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Establishment of a method to induce cardiomyocyte differetiation from bone marrow mesenchymal stem cell

Research Project

Project/Area Number 13470151
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionKeio University

Principal Investigator

FUKUDA Keiichi  Keio University, School of Medicine, Assistant professor, 医学部, 講師 (20199227)

Co-Investigator(Kenkyū-buntansha) ITABASHI Yuji  Keio University, School of Medicine, Assistant, 医学部, 助手 (00317108)
MIYOSHI Shunichiro  Keio University, School of Medicine, Assistant, 医学部, 助手 (10296577)
FUJITA Jun  Keio University, School of Medicine, Assistant, 医学部, 助手 (10306706)
YAGI Takashi  Keio University, School of Medicine, Assistant, 医学部, 助手 (40317136)
田原 聡子  慶應義塾大学, 医学部, 助手 (80296566)
富田 雄一 (冨田 雄一)  慶應義塾大学, 医学部, 助手 (00296568)
伯野 大彦  慶應義塾大学, 医学部, 助手 (80286476)
佐藤 敏彦  慶應義塾大学, 医学部, 助手 (40286464)
高橋 栄一  慶應義塾大学, 医学部, 助手 (00276247)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥16,400,000 (Direct Cost: ¥16,400,000)
Fiscal Year 2003: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 2002: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 2001: ¥6,800,000 (Direct Cost: ¥6,800,000)
Keywordscardiomyocytes / Stem cell / Bone Marrow mesenchymal stem cell / Cell transplantation therapy / Differentiation / CD antigen / Regenerative medicine / 骨髄 / 表面抗原 / FACS / 多分化能 / 多能性幹細胞 / 心不全 / 発生分化 / 間葉系幹細胞 / 細胞増殖因子 / サイトカイン
Research Abstract

In order to obtain regenerative cardiomyocyte from bone marrow, we had developed a method to isolate mesenchymal stem cell from bone marrow. Using autoMACS, CD45-, CD29+, CD34-, CD44+ cells were isolated from fresh whole bone marrow cells, and were seeded on the fibronectin-coated dishes. FACS analysis revealed that these cells also showed CD 13-, and CD49e+. These cells could proliferate in culture, and differentiated into the adipocytes under the medium containing insulin. Moreover, these cells also differentiated into osteoblast under the condition of dexamethazone, ascorbic acid, and β-glyceroposphates. IGF-1, FGF, TGF-b, LIF, EGF, nor endotelin-1 did not augment cardimoyocyte differentiation in these cells. However, Wnt11 augmented cardiomyocyte differentiation in these cells.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (62 results)

All Other

All Publications (62 results)

