• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Mechanisms underlying the integration of cell differentiation and proliferation by Id proteins

Research Project

Project/Area Number 13470034
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionFukui Medical University

Principal Investigator

YOKOTA Yoshifumi  Fukui Medical Univ., Faculty of Medicine, Professor, 医学部, 教授 (50222386)

Co-Investigator(Kenkyū-buntansha) MORI Seiichi  Fukui Medical Univ., Faculty of Medicine, Research Associate, 医学部, 助手 (10334814)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥14,600,000 (Direct Cost: ¥14,600,000)
Fiscal Year 2002: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 2001: ¥10,400,000 (Direct Cost: ¥10,400,000)
KeywordsId2 / differentiation / proliferation / KO mouse / behavioral anormaly / mammary gland / immune cells / 分化抑制因子 / bHLH因子 / 多動 / NK細胞 / 乳腺上皮細胞 / 増殖制御
Research Abstract

Id2 is a negative regulator of bHLH transcription factors and participate in the regulation of cell differentiation and proliferation. The aim of this project is to elucidate the mechanisms underlying the regulation of cell differentiation and proliferation through the analyses of phenotypes found in Id2-deficient mice. The phenotypes include behavioral anormaly, lactation defect and impaired differentiation of immune cells.
1. The central nervous system
We failed to detect histological alteration in the substantia nigra and striatum of Id2 KO mice. In situ hybridization analyses for neurogenic bHLH factors identified enhanced and widened expression of Math2 in the cerebral cortex of embryos on day 11.5, while other bHLH factors such as Mash1 and neurogenin2. At the same time point, immunohistochemistry using the Ki67 antibody revealed the decreased proliferation neural cells in the ventricular zone. In the adult, there was no significant alteration of the number and distribution of GABA … More ergic neurons in the substantia nigra and striatum. However, electrophysiological assays identified increased spontaneous firing rate of GABAergic neurons in the pars reticulata of the substantia nigra (1.3 fold), compared to that of the control littermates.
2. Mammary glands
To know an in vivo role of Id2 in cell cycle regulation, we crossed Id2 KO mice with other genetically modified mouse lines and analyzed whether the lactation defect in Id2 KO mice was rescued. However, cossing with transgenic mice bearing cyclin D1 under the control of the mouse mammary tumor virus (MMTV) promoter or p27 KO mice did not rescue the phenotype of Id2 KO mice.
3. The immune system
We found that Id2 KO mice lack the nasopharyngeal lymphoid tissue. In addition, we succeeded the reconstitution of the tissue by transferring lymphotoxin-expressing cells into Id2 KO mice. Furthermore, Id2 KO mice wererevealed to be in the Th2 dominant state due to a selective and prominent defect in CD8α+ dendritic cell subset. In B cells, Id2 was found to play an important role in suppressing the class switch to IgE. Less

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (39 results)

All Other

All Publications (39 results)

  • [Publications] Fujii, Y. et al.: "Taxon-specific ξ-crystallin in Japanese tree frog(Hyla japonica)lens"J. Biol. Chem.. 276. 28134-28139 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tachibana, M. et al.: "Genomic organization and chromosomal mapping of the basic helix-loop-helix factor OUT(Tcf23/TCF23)"Cytogenet. Cell Genet.. 94. 23-25 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yokota, Y. et al.: "In vivo function a differentiation inhibitor, Id2"IUBMB Life. 51. 207-214 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yokota, Y. et al.: "Id and development"Oncogene. 20. 8290-8298 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Greichmann, M. et al.: "Identification of inhibitor-of differentiation 2 as a modulator of neuronal apoptosis"J. Neurochem.. 80. 755-762 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Lasorella, A. et al.: "Id2 is critical for cellular proliferation and is the oncogenic effector of N-myc in human neuroblastoma"Cancer Res.. 62. 301-306 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fukuyama, S. et al.: "Initiation of NALT organogenesis is independent of the IL-7R, LTα1β2/LTβR and NIK signaling pathways but does require the Id2 gene and CD3-CD4+CD45+cells"Immunity. 17. 31-40 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yokota, Y., Mori, S.: "The role of Id family proteins in growth control"J. Cell. Physiol.. 190. 21-28 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kusunoki, T. et al.: "TH2 dominance and defective development of CD8+dendritic cell subset in Id2-deficient mice"J. Allergy Clin. Immunol.. 111. 136-142 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sugai, et al.: "Essential role of Id2 in negative regulation of IgE class switching"Nat. Immunool.. 4. 25-30 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hacker, C. et al.: "Transcriptional profiling identifies Id2 function in dendritic cell development"Nat. Immunol.. 4. 380-386 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fujii, Y. et al.: "Taxon-specific ζ-crystallin in Japanese tree frog (Hyla japonica) lens"J.Biol.Chem.. 276. 28134-28139 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tachibana, M. et al.: "Genomic organization and chromosomal mapping of the basic helix-loop-helix factor OUT (Tcf23/TCF23)"Cytogenet.Cell Genet.. 94. 23-25 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yokota, Y. et al.: "In vivo function of a differentiation inhibitor, Id2"IUBMB Life. 51. 207-214 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yokota, Y.: "Id and development"Oncogene. 20. 8290-8298 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Greichmann, M. et al.: "Identification of inhibitor-of-differentiation 2 as a modulator of neuronal apoptosis"J.Neurochem.. 80. 755-762 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Lasorella, A. et al.: "Id2 is critical for cellular proliferation and is the oncogenic effector of N-myc in human neuroblastoma"Cancer Res.. 62. 301-306 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fukuyaam, S. et al.: "Initiation of NALT organogenesis is independent of the IL-7R, LTα1β2/LTβR and NIK signaling pathways but does require the Id2 gene and CD3-CD4+CD45+ cells"Immunity. 17. 31-40 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yokota, Y. and Mori, S.: "The role of Id family proteins in growth control"J.Cell.Physiol.. 190. 21-28 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kusunoki, T. et al.: "TH2 dominance and defective development of CD8+ dendritic cell subset in Id2-deficient mice"J.Allergy Clin.Immunol.. 111. 136-142 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sugai, et al.: "Essential role of Id2 in negative regulation of IgE class switching"Nat.Immunol.. 4. 25-30 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hacker, C. et al.: "Transcriptional profiling identifies Id2 function in dendritic cell development"Nat.Immunol.. 10. 380-386 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fujii, Y., et al.: "Taxon-specific ζ-crystallin in Japanese tree frog (Hyla japonica) lens"J.Biol.Chem.. 276. 28134-28139 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tachibana, M., et al.: "Genomic organization and chromosomal mapping of the basic helix-loop-helix factor OUT (Tef23/TCF23)"Cytogenet.Cell Genet. 94. 23-25 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yokota, Y., et al.: "In vivo function of a differentiation inhibitor, Id2"IUBMB Life. 51. 207-214 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yokota, Y., et al.: "Id and development"Oncogene. 20. 8290-8298 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Greichmann, M., et al.: "Identification of inhibitor-of-differentiation 2 as a modulator of neuronal apoptosis"J.Neurochem.. 80. 755-762 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Lasorella, A., et al.: "Id2 is critical for cellular proliferation and is the oncogenic effector of N-myc in human neuroblastoma"Cancer Res.. 62. 301-306 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Fukuyama, S., et al.: "Initiation of NALT organogenesis is independent of the IL-7R, LTα1β2/LTβR and NIK signaling pathways but does require the Id2 gene and CD3-CD4+CD45+ cells"Immunity. 17. 31-40 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yokota, Y., Mori, S.: "The role of Id family proteins in growth control"J.Cell.Physiol.. 190. 21-28 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kusunoki, T., et al.: "TH2 dominance and defective development of -CD8+ dendritic cell subset in Id-2-deficient mice"J.Allergy Clin.Immunol.. 111. 136-142 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sugai, et al.: "Essential role of Id2 in negative regulation of IgE class switching"Nat.Immunool.. 4. 25-30 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hacker, C., et al.: "Transcriptional profiling identifies Id2 function in dendritic cell development"Nat.Immunol.. 4. 380-386 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] FUjii, Y.: "Taxon-specific ζ-crystallin in Japanese tree frog (Hyla japonica) lens"J.Biolo.Chem.. 276(30). 28134-28139 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tachibana, A.: "Genomic organization and chromosomal mapping of the basic helix-loop-helix factor OUT(Tcf23/TCF23)"Cytogenet. Cell Genet.. 94(1-2). 23-25 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Greichmann, M.: "Identification of inhibitor-of-differentiation 2 as a modulator of neuronal apoptosis"J. Neurochem.. 80. 755-762 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Lasorella, A.: "Id2 is critical for cellular proliferation and is the oncogenic effector of N-myc in human neuroblastoma"Cancer Research. 62(1). 301-306 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yokota, Y.: "In vivo function of a differentiation inhibitor, Id2"IUBMB Life. 51(4). 207-214 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yokota, Y.: "Id and development"Oncogene. 20(58). 8290-8298 (2001)

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi