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ESTABLISHMENT AND APPLIANCE OF NEWLY GENE THERAPY FOR DEGENERATIVE ARTHRITIS OF THE TEMPOROMANDIBULAR JOINT

Research Project

Project/Area Number 13470437
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionKyushu University

Principal Investigator

TAKENOSHITA Yasuharu  KYUSHU UNIVERSITY, GRADUATE SCHOOL OF DENTAL SCIENCE, ASS PROP., 大学院・歯学研究院, 助教授 (50117157)

Co-Investigator(Kenkyū-buntansha) NAKAGAWA Kazunori  KYUSHU UNIVERSITY, GRADUATE SCHOOL OF DENTAL SCIENCE, ASS PROP., 大学院・歯学研究院, 講師 (50217668)
ISHIBASHI Hiroaki  KYUSHU UNIVERSITY, GRADUATE SCHOOL OF DENTAL SCIENCE, ASS PROP., 大学院・歯学研究院, 助手 (90254630)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥14,700,000 (Direct Cost: ¥14,700,000)
Fiscal Year 2002: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2001: ¥12,100,000 (Direct Cost: ¥12,100,000)
KeywordsGENE THEAPY / EXTRACELLULAR MATRIX / TMJ / 顎関節 / 基礎破壊酵素
Research Abstract

(1) Examination for the expression of uPA and MMPs stimulated by several cytokines using cultured cells.
The expression of uPA and MMPs was increased by the stimulation with TNF α using cultured cells derived from cartilaginous tissue of TMJ
(2) Examination of transcriptional factor related to cytokines stimulation.
Transcriptional factors, NF-kB and Sp1, were activated by TNF α stimulation at the cultured cells derived from cartilaginous tissue
(3) Establishment of the condition for the gene transfer
We established the condition for the Decoy oligodeoxynucletides targeted to NF-kB and Sp1 using HVJ-liposome method, and decoy ODNs were routinely transferred to 100% of the cells.
(4) Inhibition of the expression of uPA and MMPs by Decoy ODNs transfection
The expression of the uPA and MMPs were inhibited by the transfection of Decoy ODNs to the cultured cells derived from cartilaginous tissue

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Onimaru M, et al.: "FGF-2 stimulates HGF expression, regardless to hypoxia-mediated down regulation in ischemic limb"Circulation Res. 91. 923-930 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shiratuchi T, et al.: "Inhibition of epidermal growth factor-induced invasion by dexamethasone in human squamous cell carcinoma cell lines"J Cell Physiol. 190. 343-348 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Okamura, K: "Immunohistochemical expression of CA19-9 and CA125 in mucoepidermoid and adenoid cystic carcinomas of the salivary gland"Oral Oncology. 38. 244-250 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kurokawa, H, et al.: "Estimation of serum tumor necrosis factor-α and correlation to tumor markers in patients with oral squamous cell carcinoma"Asian J Oral Maxillofac Surg. 14. 148-154 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ishibashi H, et al.: "Hypoxia-induced angiogenesis of cultured human salivary gland carcinoma cells enhances vascular endothelial growth factor production and basic fibroblast growth factor release"Oral Oncol. 37. 77-83 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Onimaru M., et. al.: "FGF-2 stimulates HGF expression, regardless to hypoxia-mediated down regulation in ischemic limb"Circulation Res.. 91. 923-930 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shiratuchi T. et.al.: "Inhibition of epidermal growth factor-induced invasion by dexamethasone in human squamous cell carcinoma cell lines."J Cell Physiol. 190. 343-348 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Okamura K.: "Immunohistochemical expression of CA19-9 and CA125 in mucoepidermoid and adenoid cystic carcinomas of the salivary gland"Oral Oncology. 38. 244-250 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kurokawa H. et. al.: "Estimation of serum tumor necrosis factor- α and correlation to tumor markers in patients with oral squamous cell carcinoma"Asian J Oral Maxillofac Surg. 14. 148-154 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ishibashi H. et. al: "Hypoxia-induced angiogenesis of cultured human salivary gland carcinoma cells enhances vascular endothelial growth factor production and basic fibroblast growth factor release"Oral Oncol. 37. 77-83 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Onimaru.M et al.: "Fibroblast growth factor-2 gene transfer can stimulate hepatocyte growth factor expression irrespective of hypoxia-mediated downregulation in ischemic limbs"Circulation Research. 91. 923-930 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shiratuchi, T., Ishibashi, H.et al.: "Inhibition of epidermal growth factor-induced invasion by dexamethasone and AP-1 decoy in human squamous cell carcinoma cell lines"J Cell Physiol. 93. 340-348 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Okamura, K et al.: "Immunohistochemical expression of CA19-9 and CA125 in mucoepidermoid and adenoid cystic carcinomas of the salivary gland"Oral Oncology. 38. 244-250 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kurokawa, H et al.: "Estimation of serum tumor necrosis factor-α and correlation to tumor markers in patients with oral squamous cell carcinoma"Asian J Oral Maxillofac Surg. 14. 148-154 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ishibashi, H et al.: "Hypoxia-induced angiogenesis of cultured human salivary gland carcinoma cells enhances vascular endothelial growth factor production and basic fibroblast growth factor releases"Oral Oncology. 37. 77-83 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ishibashi H, et al.: "Hypoxia-Induced Angiogenesis of Cultured Human Salivary Gland Carcinoma CellsInvolves Enhancement VEGF Production and bFGF Release"Oral Oncology. 37. 201-206 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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