  • [Publications] Keiichi Fukuda: "Use of adult mesenchymal stem cells for regeneration of cardiomyocyte and its application to cell transplantation therapy"Bone Marrow Transplant. 32. S25-S27 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keiichi Fukuda: "Regeneration of cardiomyocytes from bone marrow : Use of mesenchymal stem cell for cardiovascular tissue engineering"Cytotechnology. 41. 165-175 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keiichi Fukuda: "Reprogramming of bone marrow mesenchymal stem cells into cardiomyocytes"Competes Rendus Biologies. 325. 1-12 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Daihiko Hakuno, Keiichi Fukuda, et al.: "Bone marrow-derived cardiomyocytes (CMG cell) expressed functionally active adrenergic and muscarinic receptors"Circulation. 105. 380-386 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keiichi Fukuda: "Development of regenerative cardiomyocytes from mesenchymal stem cells for cardiovascular tissue engineering"Artificial Organs. 25. 183-193 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Satoko Tahara, Keiichi Fukuda, et al.: "Potassium Channel Blocker Activates Extracellular Signal Regulated Kinases via Pyk2 and EGF Receptor in Rat Cardiomyocyes K^+ Channel Blocker-Evoked Signals in Cardiomyocyte"JAM Coll Cardiol.. 38. 1554-1563 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Motoaki Sano, Keiichi Fukuda, et al.: "ERK and p37MAPK, but not NF-κB, are critically involved in reactive oxygen species-mediated induction of IL-6 by angiotensin II in cardiac fibroblasts."Circ Res. 89. 661-669 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hiroaki Kodama, Keiichi Fukuda, et al.: "Role of EGF receptor and Pyk2 in endothelin-1-induced ERK activation in rat cardiomyocytes."J.Mol.Cell.Cardiol.. 34. 139-150 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keiichi Fukuda: "Molecular characterization of regenerated cardiomyocytes derived from adult mesenchymal stem cells."Congenital anomalies. 42. 1-9 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keiichi Fukuda: "Stem cell transplantation as a mode of regenerative medicine."Jpn Med Assocation J.. 46. 333-338 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hiroaki Kodama, Keiichi Fukuda, et al.: "Selective involvement of p130Cas/Crk/Pyk2/c-Src in endothelin-1-induced JNK activation"Hypertention. 41. 1372-1379 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ariizumi T, Fukuda K, et al.: "Amphibian in vitro heart induction : a simple and reliable model for the study of vertebrate cardiac development"Int J Dev Biol.. 47. 405-410 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shinsuke Yuasa, Keiichi Fukuda, et al.: "Cardiomyocytes undergo cells division following myocardial infarction is a spatially and temporally restricted event in rats."Mol Cell Biochem. 259. 177-181 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yasuyo Hisaka, Keiichi Fukuda, et al.: "Powerful and controllable angiogenesis by using gene-modified cells expressing human hepatocyte growth factor and thymidine kinase."J Am Coll Cardiol. 43. 1915-1922 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Masaki Ieda, Keiichi Fukuda, et al.: "Endothelin-1 regulates cardiac sympathetic nerve innervation in the rodent heart by controlling nerve growth factor expression."J Clin Invest. 113. 1-10 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Eiichi Takahashi, Keiichi Fukuda, et al.: "LIF activates cardiac L-type Ca^<2+> channels via phosphorylation of serine 1829 of rabbit Cav1.2"Cire Res. 94. 1242-1248 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keiichi Fukuda: "Regenerative medicine for cardiomyocyte."Jap Med Ass J.. 46. 333-338 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keiichi Fukuda: "Application of mesenchymal stem cells for the regeneration of cardiomyocyte and its use for cell transplantation therapy."Human Cell. 13. 83-94 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keiichi Fukuda: "Generation of regenerated cardiomyocytes and its therapeutic use for cell transplantation into failing heart."JAACT 2000. (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keiichi Fukuda: "Mesenchymal Stem Cells : Biology and Potential Clinical Use"Ministerio De Sanidad Y Consumo. (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keiichi Fukuda: "Cardiovascular genomics : New pathophysiological concepts"Pieter Doevendans, Stefan Kaab. Kluwer Academic Publishers(The Netherlands). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fukuda K: "Stem Cell Therapy for Autoimmune Disease(edited by Richard K.Burt and Alberto Marmont)"Landes Bioscience, USA. (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keiichi Fukuda: "Development of regenerative cardiomyocytes from mesenchymal stem cells for cardiovascular tissue engineering"Artificial Organs. 25. 