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Hard Tissue Regeneration using Gene Activated Matrix

Research Project

Project/Area Number 13470429
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionThe University of Tokyo (2003)
Tokyo Medical and Dental University (2001-2002)

Principal Investigator

ASAHINA Izumi  The University of Tokyo, Institute of Medical Science, Instructor in Donated Research Laboratories, 医科学研究所, 寄付研究部門教員(常勤形態) (30221039)

Co-Investigator(Kenkyū-buntansha) NISITOH Hideki  Tokyo Medical and Dental University, Graduate, Guest Professor, 大学院・医歯学総合研究科, 特任助教授 (00332627)
MARUOKA Yutaka  Tokyo Medical and Dental University, Graduate School, Research Associate, 大学院・医歯学総合研究科, 助手 (10323726)
KASUGAI Shohei  Tokyo Medical and Dental University, Graduate School, Professor, 大学院・医歯学総合研究科, 教授 (70161049)
HONDA Masaki  The University of Tokyo, Institute of Medical Science, Instructor in Donated Research Laboratories, 医科学研究所, 寄付研究部門教員(常勤形態) (70361623)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥14,000,000 (Direct Cost: ¥14,000,000)
Fiscal Year 2003: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2002: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 2001: ¥5,800,000 (Direct Cost: ¥5,800,000)
KeywordsGene activated matrix / Gene transfection / Bone morphogenetic protein / Regenerative medicine / Hard tissue regeneration / 遺伝子活性化基質 / リン酸カルシウム / 遺伝子治療 / 再生医療
Research Abstract

