| Project/Area Number |
13470258
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Nagoya City University |
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Principal Investigator |
MANABE Tadao Nagoya City University, Graduate School of Medical Sciences, Professor, 大学院・医科学研究科, 教授 (80127141)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Mikinori Nagoya City University, Graduate School of Medical Sciences, Research Associate, 大学院・医科学研究科, 助手 (20305551)
OKADA Yuji Nagoya City University, Graduate School of Medical Sciences, Research Associate, 大学院・医科学研究科, 助手 (10305550)
TAKEYAMA Hiromitsu Nagoya City University, Graduate School of Medical Sciences, Associate Professor, 大学院・医科学研究科, 助教授 (00216946)
SAWAI Hirozumi Nagoya City University, Graduate School of Medical Sciences, Research Associate, 大学院・医科学研究科, 助手 (40336681)
FUNAHASHI Hitoshi Nagoya City University, Graduate School of Medical Sciences, Research Associate, 大学院・医科学研究科, 助手 (10347411)
赤毛 義実 名古屋市立大学, 医学部, 助教授 (90264720)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥14,200,000 (Direct Cost: ¥14,200,000)
Fiscal Year 2003: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 2002: ¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 2001: ¥5,000,000 (Direct Cost: ¥5,000,000)
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| Keywords | Pancreatic / Nerve invasion / GDNF / RET / Integrin / Tyrosine kinase |
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| Research Abstract |
We report the results that we acquired till now as follows.
1.The expression of GDNF and RET in the intra-and extra-pancreatic nerve plexus was observed by immunohistochemical analysis in resected specimens. It was also proved that pancreatic cancer cells and peri-pancreatic tissues excrete GDNF.
2.The role of NF-κB in signaling pathway via GDNF-RET was investigated.
(1)MG-132, proteasome inhibior, inhibited the proliferation of pancreatic cancer cells in dose-dependent manner.
(2)The invasion cell number and the NF-κB activity were increased by GDNF stimulation. But in the presence of MG132 or I-κB activity were significantly suppressed and remained suppressed after GDNF stimulation.
(3)GDNF upregulates the expression and enzymatic activity of MMP-9, and this may promote pancreatic cancer invasion to the extracellular matrixes.
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