| Project/Area Number |
13470047
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Hokkaido University |
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Principal Investigator |
UEDE Toshimitsu Hokkaido Univ., Inst. For Genetic Med., Prof., 遺伝子病制御研究所, 教授 (00160185)
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| Co-Investigator(Kenkyū-buntansha) |
KON Shigeyuki Hokkaido Univ., Inst. For Genetic Med., Inst., 遺伝子病制御研究所, 助手 (90344499)
猪部 学 北海道大学, 遺伝子病制御研究所, 助手 (10312414)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥14,200,000 (Direct Cost: ¥14,200,000)
Fiscal Year 2003: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2002: ¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 2001: ¥6,900,000 (Direct Cost: ¥6,900,000)
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| Keywords | Thymocyte / Apoptosis / Transgenic mice / steroid / caspase |
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| Research Abstract |
1. Trangenic mice (TG) was established in which TDAG8 gene responsible for glucocorticoid-induced apoptosis was over-expressed.
2. The expression of mRNA including FasL, FADD, TRAIL, TNF-R and TRADD did not differ between TG and control mice upon glucocorticoid treatment.
3. TG mice exhibited augmented thymocyte apoptosis induced by glucocorticoid as compared to control mice.
4. Upon glucocorticoid treatment, activity of caspase 3, 8, and 9 was significantly augmented in TG mice as compared to control mice.
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