• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Effect of diverse nitrogen-fixing bacterial community inhabiting the rhizoplane of acid sulfate soil-tolerant plants on nitrogen circulation around the rhizophere.

Research Project

Project/Area Number 13460143
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 生物資源科学
Research InstitutionHokkaido University

Principal Investigator

HASHIDOKO Yasuyuki  Hokkaido University, Hokkaido University, Graduate School of Agriculture, Associate Professor (40281795)

Co-Investigator(Kenkyū-buntansha) TAHARA Satoshi  Hokkaido University, Graduate School of Agriculture, Professor (50001475)
HASEGAWA Toshihiro  Hokkaido University, Graduate School of Agriculture, Associate Professor (10228455)
SHINANO Takuro  北海道大学, National Institute for Agro-Environmental Sciences, Group Leader (20235542)
FUKUSHI Yukiharu  Hokkaido University, Graduate School of Agriculture, Lecturer (60218906)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥14,400,000 (Direct Cost: ¥14,400,000)
Fiscal Year 2003: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 2001: ¥6,500,000 (Direct Cost: ¥6,500,000)
Keywordsacid sulfate soil-tolerant plants / rhizoplane bacteria / nitrogen fixing bacteria / nitrogen circulation / Sphingomonas sp. / rhizo biocomplex / functionality on the rhizoplane / nifgenes / 強酸性耐性 / 根面微生物 / 微生物フローラの多様性 / 単生窒素固定細菌 / 根面pHの上昇 / Sphingomonas / 根面バイオフィルム / 硫酸根の還元 / 微生物のフローラの多様性 / 耐酸性の獲得
Research Abstract

In this study, we cultured rot washings in nitrogen-free, soft gel medium as a primary screening of functional rhizoplane and/or rhizosphere microbes, and we efficiently and selectively obtained α- and β-proteobacteria to exclude saprophytic γ-proteobacteria. Those oligotrophic and/or diazotrophic bacteria were visualized their behaviors in the soft gel medium, including their biocomplex formation and tripartite partnerships. The bacterial flora propagated in the N-free soft gel medium of gellan gum base survived relatively longer in the medium at room temperature due to a biofilm-like colonization and quorum-sensing like dormancy. Thus, we were able to trail new area of functional rhizo-biocomplex connected to the study of behabior of rhizoplane microfloral communities. As a demonstration, we have isolated and sub-cultured two rhizoplane bacteria, Sphingomonas rosa and Burkholderia cepacia, from rhizoplane of Melastoma malabathricum, a representative acid sulfate soil-tolerant plant, and identified them with 16S rRNA gene sequencing and data base search. The S. rosa showed. abilities to alter rhizospherous conditions and also to enhance nitrogen supply from the rhizo-biocomplex in a sotrongly acidic conditions. Like S. rosa, many other Sphingomonas spp. often isolated from the rhizoplane of acid-tolerant plants in Kalimantan had characteristics of 1) contributing to reliving divert soil in the rhizosphere, 2) fixing nitrogen in the rhizoplane or at least adjusting conditions for nitrogen fixation, and 3) being regulated the microfloral population via Mg^<2+> in the rhizosphere. In addition, Sphingomonas spp. had tight partnerships and tripartite-like co-works in the rhizoplane and/or inner tissues of host plants. Sphingomonas spp. are thus expected to be a motive force for nitrogen circulation and high genetic diversity in the rhizo-biocomplex.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (22 results)

All 2004 2002 2001 Other

All Journal Article (12 results) Patent(Industrial Property Rights) (1 results) Publications (9 results)

  • [Journal Article] Cell growth restoring of rhizoplane bacterium Sphingomonas yanoikuyae EC-S001 from the rhizoplane of spinach (Spinacia oleracea) seedlings.2004

    • Author(s)
      Henny Hoo, Yasuyuki Hashidoko, Md.Tofazzal Istam, Satoshi Tahara.
    • Journal Title

      Applied and Environmental Microbiology 70

      Pages: 5214-5221

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Neutral rhizoplane pH of local rice and some predominant tree species : a possible strategy of plant adaptation to acidic-soil.2004

    • Author(s)
      Yasuyuki Hashidoko, Toshihiro Hasegawa, Erry Pumomo, Suwido H.Limin, Mitsuru Osaki, Satoshi Tahara
    • Journal Title

      Tropics 12(in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Cell growth restoring of rhizoplane bacterium Sphingomonas yanoikuyae EC-S001 from the rhizoplane of oleracea) seedlings. spinach (Spinacia oleracea) seedlings.2004

    • Author(s)
      Henny Hoo, Yasuyuki Hashidoko, Md.Tofazzal Islam, Satoshi Tahara.
    • Journal Title

      Applied and Environmental Microbiology 70(9)

      Pages: 5214-5221

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Neutral rhizoplane pH of local rice and some predominant tree species : a possible strategy of plant adaptation to acidic-soil.2004

    • Author(s)
      Yasuyuki Hashidoko, Toshihiro Hasegawa, Erry Purnomo, Suwido H.Limin, Mitsuru Osaki, Satoshi Tahara.
    • Journal Title

      Tropics 12(in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Soft gel medium solidified with gellan gum for preliminary screening for 的oot-associating, free-living nitrogen-fixing bacteria inhabiting the rhizoplane of plant2002

