| Project/Area Number |
13470080
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hygiene
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| Research Institution | University of Fukui (2004)
福井医科大学 (2001-2003) |
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Principal Investigator |
KUSAKA Yukinori University of Fukui, Division of Environmental Health Department of International Social Medicine School of Medicine, Professor, 医学部, 教授 (70135680)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Kazuhiro University of Fukui, School of Medicine, Assistant professor, 医学部, 助教授 (40262620)
SUGANUMA Narufumi University of Fukui, School of Medicine, Lecturer, 医学部, 講師 (50313747)
MORI Tomio Fukui Prefectural Institute of Public Health and Environmental Science, Department of Life Science, Chief Researcher, 生活科学部, 主任研究員
MURAOKA Michio Fukui Prefectural Institute of Public Health and Environmental Science, Department of Life Science, Chief Researcher, 生活科学部, 主任研究員
櫻澤 博文 福井大学, 医学部, 助手 (50345675)
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| Project Period (FY) |
2001 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Metal / ultrafine powder / nickel / cobalt / leukocyte / interleukin / tumor necrosis factor / monocyte / 超微細金属粒子 / ヒト白血球 / サイトカイン / 鉄 / 金属超微細粒子 / TIF-α / NF-κB / ELISA / in-vitro実験系 / 活性化 |
|---|
| Research Abstract |
Activation of leukocytes was studied in leukocytes from ultrafine-nickel instilled rat and in leukocytes from healthy volunteers. In the former, at seven days after rats were instilled with Ut-Ni, peripheral leukocytes were recovered and cultured with Ut-Ni. NO and tumor necrosis factor-alpha were highly released from leukocytes. In the latter, peripheral leukocytes recovered from healthy volunteers and separated into monocyte and polymorph nuclear subsets. The two subsets were cultured with Ut-Ni, Ut-Co, and Ut-Fe. Ut-Ni caused release interleukin 6 and 8, and TNF-alpha from the monocyte subset most highly among the three metals. On the other hand, Ut-Fe caused IL-1 most highly from the mononuclear subset. Ut-Ni did no release of cytokines from the polumorphonuclear subset. Taken together, respiratory exposure to Ut-Ni was suggested to cause activation of peripheral leukocytes. In human, if exposure to Ut-Ni of peripheral monocytes occur, activation may follow.
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