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Research regarding oxygen-sensitive K channel existing in the ductus arteriosus.

Research Project

Project/Area Number 13470214
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Embryonic/Neonatal medicine
Research InstitutionTokyo Women's Medical University

Principal Investigator

NAKANISHI Toshio  Tokyo Women's Medical University, Dep. Of Medicine, Associate Professor, 医学部, 助教授 (90120013)

Co-Investigator(Kenkyū-buntansha) AIBA Sumi  Tokyo Women's Medical University, Dep. Of Medicine, Assistant Professor, 医学部, 講師 (80138891)
MATSUOKA Rumiko  Tokyo Women's Medical University, Dep. Of Medicine, Assistant Professor, 医学部, 講師 (50120051)
HAGIWARA Nobuhisa  Tokyo Women's Medical University, Dep. Of Medicine, Associate Professor, 医学部, 助教授 (00180802)
HAGANA Emiko  Tokyo Women's Medical University, Dep. Of Medicine, Assistant Professor, 医学部, 助手
NAKAZAWA Makoto  Tokyo Women's Medical University, Dep. Of Medicine, Professor, 医学部, 教授 (10075567)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥14,700,000 (Direct Cost: ¥14,700,000)
Fiscal Year 2002: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥11,200,000 (Direct Cost: ¥11,200,000)
KeywordsDuctus Arteriosus / K channel / Oxygen / Cloning of K channel / クローニング / 遺伝子
Research Abstract

Establishment of cDNA of ductus arteriosus isolated from pig :
Ductal tissue, aorta, pulmonary artery was isolated from 140 newborn pigs immediately after birth. Total RNA and then poly A RNA was isolated from the ductus, aorta, and pulmonary artery. cDNA was made from the RNA using RT-PCR. The cDNA was inserted into vector and cDNA library was established. The length of the inserted DNA was between 1.7 to 2.7 kb.
Cloning Kv using the cDNA library :
The cDNA library of pig ductus arteriosus was screened using Kv probes obtained from rat, mouse, and human beings. Kv 1, Kv 2, and Kv9.3 were cloned from the ductus arteriosus.
Screening of the cDNA library using homology-based PCR :
Primer was made using conserved part of Kv genes and PCR was performed using cDNA as a template. PCR products were compared between the ductus, aorta, and pulmonary artery. PCR products amplified only in the ductus was chosen and cloned. Kv families expressed in the ductus were cloned and they belong to 1.5, 2.1 and 2.2 family.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Wu G, Shem J, Momma K, Nakanishi T: "Effect of Vitamin A on contraction of the ductus arteriosus in fetal rat"Pediatric Research. 49. 747-754 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 中西敏雄: "心臓と血管の収縮弛緩制御機構の発達"日本小児科学会雑誌. 105. 1283-1292 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shem J, Nakanishi T, Hong Gu, et al.: "The role of endothelin in oxygen-induced contraction of the ductus arteriosus in rabbit and rat fetuses"Heart Vessels. 16. 181-188 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 沈菁, 中西敏雄, 中沢誠: "ラット胎仔動脈管におけるATP感受性Kチャンネル"東京女子医科大学学会誌. 72. 521-527 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shem J, Katsube Y, Wu G, Mommma K, Nakanishi T: "ATP-sensitive pottasium channel in the ductus arteriosus of fetal rat"Pediatric Research. (In submission). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Wu G, Shen J, Momma K, Nakanishi T: "Effect of vitamin A on contraction of the ductus arteriosus in fetal rat."Pediatric Research. 49. 747-754 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakanishi T: "Developmental changes in contractile system of cardiovascular system."J. of Japan Pediatric Society. 105. 1283-1292 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shen J, Nakanishi T, Gu H, et al.: "The role of endothelin in oxygen-induced contraction of the ductus arteriosus in rabbit and rat fetuses."Heart & Vessels. 16. 181-188 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shen J, Nakanishi T, Nakazawa M: "ATP-sensitive K channel in the rat ductus"J. of Tokyo Women's Medical University. 72. 521-527 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shen J, Katsube Y, Wu G, Momma K, Nakanishi T: "ATP-sensitive K channel in the ductus arteriosus of fetal rat."Pediatric Research. (in submission).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary

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Published: 2001-04-01   Modified: 2016-04-21  

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