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With the aim of expansion of the application of dental implants for the aged person and the patient who have systemic disease : the development of dental implants combined with the cell tranfected gene of bone related protein

Research Project

Project/Area Number 13470407
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 補綴理工系歯学
Research InstitutionHokkaido University

Principal Investigator

YOKOYAMA Atsuro  Hokkaido University, Hospital, Assi.Prof., 病院, 講師 (20210627)

Co-Investigator(Kenkyū-buntansha) YASUDA Motoaki  Hokkaido Univ., Grad.School of Dental Medicine, Asso.Prof., 大学院・歯学研究科, 助教授 (90239765)
SHINDOH Masanobu  Hokkaido Univ., Grad.School of Dental Medicine, Asso.Prof., 大学院・歯学研究科, 助教授 (20162802)
HIGASHINO Humihiro  Hokkaido Univ., Grad.School of Dental Medicine, Instructor, 大学院・歯学研究科, 助手 (50301891)
YAMAMOTO Akiko  National Institute for Materials Science, Biomaterial Center, Senior Researcher, 材料研究所・生体材料研究チーム, 主任研究員
HANAWA Takao  National Institute for Materials Science, Biomaterial Center, Team Leader., 材料研究所・生体材料研究チーム, チームリーダー
川崎 貴生  北海道大学, 大学院・歯学研究科, 教授 (90002229)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥12,700,000 (Direct Cost: ¥12,700,000)
Fiscal Year 2003: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2002: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2001: ¥6,200,000 (Direct Cost: ¥6,200,000)
Keywordsosteopontin / osteonectin / gene transfection / daibetes / dental implant / bone marrow cell / osteoblast / osseointegration / オステオカルシン / デキサメサゾン / インプラント / 細胞接着率 / 細胞接着強度 / 骨髄幹細胞 / インプテント / チタン / 細胞接着能
Research Abstract

The purpose of this study was expansion of the application of dental implants for sparse bone of the aged person and the patient who have systemic disease. Firstly, we investigate the adhesive potential of exogeneous osteopontin(OP) and osteonectin(ON) expressing osteoblastic cells that we have established. The expression of exogeneous OP and ON was confirmed by Western blotting. Parental Saos2, Saos2-puro, Saos2-pcDNA3, and Saos2-OP and Saos2-ON were seeded on the polystyrene dish at a density of 1.5 x 10^5 cells in serum-free DMEM. The cell adhesion rates(CAR) were counted after 6, 12 and 24 hours of incubation. Also, the cell adhesive shear force (F) and cell adhesive area (A) were measured in serum-free DMEM after 24 hours. The CAR of Saos2-OP was higher than those of parental cells (p<0.05), and that of Saos2-ON was lower than those of parental cells (p<0.05). F/A of Saos2-OP had higher than the parental cells, though no significance was observed in the difference between them. Th … More ese results suggest that human OP is a potent enhancer of osteoblast-like cell adhesion. Secondary, we investigated the effect of diabetes on bone tissue around endosseous implants after osseointegration. Titanium implants were inserted in the femora of rats. At 8 weeks after implantation, diabetes was induced by intra peritoneal injection of streptozotocin. Histopathological and histomorphometric evaluations were carried out. There was no effect of diabetes on the ratio of bone-implant contact, while the amount of newly formed bone in the control group was greater than that of the diabetic group. Next, we developed dental implants combined with the bone marrow cells. Undifferentiated bone marrow cells were cultured on dental implants with dexamethasone for 2 weeks. Dental implants with cultured bone marrow cells were implanted into subcutaneous tissue in rats. Histopathogical investigation and analysis by RT-PCR were done. At 4 weeks after surgery, bone tissue was observed on the surface of dental implants and expression of mRNA of osteocalcin was detected by RT-PCR. These results suggested that the dental implants combined with the cells transfected gene of bone protein were effective on the sparse bone of the aged person and the patient who have systemic disease. Less

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (12 results)

All 2004 2003 2002 Other

All Journal Article (9 results) Book (1 results) Publications (2 results)

  • [Journal Article] 各種顕微鏡および発光分光分析を用いた摘出インプラント周囲組織中のチタンの分析2004

    • Author(s)
      横山敦郎, 松野浩宜, 川崎貴生, 水越孝典, 石川誠, 戸塚靖則, 野田坂佳伸, 宇尾基弘, 亘理文夫, 向後隆男
    • Journal Title

      北海道歯学雑誌 25

      Pages: 330-338

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Analysis of titanium in the tissue surrounding retrieved dental implants by microscopy and emission spectrochemical anlysis2004

    • Author(s)
      Yokoyama A, Matsuno H, Kawasaki T, Mizukoshi T, Ishikawa M, Totsuka Y, Nodasaka Y, Uo M, Watari F, Kohgo T.
    • Journal Title

      Hokkaido Journal of Dental Science 25

      Pages: 330-338

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Implant materials and surface treatment2004

    • Author(s)
      Yokoyama A, Saitoh M.
    • Journal Title

      The Journal of Dental Technology, Extra isuue/implant Laboratory 55

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] 生体材料(デンタルインプラント)を目的とした傾斜機能材料の作製と生体反応2003

    • Author(s)
      横山敦郎, 川崎貴生, 亘理文夫
    • Journal Title

      化学工業 54

      Pages: 837-842

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Dental Implant and Biomaterial2003

    • Author(s)
      Atsuro Yokoyama
    • Journal Title

      Proceedings of 15^<th> Sympojium on Functionally Graded Materials

      Pages: 177-182

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Fabrication and tissue response of the functionally graded materials for biomaterials(dental implants)2003

    • Author(s)
      Yokoyama A, Kawasaki T, Watari F.
    • Journal Title

      Kagaku Kohgyo 54

      Pages: 837-842

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Dental implants and biomaterial2003

    • Author(s)
      Yokoyama A.
    • Journal Title

      Proceedings of 15^<th> Symposium on Functionally Graded Materials

      Pages: 177-182

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Osscointegration獲得後のチタンインプラント周囲の骨組織に及ぼすstreptozotocin誘発糖尿病の影響2002

    • Author(s)
      小畑俊剛, 横山敦郎, 山本悟, 川崎貴生, 尾田充孝, 飯塚正, 向後隆男
    • Journal Title

      歯基礎誌 44

      Pages: 19-28

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] The effect of streptozotocin-induced diabetes on bone tissue around endosseous implants after osseointegration2002

    • Author(s)
      Obata T, Yokoyama A, Yamamoto S, Kawasaki T, Oda M, Iizuka T, Kohgo, T.
    • Journal Title

      Japanese Journal of Oral Biology 44

      Pages: 19-28

    • NAID

      110003164523

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Book] インプラント体材料と表面処理,月刊歯科技工別冊インプラントの技工(渡邉文彦, 市川哲雄(編))2004

    • Author(s)
      横山敦郎, 齋藤正恭
    • Total Pages
      1
    • Publisher
      医歯薬出版
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 横山敦郎, 川崎貴生, 亘理文夫: "生体材料(デンタルインプラント)を目的とした傾斜機能材料の作製と生体反応"化学工業. 54・11. 837-842 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 小畑俊剛: "Osseointegration 獲得後のチタンインプラント周囲の骨組織に及ぼす streptozotocin誘発糖尿病の影響"歯科基礎医学会雑誌. 44・1. 19-28 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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