183-193 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Satoko Tahara, Keiichi Fukuda, Hiroaki Kodama, Takahiro Kato, Shunichiro Miyoshi, Yuichi Tomita, Satoshi Ogawa: "Potassium Channel Blocker Activates Extracellular Signal Regulated Kinases via Pyk2 and EGF Receptor in Rat Cardiomyocytes K^+ Channel Blocker-Evoked Signals in Cardiomyocyte"JAM Coll Cardiol. 38. 1554-1563 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Motoaki Sano, Keiichi Fukuda, Takahiro Kato, Toshihiko Sato, Satoko Tahara, Makoto Suematsu, Satoshi Matsuda, Shigeo Koyasu, Hideo Matsui, Keiko Yamauchi-Takihara, Satoshi Ogawa: "ERK and p38MAPK, but not NF-κB, are critically involved in reactive oxygen species-mediated induction of IL-6 by angiotensin II in cardiac fibroblasts."Circ Res. 89. 661-669 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Daihiko Hakuno, Keiichi Fukuda, Shinji Makino, Fusako Konishi, Yuichi Tomita, Tomohiro Manabe, Yusuke Suzuki, Yasuyo Hisaka, Akihiro Umezawa, Satoshi Ogawa: "Bone marrow-derived cardiomyocytes (CMG cell) expressed functionally active adrenergic and muscarinic receptors."Circulation. 105. 380-386 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hiroaki Kodama, Keiichi Fukuda, Toshiyuki Takahashi, Motoaki Sano, Takahiro Kato, Satoko Tahara, Daihiko Hakuno, Toshihiko Sato, Tomohiro Manabe, Fusako Konishi, Satoshi Ogawa: "Role of EGF receptor and Pyk2 in endothelin-1-induced ERK activation in rat cardiomyocytes."J.Mol.Cell.Cardiol.. 34. 139-150 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keiichi Fukuda: "Molecular characterization of regenerated cardiomyocytes derived from adult mesenchymal stem cells."Congenital anomalies. 42. 1-9 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keiichi Fukuda: "Reprogramming of bone marrow mesenchymal stem cells into cardiomyocytes."Competes Rendus Biologies. 325. 1-12 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keiichi Fukuda: "Use of adult mesenchymal stem cells for regeneration of cardiomyocyte and its application to cell transplantation therapy."Bone Marrow Transplant. 32. S25-S27 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keiichi Fukuda: "Stem cell transplantation as a mode of regenerative medicine."Jpn Med Association J.. 46. 333-338 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hiroaki Kodama, Keiichi Fukuda, Eiichi Takahashi, Satoko Tahara, Yuichi Tomita, Masaki Ieda, Kensuke Kimura, Koji M.Owada, Kristiina Vuori, Satoshi Ogawa: "Selective involvement of p130Cas/Crk/Pyk2/c-Src in endothelin-1-induced JNK activation."Hypertention. 41. 1372-1379 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ariizumi T, Kinoshita M, Yokota C, Takano K, Fukuda K, Moriyama N, Malacinski GM, Asashima M.: "Amphibian in vitro heart induction a simple and reliable model for the study of vertebrate cardiac development"Int J Dev Biol. 47. 405-410 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keiichi Fukuda: "Regeneration of cardiomyocytes from bone marrow : Use of mesenchymal stem cell for cardiovascular tissue engineering."Cytotechnology. 41. 165-175 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shinsuke Yuasa, Keiichi Fukuda, Yuichi Tomita, Jun Fujita, Masaki Ieda, Satoko Tahara, Yuji Itabashi, Takashi Yagi, Haruko Kawaguchi, Yasuyo Hisaka, Satoshi Ogawa: "Cardiomyocytes undergo cells division following myocardial infarction is a spatially and temporally restricted event in rats."Mol Cell Biochem. 259. 177-181 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yasuyo Hisaka, Keiichi Fukuda, Masaki Ieda, Kensuke Kimura, Isao Shibuya, Haruko Kawaguchi, Toshikazu Nakamura, Hidezo Mori, Koji Kimura, Naoto Fukuyama, Kenichiro Kosai, Satoshi Ogawa: "Powerful and controllable angiogenesis by using gene-modified cells expressing human hepatocyte growth factor and thymidine kinase."J Am Coll Cardiol. 43. 1915-1922 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Masaki Ieda, Keiichi Fukuda, Kensuke Kimura, Yasuyo Hisaka, Haruko Kawaguchi, Kouji Shimoda, Eiko Takeshita, Hideyuki Okano, Yukiko Kurihara, Hiroki Kurihara, Junji Ishida, Akiyoshi Fukamizu, Linda Salamone, Howard J.Federoff, Satoshi Ogawa: "Endothelin-1 regulates cardiac sympathetic nerve innervation in the rodent heart by controlling nerve growth factor expression."J Clin Invest. 113. 1-10 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Eiichi Takahashi, Keiichi Fukuda, Shunichiro Miyoshi, Mitsushige Murata, Takahiro Kato, Makoto Ita, Tsutomu Tanabe, Satoshi Ogawa.: "LIF activates cardiac L-type Ca^<2+> channels via phosphorylation of serine 1829 of rabbit Cav1.2"Circ Res. 94. 1242-1248 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keiichi Fukuda: "Regenerative medicine for cardiomyocyte."Jap Med Ass J. 46. 333-338 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keiichi Fukuda: "Application of mesenchymal stem cells for the regeneration of cardiomyocyte and its use for cell transplantation therapy."Human Cell. 13. 83-94 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keiichi Fukuda: "Generation of regenerated cardiomyocytes and its therapeutic use for cell transplantation into failing heart."JAACT 2000. (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keiichi Fukuda: "Use of mesenchymal stem cells for regeneration of cardiomyocytes and its application to the treatment of congestive heart failure."Cardiovascular genomics : New pathophysiological concepts (Pieter Doevendans, Stefan Kaab) (Kluwer Academic Publishers(The Netherlands)). 245-256 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keiichi Fukuda: "Regeneration of cardiomyocytes from bone marrow stem cells and its application to cell transplantation therapy"Mesenchymal Stern Cells : Biology and Potential Clinical Use. (Santiagi Grisolia, M. Dolores Minana, Elena Bendala-Tufanisco, Edit.) (Ministerio De Sanidad Y Consumo(Madrid, Spain)). 121-145 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fukuda K: "Regeneration of cardiomyocytes from bone marrow stem cells and application to cell transplantation therapy."Stem Cell Therapy for Autoimmune Disease (edited by Richard K.Burt and Alberto Marmont) (Landes Bioscience, USA). 39-49 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keiichi Fukuda: "Use of adult mesenchymal stem cells for regeneration of cardiomyocyte and its application to cell transplantation therapy"Bone Marrow Transplant. 32. S25-S27 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Keiichi Fukuda: "Regeneration of cardiomyocytes from bone marrow : Use of mesenchymal stem cell for cardiovascular tissue engineering."Cytotechnology. 41. 165-175 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] S Yuasa, K Fukuda, et al.: "Cardiomyocytes undergo cells division following myocardial infarction is a spatially and temporally restricted event in rats"Mol Cell Biochem. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] H Kodama, K Fukuda, et al.: "Selective involvement of p13OCas/Crk/Pyk2/c-Src in endothelin-1-induced JNK activation"Hypertention. 41. 1372-1379 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ariizumi T, Fukuda K, et al.: "Amphibian in vitro heart induction : a simple and reliable model for the study of vertebrate cardiac development"Int J Dev Biol. 47. 405-410 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Keiichi Fukuda: "Application of mesenchymal stem cells for the regeneration of cardiomyocyte and its use for cell transplantation therapy"Human Cell. 13. 83-94 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Keiichi Fukuda: "Mesenchymal Stem Cells : Biology and Potential Clinical Use"Ministerio De Sanidad Y Consumo. (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] H Kodama, K Fukuda, et al.: "Role of EGF receptor and Pyk2 in endothelin-1-induced ERK activation in rat cardiomyocytes"J. Mol. Cell. Cardiol.. 34. 139-150 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Keiichi Fukuda: "Molecular characterizaiion of regenerated cardiomyocytes derived from adult mesenchymal stem cells"Congenital anomalies.. 42. 1-9 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] D Hakuno, K Fukuda, et al.: "Bone marrow-derived cardiomyocytes expressed functionally active adrenergic and muscarinic receptors"Circulation. 105. 380-386 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Keiichi Fukuda: "Reprogramming of bone marrow mesenchymal stem cells into cardiomyocytes"Competes Rendus Biologies. 325. 1-12 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Keiichi Fukuda: "Cardiovascular genomics: New pathophysiological"Kluwer Academic Publishers. 275 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hiroaki Kodama, Keiichi Fukuda et al.: "Role of EGF receptor and Pyk2 in endothelin-1-induced ERK activation in rat cardiomyocytes"J.Mol.Cell.Cardiol.. 34. 139-150 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] keiichi Fukuda: "Molecular characterization of regenerated cardiomyocytes derived from adult mesenchymal stem cells"Congenital anomaltes. 42. 1-9 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] keiichi Fukuda: "Development of regenerative cardiomyocytes from mesenchymal stem cells for cardiovascular tissue engineering"Artificial Organs. 25. 183-193 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] keiichi Fukuda: "Potassium Channel Blocker Activates Extracellular Signal Regulated Kinases via Pyk2 and EGF Receptor in Rat cardiomyocytes K^+ Channel Blocker-Evoked Signals in Cardiomyocyte"J AM Coll Caridol. 38. 1554-1563 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Motoaki Sano, Keiichi Fukuda et al.: "ERK and p38MAPK. but not NF-κB, are critically involved in reactive oxygen species-mediated induction of IL-6 by angiotensin II in cardiac fibroblasts"Cire Res. 89. 661-669 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Daihiko Hakuno, Keiichi Fukuda et al.: "Bone marrow-derived cardiomyocytes (CMG cell) expressed functionally active adrenergic and muscarinic receptors"Circulation. 105. 380-386 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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