| Project/Area Number |
13460132
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Shinshu University |
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Principal Investigator |
ONO Tamao Shinshu University, Faculty of Agriculture, Professor, 農学部, 教授 (10177264)
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| Co-Investigator(Kenkyū-buntansha) |
KAGAMI Hiroshi Shinshu University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (80308303)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥16,900,000)
Fiscal Year 2003: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2002: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2001: ¥9,200,000 (Direct Cost: ¥9,200,000)
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| Keywords | chicken / quail / Blue-breasted quail / embryogenesis / primordial germ cells / chimera / PCR / in situ hybridization |
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| Research Abstract |
In order to rescue the endangered species, and production and preservation of variable breeds in bird, interspecific transfers of primoridal germ cells (PGCs) have been performed. We developed the protocol to obtain a large amount of PGCs from gonads of chicken and quail embryos using PGC specific monoclonal antibodies (anti-SSEA1 and QCR1) and magnetism activated cell sorting (MACS). MACS treatment greatly increased the population ratio of avian PGCs in gonadal cells, resulting improved gonadal migration in recipient embryos. We have worked toward practical use of PCR and in situ hybridization to identify the species specific cell (genome) type among chickens, quail and blue-breasted quail. PGCs of chick embryos were transferred into quail embryos, and the chicken-genome-specific PCR product was observed in the semen of chick-PGC-transferred chimeric quail. We made a progeny test. Females of the putative chimeras were artificially inseminated with chicken semen. We obtained hatchlings but PCR analysis showed that they were hybrid between chicken sperm and donor derived quail ovum. Silky PGCs were introduced to White Leghorn chickens and the prpgeny test is underway.
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