• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analyses of anti-tumor immune responses in the sentinel lymph nodes from the patients with oral

Research Project

Project/Area Number 13470428
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionTokyo Medical and Dental University

Principal Investigator

TAKAHASHI Yuzo  Tokyo Medical and Dental University, Graduate School, Associate Professor, 大学院・医歯学総合研究科, 助教授 (50014329)

Co-Investigator(Kenkyū-buntansha) MARUOKA Yutaka  Tokyo Medical and Dental University, Graduate School, Associate Professor, 大学院・医歯学総合研究科, 助手 (10323726)
AZUMA Miyuki  Tokyo Medical and Dental University, Graduate School, Professor, 大学院・医歯学総合研究科, 教授 (90255654)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥14,300,000 (Direct Cost: ¥14,300,000)
Fiscal Year 2003: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2002: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2001: ¥9,800,000 (Direct Cost: ¥9,800,000)
KeywordsAntitumor immunity / Dendritic cell / Sentinel lymph nodes / Costimulatory molecule / 口腔癌 / 腫瘍免疫
Research Abstract

B7-H1 is one of ligands for an immunoregulatory molecule, PD-1. Our studies using a murine oral squamous cell carcinoma cell line, NRS1 revealed that the endogenously induced B7-H1 on NRS1 cells regulated negatively anti-tumor immune responses through PD-1. In contrast, the B7-H1. expressed at H1gh levels by gene transduction enhanced the antitumor responses in a PD-1-independent manner. These results suggest that B7-H1-mediated immune regulation seems to be very complicated.
On one hand, B7-H1 is also induced on dendritic cells (DC) with B7-DC, wH1ch is another ligand for PD-1. Unlike B7-H1, B7-DC is selectively induced on DC. In the draining lymph nodes after the hapten sensitization, CD86^<high> DC co-expressed B7-DC witH1n the migrating CD11b^+CD11c^+ DC from the skin. The semi-mature DC, wH1ch expressed low levels of CD86 expressed B7-H1 but not B7-DC. The administration of anti-B7-H1 or anti-PD-1 monoclonal antibody at the hapten-sensitization enhanced the responses to the hapten-challenge. These results suggest that the PD-1:B7-H1 pathway is involved in tolerance induction.
The inoculation of H1ghly metastatic NRS1 cells into the tongue induced a rapid expansion of CD11b^<++> large cells in the draining LN. These cells expressed low CD86 and B7-H1 and seem to induce tolerance in the sentinel LN. Our results suggest the possibility that the migrated DC in the sentinel LN caused tolerance induction, wH1ch is dependent upon B7-H1.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Satoru Nuriya: "The role of CTLA-4 in murine contact hypersensitivity"J Invest Dermatol. 116. 764-768 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Seiki Mogi: "The effect of recombinant CD80-Adenovirus and interleukin-12 on generation of sytotoxic T lymphocytes against autologous tumor in patients with oral squamous cell carcinoma"Asian J Oral Maxillofac Surg. 14. 87-94 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Pornpan Youngnak: "Differential binding properties of B7-H1 and B7-DC to programmed death-1 (PD-1)"BBRC. 307. 672-677 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fumihiko Tsushima: "Preferential contribution of B7-H1 to programmed death-1-mediated regulation of hapten-specific allergic inflammatory responses"Eur J Immunol. 33. 2773-2782 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Satoru Nuriya: "The role of CTLA-4 in murine contact hypersensitivity"J Invest Dermatol. 116. 765-768 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Seiki Mogi: "The effect of recombinant CD8O-adenovirus and interleukin-12 on generation of cytotoxic T lymphocytes against autologous tumor in patients with oral squamous cell calcinoma"Asian J Oral Maxillofac Surg. 14. 87-94 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Pornpan Youngnak: "Differential binding properties of B7-H1 and B7-DC to programmed death-1 (PD-1)"BBRC. 307. 672-677 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fumihiko Tsushima: "Preferential contribution of B7-H1 to programmed death-1-mediated regulation of hapten-specific allergic inflammatory responses"Eur J Immunol. 33. 2773-2782 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Pornpan Youngnak: "Differential binding properties of B7-H1 and B7-DC to programmed death-1 (PD-1)"BBRC. 307. 672-677 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Fumihiko Tsushima: "Preferential contribution of B7-H1 to programmed death-1-mediated regulation of hapten-specific allergic inflammatory responses"Eur J Immunol. 33. 2773-2782 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] ポンパン・ユアンナーク: "口腔扁平苔癬における抑制補助シグナル分子PD-1とそのリガンド分子発現"口腔病学会雑誌. 70. 196 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 津島 文彦: "補助シグナル操作による癌免疫治療"Biotherapy. 14. 566-572 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 田中 香衣: "補助刺激分子を標的とした分子治療"医学のあゆみ. 208. 355-361 (2004)

    • Related Report
      2003 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi