| Project/Area Number |
13460131
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TAKEUCHI Yukari The University of Tokyo, Graduate School of Agricultural and Life Sciences, Associate Professor, 大学院・農学生命科学研究科, 助教授 (10240730)
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| Co-Investigator(Kenkyū-buntansha) |
MORI Yuji The University of Tokyo, Graduate School of Agricultural and Life Sciences, Professor, 大学院・農学生命科学研究科, 教授 (40157871)
KIKUSUI Takehumi The University of Tokyo, Graduate School of Agricultural and Life Sciences, Assistant, 大学院・農学生命科学研究科, 助手 (90302596)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥12,500,000 (Direct Cost: ¥12,500,000)
Fiscal Year 2003: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2002: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2001: ¥6,700,000 (Direct Cost: ¥6,700,000)
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| Keywords | Dog / Temperament / Neurotransmitter / Temperament-related gene / Genetic Polymorphism / Single Nucleotide Polymorphism / Guide Dog / Behavioral Trait |
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| Research Abstract |
This study was conducted to reveal the genetic background of canine temperament. We first collected blood samples and behavioral scores of candidate guide dogs at the "Guide Dogs for the Blind" institution in USA. The trainers regarded aggression more important than anxiety when they judged the suitability as the guide dog. We then sequenced some target genes (neurotransmitter-associated genes) to search for genetic polymorphisms of these genes. We found several missence SNPs at the coding regions of identified genes, such as monoamine oxidase subtypes A (MAOA) and B (MAOB), serotonin transporter, 5-hydroxytriptamine receptor (5-HTR) 1B, 2A, and 2C. To analyze the breed differences of these polymorphisms, we collected blood samples and carried out a questionnaire survey to owners via veterinary practitioners. There were breed differences in all the identified polymorphisms among 7 representative breeds. By the combining the questionnaire survey and the genetic analysis, we found the relationships between the polymorphism of dopamine D4 receptor gene and "over-attachment to the owner in an unfamiliar place", that of 5HTR1B gene and "aggression". These findings may suggest that polymorphisms of neurotransmitter-associated genes are related to some of the behavioral traits in dogs, which may be responsible for inter-breeds as well as intra-breed differences of behavior predisposition.
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