| Project/Area Number |
13470408
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
補綴理工系歯学
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| Research Institution | Tohoku University |
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Principal Investigator |
WATANABE Makoto Graduate School of Dentistry, professor, 大学院・歯学研究科, 教授 (80091768)
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| Co-Investigator(Kenkyū-buntansha) |
HATORI Yosinori Graduate School of Dentistry, assistant, 大学院・歯学研究科, 助手 (40238035)
TAMAZAWA Yoshinori Hospital of School of Dentistry, lecturer, 歯学部附属病院, 講師 (10124603)
KIKUTI Masahiko Graduate School of Dentistry, assistant professor, 大学院・歯学研究科, 助教授 (60195211)
SATOU Chiaki Graduate School of Dentistry, assistant, 大学院・歯学研究科, 助手 (50312591)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥10,400,000 (Direct Cost: ¥10,400,000)
Fiscal Year 2002: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2001: ¥7,500,000 (Direct Cost: ¥7,500,000)
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| Keywords | Stress / Temporomandibular disorders / Occlusion / Bite |
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| Research Abstract |
There is a possibility that the reaction to stress relates to the cause of the illness. Temporomandibular disorders (TMD) are typical symptoms that are thought to be due to muscle fatigue resulting from abnormal masticatory exercise (such as bruxism resulting from stress) or from improper occlusion. However, the mechanisms underlying these events in skeletal muscle have yet to be established. During some earlier experiments on restraint stress in mice, we noticed an interesting type of behavior. When a mouse is put into a narrow cylinder in which it is unable to turn (the front end being partially closed with a thin, colorless plastic strip, and the back end closed with tape), the mouse gnaws away at the plastic strip. The mouse can move back and forth within the cylinder and freely chooses to bite the plastic strip. However, if its tail is fastened to the back end of the cylinder by tape, the mouse cannot reach the strip and therefore cannot bite it. Using these two variations of the method, we could examine the effects of restraint with or without gnawing (R+G+ and R+G-, respectively). We hypothesize that the effects of gnawing behavior reduces reaction to psychological stress. We examined Whether the gnawing behavior reduced the restraint-induced elevation of serum glucocorticoids or cortisol. As a result R+G+ mice showed a smaller elevation of serum cortisol than that shown by R+G- mice. Thus, R+G- may produce more frustration than R+G+, leading the mouse to produce more glucocorticoids. We measured the total weight of plastic gnawed away up to a given time-point as an index of cumulative gnawing activity (CGA), and the weight of plastic lost per 2-h period as the rate of gnawing. We took notice of time of day on CGA in mice. As a result, CGA was significantly greater in the nighttime hours than in the daytime. In conclusion, our results cold suggest that gnawing behavior reduces reaction to psychological stress.
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