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Studies on the Water Purification in the Coastal Areas of Inner Bay Using Nitrifying and Denitrifying Bacteria in the Tidal Bottom-Mud

Research Project

Project/Area Number 13460107
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Irrigation, drainage and rural engineering/Rural planning
Research InstitutionSaga University

Principal Investigator

SEGUCHI Masahiro  Saga University, Faculty of Agriculture, Professor, 農学部, 教授 (20093974)

Co-Investigator(Kenkyū-buntansha) KANDA Kohzo  Saga University, Faculty of Agriculture, Professor, 農学部, 教授 (00117095)
KATO Fumio  Saga University, Faculty of Agriculture, Professor, 農学部, 教授 (90109223)
KATO Osamu  Saga University, Faculty of Agriculture, Professor, 農学部, 教授 (40038295)
TABATA Masaaki  Saga University, Faculty of Science and Engineering, Professor, 理工学部, 教授 (40039285)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥11,600,000 (Direct Cost: ¥11,600,000)
Fiscal Year 2003: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2002: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2001: ¥5,500,000 (Direct Cost: ¥5,500,000)
KeywordsAriake Sea / tidal flat / water purification / nitrifying bacteria / denitrifying bacteria / inorganic nitrogen / Mechaelis-Menten equation / 底泥 / 硝化・脱窒 / N除去率 / バクテリア / MPN法 / 硝化 / 脱窒 / アセチレンブロック法 / 培養 / アンモニア態窒素 / 硝酸態窒素 / 硝化速度 / ミカエリスーメンテン式
Research Abstract

