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The development of the vascular inducible cultured skin substitute (VICSS).

Research Project

Project/Area Number 13470381
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plastic surgery
Research InstitutionTokai University

Principal Investigator

TANINO Ryuzaburo  Tokai University, School of Medicine, Professor, 医学部, 教授 (50051595)

Co-Investigator(Kenkyū-buntansha) INOKUCHI Sadaki  Tokai University, School of Medicine, Professor, 医学部, 教授 (60160008)
NAKAZAWA Hiroe  Tokai University, School of Medicine, Professor, 医学部, 教授 (20110885)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥7,900,000 (Direct Cost: ¥7,900,000)
Fiscal Year 2003: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2002: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2001: ¥3,000,000 (Direct Cost: ¥3,000,000)
Keywordscultured skin / vascular inductio / angiogenesis / gene transfer
Research Abstract

Growth factors such as vascular endothelial growth factor (VEGF) increase in the transplantation floor of cultured skin substitute (CSS) to stimulate angiogenesis. VEGF in the culture supematant in fibrin types of CSS were measured by EHSA, and VEGF mRNA was determined semiquantitatively by RT-PCR after two days of incubation. Furthermore CSS were implanted to athymic mice. VEGF concentration in the fibrin-cultured supematant was 84.3±11.8 pg/mi, whereas it was 27.8±4.68 pg/ml in the ease of the collagen substrate. The relative levels of VEGF mRNA were 1.088±0.100 and 0.698±0.226, respectively. In in vivo-transplantion, the fibrin-type cultured skin substitute showed an excellent "take" on the wound bed, and a normally proliferating keratinocyte layer with emergence of vascular endothelial cells in the transplanted floor was seen three days
To add the ability of angiogenesis to the CSS, gene transfer such as VEGE was planed In order to develop gene induction using virus vector, we chose the Lentivirus. And to examine the effect of gene expression level, Green Fluorescent Protein (GFP) was used as a reporter gene in vitro and used E. coli beta-galactosidase (LacZ) in vivo. Both reporter gene were well transfened to human nonnal Keratinocytes. It conduded lentivirous is effective to introduce the vascular endothelial growth factor to the human kemtinocytes in order to develop the Vascular Inducible Cultured Skin Substitute (VICSS)

