Isolation and characterization of stem cell-derived neural stem/progenitor cell supporting factor, SDNSF.
| Project/Area Number |
13470036
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
TASHIRO Kei Kyoto University, Center for Molecular Biology and Genetics, Assistant Professor, 遺伝子実験施設, 助教授 (10263097)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Jun Kyoto University, Graduate School of Medicine, Research Associate, 医学研究科, 助手 (10270779)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥14,400,000 (Direct Cost: ¥14,400,000)
Fiscal Year 2002: ¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 2001: ¥7,500,000 (Direct Cost: ¥7,500,000)
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| Keywords | stem cell / inner ear / secreted molecule / signal-sequence trap / otocyst / soluble factor / rat / development / 生存支持因子 / 幹細胞由来神経生存因子 / SDNSF / 分泌タンパク / カルシウム依存性 / 遺伝子欠損マウス / 細胞生存支持活性 / MTTアッセイ / タンパク精製 / ポリクローナル抗体 / 細胞増殖因子 |
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| Research Abstract |
Recent evidences suggests that adult neural stem/progenitor cells (ANSCs) secrete autocrine/paracrine factors and that these intrinsic factors are involved in the maintenance of adult neurogenesis. We identified a novel secretory molecule, stem cell-derived neural stem/progenitor cell supporting factor (SDNSF), from adult hippocampal neural stem/progenitor cell s by using the signal sequence trap method. The expression of SDNSF in adult central nervous system was localized to hippocampus including dentate gyrus, where the neurogenesis persists throughout life. In induced neurogenesis status seen in ischemically treated hippocampus, the expression of SDNSF was up-regulated. As functional aspects, SDNSF protein provided a dose-dependent survival effect for ANSC following basic fibroblast growth factor 2 (FGF-2) withdrawal. ANSCs treated by SDNSF also retain self-renewal potential and multipotency in the absence of FGF-2. However, SDNSF did not have mitogenic activity, nor was it a cofactor that promoted the mitogenic effects of FGF-2. These data suggested an important role of SDNSF as an autocrine/paracrine factor in maintaining stem cell potential and lifelong neurogenesis in adult central nervous system.
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Report
(3 results)
Research Products
(9 results)
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[Publications] Shinohara, M.T., Ogura, A., Ikegawa, M., Inoue, K., Ogonuki, N., Tashiro K., Toyokuni, S., Honjo, T., Shinohara., T.: "Adenovirus-mediated gene delivery and in vitro microinsemination produce offspring from infertile male mice"Proc. Natl. Acad. Sci. U.S.A.. 99. 1383-1388 (2002)
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[Publications] Nakamura, T., Ruiz-Lozano, P., Ikeda, Y., Iwanaga, I., Hinek, A., Minamisawa, S., Chen.C.-F., Kobuke, K., Nancy.D., Takada, Y., Tashiro K., Ross, J., Honjo, T., Chien, R.K.: "Fibulin-5/DANCE is essential for elastrogenesis in vivo"Nature. 415. 171-175 (2002)
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[Publications] Kobuke, K., Furukawa, Y., Sugai, M., Tanigaki, K., Ohashi, N, Matsumori, A., Sasayama, S., Honjo, T., Tashiro, K.: "ESDN, a novel neuropilin-like membrane protein cloned from vascular cells with the longest secretory signal sequence among eukaryotes is upregulated after vascular injury"J. Biol. Chem.. 276. 34105-34114 (2001)
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[Publications] Kinoshita, M., Nakamura, T., Ihara, M., Haraguchi, T., Hiraoka, Y., Tashiro, K., Noda, M.: "Identification of human endomucin-1, and -2 as membrane-bound sialoglycoproteins eith anti-adhesive activity"FEBS Lett.. 499. 121-126 (2001)
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[Publications] Hattori, K., Rafii, S., Heissig, B., Tashiro, K., Honjo, T., Takeno, M., Shieh, J-H.Hackett, N.R., Crystal, R.G., Moore, M.S.: "Plasma elevation of stromal derived factor-1 induces mobilization of mature and immature hemacopoietic progenitor and stem cells."Blood. 97. 3354-3360 (2001)
