| Project/Area Number |
13470216
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | Fukui Medical University |
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Principal Investigator |
MIYAMOTO Kaoru Fukui Medical University, Faculty of Medicine, Professor, 医学部, 教授 (30125877)
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| Co-Investigator(Kenkyū-buntansha) |
MIZUTANI Tetsuya Fukui Medical University, Faculty of Medicine, Research Associate, 医学部, 助手 (90322734)
YAMADA Kazuya Fukui Medical University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (20263238)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2001: ¥5,100,000 (Direct Cost: ¥5,100,000)
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| Keywords | reproductive tissue specific factor / gonadotropin inducible / activation of transcription / zinc-finger structure / 卵巣 / 転写因子 / ゴナドトロピン |
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| Research Abstract |
In this study we have tried to identify ovarian target genes for GIOT (Gonadotropin Inducible Ovarian Transcription factor) in order to clarify roles of GIOT in ovarian functions. GIOT seems to bind upstream regulatory regions of target genes for GIOT, and we have already cloned various ovarian genes that are strongly induced by gonadotropin treatment, such as SR-BI (Scavenger Receptor class B type I), NGF-IA (egr-1), StAR (Steroidogenic Acute Regulatory protein), LH-receptor, 20-beta HSD, 3-beta HDS, P450scc, etc. We have checked effects of GIOT on the transcription of these genes. By using luciferase assay system, effects of GIOT on the transcriptional activity of these gonadotropin inducible genes. In addition, we have tried to identify GIOT interacting proteins in rat granulosa cells. As a result, we have cloned a novel gene that encodes a HMG-box type transcriptional regulator, and we named it GCX-1. GCX-1 is found to be expressed exclusively in reproductive organs. Identification of a novel transcription factor specific to reproductive tissues is a fruitful result of this work.
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