| Project/Area Number |
13470044
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
AKAGI Tadaatsu OKAYAMA UNIVERSITY, Graduate School of Medicine and Dentistry, Professor, 大学院・医歯学総合研究科, 教授 (20136386)
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| Co-Investigator(Kenkyū-buntansha) |
KONDO Eisaku OKAYAMA UNIVERSITY, Graduate School of Medicine and Dentistry, Assistant Professor, 大学院・医歯学総合研究科, 講師 (30252951)
OKA Takashi OKAYAMA UNIVERSITY, Graduate School of Medicine and Dentistry, Research Associate, 大学院・医歯学総合研究科, 助手 (50160651)
YOSHINO Tadashi OKAYAMA UNIVERSITY, Graduate School of Medicine and Dentistry, Professor, 大学院・医歯学総合研究科, 教授 (70183704)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥12,200,000 (Direct Cost: ¥12,200,000)
Fiscal Year 2002: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2001: ¥9,100,000 (Direct Cost: ¥9,100,000)
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| Keywords | Malignant lymphoma / MALT lymphoma / Multistep carcinogenesis / Helicobacter pylori / API2 / MALT1 / Heat shock protein 60 / Adhesion molecules / t(11;18) |
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| Research Abstract |
1Eppression of adhesion molecules was investigated in MALT lymphomas of the G-I tract, ocular adnexa, and thyroid. α4s7 integrin and L-selectin were detected on low-grade MALT lymphoma cells and low-grade portions of high-grade MALT lymphoma, but not on high-grade portions of high-grade MALT lymphoma or diffuse large B-cell lymphomas. Most MALT lymphoma cases of the ocular adnexa were positive only for L-selectin. Neither was detected in the thyroid. MAdCAM-1, a ligand for α4s7 integrin, was expressed on the HEV in the inflammatory lesions and MALT lymphoma tissues of theG-I tract and thyroid, but not in the ocular adnexa or salivary gland.
2.t(11;18)(API2/MALT1) in MALT lymphomas was investigated using FISH and RT-PCR. API2/MALT1 translocation was detected in a small portion of ocular adnexal and large intestinal MALT lymphomas and also rarely in diffuse large B-cell lymphomas and reactive lymphoreticular hyperplasia, but not in MALT lymphomas of the salivary gland and thyroid.
3.Eradication of Helicobacter pylori induced complete remission in 88% of the patients with gastric MALT lymphoma, but not effective in the PI2/MALT1-positive cases.
4.Clinicopathological and immunogenetic features of ocular adnexal lymphoproliferative disorders including MALT lymphomas were characterized.
5. Clinicopathological features of multiple organ MALT lymphomas were characterized.
6. Peripheral blood mononuclear cells in patients with MALT lymphoma showed increased expression of CD40 ligand and augmented production of IL4 by stimulation with cytokine or combined stimulation with HSP60. DNA microarray analysis indicated increased levels of HLA-DR and integrin mRNAs.
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