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Molecular biological properties of oral treponemes and their outer membrane extracts

Research Project

Project/Area Number 13470390
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionAsahi University

Principal Investigator

OGAWA Tomohiko  School of Dentistry, Department of Oral Microbiology, Professor, 歯学部, 教授 (80160761)

Co-Investigator(Kenkyū-buntansha) HASHIMOTO Masahito  School of Dentistry, Department of Oral Microbiology, Instructor, 歯学部, 助手 (30333537)
ASAI Yasuyuki  School of Dentistry, Department of Oral Microbiology, Instructor, 歯学部, 助手 (30329487)
山本 浩代  朝日大学, 歯学部, 助手 (50329497)
隅田 泰生  大阪大学, 大学院・理学研究科, 助教授 (70179282)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥11,100,000 (Direct Cost: ¥11,100,000)
Fiscal Year 2002: ¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 2001: ¥6,400,000 (Direct Cost: ¥6,400,000)
KeywordsOral treponema / Treponema medium / Outer membrane / Human gingival eptithelial cell / Toll-like receptor / Periodontal disease / Immunobiological activity / Cytokine / ヒト歯肉上皮細胞
Research Abstract

Oral treponemes are considered to be important in the development and progression of periodontal diseases. We investigated the recognition and activation mechanisms of human gingival epithelial cells (HGEC) with the oral treponemes Treponema denticola, Treponema vincentii, and Treponema medium, and their outer membrane extracts (OMEs). T. vincentii and T. medium but not T. denticola produced IL-8 in an HGEC culture. Further, all 3 treponemes induced IL-8 Mrna expression and NF-Kb activation in HGEC. Among them, T. denticola especially exhibited trypsin- and chymotrypsin- like protease activities, and the addition of chymostatin, a chymotrypsin protease inhibitor, resulted in detectable IL-8 production by HGEC cultured with T. denticola. Additionally, IL-8 Mrna expression in HGEC cultured with the 3 treponemes and their OMEs was definitely inhibited by the mouse anti-human Toll-like receptor (TLR) 2 monoclonal antibody, TL2.1. These findings suggest that oral treponemes and their OMEs activate human gingival epithelial cells through TLR2

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Toshihiko Umemoto: "Chemotaxis of oral treponemes toward sera and albumin of rabbits"Microbiology and Immunology. 45. 571-577 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yasuyuki Asai: "Detection and quantification of oral treponemes in subgingival plaque by real-time PCR"Journal of Clinical Microbiology. 40. 3334-3340 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yasuyuki Asai: "Oral treponemes and their outer membrane extracts activate human gingival epithelial cells through Toll-like receptor 2"Infection and Immunity. 71. 717-725 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Umemoto, T. et al.: "Chemotaxis of oral treponemes toward sera and albumin of rabbit"Microbiol. And Immunol. 45(8). 571-577 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Asai, Y. et al.: "Distribution and quantification of oral treponemas in subgingival plaque using real-time PCR"J. Clin. Microbiol. 40(9). 3334-3340 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Asai, Y. et al.: "Oral treponemes and their outer membrane extracts activate human gingival epithelial cells through Toll-like receptor 2"Infect. Immun. 71(2). 717-725 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yasuyuki Asai: "Detection and quantification of oral treponemes in subgingival plaque by real-time PCR"Journal of Clinical Microbiology. 40・9. 3334-3340 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yasuyuki Asai: "Oral treponemes and their outer membrane extracts activate human gingival epithelial cells through Toll-like receptor2"Infection and Immunity. 71・2. 717-725 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yasuyuki Asai: "Bacterial fimbriae and their peptides activate human gingival epithelial cells through Toll-like receptor 2"Infection and Immunity. 69・12. 7387-7395 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yasuo Suda: "Chemical structure and biological activity of a lipid A component from Helicobacter pylori strain 206"Journal of Endotoxin Research. 7・2. 95-104 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Toshihiko Umemoto: "Chemotaxis of oral Treponemes toward sera and albumin of rabbit"Microbiology and Immunology. 45・8. 571-577 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 小川 知彦: "Porphyromonas gingivalis合成リピドAに対するTLR欠損マウス由来歯肉線維芽細胞の認識機構"エンドトキシン研究. 4. 73-80 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 朝井 康行: "低毒性リピドAの構造と機能"エンドトキシン研究. 4. 97-101 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 小川 知彦: "LPSを認識するTLR"細胞. 33・7. 12-15 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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