| Project/Area Number |
13470128
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Nagasaki University |
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Principal Investigator |
NAGATAKE Tsuyoshi Nagasaki University, Institute of Tropical Medicine, Professor, 熱帯医学研究所, 教授 (30164445)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Kiwao Nagasaki University, Institute of Tropical Medicine, Assistant Professor, 熱帯医学研究所, 助手 (20220874)
WATANABE Hiroshi Nagasaki University, School of Medicine Hospital and Clinics, Lecturer, 医学部付属病院, 講師 (90295080)
OISHI Kazunori Nagasaki University, Institute of Tropical Medicine, Associate Professor, 熱帯医学研究所, 助教授 (80160414)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 2002: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2001: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | resoiratory infection / mechanism of pathoaenesis / prevention of infections / respiratory pathogenic bacteria / receptor / gangliocid / adherence to epithelial cells |
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| Research Abstract |
Streptococcus pneumoniae causes respiratory and other invasive infections. Increased esistance of this bacterium to antibiotics necessitates new approaches to the treatment of infections. Attachment of bacteria to human pharyngeal epithelial cells is the initial step in the pathogenesis of infection and S-carboxymethylcysteine (S-CMC) can modulate the attachment of Moraxella catarrhalis and nontypable Haemophilus influenzae to ーepithelial cells. Unlike these two, S. pneumoniae is gram-positive and has a well-defined capsule. Here we examined the effects of S-CMC on the attachment and detachment of S. pneumoniae to human pharyngeal epithelial cells in vitro. Treatment of these cells with S-CMC significantly reduced the number of attached S pneumoniae. S-CMC also resulted in a significant increase in the detachment of already attached S pneumoniae to epithelial cells. In addition, treatment of S. pneumoniae with S-CMC significantly reduced their ability to attach to epithelial cells, but not the number of viable bacteria. Our study shows that S-CMC modulates the attachment of S. pneumoniae to human pharyngeal epithelial cells by acting both on cells and bacteria.
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