The purposes of the present research are to clarify the relationships between the biological activity of nitrifying and denitrifying bacteria and various environmental factors in the tidal bottom-mud, and to use the nitrification and denitrification processes maintained by the metabolism of these bacteria in order to purify water of the coastal areas in the inner bay. The conclusions obtained by the present research during three years from 2001 to 2002 are summari zed as follows :
(1)A kind of nitrifying bacteria and two kinds of denitrifying bacteria with high biological activity were selected and isolated from the tidal bottom -mud i n the Ariake Sea. Moreover, the properties of these bacteria were clarified.
(2)The relationships between the nitrification and denitrification rates in the tidal bottom -mud and control factors such as substrate concentr ation, temperature, pH, reduction potential were clarified. A Mechaelis -Menten equation was valid for the relation between nitrification and denitrification rates, substrate concentration and temperature. This equation indicated the optimum temperature for nitrification and denitrification rates and these Timit values.
(3)A method for removal of inorganic nitrogen compounds i n the waste water using the tidal bottom-mud. where many nitrifying and denitrifing bacteria with high biological activity lived was proposed. Moreover, a trial device for its removal based on this method was made.
(4)The relationships between N-removal rates of this trial device and NH_4^+ concentration, temperature, flow, mud-content, pH and number of nitrifying and denitrifying bacteria were commanded experimentally.
(5)The utility of the removal device was verified and the extent power of water purification of the tidal bottom -mud in the Ariake Sea was confirmed. On the other hand, the issues for the practical use of the removal device were also found.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Elivis Nyarko: "Interactions of tetracationic mercury(II), cadmium(II) and lead(II) porphyrins with DNA and their effects on DNA cleavage"Journal of Porphyrins and Phthalocyanines. 5. 873-880 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 瀬口 昌洋: "泥質干潟におけるマクロベントスの巣穴形成とその硝化・脱窒過程への影響"水工学論文集. 46. 1169-1171 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Masahiro Seguchi: "Effects of Temperature and Nitrate Concentration on Denitrification Capacity of Coastal Mud in the Ariake Sea"Bulletin of the Faculty of Agriculture, Saga University. 87. 79-86 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hamid-Reza Kariminiaae-Hamedaani: "Wastewater Treatment with Bacteria Immobilized onto a Ceramic in an Aerated System"Journal of Bioscience and Bioengineering. 95(2). 128-132 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Masaaki Tabata: "Enhanced conformational changes in DNA in the presence of mercury(II),cadmium(II),and lead(II) porphyrins"Journal of Inorganic Biochemistry. 94. 50-58 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hamid-Reza, Kariminiaae-Hamedaani: "Denitrification Activity of the Bacterium Pseudomonas sp.ASM-2-3 Isolated from the Ariake Sea Tideland"Journal of Bioscience and Bioengineering. 97(1). 39-44 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 瀬口 昌洋: "干潟域底泥による水中の無機態窒素化合物の除去特性"水工学論文集. 48. 1297-1302 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Seguch Masahiro: "Water Purification and Removal Characteristics of Inorganic Nitrogen in a Tidal Mud Flat"Bulletin of the Faculty of Agriculture, Saga University. 89. 157-168 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Elivis Nyarko: "Interactions of tetracationic mercury (II), cadmium( II) and lead(II) porphyrins with DNA and their effects on DNA cleavage"Journal of Porphyrins and Phthal-ocyanines. 5. 873-880 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Masahiro Seguchi: "Burrow -Formation by Macrobenthos in the Muddy. Tidal Flat and its Effects on the Nitrification-Denitrification Process"Annual Journal of Hydraulic Engineering. 46. 1169-1174 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Masahiro Seguchi: "Effects of Temperature ands Nitrate Concentration on enitrification Capacity of Coastal Mud in the Ariake Sea"Bulletin of the Faculty of Agriculture, Saga University. 87. 79-86 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hamid-Reza Kariminiaae-Hamedaani: "Waste water Treatment with Bacteria Immobilized onto a Ceramic in an Aerated System"Journal of Bioscience and Bio-engineering. 95(2). 128-132 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Masaaki Tabata: "Enhanced conformational changes in DNA in the presence of mercury (II), cadmiun (II), and lead (II) porphyrins"Journal of Inorganic Biochemistry. 94. 50-58 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hamid-Reza Kariminiaae-Hamedaani: "Denitrification Activity of the Bacterium Pseudomonas sp. ASM-2-3 Isolated from the Ariake Sea Tideland"Journal of Bioscience and Bio-engineering. 97(1). 39-44 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Masahiro Seguchi: "Removal Properties of Inorganic Nitrogen Compounds in the Water Using a Tidal Bottom -Mud"Annual Journal of Hydraulic Engineering. 48. 1297-1302 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Masahiro Seguchi: "Water Purification Function and Removal Characteristics of Inorganic Nitrogen in a Tidal Mud Flat"Bulletin of the Faculty of Agriculture, Saga University. 89. 157-168 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 瀬口昌洋: "干潟域底泥による水中の無機態窒素化合物の除去特性"水工学論文集. 48. 1297-1302 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Seguchi M.: "Water Purification and Removal Characteristics of Inorganic Nitrogen in a Tidal Mud Flat"Bull.Fac.Agr.Saga Univ.. 89. 157-168 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] H-R.Kariminiaae-Hamedaani: "Denitrification Activity of the Bacterium Pseudomonas sp.ASM-2-3 Isolated from the Ariake Sea Tideland"J.Biosci.Bioeng.. 97(1). 39-44 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Seguchi, M.Koriyama, O.Deguchi: "Effects of Temperature and Nitrate Concentration on Denitrification Capacity of Coastal Mud in the Ariake Sea"Bulletin of the Faculty of Agriculture, Saga University. 87. 79-86 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] N.H.Chung, J.Nishimoto, O.Kato, M.Tabata: "Selective extraction of thallium(III) in the presence of gallium(III), indium(III) and antimony(III) by salting out of a aqueous mixture of 2-propanol"Analytica Chimica Acta. 477. 243-249 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 瀬口昌洋, 郡山益美, 加藤 治: "泥質干潟におけるマクロベントすの巣穴形成とその硝化・脱窒過程への影響"水工学論文集. 46. 1169-1174 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y.WG.WU, M.Tabata, T.Takamuku: "An Extended Johnson-Furter Equation to Salting-out Phase Separation of Aqueous Solution of Water-Miscible Organic Solvents"Fluid Phase Equil. 192. 1-12 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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