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Regulation for growth and differentiation of somatic cells by signals derived from oocytes

Research Project

Project/Area Number 13460133
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionKyushu University

Principal Investigator

HATTORI Masa-aki  Kyushu University, Faculty of Agriculture, Prof, 農学研究院, 教授 (60175536)

Co-Investigator(Kenkyū-buntansha) KATO Yukio  Meiji University, Faculty of Agriculture, Prof, 農学部, 教授 (30114177)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥13,300,000 (Direct Cost: ¥13,300,000)
Fiscal Year 2003: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2002: ¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 2001: ¥4,900,000 (Direct Cost: ¥4,900,000)
KeywordsNitric Oxide / Oocyte / Gene Expression / Cell Proliferation and Differentiation / Ovary / Hormone / Oocyte Maturation / Estrogen Receptor / 窒素酸化物定量 / エストロゲン
Research Abstract

The endothelial type(NOS-3) of three isoforms of nitric oxide(NO) synthase occurs in porcine oocytes and granulose cells, but the regulation of NO synthesis in oocytes remains unknown. The present study was designed to evaluate steroid control in the process of oocyte NO synthesis. Cumulus-oocyte complexes(COCs), obtained from small-sized antral follicles of immature porcine ovaries, were cultured in estrogen-deprived medium, and the effect of steroids or steroid-free porcine follicular fluids on the NO release from oocytes was investigated. Oocytes that were isolated from cultured COCs were incubated with 1μM ionomycin. The NO metabolites were identified using a NO detector-high-pressure liquid chromatography system. Oocytes from COCs cultured with 10nM 17β-estradiol(E_2) released NO in response to ionomycin, whereas progesterone and testosterone had little effect on the synthesis of NO. An inhibitor of NOS suppressed the synthesis of NO. The maximal synthesis was observed after a 15 h-culture with E_2. However, oocytes freshly obtained from antral follicles did not response to ionomycin, and the E_2 action was suppressed by the addition of steroid-free follicular fluids. Analyses of RT-PCR and Western blotting showed that E_2 did not increase NOS-3 expression. In addition, estrogen receptor β was detected in oocytes and cumulus cells, and estrogen receptor a was detected only in cumulus cells. These findings suggest that oocyte NOS-3 is promoted for the synthesis of NO by E_2 without increases in NOS-3 expression, but the synthesis of NO is suppressed, at least in the oocytes of early antral follicles.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Hattori M-A, Arai M, Saruwatari K, Kato Y: "Estrogen regulation of nitirc oxide synthesis in the porcine oocyte."Molecular and Cellular Biochemistry. 260. 13-19 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujioka T, Soh T, Fujihara N, Hattori M-A: "Function of TGF-β2 in the growth of chicken primordial germ cells and geminal ridge stroma cells during embryonic development."The Journal of Experimental Zoology. 301(A). 290-296 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Xi YM, Nada Y, Soh T, Fujihara N, Hattori M-A: "Green fluorescent protein gene- transfected peafowl somatic cells participate in the development of chicken embryos."The Journal of Experimental Zoology. 301(A). 139-149 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sato F, Nakagawa T, Ito M, Kitagawa Y, Hattori M-A: "Application of RNA interference to chicken embryos using small interfering RNA."The Journal of Experimental Zoology. (印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okuno S, Aramaki S, Sato F, Soh T, Yamauchi N, Kato Y, Hattori M-A: "Expression of Cmdr1 in chicken embryonic gonads during sexual differentiation."The Journal of Experimental Zoology. (印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hattori M-A, Arai M, Saruwatari K, Kato Y: "Estrogen regulation of nitirc oxide synthesis in the porcine oocyte."Molecular and Cellular Biochemistry. 260(1). 13-19 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujioka T, Soh T, Fujihara N, Hattori M-A: "Function of TGF-β2 in the growth of chicken primordial germ cells and geminal ridge stroma cells during embryonic development."The Journal of Experimental Zoology. 301A(4). 290-296 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Xi YM, Nada Y, Soh T, Fujihara N, Hattori M-A: "Green fluorescent protein gene-transfected peafowl somatic cells participate in the development of chicken embryos."The Journal of Experimental Zoology. 301A(2). 139-149 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sato F, Nakagawa T, Ito M, Kitagawa Y, Hattori M-A: "Application of RNA interference to chicken embryos using small interfering RNA."The Journal of Experimental Zoology. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okuno S, Aramaki S, Sato F, Soh T, Yamauchi N, Kato Y, Hattori M-A: "Expression of Cmdrl in chicken embryonic gonads during sexual differentiation."The Journal of Experimental Zoology. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hattori M-A, Kato Y, Fujihara N: "Retinoic acid suppression of endothelial nitric oxide synthase in porcine oocytes."The Canadian Journal of Physiology and Pharmacology. 80. 777-782 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takesue K, Tabata S, Sato F, Hattori M-A: "Expression of functional nitric oxide synthase-3 in the porcine oocyte obtained from different developing stages of follicles."The Journal of Reproduction and Development. 49. 135-140 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Xi YM, Nada Y, Soh T, Fujihara N, Hattori M-A: "Establishment of feather follicle stem cells as potential vehicles for delivering exogenous genes in birds."The Journal of Reproduction and Development. 49. 213-219 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Xi YM, Nada Y, Soh T, Fujihara N, Hattori M-A: "Green fluorescent protein gene-transfected peafowl somatic cells participate in the development of chicken embryos."The Journal of Experimental Zoology. 301A(2). 139-149 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hattori M-A, Arai M, Saruwatari K, Kato Y: "Estrogen regulation of nitirc oxide synthesis in the porcine oocyte."Molecular and Cellular Biochemistry. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Fujioka T, Soh T, Fujihara N, Hattori M-A: "A possible function of transforming growth factor-β2 during the development of chicken embryonic gonad."The Journal of Experimental Zoology. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hattori M-A, Kato Y, Fujihara N: "Retinoic acid suppression of endothelial nitric oxide synthase in porcine oocytes"Can J Physiol Pharmacol. 80. 777-782 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takesue K, Tabata S, Sato F, Hattori M-A: "Expression of functional nitric oxide synthase-3 in the porcine oocyte obtained from different developing stages of follicles"J Reprod Dev. 49(in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tomizawa K, Ogawa S, Kato T, Watanabe M, Sato I, Hattori M-A: "Production and characterization of an antiserum against recombinant porcine follicle stimulating hormone"J Reprod Dev. 48. 131-136 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takesue K, Hattori M-A, Nishida N, Kato Y, Fujihara N: "Expression of endothelial nitric oxide synthase gene in cultured porcine granulosa cells after FSH stimulation"J Mol Endocrinol. 26. 259-265 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hattori M-A, Takesue K, Kato Y, Fujihara N: "Expression of endothelial nitric oxide synthase gene in the porcine oocyte and its possible function"Mol Cell Biochem. 219. 121-126 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takesue K, Nishida N, Hattori M-A, Kato Y, Fujihara N: "Stimulation of nitric oxide synthesis in porcine granulosa cells by phorbol ester"J Reprod Dev. 47. 1-6 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hattori M-A, Furuta H, Hiyama Y, Kato Y, Fujihara N: "Prominent expression of transforming growth factor b2 in the chicken embryonic gonad as revealed by suppressive subtraction cloning"Gen Comp Endocrinol. 124(in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hattori M-A, Fujioka H, Kato Y, Fujihara N: "Preferential expression of ADP-ribosylation factor gene in the chick embryonic gonads"Life Sci. 70. 1-7 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kato Y, Kato T, Tomizawa K, Ogawa S, Hattori M-A. Inoue K: "Presence of a 85 kDa pituitary protein (Pit-G) in porcine GH-producing cells"J Reprod Dev. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hattori M-A, Nishida N, Takesue, K, Kato Y, Fujihara N: "Synthesis of nitric oxide in the ovaries and its function. In "Reproductive Biotechnology""Miyamoto H, Manabe N (Hokuto Printing Company). 5 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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