleosts."Gen.Comp.Endocrinol.. 124. 125-133 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okubo, Aida 他: "Structural characterization of GnRH loci in the medaka genome."Gene. 293. 181-189 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Amano, Aida 他: "Molecular cloning of three cDNAs encoding different GnRHs in the brain of barfin flounder."Gen.Comp.Endoclinol.. 126. 325-333 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okubo, Aida: "Gonadotropin-releasing hormone gene products downregulate the expression of their neighboring genes that encode protein tyrosine phosphatases α and ε."Biochem.Biophys.Res.Communications. 312. 531-536 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Saha, Usami, Suzuki: "A double staining flow cytometric assay for the detection of steroid induced apoptotic leucocytes in common carp (Cyprinus carpio)."Dev.Comp.Immunol.. 27. 351-363 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshiura, Suetake, Suzuki 他: "Identification and characterization of Fugu orthologues of mammalian interleukin-12 subunits."Immunogenetics. 55. 296-306 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Saha, Suetake, Suzuki: "Characterization and expression of the immunoglobulin light chain in the Fugu : evidence of a solitaire type."Immunogenetics. 56. 47-55 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Saha, Usami, Suzuki: "In vitro effects of steroid hormones on the IgM-secreting cells and IgM-secretion in common carp (Cyprinus carpio)."Fish Shellfish Immunol.. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suetake, Suzuki, Aida 他: "Differntial expression of two gonadotropin (GTH) β subunit genes during ovarian maturation induced by repeated injection of salmon GTH in the Japanese eel"Fisheries Sci. 68. 290-298 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okubo, Suetake, Aida: "Three mRNA species for mammarian-type gonadotropin-releasing hormone in the brain of the eel, Anguilla japonica"Mol.Cell.Endocrinol.. 192. 17-25 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Saha, Suetake, Suzuki 他: "Relationships between immune and reproductive endocrine systems in fish."Fisheries Sci.. 68 suppleII. 1135-1138 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Saha, Usami, Suzuki: "Seasonal changes in the immune activities of common carp (Cyprinus carpio)."Fish Physiol.Biochem.. 26. 379-387 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okubo, Aida 他: "Conserved physical linkage of GnRH-R and RBM8 in the medaka and human genomes."Biochem.Biophys.Res.Communications. 293. 327-331 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 鈴木譲, 末武弘章他: "海洋生物の生体防御"月刊海洋. 号外29. 70-75 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okubo, Aida 他: "A novel third gonadotropin-releasing normore receptor in the medaka Oryzias latipes : evolutionary and fuctional implications."Gene. 314. 121-131 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Amano, Aida 他: "Ontogenic origin of Salmon GnRH neurons in the ventral telencephalon and the preoptic area in masu salmon."Gen.Comp.Endoclinol. 127. 256-262 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okubo, K., Nagata, S., Ko, R., Kataoka, H., Yoshiura, Y, Mitani, H., Kondo, M., Naruse, K., Shima, A., Aida, K.: "Identification and characterization of two distinct GnRH receptor subtypes in teleost, the medaka, Oryzias latipes."Endocrinology. 142. 4729-4739 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kim, M.H., Kobayashi, M., Oka, Y, Amano, M., Kawashima, S., Aida, K.: "Effects of olfactory tract section on the immunohistochemical distribution of brain GnRH fibers in the female goldfish, Carassius auratus."Zool.Sci.. 18. 241-248 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okubo, K., Aida, K.: "Gonadotropin-releasing hormones (GnRHs) on a primitive teleost, the arowana : phylogenetic evidence that three, paralogous lineages of GnRH occurred prior to the emergence of teleosts"Gen.Comp.Endocrinol.. 124. 125-133 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okubo, K., Mitani, H., Naruse, K., Kondo, M., Shima, A., Tanaka, M., Asakawa, S., Shimizu, N., Yoshiura, Y., Aida, K.: "Structural characterization of GnRH loci in the medaka genome"Gene. 293. 181-189 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Amano, M., Takahasi, A., Yamanome, T., Okubo, K., Aida, K., Yamamori, K.: "Molecular cloning of three cDNAs encoding different GnRHs in the brain of barfin flounder"Gen.Com.Endoclinol.. 126. 325-333 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Amano, M., Okubo, K., Ikuta, K., Kitamura, S., Okuzawa, K., Yamada, H., Aida, K., Yamamori, K.: "Ontogenic origin of salmon GnRH neurons in the ventral telencephalon and the preoptic area in masu salmon."Gen.Comp.Endoclinol.. 127. 256-262 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suetake, H., Okubo, K., Sato, N., Yoshiura, Y, Suzuki, Y, Aida, I i: "Differential expression of two gonadotropin (GTH) β subunit genes during ovarian maturation induced by repeated injection of salmon GTH in the Japanese eel, Anguilla japonica."Fisheries Sci.. 68. 290-298 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okubo, K., Suetake, H., Aida, K.: "Three mRNA species for mammalian-type gonadotropin-releasing hormone in the brain of the eel, Anguilla japonica"Mol.Cell.Endocrinol.. 192. 17-25 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Saha, N.R., Usami, T., Saito, T., Suetake, H., Suzuki, Y.: "Relationships between immune and reproductive endocrine systems in fish."Fisheries Sci.. 68 suppl.II.. 1135-1138 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Saha, N.R., Usami, T.and Suzuki, Y.: "Seasonal changes in the immune activities of common carp (Cyprinus carpio)"Fish Physiol.Biochem.. 26. 379-387 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okubo, K., Mitani, H., Naruse, K., Kondo, M., Shima, A., Tanaka, M., Aida, K.: "Conserved physical linkage of GnRH-R and RBM8 in medeka and human genomes"Biochem.Biophys.Res.Communications. 293. 327-331 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suzuki, Y, Suetake, H., Tasumi, S., Tsutsui, S., Saha, H.R., Usami, T., Saito, T., Hamuro, K., Yumoto, S., Nakamura, O., Watanabe, T.: "Kaiyoseibutu no seitaibougyo"Gekkan Kaiyo. Suppl.29. 70-75 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okubo, K., Ishii, S., Ishida, J., Mitani, H., Naruse, K., Kondo, M., Shima, A., Tanaka, M., Asakawa, S., Shimizu, N., Aida, K.: "A novel third gonadotropin-releasing hormone receptor in the medaka Oryzias latipes : evolutionary and fuctional implications"Gene. 314. 121-131 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okubo, K, Aida, K.: "Gonadotropin-releasing hormone gene products downregulate the expression of their neighboring genes that encode protein tyrosine phosphatases a and β"Biochem.Biophys.Res.Communications.. 312. 531-536 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Saha, N.R., Usami, T., Suzuki, Y.: "A double staining flow cytometric assay for the detection of steroid induced apoptotic leucocytes in common carp (Cyprinus carpio)"Dev.Comp.Immunol.. 27. 351-363 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshiura, Y., Kiryu, I., Fujiwara, A., Suetake, H., Suzuki, Y., Nakanishi, T., Ototake, M: "Identification and characterization of Fugu orthologues of mammalian interleukin-12 subunit"Immunogenetics. 55. 296-306 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Saha, N.R., Suetake, H., Suzuki, Y.: "Characterization and expression of the immunoglobulin light chain in the Fugu : evidence of a solitaire type"Immunogenetics. 56. 47-55 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Saha, N.R., Usami, T, Suzuki, Y.: "In vitro effects of steroid hormones on the IgM-secreting cells and IgM-secretion in common carp (Cyprinus carpio)"Fish Shellfish Immunol.. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Saha, Usami, Suzuki: "A double staining flow cytometric assay for the detection of steroid induced apoptotic leucocytes in common carp"Dev.Comp.Immunol.. 27. 351-363 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yoshiura, Fujiwara, Suzuki, Nakanishi 他: "Identification and characterization of Fugu orthologues of mammalian interleukin-12 subunits."Immunogenetics. 55. 296-306 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Saha, Usami, Suzuki: "In vitro effects of steroid hormones on the IgM-secreting cells and IgM-secretion (Cyprinus carpio)"Fish Shellfish Immunol.. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Okubo, Suetake, Aida 他: "The GnRH system in teleosts"Aquatic Genomics-Step Toward a Greagt Future. 244-262 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Okubo, Aida: "Gonadotropin-releasing hormone gene products downregulate the expression of genes that encode protein tyrosine phosphatases α and β"Biochem.Biophysical Res.Communications. 312. 531-536 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Saha, Usami, Saito, Suetake, Suzuki: "Relationships between immune and reproductive endocrine systems in fish"Fisheries Science. 68,supple II. 1134-1138 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Suetake, Okubo, Sato, Yoshiura, Suzuki, Aida: "Differential expression of two GTH β subunit genes during ovarian maturation induced by repeated injection of salmon GTH in the Japanese eel"Fisheries Science. 68. 290-298 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Saha, Usami, Suzuki: "A double staining flow cytometric essay for the detection of steroid induced apoptotic leucocytes in common carp"Developmental and Comparative Immunology. (印刷中).

    • Related Report
      2002 Annual Research Report
  • [Publications] 鈴木譲, 末武, 田角, 筒井, サハ 他: "海洋生物の生体防御"月刊海洋. 号外29. 70-75 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakamura, et al.: "Decrease of transport of orally administered protein into blood circulation in dev Anguilla Japonica"Fisheries Science. 67. 863-869 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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