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Genetic aberrations in the metastatic process and its control -mouse sarcoma and human sarcoma cells-

Research Project

Project/Area Number 13470306
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionTOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY

Principal Investigator

KANAMORI Masahiko  Toyama Medical and Pharmaceutical University, Faculty of Medicine, Department of Orthopedics, Associate Professor, 医学部, 助教授 (20204547)

Co-Investigator(Kenkyū-buntansha) OHMORI Kazuo  Toyama Medical and Pharmaceutical University, Faculty of Medicine, Department of Orthopedics, Assistant Professor, 附属病院, 助手 (80303238)
KAWAGUCHI Makoto  Toyama Medical and Pharmaceutical University, Faculty of Medicine, Department of Pathology, Assistant Professor, 医学部, 助手 (50204699)
GEJO Ryuichi  Toyama Medical and Pharmaceutical University, Faculty of Medicine, Department of Orthopedics, Assistant Professor, 医学部, 助手 (00359712)
Project Period (FY) 2001 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥14,500,000 (Direct Cost: ¥14,500,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2003: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2001: ¥9,700,000 (Direct Cost: ¥9,700,000)
Keywordsfibrosarcoma / metastasis / gene analysis / sarcoma / genomic hybridization / metastasisi
Research Abstract

Genomic analysis of the mouse sarcoma and its differentiation and apoptotic therapy
Genomic analysis of a high-metastatic clone established from poorly differentiated murine sarcoma (HM-RCT), in which a tumor was spontaneously developed in a C3H/He mouse, was used. HM-RCT expressed enhanced POU domain (class 2, associating factor 1), adenylate cyclase 7, procollagen type III (a), A kinase anchor protein 4 and Ehm (expressed on high-metastatic cells) and 11 expressed sequence tags (ESTs), compared with the original clone of RCT. However, 18 specific genes and 14 ESTs were underexpressed in HM-RCT. Moreover, we investigated the effects of angiogenesis inhibitor TNP-470, dibutyryl cyclic AMP and hyperthermia on tumor growth and differentiation for the mouse sarcoma cells.
Genomic analysis of the human sarcoma
Genetic instability analyzed by comparative genomic hybridization method (CGH) were identified in human sarcomas. Seven clones showed high-level amplification in all osteosarcomas. They were 1Qtel10 (1qtel), WHSC1(4p16.3), CCND3(6p21), MTAP(9p21.3), INSR(19p13.2), CCNE1(19q12), and PCNT2(21qtel). Of these clones, CCND3 and MTAP also showed high-level amplification in aggressive giant cell tumors, whereas 1Qtel10 and MTAP showed high amplification in chondrosarcoma. NRSA(1p13.2) and RAF1(3q25), which were commonly low in aggressive bone tumors, were lost in osteosarcomas. Thus, the present pilot study suggests that array-based genome-wide CGH could powerful means to detect genetic instability and gene aberrations that are reflected to the progression and outcome of human sarcomas.

Report

(5 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (15 results)

All 2005 2004 2003 2001 Other

All Journal Article (11 results) Publications (4 results)

  • [Journal Article] ZFH4 protein is expressed in many neurons of developing rat brain2005

    • Author(s)
      Kanamori, et al.
    • Journal Title

      J.Comparative Neurology 482

      Pages: 33-49

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Currattage and radiotherapy in sacral giant cell tumor2005

    • Author(s)
      Kanamori, et al.
    • Journal Title

      J.Orthop.Surg 13(in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Cystic lymphangiomas of the cauda equina2004

    • Author(s)
      Kanamori, et al.
    • Journal Title

      The Spine Journal 4

      Pages: 357-359

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Giant cell tumor of the sternum2004

    • Author(s)
      Kanamori, et al.
    • Journal Title

      J.Orthop.Sci 9

      Pages: 175-177

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effects of hyperthermia and differentiation on cultured Dunn osteosarcoma cells2003

    • Author(s)
      M.Kanamori, et al.
    • Journal Title

      Cancer Detection and Prevention 27

      Pages: 76-81

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] CGH法による腫瘍染色体解析技術について2003

    • Author(s)
      金森昌彦
    • Journal Title

      別冊整形外科 43

      Pages: 79-83

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 肉腫肺転移のメカニズムとその治療について-マウスRCT肉腫高肺転移株を用いた一連の研究から-2003

    • Author(s)
      金森昌彦ほか
    • Journal Title

      別冊整形外科 43

      Pages: 44-48

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The mechanism and treatment of lung metastasis of sarcoma ; from the study of high metastatic clone of RCT2003

    • Author(s)
      M.Kanamori, et al.
    • Journal Title

      Bessatsu Seikeigeka (in Japanese) 43

      Pages: 44-48

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cytogenetic analysis of tumor by the technique of comparative genomic hybridization (CGH)2003

    • Author(s)
      M.Kanamori
    • Journal Title

      Bessatsu Seikeigeka (in Japanese) 43

      Pages: 79-83

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Extracopies of chromosomes 7, 8, 12, 19, and 21 are recurrent in adamantinoma.2001

    • Author(s)
      M.Kanamori, et al.
    • Journal Title

      Journal of Molecular Diagnostics 3

      Pages: 16-20

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cytogenetic analysis and review of the literature in adamantinoma and osteofibrous dysplasia2001

    • Author(s)
      M.Kanamori
    • Journal Title

      Cytometry Research 11

      Pages: 35-42

    • NAID

      10026898772

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] 金森 昌彦: "CGH法"整形外科. 53. 578 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 金森 昌彦 ほか: "肉腫肺転移のメカニズムとその治療-マウスRCT肉腫高肺転移株を用いた一連の研究から-"別冊整形外科. 43. 44-48 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 金森 昌彦: "CGH法による腫瘍染色体解析技術について"別冊整形外科. 43. 79-83 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kanamori M., et al.: "Effects of hyperthermia and differentiation on cultured Dunn osteosarcoma cells"Cancer Detection and Prevention. 27. 76-81 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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