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Establishment of Post-Proteomic Analysis by the Development of Video-Mass-Scope Method

Research Project

Project/Area Number 13470479
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical pharmacy
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

MASUJIMA Tsutomu  Hiroshima University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (10136054)

Co-Investigator(Kenkyū-buntansha) SHINGAKI Tomoteru  Hiroshima University, Faculty of Medicine, Teaching Associate, 医学部, 教務員 (80314748)
HIRAKAWA Yasuyuki  Hiroshima University, Graduate School of Biomedical Sciences, Assistant Professor, 大学院・医歯薬学総合研究科, 講師 (80238344)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥14,900,000 (Direct Cost: ¥14,900,000)
Fiscal Year 2002: ¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 2001: ¥8,300,000 (Direct Cost: ¥8,300,000)
KeywordsVideo-microscope / Mass Spectrometer / Video-Mass-Scope / Single Cell Mass Spectrometry / Single Cell Analysis / Cell Molecular Dynamism / Image Analysis / ビデオマイクロスコープ / ポストプロテーオーム解析 / ビデオ-マススコープ / 可視化解析 / プロテオーム / ポストゲノム
Research Abstract

1) Development of Sensitive Mass Spectrometer : In order to analyze the secreted molecules from a cell, an ion-trap-TOF mass spectrometer and a TOP mass spectrometer with Nano-spray ionization source were developed.
2) Development of Molecular Trapping Interface : Nano-spray type ion trapping tips were investigated and the tip in which pre-concentrating resins were embedded inside, was developed and applied to pre-concentration of secreted molecules from Mast cells.
3) Development of the Video-Mass-Scope : By combination of the equipments 1. and method 2., the video-mass-scope was developed and applied to the detection of secreted molecules from the cells. On the stimulation of Mast Cells, Histamine and Serotonin were successfully detected by this method. This research was presented in the 123-th Annual Meetings of Japan Pharmaceutical Society.
4) Single Cell MALDI-TOF Mass Spectrometric Analysis : This method was developed and successfully applied to analyze the increase of Histamine contents in a single Mast Cell according to the cell maturation process and the report was published.
5) Development of Pin Fiber Video-Micro-Scope : This scope was also developed in order to investigate a single cell for long time with little irradiation damage to the cell. This method is also found to be useful to apply to the single molecular detection.
The fundamentals and related methodologies were successfully developed by this study and the potentials of these methods were high enough to the direct and simultaneous observation of cell behavior and its dynamic changes in molecular contents.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] 升島 努: "視覚による細胞動態解析と創薬"化学と教育. 2001. 543-547 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] N.Ojima, T.Shingaki, T.Yamamoto, T.Masujima: "Droplet Electro-coupling between capillary electrophoresis and matrix assisted laser desorption ionization time of flight mass spectroscopy and its application"Electrophoresis. 22. 3478-3482 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Shimizu, F.Levi-Scheffer, N.Ojima, T.Shingaki, T.Masujima: "A Single-Cell Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass-Spectroscopic Assay of the Cell-Maturation Process"Anal. Sciences. 18. 107-108 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Hirakawa, M.Suzutoh, H.Ohnishi, T.Shingaki, E.M.Eyring, M.Tokunaga, T.Masujima: "Analysis of the Nano-Kinetic Movement of a Single DNA by a Pin-Fiber Video Scope"Anal. Sciences. 18. 1293-1294 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Suzutoh, Y.Hirakawa, H.Ohnishi, T.Shingaki, E.M.Eyring, M.Tokunaga, T.Masujima: "Nano-Kinetics of Probe-Particles in Solution Visualized by a Pin-Fiber Video Scope"Anal. Sciences. 19. 43-47 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Shimizu, N.Ojima, H.Ohnishi, T.Shingaki, Y.Hirakawa, T.Masujima: "Development of the Single-Cell MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight) Mass-Spectroscopic Assay"Anal. Sciences. 19. 49-53 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 升島努, 尾島典行: "楽しい科学実験 II 試みから想像へ ----侵入者から命を守る----"丸善株式会社. 160 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T. Masujima: "Apllication of Visualization Methods to Cell Dynamic Analysis and Pharmaceutical Developments"Kagaku to Kyouiku (Chemistry and Education). 2001. 543-547 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] N. Ojima, T. Shingaki, T. Yamamoto, T. Masujima: "Droplet Electro-coupling between capillary electrophoresis and matrix assisted laser desorption ionization time of flight mass spectroscopy and its application"Electrophorelsis. 22. 3478-3482 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M. Shimizu, F. Levi-Scheffer, N. Ojima, T. Shingaki, T. Masujjma: "A Single-Cell Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass-Spectroscopic Assay of the Cell-Maturation Process"Anal. Sciences. 18. 107-108 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirakawa, M. Suzutoh, H. Ohnishi, T. Shingaki, E. M. Eyring, M. Tokunaga, T. Masuiima.: "Analysis of the Nano-Kinetic Movement of a Single DNA by a Pin-Fiber Video Scope"Anal. Sciences. 18. 1293-1294 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M. Suzutoh, Y. Hirakawa, H. Ohnishi, T. Shingaki, E. M. Eyring, M. Tokunaga, T. Masujima.: "Nano-Kinetics of Probe-Particles in Solution Visualized by a Pin-Fiber Video Scope"Anal. Sciences. 19. 43-47 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M. Shimizu, N. Ojima, H. Ohnishi, T.Shingaki, Y. Hirakawa, T. Masujima: "Development of the Single-Cell MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight) Mass-Spectroscopic Assay"Anal. Sciences. 19. 49-53 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T. Masujima, N. Ojima, T. Tuda Ed.: "Scientific Experiments II -- Guarding life from invader --"Maruzen. 160 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Shimizu, T.Shingaki, T.Masujima 他: "A Signle-Cell Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass-Spectroscopic Assay of the Cell-Maturation Process"Analytical Sciences. 18. 107-108 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Y.Hirakawa, T.Shingaki, T.Masujima 他: "Analysis of the Nano-Kinetic Movement of a Single DNA by a Pin-Fiber Video Scope"Analytical Sciences. 18. 1293-1294 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Suzuzto, Y.Hirakawa, T.Shingaki, T.Masujima 他: "Nano-Kinetics of Probe-Particles in Solution Visualized by a Pin-Fiber Video Scope"Analytical Sciences. 19. 43-47 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Shimizu, T.Shingaki, Y.Hirakawa, T.Masujima 他: "Development of the Single-Cell MALDI-TOF(Matrix-Assisted Laser Desorption/lonization Time-of-Flight) Mass-Spectroscopic Assay"Analytical Sciences. 19. 49-53 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] N.Ojima, T.Shingaki, T.Masujima et al.: "Droplet Electro-coupling between capillary electrophoresis and matrix assisted laser desorption ionization time of flight mass spectroscopy and its application"Electrophoresis. 22(6). 3478-3482 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Shimizu, T.Shingaki, T.Masujima et al.: "A single-cell matrix-assisted laser desorption/ionization time-of-flight mass-spectroscopic assay of the cell-maturation process"Analytical Sciences. 18(2). 107-108 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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