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] Nakazawa, H et al.: "Nitrotyrosine formation and its role in various pathological conditions."Free Rad Res. 33. 771-784 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hirabayashi H, Takizawa S, Fukuyama N, Nakazawa H: "N-methyl-D-aspartate receptor antagonist reduces nitrotyrosine formation in caudate-putamen in rat focal cerebral ischemia-reperfusion."Neurosci Lett. 299. 159-161 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Halejcio-Delophont P, Hoshiai K, Fukuyama N, Nakazawa H: "No evidence of NO-induced damage in potential donor organs after brain death"J Heart Lung Transplant. 20. 71-79 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Xi-Lin Niu, Xia Yang, Hoshiai K, Tanaka K, Sawamura S, Koga Y, Nakazawa H: "Inducible nitric oxide synthase deficiency does not affect susceptibility of mice to atherosclerosis but increases collagen content in lesions"Circulation. 103. 1115-1120 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Abu-Soud H.M., Ichimori K, Nakazawa H, Stuehr D.J.: "Regulation of Inducible Nitric Oxide Synthase by Self-Generated NO"Biochemistry. 40. 6876-6881 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Shimizu, S.Koide, J.N.Yoshimura, H.Nakazawa: "Hyperthyroidism and the management of atrial fibrillation."Thyroid. 12. 489-493 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Takizawa, Y.Aratani, N.Hirabayashi, H.Nakazawa: "Deficiency of myeloperoxidase increases infarct volume and nitrotyrosine formation in mouse brain."J Cereb Flow Metab. 22. 50-54 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 北條元治, 猪口貞樹, 宮坂宗男, 服部禎子, 谷野隆三郎: "東海大学病院における熱傷統計"熱傷. 28巻2号. 19-28 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Hojo, S.Inokuchi, R.Tanino, H.Nakazawa: "Induction of Vascular Endothelial Growth Factor (VEGF) by Fibrin as a Dermal Substrate for Cultured Skin Substitute"Plastic and Reconstructive Surgery. 111. 1638 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 北條元治, 宮坂宗男, 谷野隆三郎: "培養皮膚モデルにおける血管増殖因子の検討"第9回ケロイド・肥厚性瘢痕研究会. 53-59 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakazawa, H et al.: "Nitrotyrosine formation and its role in various pathological conditions."Free Rad Res. 33. 771-784 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hirabayashi H, Takizawa S, Fukuyama N, Nakazawa H: "N-methyl-D-aspartate receptor antagonist reduces nitrotyrosine formation in caudate-putamen in rat focal cerebral ischemia-reperfusion."Neurosci Lett. 299. 159-161 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Halejcio-Delophont P, Hoshiai K, Fukuyama N, Nakazawa H: "No evidence of NO-induced damage in potential donor organs after brain death"J Heart Lung Transplant. 20. 71-79 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Xi-Lin Niu, Xia Yang, Hoshiai K, Tanaka K, Sawamura S, Koga Y Nakazawa H: "Inducible nitric oxide synthase deficiency does not affect susceptibility of mice to atherosclerosis but increases collagen content in lesions"Circulation. 103. 1115-1120 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Abu-Soud H.M., Ichimori K, Nakazawa H, Stuehr D.J.: "Regulation of Inducible Nitric Oxide Synthase by Self-Generated NO"Biochemistry. 40. 6876-6881 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Shimizu, S.Koide, J.N.Yoshimura, H.Nakazawa: "Hyperthyroidism and the management of atrial fibrillation."Thyroid. 12. 489-493 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Takizawa, Y.Aratani, N.Hirabayashi, H.Nakazawa: "Deficiency of myeloperoxidase increases infarct volume and nitrotyrosine formation in mouse brain."J Cereb Flow Metab. 22. 50-54 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Hojo, S.Inokuchi, R.Tanino, et al.: "Burn statistics at the Tokai University Hospital for the past 5 years"Jpn J Burn. 28. 19-28 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Hojo, S.Inokuchi, R.Tanino, H.Nakazawa: "Induction of Vascular Endothelial Growth Factor (VEGF) by Fibrin as a Dermal Substrate for Cultured Skin Substitute"Plast Reconstr Surg. 111. 1638-1645 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Hojo, M.Miyasaka, R.Tanino: "The examination of Vascular Endothelial Growth Factor in the cultured skin models"9th Annual meeting of the keroid and hypeitrophic scar. 9. 53-59 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 北條元治, 宮坂宗男, 谷野隆三郎: "培養皮膚モデルにおける血管増殖因子の検討"第9回ケロイド・肥厚性瘢痕研究会. 53-59 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Shimizu, S.Koide, J.N.Yoshimura, K.Kiminori, K.Ito, H.Nakazawa: "Hyperthyroidism and the management of atrial fibrillation"Thyroid. 12. 489-493 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.Takizawa, Y.Aratani, N.Hirabayashi, H.Nakazawa: "Deficiency of myeloperoxidase increases infarct volume and nitrotyrosine formation in mouse brain"J Cereb Flow Metab. 22. 50-54 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Hojo, S.Inokuchi, R.Tanino, H.Nakazawa: "Induction of Vascular Endothelial Growth Factor (VEGF) by Fibrin as a Dermal Substrate for Cultured Skin Substitute"Plastic and Reconstructive Surgery. 111(In press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 北條元治, 猪口貞樹, 宮坂宗男, 服部禎子, 谷野隆三郎: "東海大学病院における熱傷統計"熱傷. 28. 19-28 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakazawa, H et.al: "Nitrotyrosine formation and its role in various pathological conditions"Free Rad Res. 33. 771-784 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hirabayashi H, Takizawa S, Fukuyama N, Nakazawa H: "N-methyl-D-aspartate receptor antagonist reduces nitrotyrosine formation in caudate-putamen in rat focal cerebral is chemia-reperfusion"Neurosci Lett. 299. 159-161 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Halejcio-Delophont P, Hoshiai K, Fukuyama N, Nakazawa H: "No evidence of NO-induced damage in potential donor organs after brain death"J Heart Lung Transplant. 20. 71-79 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Xi-Lin Niu, Xia Yang, Hoshiai K, Tanaka K, Sawamura S, Koga Y, Nakazawa H: "Inducible nitric oxide synthase deficiency does not affect susceptibility of mice to atherosclerosis but increases collagen content in lesions"Circulation. 103. 1115-1120 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Abu-Soud H.M., Ichimori K, Nakazawa H, Stuehr D.J.: "Regulation of Inducible Nitric Oxide Synthase by Self-Generated NO"Biochemistry. 40. 6876-6881 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 北條元治, 猪口貞樹, 宮坂宗男, 服部禎子, 谷野隆三郎: "東海大学病院における熱傷統計"熱傷. 28巻2号(In press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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