| Project/Area Number |
13470151
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Keio University |
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Principal Investigator |
FUKUDA Keiichi Keio University, School of Medicine, Assistant professor, 医学部, 講師 (20199227)
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| Co-Investigator(Kenkyū-buntansha) |
ITABASHI Yuji Keio University, School of Medicine, Assistant, 医学部, 助手 (00317108)
MIYOSHI Shunichiro Keio University, School of Medicine, Assistant, 医学部, 助手 (10296577)
FUJITA Jun Keio University, School of Medicine, Assistant, 医学部, 助手 (10306706)
YAGI Takashi Keio University, School of Medicine, Assistant, 医学部, 助手 (40317136)
田原 聡子 慶應義塾大学, 医学部, 助手 (80296566)
富田 雄一 (冨田 雄一) 慶應義塾大学, 医学部, 助手 (00296568)
伯野 大彦 慶應義塾大学, 医学部, 助手 (80286476)
佐藤 敏彦 慶應義塾大学, 医学部, 助手 (40286464)
高橋 栄一 慶應義塾大学, 医学部, 助手 (00276247)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥16,400,000 (Direct Cost: ¥16,400,000)
Fiscal Year 2003: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 2002: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 2001: ¥6,800,000 (Direct Cost: ¥6,800,000)
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| Keywords | cardiomyocytes / Stem cell / Bone Marrow mesenchymal stem cell / Cell transplantation therapy / Differentiation / CD antigen / Regenerative medicine / 骨髄 / 表面抗原 / FACS / 多分化能 / 多能性幹細胞 / 心不全 / 発生分化 / 間葉系幹細胞 / 細胞増殖因子 / サイトカイン |
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| Research Abstract |
In order to obtain regenerative cardiomyocyte from bone marrow, we had developed a method to isolate mesenchymal stem cell from bone marrow. Using autoMACS, CD45-, CD29+, CD34-, CD44+ cells were isolated from fresh whole bone marrow cells, and were seeded on the fibronectin-coated dishes. FACS analysis revealed that these cells also showed CD 13-, and CD49e+. These cells could proliferate in culture, and differentiated into the adipocytes under the medium containing insulin. Moreover, these cells also differentiated into osteoblast under the condition of dexamethazone, ascorbic acid, and β-glyceroposphates. IGF-1, FGF, TGF-b, LIF, EGF, nor endotelin-1 did not augment cardimoyocyte differentiation in these cells. However, Wnt11 augmented cardiomyocyte differentiation in these cells.
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