| Project/Area Number |
13470344
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Gunma University |
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Principal Investigator |
MINEGISHI Takashi Medicine Professor and Chairman, 医学部, 教授 (00209842)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Yumiko Medicine Associate, 医学部, 助手 (70261857)
NAKAMURA Kazuto Medicine Assistant Professor, 医学部, 講師 (60332558)
ITOU Masahiro Medicine Assistant Professor, 医学部, 講師 (20282402)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥12,500,000 (Direct Cost: ¥12,500,000)
Fiscal Year 2002: ¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 2001: ¥7,200,000 (Direct Cost: ¥7,200,000)
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| Keywords | FSH receptor / LH receptor / TGF-β / Activin / Follistatin / StAR / Granulosa cell / フォリスタチン / ステロイド産生 |
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| Research Abstract |
We investigated the effect of TGF-β on the expression of FSH and LH receptor expression in the rat granulasa cells. TGF-β increased FSH-R mRNA levels with a maximum of about a 2-fold in a dose-dependent manner compared to the control. In addition, treatment with FSH produced a substantial increase in LH-R mRNA, and concurrent treatment with increasing concentration of TGF-β brought about dose-dependent increases in FSH-induced LH-R mRNA. FSH significantly enhanced the activity of FSH and LH receptor 5'-flanking region, but treatment with TGF- β did not significantly influence the activity induced by FSH. On the other hand, the decay curves for FSH-R mRNA and LH-R mRNA transcripts in granulosa cells showed a significant increase in half-life after the addition of TGF-β.
TGF-β also stimulated the expression of follistatin mRNA accumulation in a dose- and time- dependent manner. Follistatin did not affect TGF-β-induced FSH-R mRNA. Therefore, the profile of the TGF-β effect on FSH-R mRNA and LH-R mRNA may be due to the increased stability of both receptor mRNAs and insensitivity of the follistatin.
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