1)Bone Formation by the cells transfected with BMP cDNA
hBMP-2 and 4 cDNAs were cloned from human dental pulp derived cDNA library. pcDNA3 transfected with hBMP-2 or 4 cDNA was transfected into HEK293 cells by lipofectin method. The cells with Three types of scaffolds, collagen sponge, gelatin spoge, and honey-combed collage sponge, were implanted into subcutaneous tissue of mice. The implants were harvested at 2, 4, 8 weeks after operation, and the bone formation were evaluated. As the results, the cells on honey-combed collage sponge formed new bone, whereas the cells on the other scaffolds did not formed bone.
2)Bone Regeneration using Gene Activated Matrix
cDNAs were not transfected effectively into cells when collagen was used as matrix of gene activated matrix. However, the addition of CaP granules to the matrix improved the in vivo gene transfection efficiency. The combination of the plasmid expressing rhBMP-2, collagen and Cap grafted into 5mm bone defect created in rat tibia. The bone defects were regenerated completely with new bone 6 weeks after the implantation. Only 10 ug of plasmid DNA, which was 11100 quantity compared with the conventional way, could regenerate bone.
3)Gene Activated Matrix using PGLA
Collagen is useful biomaterial, but is derived from animal source which has risk of transmitting disease. We found that poly glicoic acid, which is synthetic polymer, could be used as the matrix instead of collagen.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Hosoya M: "Bone with a Vascular Flap Induced from Fat Tissue with the Use of rhBMP-2 in Rats."J Dent.Res. 82(8). 581-584 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kabasawa Y: "Administration of Parathyroid Hormone, Prostaglandin E_2, or 1-alpha,25 Dihydroxyvitamin D_3 Restores the Bone Inductive Activity of rhBMP-2 in Aged Rats."DNA and Cell Biol.. 22(9). 541-546 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Alam MI: "Prefabrication of vascularized bone flap induced by recombinant human bone morphogenetic protein 2 (rhBMP-2)."Int.J.Oral Maxillofac.Surg.. 32(5). 508-514 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoneda S: "The effects of enamel matrix derivative (EMD) on osteoblastic cells in culture and bone regeneration in a rat skull defect."J Periodontal Res.. 38(3). 333-342 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Duarte WR: "S100A4:a novel negative regulator of mineralization and osteoblast differentiation."J Bone Miner Res.. 18(3). 493-501 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 朝比奈泉: "骨形成たんぱく質(Bone Morphogenetic Protein ; BMP)と骨再生"Clinical Calcium. 12(2). 44-49 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] E Marukawa: "Functional reconstruction of the primate mandible using recombinant human bone morphogenetic protein-2."Int.J Oral and Maxillofac Surg.. 31(3). 287-295 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Asahina I: "Reconstruction of the non-human primate mandible using bone morphogenetic protein (BMP) in 5^<th> Asian Conference Oral and Maxillofacial Surgery."Monduzzi Editore, Bologna, Italy. 157 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 朝比奈泉: "再生医療工学の最先端「第II部 再生組織 第4章 頭・頸部 8.顎骨」 監修:筏義人"シーエムシー出版. 265 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hosoya M: "Bone with a Vascular Flap Induced from Fat Tissue with the Use of rhBMP-2 in Rats."J.Dent.Res. 82(8). 581-584 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kabasawa Y: "Administration of Parathyroid Hormone, Prostaglandin E_2, or 1-alpha,25 Dihydroxyvitamin D_3 Restores the Bone Inductive Activity of rhBMP-2 in Aged Rats."DNA and Cell Biol.. 22(9). 541-546 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Alam MI: "Prefabrication of vascularized bone flap induced by recombinant human bone morphogenetic protein 2 (rhBMP-2)."Int.J.Oral Maxillofac.Surg.. 38(3). 508-514 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoneda S: "The effects of enamel matrix derivative (EMD) on osteoblastic cells in culture and bone regeneration in a rat skull defect."J Periodontal Res.. 38(3). 333-342 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Duarte WR: "S100A4: a novel negative regulator of mineralization and osteoblast differentiation."J Bone Miner Res. 18(3). 493-501 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Asahina I: "Bone Morphogenetic Protein and Bone Regeneration"Clinical Calcium. 12(2). 44-49 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] E Marukawa: "Functional reconstruction of the primate mandible using recombinant human bone morphogenetic protein-2."Int.J Oral and Maxillofac Surg. 31(3). 287-295 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Asahina I: "Reconstruction of the non-human primate mandible using bone morphogenetic protein (BMP) in 5^<th> Asian Conference Oral and Maxillofacial Surgery."Monduzzi Editore, Bologna, Italy. 1-4. 157 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Asahina I: "Frontier in BioTissue Engineering (Eds Ikada Y)"CMC Publications, Osaka. 265 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hosoya M, Maruoka Y, Oda M, Asahina I, Ichinose S, Omura K: "Bone with a Vascular Flap Induced from Fat Tissue with the Use of rhBMP-2 in Rats."J Dent.Res. 82(8). 581-584 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kabasawa Y, Asahina I, Gunji A, Omura K: "Administration of Parathyroid Hormone, Prostaglandin E_2,or 1-alpha,25 Dihydroxyvitamin D_3 Restores the Bone Inductive Activity of rhBMP-2 in Aged Rats."DNA and Cell Biol.. 22(9). 541-546 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Alam MI, Asahina I, Seto I, Oda M, Enomoto S.: "Prefabrication of vascularized bone flap induced by recombinant human bone morphogenetic protein 2(rhBMP-2)."Int.J.Oral Maxillofac.Surg.. 32(5). 508-514 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yoneda S, Itoh D, Kuroda S, Kondo H, Umezawa A, Ohya K, Ohyama T, Kasugai S.: "The effects of enamel matrix derivative (EMD) on osteoblastic cells in culture and bone regeneration in a rat skull defect."J Periodontal Res.. 38(3). 333-342 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Duarte WR, Shibata T, Takenaga K, Takahashi E, Kubota K, Ohya K: "S100A4: a novel negative regulator of mineralization and osteoblast differentiation."J Bone Miner Res.2003 Mar. 18(3). 493-501 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Asahina I, Marukawa E, Seto I: "Reconstruction of the non-human primate mandible using bone morphogenetic protein (BMP) in 5^<th> Asian Conference Oral and Maxillofacial Surgery"Monduzzi Editore, Bologna, Italy. 165 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 朝比奈泉, 丸川恵理子, 瀬戸一郎: "骨形成たんぱく質(Bone Morphogenetic Protein ; BMP)と骨再生"Clinical Calcium. 12・2. 44-49 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] E Marukawa, I Asahina, M Oda, I Seto, Md A Imuranul: "Functional reconstruction of the primate mandible using recombinant human bone morphogenetic protein-2"Int. J Oral and Maxillofac Surg.. 31・3. 287-295 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 朝比奈泉: "再生医療工学の最先端「第II部再生組織 第4章頭・頸部 8.顎骨」監修:筏義人"シーエムシー出版(分担). 265 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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