    • Author(s)
      Yasuyuki Hashidoko, Motohiko Tada, Mitsuru Osaki, Satoshi Tahara.
    • Journal Title

      Bioscience, Biotechnology and Biochemistry 66

      Pages: 2259-2263

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Characterization of five phyllosphere bacteria isolated from Rosa rugosa leaves, and their phenotypic and metabolic properties.2002

    • Author(s)
      Yasuyuki Hashidoko, Eriko Itoh, Kentaro Yokota, Tadashi Yoshida, Satoshi Tahara
    • Journal Title

      Bioscience, Biotechnology and Biochemistry 66

      Pages: 2474-2478

    • NAID

      110002693584

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Localization of flavonoids in the yellow lupin seedlings and their UV-B-absorbing potential.2002

    • Author(s)
      Yasufumi Katagiri, Yasuyuki Haskidoko, Satoshi Tahara.
    • Journal Title

      Zeitschrift fur Naturforschung 57c

      Pages: 811-816

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Soft gel medium solidified with gellan gum for preliminary screening for root-associating, free-living nitrogen-fixing bacteria inhabiting the rhizoplane of plants.2002

    • Author(s)
      Yasuyuki Hashidoko, Motohiko Tada, Mitsuru Osaki, Satoshi Tahara
    • Journal Title

      Bioscience, Biotechnology and Biochemistry 66(10)

      Pages: 2259-2263

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Characterization of five phyllosphere bacteria isolated from Rosa rugosa leaves, and their phenotypic and metabolic properties.2002

    • Author(s)
      Yasuyuki Hashidoko, Eriko Itoh, Kentaro Yokota, Tadashi Yoshida, Satoshi Tahara.
    • Journal Title

      Bioscience, Biotechnology and Biochemistry 66(11)

      Pages: 2474-2478

    • NAID

      110002693584

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Localization of flavonoids in the yellow lupin seedlings and their UV-B-absorbing potential.2002

    • Author(s)
      Yasufumi Katagiri, Yasuyuki Hashidoko, Satoshi Tahara.
    • Journal Title

      Zeitschrift fur Naturforschung 57c

      Pages: 811-816

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Penicillium fungi from Picea glehnii seeds protect the seedlings from damping-off.2001

    • Author(s)
      Keiko Yamaji, Yukiharu Fukushi, Yasuyuki Hashidoko, Tadashi Yoshida, Satoshi Tahara.
    • Journal Title

      New Phytologists 152

      Pages: 521-531

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Penicillium fungi from Picea glehnii seeds protect the seedlings from damping-off.2001

    • Author(s)
      Keiko Yamaji, Yukiharu Fukushi, Yasuyuki Hashidoko, Tadashi Yoshida, Satoshi Tahara
    • Journal Title

      New Phytologists 152

      Pages: 521-531

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 微生物保存用ゲル2004

    • Inventor(s)
      橋床泰之, 田原哲士, 大崎満
    • Industrial Property Rights Holder
      JIST
    • Filing Date
      2004-05-06
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Henny Hoo, Yasuyuki Hashidoko, Md.Tofazzal Islam, Satoshi Tahara: "Relatively high threshold level of Mg^<2+> cation required for cell growth of a rhizoplane bacterium Sphingomonas yanoikuyae EC-S001"Applied and Environmental Microbiology. (Accepted).

    • Related Report
      2003 Annual Research Report
  • [Publications] Yasuyuki Hashidoko, Yukako Gotou, Henny Hoo, Mitsuru Osaki, Erry Purnomo, Limin H.Suwido, Satoshi Tahara: "Role of nitrogen-fixing bacteria from Melastoma sp."Proceedings of International Sympodium on Land Management and Biodiversity in Southeast Asia. 135-138 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 橋床泰之: "高度負荷土壌での植物の生存戦略-根圏複合系の中で根面細菌が果たす機能-"化学と生物. 第41巻. 434-441 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 橋床泰之: "二次代謝産物を介した高等植物と着生微生物の相互作用研究"日本農芸化学会誌. 78巻. 1210-1217 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yasuyuki Hashidoko: "Soft gel medium solidified with gellan gum for preliminary screening for root-associating, free-living nitrogen-fixing bacteria inhabiting the rhizoplane of plants"Bioscience, Biotechnology and Bioscience. 66・10. 2256-2263 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yasuyuki Hashidoko: "Characterization of free-living nitrogen-fixing bacteria isolated from rhizoplane of Melastoma sp. inhabiting acidic plain land in Kalimantan and their ecological role"Tropics (Proceedings of TROPEAT 2002). (Accepted for publication). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mitsuru Osaki: "Novel technology of rhizosphere regulation to grow plants in adverse soil-a review"Plant and Soil. (Accepted for publication). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yasufumi Katagiri: "Localization of flavonoids in the yellow lupin seedlings and their UV-B-absorbing potential., 57c, 811-816 (2002)"Zeitschrift fur Naturforschung. 57c・9/10. 811-816 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Jun Wasaki: "Secreted acid phosphatase is expressed in cluster roots of Lupin in response to phosphorus deficiency"Plant and Soil. 248. 129-136 (2003)

    • Related Report
      2002 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi